PSAP (NM_002778) Human Untagged Clone

SKU SC118405

PSAP (untagged)-Human prosaposin (PSAP), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: PSAP
• Synonyms: GLBA; SAP1; SAP2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118405 sequence for NM_002778 edited (data generated by NextGen Sequencing); ATGTACGCCCTCTTCCTCCTGGCCAGCCTCCTGGGCGCGGCTCTAGCCGGCCCGGTCCTT; GGACTGAAAGAATGCACCAGGGGCTCGGCAGTGTGGTGCCAGAATGTGAAGACGGCGTCC; GACTGCGGGGCAGTGAAGCACTGCCTGCAGACCGTTTGGAACAAGCCAACAGTGAAATCC; CTTCCCTGCGACATATGCAAAGACGTTGTCACCGCAGCTGGTGATATGCTGAAGGACAAT; GCCACTGAGGAGGAGATCCTTGTTTACTTGGAGAAGACCTGTGACTGGCTTCCGAAACCG; AACATGTCTGCTTCATGCAAGGAGATAGTGGACTCCTACCTCCCTGTCATCCTGGACATC; ATTAAAGGAGAAATGAGCCGTCCTGGGGAGGTGTGCTCTGCTCTCAACCTCTGCGAGTCT; CTCCAGAAGCACCTAGCAGAGCTGAATCACCAGAAGCAGCTGGAGTCCAATAAGATCCCA; GAGCTGGACATGACTGAGGTGGTGGCCCCCTTCATGGCCAACATCCCTCTCCTCCTCTAC; CCTCAGGACGGCCCCCGCAGCAAGCCCCAGCCAAAGGATAATGGGGACGTTTGCCAGGAC; TGCATTCAGATGGTGACTGACATCCAGACTGCTGTACGGACCAACTCCACCTTTGTCCAG; GCCTTGGTGGAACATGTCAAGGAGGAGTGTGACCGCCTGGGCCCTGGCATGGCCGACATA; TGCAAGAACTATATCAGCCAGTATTCTGAAATTGCTATCCAGATGATGATGCACATGCAA; CCCAAGGAGATCTGTGCGCTGGTTGGGTTCTGTGATGAGGTGAAAGAGATGCCCATGCAG; ACTCTGGTCCCCGCCAAAGTGGCCTCCAAGAATGTCATCCCTGCCCTGGAACTGGTGGAG; CCCATTAAGAAGCACGAGGTCCCAGCAAAGTCTGATGTTTACTGTGAGGTGTGTGAATTC; CTGGTGAAGGAGGTGACCAAGCTGATTGACAACAACAAGACTGAGAAAGAAATACTCGAC; GCTTTTGACAAAATGTGCTCGAAGCTGCCGAAGTCCCTGTCGGAAGAGTGCCAGGAGGTG; GTGGACACGTACGGCAGCTCCATCCTGTCCATCCTGCTGGAGGAGGTCAGCCCTGAGCTG; GTGTGCAGCATGCTGCACCTCTGCTCTGGCACGCGGCTGCCTGCACTGACCGTTCACGTG; ACTCAGCCAAAGGACGGTGGCTTCTGCGAAGTGTGCAAGAAGCTGGTGGGTTATTTGGAT; CGCAACCTGGAGAAAAACAGCACCAAGCAGGAGATCCTGGCTGCTCTTGAGAAAGGCTGC; AGCTTCCTGCCAGACCCTTACCAGAAGCAGTGTGATCAGTTTGTGGCAGAGTACGAGCCC; GTGCTGATCGAGATCCTGGTGGAGGTGATGGATCCTTCCTTCGTGTGCTTGAAAATTGGA; GCCTGCCCCTCGGCCCATAAGCCCTTGTTGGGAACTGAGAAGTGTATATGGGGCCCAAGC; TACTGGTGCCAGAACACAGAGACAGCAGCCCAGTGCAATGCTGTCGAGCATTGCAAACGC; CATGTGTGGAACTAG; ; Clone variation with respect to NM_002778.2;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002778 unedited; TGTTCACCATATTTGTATACGACTCACTATTAGGGCGGCCGCGATTCGGCACGAGGCTGC; GGAGTCAGACGGCGCTATGTACGCCCTCTTCCTCCTGGCCAGCCTCCTGGGCGCGGCTCT; AGCCGGCCCGGTCCTTGGACTGAAAGAATGCACCAGGGGCTCGGCAGTGTGGTGCCAGAA; TGTGAAGACGGCGTCCGACTGCGGGGCAGTGAAGCACTGCCTGCAGACCGTTTGGAACAA; GCCAACAGTGAAATCCCTTCCCTGCGACATATGCAAAGACGTTGTCACCGCAGCTGGTGA; TATGCTGAAGGACAATGCCACTGAGGAGGAGATCCTTGTTTACTTGGAGAAGACCTGTGA; CTGGCTTCCGAAACCGAACATGTCTGCTTCATGCAAGGAGATAGTGGACTCCTACCTCCC; TGTCATCCTGGACATCATTAAAGGAGAAATGAGCCGTCCTGGGGAGGTGTGCTCTGCTCT; CAACCTCTGCGAGTCTCTCCAGAAGCACCTAGCAGAGCTGAATCACCAGAAGCAGCTGGA; GTCCAATAAGATCCCAGAGCTGGACATGACTGAGGTGGTGGCCCCCTTCATGGCCAACAT; CCCTCTCCTCCTCTACCCTCAGGACGGCCCCCGCAGCAAGCCCCAGCCAAAGGATAATGG; GGACGTTTGCCAGGACTGCATTCAGATGGTGACTGACATCCAGACTGCTGTACGGACCAA; CTCCACCTTTGTCCAGGCCTTGGTGGAACATGTCAAGGAGGAGTGTGACCGCCTGGGCCC; TGGCATGGCCGACATATGCAAGAACTATATCAGCCAGTATTCTGAAATTGCTATCCAGAT; GATGATGCACATGCAACCCAGGNAGATCTGTGCGCCTGGTTGGGTTCTGTGATGAAGTGA; AAGAAGAGCCCATGCAAA;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002778 unedited; CCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGGCTAACAGAATTTTATTGTTAAA; TCACAGAAACTTTAGTGCAAAACAAAAATCACGAAGTCCATTTAATAGCAACTTCATGTC; CTGCTGGCTTTGCTTGCTGTCTCCTGGCAACCAGAAGTGGACAGAAGCGTGGGTGCCCAA; GTGGGCCACAGACAGCTTCCAACCCCCACACCCCAGCATCCAATCCACACCCAGCAGACC; CTTCGGCATGCCGCCCTCTACCAGGAAGCCAGAGGCCTAGGAGCTCGCCATCCATATTTA; TTTGAAAAGGTCAAAAGGAGCATCTATGAGACAAGGGAGGGGTGCAGGCTGAAGCAGCGC; CTCAACAGCCAGGGACATGTAGGCAACACGAGCAGGCACAGCGCGGCCACCACTGTCCAC; ACGCTCACACAAGCCAGGCCCGCAGGGCCTTCGGAGAGCTAGCAGGTTACATTCAGGCAG; ATGGCCCTCTTCCCACCCAAACCCACAGAACCCCAAACAAGGCATCACCAGGAAAGACAC; GGGAAAGCCAAATCACAGTTGAACCAGGGACAGAGAACCCTTGCCCCACTGATGTCCCAA; GCCACCAGCAGCTGCTTCCAAATCCCTATGCTATTACAGTGGGAATTACATCATTTAAAA; AGCCTGATTATTCCAGGCTTNCTAATCTTCATATAAAACTGCCTTTGNTTTGCTGCTTTG; NTCAACTGAGAGGCCCANGAAAGCGGGGAGGGTCCCTGATCANGGCAGGAGGCCACCCTC; AGAAGCCCAGGCCCACCAGTTGCCCAGCCCATGTCAAGGCCTAGAACAAGGCCTAACCAA; GAGGTTGGATGGCCTCAGTCAGGAAACTGTGGTTATGTCAGCAGAGCTTTTCCTCCTTCA; GCAGCGCCATGCAGGGCAGCTAAAGAACTCCATGCTCACATCCATTTACAAAGAAAGGGG; CCTGACACCTTGTTTGCAGGGCTGGGGCTCCTGTGAACAAAATTAAAT;
• Restriction Sites: NotI-NotI
• ACCN: NM_002778
• Insert Size: 3110 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_002778.1, NP_002769.1
• RefSeq Size: 2767 bp
• RefSeq ORF: 1575 bp
• Locus ID: 5660
• UniProt ID: P07602
• Cytogenetics: 10q22.1
• Domains: SAPA, SAPB, SapB_1, SapB_2
• Protein Families: Druggable Genome
• Protein Pathways: Lysosome
• Summary: This gene encodes a highly conserved preproprotein that is proteolytically processed to generate four main cleavage products including saposins A, B, C, and D. Each domain of the precursor protein is approximately 80 amino acid residues long with nearly identical placement of cysteine residues and glycosylation sites. Saposins A-D localize primarily to the lysosomal compartment where they facilitate the catabolism of glycosphingolipids with short oligosaccharide groups. The precursor protein exists both as a secretory protein and as an integral membrane protein and has neurotrophic activities. Mutations in this gene have been associated with Gaucher disease and metachromatic leukodystrophy. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed. [provided by RefSeq, Feb 2016]; Transcript Variant: This variant (1) lacks an exon in the coding region, compared to variant 2, resulting in a shorter protein (isoform a), compared to isoform b.