RCN1 (NM_002901) Human Untagged Clone

SKU SC118339

RCN1 (untagged)-Human reticulocalbin 1, EF-hand calcium binding domain (RCN1)

Product Data

• Type: Human Untagged Clone
• Target Symbol: RCN1
• Synonyms: HEL-S-84; PIG20; RCAL; RCN
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118339 sequence for NM_002901 edited (data generated by NextGen Sequencing); ATGGCGCGCGGTGGCCGCGGCCGCCGCCTGGGGTTAGCCCTGGGGCTGCTGCTGGCGCTG; GTGCTGGCGCCGCGGGTTCTGCGGGCCAAGCCCACGGTGCGCAAAGAGCGCGTGGTGCGG; CCCGACTCGGAGCTGGGCGAGCGGCCCCCTGAGGACAACCAGAGCTTCCAGTACGACCAC; GAGGCCTTCCTGGGCAAGGAGGACTCCAAGACCTTCGACCAGCTCACCCCGGACGAGAGC; AAGGAGAGGCTAGGGAAGATTGTTGATCGAATCGACAATGATGGGGATGGCTTTGTCACT; ACTGAGGAGCTGAAAACCTGGATCAAACGGGTGCAGAAAAGATACATCTTTGATAATGTC; GCCAAAGTCTGGAAGGATTATGATAGGGACAAGGATGATAAAATTTCCTGGGAAGAATAC; AAACAAGCCACCTATGGTTACTACCTAGGAAACCCCGCAGAGTTTCATGATTCTTCAGAT; CATCACACCTTTAAAAAGATGCTGCCACGTGATGAGAGAAGATTCAAAGCTGCAGACCTC; AATGGTGACCTGACAGCTACTCGGGAGGAGTTCACTGCCTTTCTGCATCCTGAAGAGTTT; GAACATATGAAGGAAATTGTGGTTTTGGAAACCCTGGAGGACATCGACAAGAACGGGGAT; GGGTTTGTGGATCAGGATGAGTATATTGCGGATATGTTTTCCCATGAGGAGAATGGCCCT; GAGCCAGACTGGGTTTTATCAGAACGGGAGCAGTTTAACGAATTCCGGGATCTGAACAAG; GACGGGAAGTTAGACAAAGATGAGATTCGCCACTGGATCCTCCCTCAAGATTATGATCAT; GCACAGGCTGAGGCCAGGCATCTGGTATATGAATCAGACAAAAACAAGGATGAGAAGCTA; ACTAAAGAGGAAATATTGGAGAACTGGAACATGTTTGTCGGAAGCCAAGCTACCAATTAC; GGGGAAGATCTCACAAAAAATCATGATGAGCTTTGA; ; Clone variation with respect to NM_002901.2;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002901 unedited; GGCGGTTTTTGTATACGACTCATATAGGGCGGCCGCGAATTCGCACGAGGCGGGCACTGG; CGGAGGGACTGGCCAGTCCCCTCCTCCGCGCCGGCCCCAACCCTGTCGCTGCCGCCGCGC; TCCGAGTCCCCATTCCCGAGCTGCCGCTGTTGTCGCTCGCTCAGCGTCTCCCTCTCGGCC; GCCCTCTCCTCGGGACGATGGCGCGCGGTGGCCGCGGCCGCCGCCTGGGGTTAGCCCTGG; GGCTGCTGCTGGCGCTGGTGCTGGCGCCGCGGGTTCTGCGGGCCAAGCCCACGGTGCGCA; AAGAGCGCGTGGTGCGGCCCGACTCGGAGCTGGGCGAGCGGCCCCCTGAGGACAACCAGA; GCTTCCAGTACGACCACGAGGCCTTCCTGGGCAAGGAGGACTCCAAGACCTTCGACCAGC; TCACCCCGGACGAGAGCAAGGAGAGGCTAGGGAAGATTGTTGATCGAATCGACAATGATG; GGGATGGCTTTGTCACTACTGAGGAGCTGNAAACCTGGATCANACGGGTGCAGAANAGAT; ACATCTTTGATAATGTCGCCAAAGTCTGGGAGGATTATGATAGGGACANGGATGATAAAN; TTTCCTGGGNAGAATACANACNAGCCACCTATGGTTACTACCTANGAAACCCCGCAGAGT; TTCATGATTTCTCAGATCATCACACCTTTTAAAAGATGCTGCCACGTGATGAGAGGAAGA; TCANAGCTGCAGACCTCATGGTGACCTGACAGCTACTCGGGAGGAGTTCACTGCCTTTCT; GCATCCTGAAGAGTTTGACATATGAAGGAAATTGTGGTTTTGGAAACCCTGGNAGACATC; GANCAGACGGGGATGGGTTTGTGGATCAGGATGAGTATATTGCGGATATGTTTTTCCCAT; GAGAGAATGGCCCTGAGCCAAACTGGGTTTTATCAGAACGGGAGCAGTTTAACGT;
• Restriction Sites: NotI-NotI
• ACCN: NM_002901
• Insert Size: 2080 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_002901.1, NP_002892.1
• RefSeq Size: 2104 bp
• RefSeq ORF: 996 bp
• Locus ID: 5954
• UniProt ID: Q15293
• Cytogenetics: 11p13
• Domains: EFh
• Summary: Reticulocalbin 1 is a calcium-binding protein located in the lumen of the ER. The protein contains six conserved regions with similarity to a high affinity Ca(+2)-binding motif, the EF-hand. High conservation of amino acid residues outside of these motifs, in comparison to mouse reticulocalbin, is consistent with a possible biochemical function besides that of calcium binding. In human endothelial and prostate cancer cell lines this protein localizes to the plasma membrane.[provided by RefSeq, Jan 2009]