RGS10 (NM_002925) Human Untagged Clone

SKU SC118312

RGS10 (untagged)-Human regulator of G-protein signaling 10 (RGS10), transcript variant 2

Product Data

• Type: Human Untagged Clone
• Target Symbol: RGS10
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_002925, the custom clone sequence may differ by one or more nucleotides; ; ; ATGGAACACATCCACGACAGCGATGGCAGTTCCAGCAGCAGCCACCAGAGCCTCAAGAGCACAGCCAAAT; GGGCGGCATCCCTGGAGAATCTGCTGGAAGACCCAGAAGGCGTGAAAAGATTTAGGGAATTTTTAAAAAA; GGAATTCAGTGAAGAAAATGTTTTGTTTTGGCTAGCATGTGAAGATTTTAAGAAAATGCAAGATAAGACG; CAGATGCAGGAAAAGGCAAAGGAGATCTACATGACCTTTCTGTCCAGCAAGGCCTCATCACAGGTCAACG; TGGAGGGGCAGTCTCGGCTCAACGAGAAGATCCTGGAAGAACCGCACCCTCTGATGTTCCAGAAACTCCA; GGACCAGATCTTTAATCTCATGAAGTACGACAGCTACAGCCGCTTCTTAAAGTCTGACTTGTTTTTAAAA; CACAAGCGAACCGAGGAAGAGGAAGAAGATTTGCCTGATGCTCAAACTGCAGCTAAAAGAGCTTCCAGAA; TTTATAACACATGA; ;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002925 unedited; GGGTAGCATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGCGCGCCGTG; AGCCGGCTGAGCAGGAAGCGGCCGCCGTCAGACATCCACGACAGCGATGGCAGTTCCAGC; AGCAGCCACCAGAGCCTCAAGAGCACAGCCAAATGGGCGGCATCCCTGGAGAATCTGCTG; GAAGACCCAGAAGGCGTGAAAAGATTTAGGGAATTTTTAAAAAAGGAATTCAGTGAAGAA; AATGTTTTGTTTTGGCTAGCATGTGAAGATTTTAAGAAAATGCAAGATAAGACGCAGATG; CAGGAAAAGGCAAAGGAGATCTACATGACCTTTCTGTCCAGCAAGGCCTCATCACAGGTC; AACGTGGAGGGGCAGTCTCGGCTCAACGAGAAGATCCTGGAAGAACCGCACCCTCTGATG; TTCCAGAAACTCCAGGACCAGATCTTTAATCTCATGAAGTACGACAGCTACAGCCGCTTC; TTAAAGTCTGACTTGTTTTTAAAACACAAGCGAACCGAGGAAGAGGAAGAAGATTTGCCT; GATGCTCAAACTGCAGCTAAAAGAGCTTCCAGAATTTATAACACATGAGCCCCCAAAAAG; CCGGGACTGGCAGCTTTAAGAAGCAAAGGAATTTCCTCTCAGGACCGTGCCGGGTTTATC; ATTGCTTTGTTATTTGTAAGGACTGAAATGTACAAAACCCTTCAATGGGATGTGTGTTTT; ATTAACTGCTTCACCAGTAAATTTTGCATGATGGCTAAGCTAACATAAAAAAAAAAAAAA; AAAACTCGACTCTAGATTGCGGCCGCGGTCATAGCTGTTTCCTGAACAGATCCCCGGTGG; CATCCCTGTGACCCCTCCCCATGCCTCTCCTGGCCCTGNAAGTTGCCACTCCAGTGCCCA; CCAGCCTTGTCCTAT;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002925 unedited; GGACGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTATGTTAGCTTAGCCAT; CATGCAAAATTTACTGGTGAAGCAGTTAATAAAACACACATCCCATTGAAGGGTTTTGTA; CATTTCAGTCCTTACAAATAACAAAGCAATGATAAACCCGGCACGGTCCTGAGAGGAAAT; TCCTTTGCTTCTTAAAGCTGCCAGTCCCGGCTTTTTGGGGGCTCATGTGTTATAAATTCT; GGAAGCTCTTTTAGCTGCAGTTTGAGCATCAGGCAAATCTTCTTCCTCTTCCTCGGTTCG; CTTGTGTTTTAAAAACAAGTCAGACTTTAAGAAGCGGCTGTAGCTGTCGTACTTCATGAG; ATTAAAGATCTGGTCCTGGAGTTTCTGGAACATCAGAGGGTGCGGTTCTTCCAGGATCTT; CTCGTTGAGCCGAGACTGCCCCTCCACGTTGACCTGTGATGAGGCCTTGCTGGACAGAAA; GGTCATGTAGATCTCCTTTGCCTTTTCCTGCATCTGCGTCTTATCTTGCATTTTCTTAAA; ATCTTCACATGCTAGCCAAAACAAAACATTTTCTTCACTGAATTCCTTTTTTAAAAATTC; CCTAAATCTTTTCACGCCTTCTGGGTCTTCCAGCAGATTCTCCAGGGATGCCGCCCATTT; GGCTGTGCTCTTGAGGCTCTGGTGGCTGCTGCTGGAACTGCCATCGCTGTCGTGGATGTC; TGACGGCGGCCGCTTNCTGCTCAGCCGGCTCCCGGCGCGCCTCGTGCCGAATTCGGGCCC; GCCCTATAGTGAGTCGTATTACAAAATTCTGACGGTTCACTAAACGAGCTCTGCTTATAT; AGACCTCCCACGTACACGCCTACGGCCCATTTGCGTCACCGGGCGGNGTTATTACGACAT; TNTGGAAAGTTCCGTTGATTTTGGTGGCCAAACAAACTCCN;
• Restriction Sites: NotI-NotI
• ACCN: NM_002925
• Insert Size: 700 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_002925.3, NP_002916.1
• RefSeq Size: 859 bp
• RefSeq ORF: 504 bp
• Locus ID: 6001
• UniProt ID: O43665
• Cytogenetics: 10q26.11
• Domains: RGS
• Summary: Regulator of G protein signaling (RGS) family members are regulatory molecules that act as GTPase activating proteins (GAPs) for G alpha subunits of heterotrimeric G proteins. RGS proteins are able to deactivate G protein subunits of the Gi alpha, Go alpha and Gq alpha subtypes. They drive G proteins into their inactive GDP-bound forms. Regulator of G protein signaling 10 belongs to this family. All RGS proteins share a conserved 120-amino acid sequence termed the RGS domain. This protein associates specifically with the activated forms of the two related G-protein subunits, G-alphai3 and G-alphaz but fails to interact with the structurally and functionally distinct G-alpha subunits. Regulator of G protein signaling 10 protein is localized in the nucleus. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]; Transcript Variant: This variant (2) differs in the 5' UTR and coding region compared to variant 1. The resulting isoform (b) is shorter and has a distinct N-terminus compared to isoform a.