Sortilin (SORT1) (NM_002959) Human Untagged Clone

CAT#: SC118300

SORT1 (untagged)-Human sortilin 1 (SORT1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SORT1
• Synonyms: Gp95; LDLCQ6; NT3; NTR3
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_002959 edited
  GGCGGGCGCGCCGGGCGGCAGGTGTCGGCGTCGGCGGCATTCGGCGGCGATGGAGCGGCC
  CTGGGGAGCTGCGGACGGCCTCTCGCGCTGGCCCCATGGCCTCGGCCTCCTCCTCCTCCT
  GCAGCTGCTGCCGCCGTCGACCCTCAGCCAGGACCGGCTGGACGCGCCGCCGCCGCCCGC
  TGCGCCGCTGCCGCGCTGGTCTGGCCCCATCGGGGTGAGCTGGGGGCTGCGGGCGGCCGC
  AGCCGGGGGCGCGTTTCCCCGCGGCGGCCGTTGGCGTCGCAGCGCGCCGGGCGAGGACGA
  GGAGTGCGGCCGGGTCCGGGACTTCGTCGCCAAGCTGGCCAACAACACGCACCAGCATGT
  GTTTGATGATCTCAGAGGCTCAGTATCCTTGTCCTGGGTTGGAGATAGCACTGGGGTCAT
  TCTAGTCTTGACTACCTTCCATGTACCACTGGTAATTATGACTTTTGGACAGTCCAAGCT
  ATATCGAAGTGAGGATTATGGGAAGAACTTTAAGGATATTACAGATCTCATCAATAACAC
  CTTTATTCGGACTGAATTTGGCATGGCTATTGGTCCTGAGAACTCTGGAAAGGTGGTGTT
  AACAGCAGAGGTGTCTGGAGGAAGTCGTGGAGGAAGAATCTTCAGATCATCAGATTTTGC
  GAAGAATTTTGTGCAAACAGATCTCCCTTTTCATCCTCTCACTCAGATGATGTATAGCCC
  TCAGAATTCTGATTATCTTTTAGCTCTCAGCACTGAAAATGGCCTGTGGGTGTCCAAGAA
  TTTTGGGGGAAAATGGGAAGAAATCCACAAAGCAGTATGTTTGGCCAAATGGGGATCAGA
  CAACACCATCTTCTTTACAACCTATGCAAATGGCTCCTGCAAAGCTGACCTTGGGGCTCT
  GGAATTATGGAGAACTTCAGACTTGGGAAAAAGCTTCAAAACTATTGGTGTGAAAATCTA
  CTCATTTGGTCTTGGGGGACGTTTCCTTTTTGCCTCTGTGATGGCTGATAAGGATACCAC
  AAGAAGGATCCACGTTTCAACAGATCAAGGGGACACATGGAGCATGGCCCAGCTCCCCTC
  CGTGGGACAGGAACAGTTCTATTCTATTCTGGCAGCAAATGATGACATGGTATTCATGCA
  TGTAGATGAACCTGGAGACACTGGGTTTGGCACAATCTTTACCTCAGATGATCGAGGCAT
  TGTCTATTCCAAGTCTTTGGACCGACATCTCTACACTACCACAGGCGGAGAGACGGACTT
  TACCAACGTGACCTCCCTCCGCGGCGTCTACATAACAAGCGTGCTCTCCGAAGATAATTC
  TATCCAGACCATGATCACTTTTGACCAAGGAGGAAGGTGGACGCACCTGAGGAAGCCTGA
  AAACAGTGAATGTGATGCTACAGCAAAAAACAAGAATGAGTGCAGCCTTCATATTCATGC
  TTCCTACAGCATCTCCCAGAAACTGAATGTTCCAATGGCCCCACTCTCAGAGCCGAATGC
  CGTAGGCATTGTCATTGCTCATGGTAGCGTGGGGGATGCCATCTCAGTGATGGTTCCAGA
  TGTGTACATCTCAGATGATGGGGGTTACTCCTGGACAAAGATGCTGGAAGGACCCCACTA
  TTACACCATCCTGGATTCTGGAGGCATCATTGTGGCCATTGAGCACAGCAGCCGTCCTAT
  CAATGTGATTAAGTTCTCCACAGACGAAGGTCAATGCTGGCAAACCTACACGTTCACCAG
  GGACCCCATCTATTTCACTGGCCTAGCTTCAGAACCTGGAGCTAGGTCCATGAATATCAG
  CATTTGGGGCTTCACAGAATCTTTCCTGACCAGCCAGTGGGTCTCCTACACCATTGATTT
  TAAAGATATCCTTGAAAGGAACTGTGAAGAGAAGGACTATACCATATGGCTGGCACACTC
  CACAGACCCTGAAGATTATGAAGATGGCTGCATTTTGGGCTACAAAGAACAGTTTCTGCG
  GCTACGCAAGTCATCCGTGTGTCAGAATGGTCGAGACTATGTTGTGACCAAGCAGCCCTC
  CATCTGCCTCTGTTCCCTGGAGGACTTTCTCTGTGATTTTGGCTACTACCGTCCAGAAAA
  TGACTCCAAGTGTGTGGAACAGCCAGAACTGAAGGGCCACGACCTGGAGTTTTGTCTGTA
  CGGAAGAGAAGAACACCTAACAACAAATGGGTACCGGAAAATTCCAGGGGACAAATGCCA
  GGGTGGGGTAAATCCAGTTCGAGAAGTAAAAGACTTGAAAAAGAAATGCACAAGCAACTT
  TTTGAGTCCGGAAAAACAGAATTCCAAGTCAAATTCTGTTCCAATTATCCTGGCCATCGT
  GGGATTGATGCTGGTCACAGTCGTAGCAGGAGTGCTCATTGTGAAGAAATATGTCTGTGG
  GGGAAGGTTCCTGGTGCATCGATACTCTGTGCTGCAGCAGCATGCAGAGGCCAATGGTGT
  GGATGGTGTGGATGCTTTGGACACAGCCTCCCACACTAATAAAAGTGGTTATCATGATGA
  CTCAGATGAGGACCTCTTGGAATAGCTCTTCAGAGGAGCTGGACCCAGCATGGATGGTGG
  AACCACAGTACCTCTTACACTCCCTGTGGCTCCAACTTCAGGAAATAAATTTCCCATTGC
  GAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  
• Restriction Sites: Please inquire
• ACCN: NM_002959
• Insert Size: 2700 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: The ORF of this clone has been fully sequenced and found to be a perfect match to NM_002959.4.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002959.4, NP_002950.3
• RefSeq Size: 7018 bp
• RefSeq ORF: 2496 bp
• Locus ID: 6272
• UniProt ID: Q99523
• Cytogenetics: 1p21.3-p13.1
• Domains: BNR, VPS10
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Lysosome, Neurotrophin signaling pathway
• Gene Summary: This gene encodes a member of the VPS10-related sortilin family of proteins. The encoded preproprotein is proteolytically processed by furin to generate the mature receptor. This receptor plays a role in the trafficking of different proteins to either the cell surface, or subcellular compartments such as lysosomes and endosomes. Expression levels of this gene may influence the risk of myocardial infarction in human patients. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2015]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.