Sortilin (SORT1) (NM_002959) Human Tagged ORF Clone

CAT#: RG203429

SORT1 (tGFP-tagged) - Human sortilin 1 (SORT1), transcript variant 1

Product Images

Circular map for RG203429

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Sortilin
• Synonyms: Gp95; LDLCQ6; NT3; NTR3
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG203429 representing NM_002959
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCGGCCCTGGGGAGCTGCGGACGGCCTCTCGCGCTGGCCCCATGGCCTCGGCCTCCTCCTCCTCC
  TGCAGCTGCTGCCGCCGTCGACCCTCAGCCAGGACCGGCTGGACGCGCCGCCGCCGCCCGCTGCGCCGCT
  GCCGCGCTGGTCTGGCCCCATCGGGGTGAGCTGGGGGCTGCGGGCGGCCGCAGCCGGGGGCGCGTTTCCC
  CGCGGCGGCCGTTGGCGTCGCAGCGCGCCGGGCGAGGACGAGGAGTGCGGCCGGGTCCGGGACTTCGTCG
  CCAAGCTGGCCAACAACACGCACCAGCATGTGTTTGATGATCTCAGAGGCTCAGTATCCTTGTCCTGGGT
  TGGAGATAGCACTGGGGTCATTCTAGTCTTGACTACCTTCCATGTACCACTGGTAATTATGACTTTTGGA
  CAGTCCAAGCTATATCGAAGTGAGGATTATGGGAAGAACTTTAAGGATATTACAGATCTCATCAATAACA
  CCTTTATTCGGACTGAATTTGGCATGGCTATTGGTCCTGAGAACTCTGGAAAGGTGGTGTTAACAGCAGA
  GGTGTCTGGAGGAAGTCGTGGAGGAAGAATCTTCAGATCATCAGATTTTGCGAAGAATTTTGTGCAAACA
  GATCTCCCTTTTCATCCTCTCACTCAGATGATGTATAGCCCTCAGAATTCTGATTATCTTTTAGCTCTCA
  GCACTGAAAATGGCCTGTGGGTGTCCAAGAATTTTGGGGGAAAATGGGAAGAAATCCACAAAGCAGTATG
  TTTGGCCAAATGGGGATCAGACAACACCATCTTCTTTACAACCTATGCAAATGGCTCCTGCAAAGCTGAC
  CTTGGGGCTCTGGAATTATGGAGAACTTCAGACTTGGGAAAAAGCTTCAAAACTATTGGTGTGAAAATCT
  ACTCATTTGGTCTTGGGGGACGTTTCCTTTTTGCCTCTGTGATGGCTGATAAGGATACCACAAGAAGGAT
  CCACGTTTCAACAGATCAAGGGGACACATGGAGCATGGCCCAGCTCCCCTCCGTGGGACAGGAACAGTTC
  TATTCTATTCTGGCAGCAAATGATGACATGGTATTCATGCATGTAGATGAACCTGGAGACACTGGGTTTG
  GCACAATCTTTACCTCAGATGATCGAGGCATTGTCTATTCCAAGTCTTTGGACCGACATCTCTACACTAC
  CACAGGCGGAGAGACGGACTTTACCAACGTGACCTCCCTCCGCGGCGTCTACATAACAAGCGTGCTCTCC
  GAAGATAATTCTATCCAGACCATGATCACTTTTGACCAAGGAGGAAGGTGGACGCACCTGAGGAAGCCTG
  AAAACAGTGAATGTGATGCTACAGCAAAAAACAAGAATGAGTGCAGCCTTCATATTCATGCTTCCTACAG
  CATCTCCCAGAAACTGAATGTTCCAATGGCCCCACTCTCAGAGCCGAATGCCGTAGGCATTGTCATTGCT
  CATGGTAGCGTGGGGGATGCCATCTCAGTGATGGTTCCAGATGTGTACATCTCAGATGATGGGGGTTACT
  CCTGGACAAAGATGCTGGAAGGACCCCACTATTACACCATCCTGGATTCTGGAGGCATCATTGTGGCCAT
  TGAGCACAGCAGCCGTCCTATCAATGTGATTAAGTTCTCCACAGACGAAGGTCAATGCTGGCAAACCTAC
  ACGTTCACCAGGGACCCCATCTATTTCACTGGCCTAGCTTCAGAACCTGGAGCTAGGTCCATGAATATCA
  GCATTTGGGGCTTCACAGAATCTTTCCTGACCAGCCAGTGGGTCTCCTACACCATTGATTTTAAAGATAT
  CCTTGAAAGGAACTGTGAAGAGAAGGACTATACCATATGGCTGGCACACTCCACAGACCCTGAAGATTAT
  GAAGATGGCTGCATTTTGGGCTACAAAGAACAGTTTCTGCGGCTACGCAAGTCATCCGTGTGTCAGAATG
  GTCGAGACTATGTTGTGACCAAGCAGCCCTCCATCTGCCTCTGTTCCCTGGAGGACTTTCTCTGTGATTT
  TGGCTACTACCGTCCAGAAAATGACTCCAAGTGTGTGGAACAGCCAGAACTGAAGGGCCACGACCTGGAG
  TTTTGTCTGTACGGAAGAGAAGAACACCTAACAACAAATGGGTACCGGAAAATTCCAGGGGACAAATGCC
  AGGGTGGGGTAAATCCAGTTCGAGAAGTAAAAGACTTGAAAAAGAAATGCACAAGCAACTTTTTGAGTCC
  GGAAAAACAGAATTCCAAGTCAAATTCTGTTCCAATTATCCTGGCCATCGTGGGATTGATGCTGGTCACA
  GTCGTAGCAGGAGTGCTCATTGTGAAGAAATATGTCTGTGGGGGAAGGTTCCTGGTGCATCGATACTCTG
  TGCTGCAGCAGCATGCAGAGGCCAATGGTGTGGATGGTGTGGATGCTTTGGACACAGCCTCCCACACTAA
  TAAAAGTGGTTATCATGATGACTCAGATGAGGACCTCTTGGAA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG203429 representing NM_002959
  Red=Cloning site Green=Tags(s)
  
  MERPWGAADGLSRWPHGLGLLLLLQLLPPSTLSQDRLDAPPPPAAPLPRWSGPIGVSWGLRAAAAGGAFP
  RGGRWRRSAPGEDEECGRVRDFVAKLANNTHQHVFDDLRGSVSLSWVGDSTGVILVLTTFHVPLVIMTFG
  QSKLYRSEDYGKNFKDITDLINNTFIRTEFGMAIGPENSGKVVLTAEVSGGSRGGRIFRSSDFAKNFVQT
  DLPFHPLTQMMYSPQNSDYLLALSTENGLWVSKNFGGKWEEIHKAVCLAKWGSDNTIFFTTYANGSCKAD
  LGALELWRTSDLGKSFKTIGVKIYSFGLGGRFLFASVMADKDTTRRIHVSTDQGDTWSMAQLPSVGQEQF
  YSILAANDDMVFMHVDEPGDTGFGTIFTSDDRGIVYSKSLDRHLYTTTGGETDFTNVTSLRGVYITSVLS
  EDNSIQTMITFDQGGRWTHLRKPENSECDATAKNKNECSLHIHASYSISQKLNVPMAPLSEPNAVGIVIA
  HGSVGDAISVMVPDVYISDDGGYSWTKMLEGPHYYTILDSGGIIVAIEHSSRPINVIKFSTDEGQCWQTY
  TFTRDPIYFTGLASEPGARSMNISIWGFTESFLTSQWVSYTIDFKDILERNCEEKDYTIWLAHSTDPEDY
  EDGCILGYKEQFLRLRKSSVCQNGRDYVVTKQPSICLCSLEDFLCDFGYYRPENDSKCVEQPELKGHDLE
  FCLYGREEHLTTNGYRKIPGDKCQGGVNPVREVKDLKKKCTSNFLSPEKQNSKSNSVPIILAIVGLMLVT
  VVAGVLIVKKYVCGGRFLVHRYSVLQQHAEANGVDGVDALDTASHTNKSGYHDDSDEDLLE
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_002959
• ORF Size: 2493 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002959.4, NP_002950.3
• RefSeq Size: 7018 bp
• RefSeq ORF: 2496 bp
• Locus ID: 6272
• UniProt ID: Q99523
• Cytogenetics: 1p21.3-p13.1
• Domains: BNR, VPS10
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Lysosome, Neurotrophin signaling pathway
• Gene Summary: This gene encodes a member of the VPS10-related sortilin family of proteins. The encoded preproprotein is proteolytically processed by furin to generate the mature receptor. This receptor plays a role in the trafficking of different proteins to either the cell surface, or subcellular compartments such as lysosomes and endosomes. Expression levels of this gene may influence the risk of myocardial infarction in human patients. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2015]