STAT5A (NM_003152) Human Untagged Clone

CAT#: SC118194

STAT5A (untagged)-Human signal transducer and activator of transcription 5A (STAT5A)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: STAT5A
• Synonyms: MGF; STAT5
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_003152, the custom clone sequence may differ by one or more nucleotides
  
  
  TCGGCACGAGGCCGCCAGGAACCCCGGCCGGGAGCGAGAGCCGCGGGGCGCAGAGCCGGCCCGGCTGCCG
  GACGGTGCGGCCCCACCAGGTGAACGGCCATGGCGGGCTGGATCCAGGCCCAGCAGCTGCAGGGAGACGC
  GCTGCGCCAGATGCAGGTGCTGTACGGCCAGCACTTCCCCATCGAGGTCCGGCACTACTTGGCCCAGTGG
  ATTGAGAGCCAGCCATGGGATGCCATTGACTTGGACAATCCCCAGGACAGAGCCCAAGCCACCCAGCTCC
  TGGAGGGCCTGGTGCAGGAGCTGCAGAAGAAGGCGGAGCACCAGGTGGGGGAAGATGGGTTTTTACTGAA
  GATCAAGCTGGGGCACTACGCCACGCAGCTCCAGAAAACATATGACCGCTGCCCCCTGGAGCTGGTCCGC
  TGCATCCGGCACATTCTGTACAATGAACAGAGGCTGGTCCGAGAAGCCAACAATTGCAGCTCTCCGGCTG
  GGATCCTGGTTGACGCCATGTCCCAGAAGCACCTTCAGATCAACCAGACATTTGAGGAGCTGCGACTGGT
  CACGCAGGACACAGAGAATGAGCTGAAGAAACTGCAGCAGACTCAGGAGTACTTCATCATCCAGTACCAG
  GAGAGCCTGAGGATCCAAGCTCAGTTTGCCCAGCTGGCCCAGCTGAGCCCCCAGGAGCGTCTGAGCCGGG
  AGACGGCCCTCCAGCAGAAGCAGGTGTCTCTGGAGGCCTGGTTGCAGCGTGAGGCACAGACACTGCAGCA
  GTACCGCGTGGAGCTGGCCGAGAAGCACCAGAAGACCCTGCAGCTGCTGCGGAAGCAGCAGACCATCATC
  CTGGATGACGAGCTGATCCAGTGGAAGCGGCGGCAGCAGCTGGCCGGGAACGGCGGGCCCCCCGAGGGCA
  GCCTGGACGTGCTACAGTCCTGGTGTGAGAAGTTGGCCGAGATCATCTGGCAGAACCGGCAGCAGATCCG
  CAGGGCTGAGCACCTCTGCCAGCAGCTGCCCATCCCCGGCCCAGTGGAGGAGATGCTGGCCGAGGTCAAC
  GCCACCATCACGGACATTATCTCAGCCCTGGTGACCAGCACATTCATCATTGAGAAGCAGCCTCCTCAGG
  TCCTGAAGACCCAGACCAAGTTTGCAGCCACCGTACGCCTGCTGGTGGGCGGGAAGCTGAACGTGCACAT
  GAATCCCCCCCAGGTGAAGGCCACCATCATCAGTGAGCAGCAGGCCAAGTCTCTGCTTAAAAATGAGAAC
  ACCCGCAACGAGTGCAGTGGTGAGATCCTGAACAACTGCTGCGTGATGGAGTACCACCAAGCCACGGGCA
  CCCTCAGTGCCCACTTCAGGAACATGTCACTGAAGAGGATCAAGCGTGCTGACCGGCGGGGTGCAGAGTC
  CGTGACAGAGGAGAAGTTCACAGTCCTGTTTGAGTCTCAGTTCAGTGTTGGCAGCAATGAGCTTGTGTTC
  CAGGTGAAGACTCTGTCCCTACCTGTGGTTGTCATCGTCCACGGCAGCCAGGACCACAATGCCACGGCTA
  CTGTGCTGTGGGACAATGCCTTTGCTGAGCCGGGCAGGGTGCCATTTGCCGTGCCTGACAAAGTGCTGTG
  GCCGCAGCTGTGTGAGGCGCTCAACATGAAATTCAAGGCCGAAGTGCAGAGCAACCGGGGCCTGACCAAG
  GAGAACCTCGTGTTCCTGGCGCAGAAACTGTTCAACAACAGCAGCAGCCACCTGGAGGACTACAGTGGCC
  TGTCCGTGTCCTGGTCCCAGTTCAACAGGGAGAACTTGCCGGGCTGGAACTACACCTTCTGGCAGTGGTT
  TGACGGGGTGATGGAGGTGTTGAAGAAGCACCACAAGCCCCACTGGAATGATGGGGCCATCCTAGGTTTT
  GTGAATAAGCAACAGGCCCACGACCTGCTCATCAACAAGCCCGACGGGACCTTCTTGTTGCGCTTTAGTG
  ACTCAGAAATCGGGGGCATCACCATCGCCTGGAAGTTTGACTCCCCGGAACGCAACCTGTGGAACCTGAA
  ACCATTCACCACGCGGGATTTCTCCATCAGGTCCCTGGCTGACCGGCTGGGGGACCTGAGCTATCTCATC
  TATGTGTTTCCTGACCGCCCCAAGGATGAGGTCTTCTCCAAGTACTACACTCCTGTGCTGGCTAAAGCTG
  TTGATGGATATGTGAAACCACAGATCAAGCAAGTGGTCCCTGAGTTTGTGAATGCATCTGCAGATGCTGG
  GGGCAGCAGCGCCACGTACATGGACCAGGCCCCCTCCCCAGCTGTGTGCCCCCAGGCTCCCTATAACATG
  TACCCACAGAACCCTGACCATGTACTCGATCAGGATGGAGAATTCGACCTGGATGAGACCATGGATGTGG
  CCAGGCACGTGGAGGAACTCTTACGCCGACCAATGGACAGTCTTGACTCCCGCCTCTCGCCCCCTGCCGG
  TCTTTTCACCTCTGCCAGAGGCTCCCTCTCATGAATGTTTGAATCCCACGCTTCTCTTTGGAAACAATAT
  GCAATGTGAAGCGGTCGTGTTGTGAGTTTAGTAAGGCTGTGTACACTGACACCTTTGCAGGCATTCGGCA
  CGAGGCCGCCAGGAACCCCGGCCGGGAGCGAGAGCCGCGGGGCGCAGAGCCGGCCCGGCTGCCGGACGGT
  GCGGCCCCACCAGGTGAACGGCCATGGCGGGCTGGATCCAGGCCCAGCAGCTGCAGGGAGACGCGCTGCG
  CCAGATGCAGGTGCTGTACGGCCAGCACTTCCCCATCGAGGTCCGGCACTACTTGGCCCAGTGGATTGAG
  AGCCAGCCATGGGATGCCATTGACTTGGACAATCCCCAGGACAGAGCCCAAGCCACCCAGCTCCTGGAGG
  GCCTGGTGCAGGAGCTGCAGAAGAAGGCGGAGCACCAGGTGGGGGAAGATGGGTTTTTACTGAAGATCAA
  GCTGGGGCACTACGCCACGCAGCTCCAGAAAACATATGACCGCTGCCCCCTGGAGCTGGTCCGCTGCATC
  CGGCACATTCTGTACAATGAACAGAGGCTGGTCCGAGAAGCCAACAATTGCAGCTCTCCGGCTGGGATCC
  TGGTTGACGCCATGTCCCAGAAGCACCTTCAGATCAACCAGACATTTGAGGAGCTGCGACTGGTCACGCA
  GGACACAGAGAATGAGCTGAAGAAACTGCAGCAGACTCAGGAGTACTTCATCATCCAGTACCAGGAGAGC
  CTGAGGATCCAAGCTCAGTTTGCCCAGCTGGCCCAGCTGAGCCCCCAGGAGCGTCTGAGCCGGGAGACGG
  CCCTCCAGCAGAAGCAGGTGTCTCTGGAGGCCTGGTTGCAGCGTGAGGCACAGACACTGCAGCAGTACCG
  CGTGGAGCTGGCCGAGAAGCACCAGAAGACCCTGCAGCTGCTGCGGAAGCAGCAGACCATCATCCTGGAT
  GACGAGCTGATCCAGTGGAAGCGGCGGCAGCAGCTGGCCGGGAACGGCGGGCCCCCCGAGGGCAGCCTGG
  ACGTGCTACAGTCCTGGTGTGAGAAGTTGGCCGAGATCATCTGGCAGAACCGGCAGCAGATCCGCAGGGC
  TGAGCACCTCTGCCAGCAGCTGCCCATCCCCGGCCCAGTGGAGGAGATGCTGGCCGAGGTCAACGCCACC
  ATCACGGACATTATCTCAGCCCTGGTGACCAGCACATTCATCATTGAGAAGCAGCCTCCTCAGGTCCTGA
  AGACCCAGACCAAGTTTGCAGCCACCGTACGCCTGCTGGTGGGCGGGAAGCTGAACGTGCACATGAATCC
  CCCCCAGGTGAAGGCCACCATCATCAGTGAGCAGCAGGCCAAGTCTCTGCTTAAAAATGAGAACACCCGC
  AACGAGTGCAGTGGTGAGATCCTGAACAACTGCTGCGTGATGGAGTACCACCAAGCCACGGGCACCCTCA
  GTGCCCACTTCAGGAACATGTCACTGAAGAGGATCAAGCGTGCTGACCGGCGGGGTGCAGAGTCCGTGAC
  AGAGGAGAAGTTCACAGTCCTGTTTGAGTCTCAGTTCAGTGTTGGCAGCAATGAGCTTGTGTTCCAGGTG
  AAGACTCTGTCCCTACCTGTGGTTGTCATCGTCCACGGCAGCCAGGACCACAATGCCACGGCTACTGTGC
  TGTGGGACAATGCCTTTGCTGAGCCGGGCAGGGTGCCATTTGCCGTGCCTGACAAAGTGCTGTGGCCGCA
  GCTGTGTGAGGCGCTCAACATGAAATTCAAGGCCGAAGTGCAGAGCAACCGGGGCCTGACCAAGGAGAAC
  CTCGTGTTCCTGGCGCAGAAACTGTTCAACAACAGCAGCAGCCACCTGGAGGACTACAGTGGCCTGTCCG
  TGTCCTGGTCCCAGTTCAACAGGGAGAACTTGCCGGGCTGGAACTACACCTTCTGGCAGTGGTTTGACGG
  GGTGATGGAGGTGTTGAAGAAGCACCACAAGCCCCACTGGAATGATGGGGCCATCCTAGGTTTTGTGAAT
  AAGCAACAGGCCCACGACCTGCTCATCAACAAGCCCGACGGGACCTTCTTGTTGCGCTTTAGTGACTCAG
  AAATCGGGGGCATCACCATCGCCTGGAAGTTTGACTCCCCGGAACGCAACCTGTGGAACCTGAAACCATT
  CACCACGCGGGATTTCTCCATCAGGTCCCTGGCTGACCGGCTGGGGGACCTGAGCTATCTCATCTATGTG
  TTTCCTGACCGCCCCAAGGATGAGGTCTTCTCCAAGTACTACACTCCTGTGCTGGCTAAAGCTGTTGATG
  GATATGTGAAACCACAGATCAAGCAAGTGGTCCCTGAGTTTGTGAATGCATCTGCAGATGCTGGGGGCAG
  CAGCGCCACGTACATGGACCAGGCCCCCTCCCCAGCTGTGTGCCCCCAGGCTCCCTATAACATGTACCCA
  CAGAACCCTGACCATGTACTCGATCAGGATGGAGAATTCGACCTGGATGAGACCATGGATGTGGCCAGGC
  ACGTGGAGGAACTCTTACGCCGACCAATGGACAGTCTTGACTCCCGCCTCTCGCCCCCTGCCGGTCTTTT
  CACCTCTGCCAGAGGCTCCCTCTCATGAATGTTTGAATCCCACGCTTCTCTTTGGAAACAATATGCAATG
  TGAAGCGGTCGTGTTGTGAGTTTAGTAAGGCTGTGTACACTGACACCTTTGCAGGCAT
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003152 unedited
  CTTTCCCCCGCCCGTTGNCGCATAGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAG
  CAGAGCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCG
  AATTCGGCACGAGGCCGCCAGGAACCCCGGCCGGGAGCGAGAGCCGCGGGGCGCAGAGCC
  GGCCCGGCTGCCGGACGGTGCGGCCCCACCAGGTGAACGGCCATGGCGGGCTGGATCCAG
  GCCCAGCAGCTGCAGGGAGACGCGCTGCGCCAGATGCAGGTGCTGTACGGCCAGCACTTC
  CCCATCGAGGTCCGGCACTACTTGGCCCAGTGGATTGAGAGCCAGCCATGGGATGCCATT
  GACTTGGACAATCCCCAGGACAGAGCCCAAGCCACCCAGCTCCTGGAGGGCCTGGTGCAG
  GAGCTGCAGAAGAAGGCGGAGCACCAGGTGGGGGAAGATGGGTTTTTACTGAAGATCAAG
  CTGGGGCACTACGCCACGCAGCTCCAGAAAACATATGACCGCTGCCCCCTGGAGCTGGTC
  CGCTGCATCCGGCACATTCTGTACAATGAACAGAGGCTGGTCCGAGAAGCCAACAATTGC
  AGCTCTCCGGCTGGGATCCTGGTTGACGCCATGTCCCAGAAGCACCTTCAGATCAACCAG
  ACATTTGAGGAGCTGCGACTGGTCACGCAGGACACAGAGAATGAGCTGAAGAAACTGCAG
  CAGACTCAGGAGTACTTCATCATCCAGTACCAGGAGAGCCTGANGATCCAAGCTCAGTTT
  GCCCAGCTGGGCCAGCTGAGCCCCCAGGAGCGTCTGAGCCAGGAGACGGCCCTNCAGCAG
  AAGCAGGTGTCTCTGGNAGCCCTGGNTGCAGCGTGAGCACAGACACTGCAGCAGTACCGC
  GTGGAGCTGGCCGAGAAGCACAGAAGACCCTGCAGCTGCTGCGGAAGCAGCAGACATCAT
  CC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003152 unedited
  CGTACACTATGNACCGCGCCGCATCTATGATCGGTTTTTTTTTTTTTTTTTTATGAAAAA
  AAATTTTATTCCAAAACACTGTTTACACAAATGTGTGGTCTTTGTACAAAGTACAAAAAA
  ACCCCTCTTCTCATCCCCACCTCCCTTCAGAGAAGTTTAAACCTTGGGCTCTCAATCTTC
  CATGGTCAGCTGCCCCTGACTGCGAGTCCTACAGCCTCAGAGGGAGGAGCGTGTGGGGTT
  GGGAGCAAGGCCTCACCAGAACACAGCCAGTTACGCAACAGCGCAAGGCCTGCTCCAGGC
  CCCACCTCCAGCGGAGTCAAACCAGATCAGCTTTTACCCCTCACTCCAGATTCCACAGGC
  ACGCAGCACATGGCTGATTTGCAGCTTGTCTTTCACTGCAGTTTTTTGTTTTTGTTTTTT
  CCAGGAGGCAAGAGGACCAACCCTCCAAGTCCCGGGGCCCCTGTCCACCACCATATCCTA
  GACCCAATCTTTTCTACCTCTTTGTCGACAAGGTTACAAACAGAGAGGCAAGCAAAGAAT
  GCTGGGGCCCAACGGAGGGGAGAGAATATATATCCCGGGAAACGTGGGCAACAGCATCAT
  AGACTTGAATGAAGCCCAAGGCCAAGCAGCCAAGCAAGGACTAATTCAGAGCAGCTCAGA
  AACCCTCACTCAAACAGGCGGCCCCCCCTGTGCTAAAGACATGGGCCGGGCCCCAGACGC
  GCTCTCATAGGGTCCACAGAGAGTCTGGAGTCCACCGTCACTAAAGTATAAAAATACTAC
  TCGAAAATGAACCCCCAGCCTCCCCTTCTGAAGAGATGAAATCGGTTACTTCCAACCCCT
  TGAAGACCGAATGCGTTTTTCTTAGGACCCCCCCACTTCTTTCTTTGGTGTGTGTTG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003152
• Insert Size: 4000 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003152.2, NP_003143.2
• RefSeq Size: 4314 bp
• RefSeq ORF: 4314 bp
• Locus ID: 6776
• UniProt ID: P42229
• Cytogenetics: 17q21.2
• Domains: SH2, STAT
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Stem cell relevant signaling - DSL/Notch pathway, Stem cell relevant signaling - JAK/STAT signaling pathway, Transcription Factors
• Protein Pathways: Acute myeloid leukemia, Chronic myeloid leukemia, ErbB signaling pathway, Jak-STAT signaling pathway, Pathways in cancer
• Gene Summary: The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different growth hormones. Activation of this protein in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for tumorigenesis. The mouse counterpart of this gene is found to induce the expression of BCL2L1/BCL-X(L), which suggests the antiapoptotic function of this gene in cells. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Dec 2013]
  Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 encode the same isoform (1).