SRP14 (NM_003134) Human Untagged Clone

CAT#: SC118180

SRP14 (untagged)-Human signal recognition particle 14kDa (homologous Alu RNA binding protein) (SRP14)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SRP14
• Synonyms: ALURBP
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118180 sequence for NM_003134 edited (data generated by NextGen Sequencing)
  ATGGTGTTGTTGGAGAGCGAGCAGTTCCTGACGGAGCTGACCAGACTTTTCCAGAAGTGC
  CGGACGTCGGGCAGCGTCTATATCACCTTGAAGAAGTATGACGGTCGAACCAAACCCATT
  CCAAAGAAAGGTACTGTGGAGGGCTTTGAGCCCGCAGACAACAAGTGTCTGTTAAGAGCT
  ACCGATGGGAAGAAGAAGATCAGCACTGTGGTGAGCTCCAAGGAAGTGAATAAGTTTCAG
  ATGGCTTATTCAAACCTCCTTAGAGCTAACATGGATGGGTTGAAGAAGAGAGACAAAAAG
  AACAAAACTAAGAAGACCAAAGCAGCAGCAGCAGCAGCAGCAGCAGCACCTGCCGCAGCA
  GCAACAGCAGCAACAACAGCAGCAACAACAGCAGCAACAGCAGCACAGTAA
  
  Clone variation with respect to NM_003134.4
  129 g=>a;280 c=>t;370 c=>g
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003134 unedited
  NGGTCACCATTTGTATACGACTCCTATAGGCGGCCGCGNATTCGGCACGAGGTAGGGGCT
  TAAGCGGAGGGAGTCGAGCCAGCGTCGCCGCGATGGTGTTGTTGGAGAGCGAGCAGTTCC
  TGACGGAGCTGACCAGACTTTTCCAGAAGTGCCGGACGTCGGGCAGCGTCTATATCACCT
  TGAAGAAGTATGACGGTCGAACCAAACCCATTCCAAAGAAAGGTACTGTGGAGGGCTTTG
  AGCCCGCAGACAACAAGTGTCTGTTAAGAGCTACCGATGGGAAGAAGAAGATCAGCACTG
  TGGTGAGCTCCAAGGAAGTGAATAAGTTTCAGATGGCTTATTCAAACCTCCTTAGAGCTA
  ACATGGATGGGTTGAAGAAGAGAGACAAAAAGAACAAAACTAAGAAGACCAAAGCAGCAG
  CAGCAGCAGCAGCAGCAGCACCTGCCGCAGCAGCAACAGCAGCAACAACAGCAGCAACAA
  CAGCAGCAACAGCAGCACAGTAAAGGGCATACATTTCCTGCTTTCACCAATTAACCACTG
  AATTGCTATTTTTTCCTTTTGGCCAGATAGCTAGGTTTCTGGTTCCCCCACAGTAGGTGT
  TTTCACATAAGATTAGGGTCCTTTTGGAAAGAATAGTTGCAGTGTTTATAGGATAGTTGT
  GGTAAGAATCTAGTTTATTTTGCATTTGGCTAATTGGTCTGTGCTGCATGGTTATATACT
  CCTGGATTATAGAATANAAGTCTCCGTAGACATCTCTGTGAAGAGCCAGCTATCATTAAA
  CATGTCTGTTTATCAGCACTGTCTCTTTATTCCTTTTCCCACCCCATTTAATAGTTCTGG
  CCATAACTACTCAATCTAGAATGAGNGATTATGGAATAGCCTTAGCTCTATATATCTTCT
  AGGTATAGAAATG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003134
• Insert Size: 3670 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003134.2, NP_003125.2
• RefSeq Size: 800 bp
• RefSeq ORF: 411 bp
• Locus ID: 6727
• UniProt ID: P37108
• Cytogenetics: 15q15.1
• Domains: SRP14
• Protein Pathways: Protein export
• Gene Summary: Signal-recognition-particle assembly has a crucial role in targeting secretory proteins to the rough endoplasmic reticulum membrane. SRP9 together with SRP14 and the Alu portion of the SRP RNA, constitutes the elongation arrest domain of SRP. The complex of SRP9 and SRP14 is required for SRP RNA binding.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the shorter transcript and encodes the longer isoform (1).