CD43 (SPN) (NM_003123) Human Untagged Clone

SKU SC118170

SPN (untagged)-Human sialophorin (SPN), transcript variant 2

Product Data

• Type: Human Untagged Clone
• Target Symbol: CD43
• Synonyms: CD43; GALGP; GPL115; LSN
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118170 sequence for NM_003123 edited (data generated by NextGen Sequencing); ATGGCCACGCTTCTCCTTCTCCTTGGGGTGCTGGTGGTAAGCCCAGACGCTCTGGGGAGC; ACAACAGCAGTGCAGACACCCACCTCCGGAGAGCCTTTGGTCTCTACTAGCGAGCCCCTG; AGCTCAAAGATGTACACCACTTCAATAACAAGTGACCCTAAGGCCGACAGCACTGGGGAC; CAGACCTCAGCCCTACCTCCCTCAACTTCCATCAATGAGGGATCCCCTCTTTGGACTTCC; ATTGGTGCCAGCACTGGTTCCCCTTTACCTGAGCCAACAACCTACCAGGAAGTTTCCATC; AAGATGTCATCAGTGCCCCAGGAAACCCCTCATGCAACCAGTCATCCTGCTGTTCCCATA; ACAGCAAACTCTCTAGGATCCCACACCGTGACAGGTGGAACCATAACAACGAACTCTCCA; GAAACCTCCAGTAGGACCAGTGGAGCCCCTGTTACCACGGCAGCTAGCTCTCTGGAGACC; TCCAGAGGCACCTCTGGACCCCCTCTTACCATGGCAACTGTCTCTCTGGAGACTTCCAAA; GGCACCTCTGGACCCCCTGTTACCATGGCAACTGACTCTCTGGAGACCTCCACTGGGACC; ACTGGACCCCCTGTTACCATGACAACTGGCTCTCTGGAGCCCTCCAGCGGGGCCAGTGGA; CCCCAGGTCTCTAGCGTAAAACTATCTACAATGATGTCTCCAACGACCTCCACCAACGCA; AGCACTGTGCCCTTCCGGAACCCAGATGAGAACTCACGAGGCATGCTGCCAGTGGCTGTG; CTTGTGGCCCTGCTGGCGGTCATAGTCCTCGTGGCTCTGCTCCTGCTGTGGCGCCGGCGG; CAGAAGCGGCGGACTGGGGCCCTCGTGCTGAGCAGAGGCGGCAAGCGTAACGGGGTGGTG; GACGCCTGGGCTGGGCCAGCCCAGGTCCCTGAGGAGGGGGCCGTGACAGTGACCGTGGGA; GGGTCCGGGGGCGACAAGGGCTCTGGGTTCCCCGATGGGGAGGGGTCTAGCCGTCGGCCC; ACGCTCACCACTTTCTTTGGCAGACGGAAGTCTCGCCAGGGCTCCCTGGCGATGGAGGAG; CTGAAGTCTGGGTCAGGCCCCAGCCTCAAAGGGGAGGAGGAGCCACTGGTGGCCAGTGAG; GATGGGGCTGTGGACGCCCCAGCTCCTGATGAGCCCGAAGGGGGAGACGGGGCTGCCCCT; TAA; ; Clone variation with respect to NM_003123.3;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003123 unedited; NGGTTCGGATTTGTATACGACTCATATAGGCGGCCGCGNAATTCGCACGAGGGACCTGGC; CCCAGTGCTGCGTCCTTATCAGCCGAGCCGGTCCCAGCTCTTGCTCCTGCCTGTTTGCCT; GGAAATGGCCACGCTTCTCCTTCTCCTTGGGGTGCTGGTGGTAAGCCCAGACGCTCTGGG; GAGCACAACAGCAGTGCAGACACCCACCTCCGGAGAGCCTTTGGTCTCTACTAGCGAGCC; CCTGAGCTCAAAGATGTACACCACTTCAATAACAAGTGACCCTAAGGCCGACAGCACTGG; GGACCAGACCTCAGCCCTACCTCCCTCAACTTCCATCAATGAGGGATCCCCTCTTTGGAC; TTCCATTGGTGCCAGCACTGGTTCCCCTTTACCTGAGCCAACAACCTACCAGGAAGTTTC; CATCAAGATGTCATCAGTGCCCCAGGAAACCCCTCATGCAACCAGTCATCCTGCTGTTCC; CATAACAGCAAACTCTCTAGGATCCCACACCGTGACAGGTGGAACCATAACAACGAACTC; TCCAGAAACCTCCAGTAGGACCAGTGGAGCCCCTGTTACCACGGCAGCTAGCTCTCTGGA; GACCTCCAGAGGCACCTCTGGACCCCCTCTTACCATGGCAACTGTCTCTCTGGAGACTTC; CAAAGGCACCTCTGGACCCCCTGTTACCATGGCAACTGACTCTCTGGAGACCTCCACTGG; GACCACTGGACCCNCTGTTACCATGACAACTGGCTCTCTGGAGCCCTCCAGCGGGGCCAG; TGGACCCCAGGTCTCTAGCGTAAAACTATCTACAATGATGTCTCCAACGACCTNCACCAA; CGCAAGCACTGTGCCCTTCCCGAACCCAGATGAGAACTCACGAGGCATGCTGCCAGTGGC; TGTGCTTGTGGCCCCTGCTGCNGTCATAGTCCT;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003123 unedited; CGGCCGCAATCTAGNATCGAGTTTTTTTTTTTTTTTTTTGGAGAAAAAGGGGTGGATGAG; GAGGGTTTATTCATTCAGTCCACAGACGTTGAGAGGTGCCAGCCCTGCCCTGCCCAGGCG; CTAGTTGTGGGTGCTGGGTGAGGATGGCAGCAGGAAATCCAGGGCTGAGAAGCCTCATGA; GGATTCAGAGTGAAGAGGTGGGTGAGGAGGCAAGGCGCGGAGGCTCATGTCTGTAATCTC; AGCACTTTGGGAGGCTGAGGCAGGTAGATCACCTGAGGTCAGGAGTTTGAGACCAGCCTA; GCCAACATGGTGAAACCCCATCTCTACCAAAAATACAAAAAAGCAGCTGGGCATGGTGGT; GGGTGCCTGTAATCTCAGTTACTCGGGAAGCTGAGGCAGGAGAATCGCCTGAACTTAGGA; GGCAGAGGTTGCAGTGAACTGAGATCGTGCATTGCACTCCAGCCTGGGCGACAAAGCGAA; ACTCTGTCTCAAAAGAAAAAAAAAAAGGAGATTCGGGTGAGGATCAGGGTGCCGGGTGGA; AACATGGGAGCGAGGGGGCAGTGGGATGGTGAGAGGGAAGGTGCTGGAGGCTGGCTGAGA; TTCCGGCCTCCAGCCTCACTATTCACCGACACTTAAGGGGCAGCCCCGTCTCCCCCTTCG; GGCTCATCAGGAGCTGGGGCGTCCACAGCCCCATTCTCACTGGCCACCAGTGGCTCCTCC; TCCCCTTTGAGGCTGGGGCCTGACCCAGACTTCAGCTCCNTCATCGCCAGGGAGCCCCTG; GCGAGACTTCCGTCTGCCAAAGAAAGTGGTGAGCGTTGGCCGACGGCTAGAACCCTCCCC; ATCGGGCAACCCATAGCCCTTGTCGCCCCCGGCACCTCCCCGGTCACTTGTACGGCCCCT; TCTTAGGACCTCGGGGTTGGCCCGCCCCAGTCGTTCCCCACCCGTTACGCTTGCCCCCTT; GCCCATACCAGGCCCCA;
• Restriction Sites: NotI-NotI
• ACCN: NM_003123
• Insert Size: 1880 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_003123.2, NP_003114.1
• RefSeq Size: 2288 bp
• RefSeq ORF: 1203 bp
• Locus ID: 6693
• UniProt ID: P16150
• Cytogenetics: 16p11.2
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Transmembrane
• Protein Pathways: Cell adhesion molecules (CAMs)
• Summary: This gene encodes a highly sialylated glycoprotein that functions in antigen-specific activation of T cells, and is found on the surface of thymocytes, T lymphocytes, monocytes, granulocytes, and some B lymphocytes. It contains a mucin-like extracellular domain, a transmembrane region and a carboxy-terminal intracellular region. The extracellular domain has a high proportion of serine and threonine residues, allowing extensive O-glycosylation, and has one potential N-glycosylation site, while the carboxy-terminal region has potential phosphorylation sites that may mediate transduction of activation signals. Different glycoforms of this protein have been described. In stimulated immune cells, proteolytic cleavage of the extracellular domain occurs in some cell types, releasing a soluble extracellular fragment. Defects in expression of this gene are associated with Wiskott-Aldrich syndrome. [provided by RefSeq, Sep 2017]; Transcript Variant: This variant (2) differs in the 5' UTR, compared to variant 1. Variants 1 and 2 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.