SPARC (NM_003118) Human Untagged Clone

SKU SC118167

SPARC (untagged)-Human secreted protein, acidic, cysteine-rich (osteonectin) (SPARC)

Product Data

• Type: Human Untagged Clone
• Target Symbol: SPARC
• Synonyms: BM-40; OI17; ON; ONT
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118167 sequence for NM_003118 edited (data generated by NextGen Sequencing); ATGAGGGCCTGGATCTTCTTTCTCCTTTGCCTGGCCGGGAGGGCCTTGGCAGCCCCTNNN; NNAGAAGCCCTGCCTGATGAGACAGAGGTGGTGGAAGAAACTGTGGCAGAGGTGACTGAG; GTATCTGTGGGAGCTAATCCTGTCCAGGTGGAAGTAGGAGAATTTGATGATGGTGCAGAG; GAAACCGAAGAGGAGGTGGTGGCGGAAAATCCCTGCCAGAACCACCACTGCAAACACGGC; AAGGTGTGCGAGCTGGATGAGAACAACACCCCCATGTGCGTGTGCCAGGACCCCACCAGC; TGCCCAGCCCCCATTGGCGAGTTTGAGAAGGTGTGCAGCAATGACAACAAGACCTTCGAC; TCTTCCTGCCACTTCTTTGCCACAAAGTGCACCCTGGAGGGCACCAAGAAGGGCCACAAG; CTCCACCTGGACTACATCGGGCCTTGCAAATACATCCCCCCTTGCCTGGACTCTGAGCTG; ACCGAATTCCCCCTGCGCATGCGGGACTGGCTCAAGAACGTCCTGGTCACCCTGTATGAG; AGGGATGAGGACAACAACCTTCTGACTGAGAAGCAGAAGCTGCGGGTGAAGAAGATCCAT; GAGAATGAGAAGCGCCTGGAGGCAGGAGACCACCCCGTGGAGCTGCTGGCCCGGGACTTC; GAGAAGAACTATAACATGTACATCTTCCCTGTACACTGGCAGTTCGGCCAGCTGGACCAG; CACCCCATTGACGGGTACCTCTCCCACACCGAGCTGGCTCCACTGCGTGCTCCCCTCATC; CCCATGGAGCATTGCACCACCCGCTTTTTCGAGACCTGTGACCTGGACAATGACAAGTAC; ATCGCCCTGGATGAGTGGGCCGGCTGCTTCGGCATCAAGCAGAAGGATATCGACAAGGAT; CTTGTGATCTAA; ; Clone variation with respect to NM_003118.2; 58 c=>n;59 a=>n;60 g=>n;61 c=>n;62 a=>n
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003118 unedited; GTCGCATTTGTATACGACTCCTATAGGGCGGCCGCGAATCGGCACCAGCTCCACATTCCC; GCGGTCCTTCAGACTGCCCGGAGAGCGCGCTCTGCCTGCCGCCTGCCTGCCTGCCACTGA; GGGTTCCCAGCACCATGAGGGCCTGGATCTTCTTTCTCCTTTGCCTGGCCGGGAGGGCCT; TGGCAGCCCCTCAAGAAGCCCTGCCTGATGAGACAGAGGTGGTGGAAGAAACTGTGGCAG; AGGTGACTGAGGTATCTGTGGGAGCTAATCCTGTCCAGGTGGAAGTAGGAGAATTTGATG; ATGGTGCAGAGGAAACCGAAGAGGAGGTGGTGGCGGAAAATCCCTGCCAGAACCACCACT; GCAAACACGGCAAGGTGTGCGAGCTGGATGAGAACAACACCCCCATGTGCGTGTGCCAGG; ACCCCACCAGCTGCCCAGCCCCCATTGGCGAGTTTGAGAAGGTGTGCAGCAATGACAACA; AGACCTTCGACTCTTCCTGCCACTTCTTTGCCACAAAGTGCACCCTGGAGGGCACCAAGA; AGGGCCACAAGCTCCACCTGGACTACATCGGGCCTTGCAAATACATCCCCCCTTGCCTGG; ACTCTGAGCTGACCGAATTCCCCCTGCGCATGCGGGACTGGCTCAAGAACGTCCTGGTCA; CCCTGTATGAGAGGGATGAGGACAACAACCTTCTGACTGAGAAGCAGAAGCTGCGGGTGA; AGAAGATCCATGAGAATGAGAAGCGCCTGGNAGGCAGAGACCACCCCGTGGAGCTGCTGG; CCCCGGACTTCGAGAAGACTATAACATGTACATCCTCCCTGTACACTGGCAGTTCGGCCA; GCTGGACCAGCACCCCATTGACGGGTACCTCTCCCACACCGAGCTGGCTCCATGCGTGCT; CCCCTCATCCCATGGAGCATGCAC;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003118 unedited; CTATGGACCGCGGCACGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTTGATTTTTTA; GGAGCTTTTATTGTTTTAGTAATCTTAACATAACTTAAAATAAGAGAGGGGAAATGACAT; CTGGAGATCTAGGTATGTGGCCCATTGCAATTGAGCACATTTCTTGGGTCTGTTTCTCTA; TCTCTAAGGGGAGTCTCAAAACCCCAGCTCAAAATACGACACTAACATGATGAACATGCA; TGAGCTTTGAAAAGTGCTCTGTAGTCTTATGATGATCTAGAAGAGCACTGTCCAATAGAA; CTTTCTGTGATGATGAAAAGATTCTACTTCTGACCTATTCAATAGGGTAACCACTAATCA; TGCATGGCTCTCAAGCACTTGAAATGTTGCTAGTGTGATTGGGGAGCTGCGTTTTGAATG; TTAACTAATTTAAATTTAAATCTTAAAAAGTTACATGTGGGTAGTGGCTGCCGTACGGAA; CAGTACAGCACTAGAATAATGCTGTGTCCTCCTGTTACACATACTCATGGTCTCTCCTAC; TCTCCCTTCATCACACCTGTGACATCTTGCAATTTATATTTGATTGTGTCATTATCTAAT; GTATTCCTCCTGGACTCTAAGCTCCAAATGAACAAATGCTTTGTCTACTTTGCCATAACT; GTGTCCCCAGTGCTTGGCATGGGACCTGGTGCANAGTAAATACATCATAAATATTTTGTG; AATGAATGAATGAATGAATGACCANNTGATAATAAAAGATATAGCTGCTCANTCTGGTGC; TGATATTGGGGGANATGATCAAAAGGGTAAACCACAGGTCCTTAAATGAAAACAGCTAAC; TTATTGCTTACAAGAAACCTACCCTCCCTGGGTATAAACTGCCCGGTGTTTGTGGTCCCG; GGTGGTGTGTGCAAAAACATAATGGGGTTGCAAAGGGTGCATTTGGGGAACCATGGTAAA; CAAGATGCCCTTGGGTTTTTGGGGAAGAAAATAAAGGAACTAAAA;
• Restriction Sites: NotI-NotI
• ACCN: NM_003118
• Insert Size: 3280 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.; ; The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_003118.2, NP_003109.1
• RefSeq Size: 3178 bp
• RefSeq ORF: 912 bp
• Locus ID: 6678
• UniProt ID: P09486
• Cytogenetics: 5q33.1
• Domains: FOLN, kazal
• Protein Families: Druggable Genome
• Summary: This gene encodes a cysteine-rich acidic matrix-associated protein. The encoded protein is required for the collagen in bone to become calcified but is also involved in extracellular matrix synthesis and promotion of changes to cell shape. The gene product has been associated with tumor suppression but has also been correlated with metastasis based on changes to cell shape which can promote tumor cell invasion. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2015]; Transcript Variant: This variant (1) encodes the predominant isoform (1).