Sorbitol Dehydrogenase (SORD) (NM_003104) Human Untagged Clone

SKU SC118158

SORD (untagged)-Human sorbitol dehydrogenase (SORD), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: Sorbitol Dehydrogenase
• Synonyms: HEL-S-95n; RDH; SDH; SORD1; SORDD; XDH
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_003104, the custom clone sequence may differ by one or more nucleotides; ; ; ATGGCGGCGGCGGCCAAGCCCAACAACCTTTCCCTGGTGGTGCACGGACCGGGGGACTTGCGCCTGGAGA; ACTATCCTATCCCTGAACCAGGCCCAAATGAGGTCTTGCTGAGGATGCATTCTGTTGGAATCTGTGGCTC; AGATGTCCACTACTGGGAGTATGGTCGAATTGGGAATTTTATTGTGAAAAAGCCCATGGTGCTGGGACAT; GAAGCTTCGGGAACAGTCGAAAAAGTGGGATCATCGGTAAAGCACCTAAAACCAGGTGATCGTGTTGCCA; TCGAGCCTGGTGCTCCCCGAGAAAATGATGAATTCTGCAAGATGGGCCGATACAATCTGTCACCTTCCAT; CTTCTTCTGTGCCACGCCCCCCGATGACGGGAACCTCTGCCGGTTCTATAAGCACAATGCAGCCTTTTGT; TACAAGCTTCCTGACAATGTCACCTTTGAGGAAGGCGCCCTGATCGAGCCACTTTCTGTGGGGATCCATG; CCTGCAGGAGAGGCGGAGTTACCCTGGGACACAAGGTCCTTGTGTGTGGAGCTGGGCCAATCGGGATGGT; CACTTTGCTCGTGGCCAAAGCAATGGGAGCAGCTCAAGTAGTGGTGACTGATCTGTCTGCTACCCGATTG; TCCAAAGCCAAGGAGATTGGGGCTGATTTAGTCCTCCAGATCTCCAAGGAGAGCCCTCAGGAAATCGCCA; GGAAAGTAGAAGGTCAGCTGGGGTGCAAGCCGGAAGTCACCATCGAGTGCACGGGGGCAGAGGCCTCCAT; CCAGGCGGGCATCTACGCCACTCGCTCTGGTGGGAACCTCGTGCTTGTGGGGCTGGGCTCTGAGATGACC; ACCGTACCCCTACTGCATGCAGCCATCCGGGAGGTGGATATCAAGGGCGTGTTTCGATACTGCAACACGT; GGCCAGTGGCGATTTCGATGCTTGCGTCCAAGTCTGTGAATGTAAAACCCCTCGTCACCCATAGGTTTCC; TCTGGAGAAAGCTCTGGAGGCCTTTGAAACATTTAAAAAGGGATTGGGGTTGAAAATCATGCTCAAGTGT; GACCCCAGTGACCAGAATCCCTGA; ;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003104 unedited; GGTATATTTGTCAATACGCACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGACCAGA; GCGACCAAACGTCCCGCGCCTTCCAGGCCGCACTCCAGAGCCAAAAGAGCTCCATGGCGG; CGGCGGCCAAGCCCAACAACCTTTCCCTGGTGGTGCACGGACCGGGGGACTTGCGCCTGG; AGAACTATCCTATCCCTGAACCAGGCCCAAATGAGGTCTTGCTGAGGATGCATTCTGTTG; GAATCTGTGGCTCAGATGTCCACTACTGGGAGTATGGTCGAATTGGGAATTTTATTGTGA; AAAAGCCCATGGTGCTGGGACATGAAGCTTCGGGAACAGTCGAAAAAGTGGGATCATCGG; TAAAGCACCTAAAACCAGGTGATCGTGTTGCCATCGAGCCTGGTGCTCCCCGAGAAAATG; ATGAATTCTGCAAGATGGGCCGATACAATCTGTCACCTTCCATCTTCTTCTGTGCCACGC; CCCCCGATGACGGGAACCTCTGCCGGTTCTATAAGCACAATGCAGCCTTTTGTTACAAGC; TTCCTGACAATGTCACCTTTGAGGAAGGCGCCCTGATCGAGCCACTTTCTGTGGGGATCC; ATGCCTGCAGGAGAGGCGGAGTTACCCTGNGACACAAGGTCCTTTGTGTGTGGAGCTGGG; CCAATCGGGATGGTCACTTTGCTCGTGGCCAAAGCAATGGGAGCAGCTCAAGTAGTGGTG; ACTGATCTGTCTGCTACCCCGATTGCCAAAGCCAAGGAGAAGGGNGCTGATTTACTCCTC; CAGATCTCCAAGGAAGCCCTCAGAAAACCGCCAGGAAAGTAAAAGTCAGCTGGGGTGCCA; GCCGGAATCACCATCGAGTGCACGGGGCAGAGCCTCCATCCAGGCGGGCATTACGCCACT; CGCTCTGTGGGACCATCTTGCTTGTGGGGCTGGCTCTTAAAGACACCGAACCCCTATGAT; GCGGCCTCG;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003104 unedited; NGGTACCACCACCAATATTTATTACCCCAAGNNCAATCTGAAGAATCGGGCACAAGGGTC; ATGGCTTTCCCAGGTTACAAGAAAAATCAGAACCTCTTCAAACTCACCCTCCACCCATAG; CCAAAGTAAGGAATTAAAGGCTATAGCTCAAGCTATGTCATGGCTACTATATCCTCATTC; TTGATGAATAGTCTCAAAGTCACAAGTTGGCATGATGCGATTTAGGTCTAAATCTTACCA; GAAAAAAAACCCACCATTAAAATAAGACAAGTTCATAGAAATCATTCCAACAGAAAGATA; CCAAGAGCCTTTACTGGTAATGAGAAGAGGAAAGATTTTCTGTTTATAATAACCAGAAAG; AAATGGAATTGTCTAATAACCATCAATTGAGTATGAGCCTAGTTGAGTTGAGTATGAGTC; TAGTATGGGATGCTAATCTTCTGGGGTGGGGGAAGTCCTGTGAGCTAACCAAACCCTCCT; GGTCTCTGGTGAGGTCCACAAGTAGCAGTTTCCTGGTTGCCTGGTGACAAGGGACCATGC; TTAGGGGTGGGCTGGGGCATGTTGGGGAGGGGGTCCTTCACTTGGCAAGTGAAATAACAC; AATTCTCAGAAGCTGTAGAAGAGGCAATCTTCAAACAGTGCTGAGTAGTCCTCTTACTTT; TTCCACATTANGGGAATGCAAACTGGTTTAACCTCACATGACATTTCCTTTTTTTGTTGT; TTGTTTTTTCATTTGATTGGTTTAAAAATTATCCTGGATCCANGAAAGTGATGCCCTTTT; CTTATTCTCCTTTTATCATCAAAACANACAGNCCATATGTTCTCTAGAAACATTGCCGTT; CTAAACCGGAAGTGCTGACCAGCCTTCCATCCAGACCCTGAGTGGCAGATTTCATGAAAT; AAACCTTCGGGCCAGTTAAGTCTCTCTGCAGCTTTCTTTTCTTACTCAACCCTCATTCCC; TTTGGGGCANAGCAGGGGAACCGAAAACCCAGGGCCCATTTCCTTCCA;
• Restriction Sites: NotI-NotI
• ACCN: NM_003104
• Insert Size: 2440 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_003104.3, NP_003095.1
• RefSeq Size: 2628 bp
• RefSeq ORF: 1074 bp
• Locus ID: 6652
• UniProt ID: Q00796
• Cytogenetics: 15q21.1
• Domains: ADH_zinc_N
• Protein Families: Druggable Genome
• Protein Pathways: Fructose and mannose metabolism, Metabolic pathways
• Summary: Sorbitol dehydrogenase (SORD; EC 1.1.1.14) catalyzes the interconversion of polyols and their corresponding ketoses, and together with aldose reductase (ALDR1; MIM 103880), makes up the sorbitol pathway that is believed to play an important role in the development of diabetic complications (summarized by Carr and Markham, 1995 [PubMed 8535074]). The first reaction of the pathway (also called the polyol pathway) is the reduction of glucose to sorbitol by ALDR1 with NADPH as the cofactor. SORD then oxidizes the sorbitol to fructose using NAD(+) cofactor.[supplied by OMIM, Jul 2010]; Transcript Variant: This variant (1) represents the protein-coding transcript.