mtTFA (TFAM) (NM_003201) Human Untagged Clone

CAT#: SC118128

TFAM (untagged)-Human transcription factor A, mitochondrial (TFAM), nuclear gene encoding mitochondrial protein

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: mtTFA
• Synonyms: MTDPS15; MTTF1; MTTFA; TCF6; TCF6L1; TCF6L2; TCF6L3
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118128 sequence for NM_003201 edited (data generated by NextGen Sequencing)
  ATGGCGTTTCTCCGAAGCATGTGGGGCGTGCTGAGTGCCCTGGGAAGGTCTGGAGCAGAG
  CTGTGCACCGGCTGTGGAAGTCGACTGCGCTCCCCCTTCAGTTTTGTGTATTTACCGAGG
  TGGTTTTCATCTGTCTTGGCAAGTTGTCCAAAGAAACCTGTAAGTTCTTACCTTCGATTT
  TCTAAAGAACAACTACCCATATTTAAAGCTCAGAACCCAGATGCAAAAACTACAGAACTA
  ATTAGAAGAATTGCCCAGCGTTGGAGGGAACTTCCTGATTCAAAGAAAAAAATATATCAA
  GATGCTTATAGGGCGGAGTGGCAGGTATATAAAGAAGAGATAAGCAGATTTAAAGAACAG
  CTAACTCCAAGTCAGATTATGTCTTTGGAAAAAGAAATCATGGACAAACATTTAAAAAGG
  AAAGCTATGACAAAAAAAAAAGAGTTAACACTGCTTGGAAAACCAAAAAGACCTCGTTCA
  GCTTATAACGTTTATGTAGCTGAAAGATTCCAAGAAGCTAAGGGTGATTCACCGCAGGAA
  AAGCTGAAGACTGTAAAGGAAAACTGGAAAAATCTGTCTGACTCTGAAAAGGAATTATAT
  ATTCAGCATGCTAAAGAGGACGAAACTCGTTATCATAATGAAATGAAGTCTTGGGAAGAA
  CAAATGATTGAAGTTGGACGAAAGGATCTTCTACGTCGCACAATAAAGAAACAACGAAAA
  TATGGTGCTGAGGAGTGTTAA
  
  Clone variation with respect to NM_003201.1
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003201 unedited
  TGTAATACGACTCACTATAGGGCGGCCGCNGATTCGGCACGAGGATAACACACGCCGGAG
  GGTCGCACGCGGGTTCCAGTTGTGATTGCTGGAGTTGTGTATTGCCAGGAGGCTCTCCGA
  GATTGGGGTCGGGTCACTGCCTCATCCACCGGAGCGATGGCGTTTCTCCGAAGCATGTGG
  GGCGTGCTGAGTGCCCTGGGAAGGTCTGGAGCAGAGCTGTGCACCGGCTGTGGAAGTCGA
  CTGCGCTCCCCCTTCAGTTTTGTGTATTTACCGAGGTGGTTTTCATCTGTCTTGGCAAGT
  TGTCCAAAGAAACCTGTAAGTTCTTACCTTCGATTTTCTAAAGAACAACTACCCATATTT
  AAAGCTCAGAACCCAGATGCAAAAACTACAGAACTAATTAGAAGAATTGCCCAGCGTTGG
  AGGGAACTTCCTGATTCAAAGAAAAAAATATATCAAGATGCTTATAGGGCGGAGTGGCAG
  GTATATAAAGAAGAGATAAGCAGATTTAAAGAACAGCTAACTCCAAGTCAGATTATGTCT
  TTGGAAAAAGAAATCATGGACAAACATTTAAAAAGGAAAGCTATGACAAAAAAAAAAGAG
  TTAACACTGCTTGGAAAAACCAAAAAGACCTCGTTCAGCTTATAACGTTTATGTAGCTGA
  AAGATTCCAAGAAGCTAAGGGTGATTCACCGNCAGAAAAAGCTGAGACTGGTAAGCANAA
  CTGCGANAATCTGTCTGACTCTGAANAGGAAATATATATTCAGCATGCTAAAGAAAACGA
  AACTCGTTATCATAATGAAATGAAGTCTCGCGAAGAACAAATGATTGNAAGTGGACGAAA
  GGATCCTCTACGTCGCACAATAAAGGAACAACGAAATAATGTGCTGAGGAGTGTTAAAGT
  ANAAGATGANNAGGGCTCCCATGGNATAGCCCAGNAACCCT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003201 unedited
  TGGAACGCAGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGGTTGTGTTTCAA
  CAAACTTTATTTATGAACACTAAATATTCATGTTAATTTTCAAGTATCATAAAAGTCTTT
  AAAAAAAAACATTTAAAATTATAAACACAATTCCTAGCTCACAGGCCATAAAAAGCAGGC
  AGCAGGCTGGATTTGATCCACAGGCCATAGTTGGCTGTCTCTTAAACAGGCTTTTATACG
  TTATGCAAAGTGTTTCAAATTCCAGAGACTTTGGAGACTTTTCGTTTTTTAGATTTCTCA
  TTTTAAAACACACATAATACAAGTTTTCTTTCTGGATCAATATACTCACGAGAGAAAATA
  ATTCAGAAAAAAATAAAATTCCTTACTTAAAAAAAGGGAAAAGCTATAGAACAAATGTTT
  CGTCTTAAAATATATTTAGGAAATGTAAAATTAAAGATTAAAAATTTTATTCTATGCAAC
  ATAAAATAATATGCATAAATCTGGCACATAATTTCTTTGTTTTTACTTTAGAAAAAAGAC
  AGTAAGAAAAAAGTTTATAAATTTCTCAGTCCCTTTTATACAAAGTTGCAACTAAAACCA
  GCAATCACTTATATGTACAACCATCAACTCTGAATACAATGTGAATTATACCTAGGAACT
  GTAATTTACCTAGATGAGTTCTGCCTGCTTTATGAAAAACACACACTTATATCACAGAAC
  ACCGTGGCTTCTACAATTTGTAACAATTTTGAAAACGAAACTCCTGCCTCCATAATATAA
  GGAAACAAGAGTACTCTTTCCATAGTCATCATCCTGTATAAAAAAAACTTCATAGTCTGA
  TTTACCTTTGNCAAAGGTANAATGATTCACATTTGCTTGGGATACTCTGATCCTCAGAGG
  TCCTAAACACAAATAAAGTTTGCATGC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003201
• Insert Size: 1920 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003201.1, NP_003192.1
• RefSeq Size: 1936 bp
• RefSeq ORF: 741 bp
• Locus ID: 7019
• UniProt ID: Q00059
• Cytogenetics: 10q21.1
• Domains: HMG
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Huntington's disease
• Gene Summary: This gene encodes a key mitochondrial transcription factor containing two high mobility group motifs. The encoded protein also functions in mitochondrial DNA replication and repair. Sequence polymorphisms in this gene are associated with Alzheimer's and Parkinson's diseases. There are pseudogenes for this gene on chromosomes 6, 7, and 11. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012]
  Transcript Variant: This variant (1, also known as ref) encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.