SM22 alpha (TAGLN) (NM_003186) Human Untagged Clone

CAT#: SC118118

TAGLN (untagged)-Human transgelin (TAGLN), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SM22 alpha
• Synonyms: SM22; SM22-alpha; SMCC; TAGLN1; WS3-10
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118118 sequence for NM_003186 edited (data generated by NextGen Sequencing)
  ATGGCCAACAAGGGTCCTTCCTATGGCATGAGCCGCGAAGTGCAGTCCAAAATCGAGAAG
  AAGTATGACGAGGAGCTGGAGGAGCGGCTGGKGGAGTGGATCATAGTGCAGTGTGGCCCT
  GATGTGGGCCGCCCAGACCGTGGGCGCTTGGGCTTCCAGGTCTGGCTGAAGAATGGCGTG
  ATTCTGAGCAAGCTGGTGAACAGCCTGTACCCTGATGGCTCCAAGCCGGTGAAGGTGCCC
  GAGAACCCACCCTCCATGGTCTTCAAGCAGATGGAGCAGGTGGCTCAGTTCCTGAAGGCG
  GCTGAGGACTATGGGGTCATCAAGACTGACATGTTCCAGACTGTTGACCTCTTTGAAGGC
  AAAGACATGGCAGCAGTGCAGAGGACCCTGATGGCTTTGGGCAGCTTGGCAGTGACCAAG
  AATGATGGGCACTACCGTGGAGATCCCAACTGGTTTATGAAGAAAGCGCAGGAGCATAAG
  AGGGAATTCACAGAGAGCCAGCTGCAGGAGGGAAAGCATGTCATTGGCCTTCAGATGGGC
  AGCAACAGAGGGGCCTCCCAGGCCGGCATGACAGGCTACGGACGACCTCGGCAGATCATC
  AGTTAG
  
  Clone variation with respect to NM_003186.3
  92 t=>k
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003186 unedited
  TAGGGCGGACAAGCCATTCGGCACGAGGCACAAGTCTTCACTCCTTCCTGCGAGCCCTGA
  GGAAGCCTTCTTTCCCCAGACATGGCCAACAAGGGTCCTTCCTATGGCATGAGCCGCGAA
  GTGCAGTCCAAAATCGAGAAGAAGTATGACGAGGAGCTGGAGGAGCGGCTGGTGGAGTGG
  ATCATATTGTTGTGTGGCCCTGATGTGGGCCGCCCAGACCGTGGGCGCTTGGGCTTCCAG
  GTCTGGCTGAAGAATGGCGTGATTCTGAGCAAGCTGGTGAACAGCCTGTACCCTGATGGC
  TCCAAGCCGGTGAAGGTGCCCGAGAACCCACCCTCCATGGTCTTCAAGCAGATGGAGCAG
  GTGGCTCAGTTCCTGAAGGCGGCTGAGGACTATGGGGTCATCAAGACTGACATGTTCCAG
  ACTGTTGACCTCTTTGAAGGCAAAGACATGGCAGCAGTGCAGAGGACCCTGATGGCTTTG
  GGCAGCTTGGCAGTGACCAAGAATGATGGGCACTACCGTGGAGATCCCAACTGGTTTATG
  AAGAAAGCGCAGGAGCATAAGAGGGAATTCACAGAGAGCCAGCTGCAGGAGGGAAAGCAT
  GTCATTGGCCTTCAGATGGGCAGCAACAGAGGGGCCTCCCAGGCCGGCATGACAGGCTAC
  GGACGACCTCGGCAGATCATCAGTTAGAGCGGAGAGGGGCTAGCCTGAGCCCGGCCCTCC
  CCCAGCTCCTTGGCTGCAGCCATCCCGCTTAGCCTGCCTCACCCACACCCGTGTGGTACC
  TTCAGCCCTGGCCAAGNNCTTGAGCTCTGTCACTGAGCAATGGTAACTGACCCTGGGCAG
  CTCCTCCCTGTGCCCCCAGNCTCAGCCCAACTTCTTACCCGAAGCATCACA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003186 unedited
  GGCCGCAATCTANAGTCGAGTTTTTTTTTTTTTTTTTTGAAAAAAGATGATTTTTTTTTT
  TTTTTTTTGAGTTCCAACCCCAAAAATATTCCAGGACAAGATAGGGGGCAGGCTGGGCTG
  GTTCTTCTTCAATGGGCTTTTGCCGCCAAGGAGGACAGTGGACTTGGCCCACATCCAGGC
  TACCCCCAGCCCGCTTCTCCCCTGCTTAACCCAGGGAGGAGACAGTAAAGGTGATGGGGG
  CAGCCGGGAGGGAGGGGCCAAGGCAGTGATGCTTTCGGGTAAAAAGTTGGGCTGAGGCTG
  GGGGCACAGGGAGGAGCTGCCCAGGTGCAGTTACCATTGCTCAGTGACAGAGCCTCAAAG
  CTTGGCCAGGGCTGAAGGTACCACACGGGTGTGGGTGAGGCAGGCTAAACGGGATGGCTG
  CAGCCAAGGAGCTGGGGGAGGGCCGGGCTCAAGGCTAGCCCTCTCCGCTCTAACTGATGA
  TCTGCCGAGGTCGTCCGTAGCCTGTCATGCCGGCCTGGGAGGCCCCTCTGTTGCTGCCCA
  TCTGAAGGCCAATGACATGCTTTCCCTCCTGCAGCTGGCTCTCTGTGAATTCCCTCTTAT
  GCTCCTGCGCTTTCTTCATAAACCAGTTGGGATCTCCACGGTAGTGCCCATCATTCTTGG
  TCACCTGCCAGCTGGCCAAAGCCATCAGGGTCCTCTGCACTGCTGCCATGTCTTTGCCTT
  CAAAAGGTCAACAGTTTGGAACATGGCAGTCTTGATGACCCCATATTCCTCAACCCGCCT
  TCAGGAACTGAGCCCCCCGGCTCCAACTGCCTGGAGAACATGGGAGGGTGGGTTCCTTGG
  GCACCTATCCGGGCTTGAGACCATAAGGGGTACAGCGTGGTCACCCAAGTTGGGCTAAAA
  ATTACGCCCACTCTCTCAACCCCGAACCTGTGAAGCCCAATGCCCCCACGGGTTTGGGCC
  GCGCCCACTAAGGGGCACACCGGGCTTTGGATTCCTTCACACACCCCGCTTTCACGTGTC
  CTCTGCTAAA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003186
• Insert Size: 1050 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003186.3, NP_003177.2
• RefSeq Size: 1177 bp
• RefSeq ORF: 606 bp
• Locus ID: 6876
• UniProt ID: Q01995
• Cytogenetics: 11q23.3
• Domains: calponin, CH
• Gene Summary: This gene encodes a shape change and transformation sensitive actin-binding protein which belongs to the calponin family. It is ubiquitously expressed in vascular and visceral smooth muscle, and is an early marker of smooth muscle differentiation. The encoded protein is thought to be involved in calcium-independent smooth muscle contraction. It acts as a tumor suppressor, and the loss of its expression is an early event in cell transformation and the development of some tumors, coinciding with cellular plasticity. The encoded protein has a domain architecture consisting of an N-terminal calponin homology (CH) domain and a C-terminal calponin-like (CLIK) domain. Mice with a knockout of the orthologous gene are viable and fertile but their vascular smooth muscle cells exhibit alterations in the distribution of the actin filament and changes in cytoskeletal organization. [provided by RefSeq, Aug 2017]
  Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 both encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.