Thimet Oligopeptidase (THOP1) (NM_003249) Human Untagged Clone

SKU SC118078

THOP1 (untagged)-Human thimet oligopeptidase 1 (THOP1)

Product Data

• Type: Human Untagged Clone
• Target Symbol: Thimet Oligopeptidase
• Synonyms: EP24.15; MEPD_HUMAN; MP78; TOP
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118078 sequence for NM_003249 edited (data generated by NextGen Sequencing); ATGAAGCCCCCCGCAGCCTGTGCAGGAGACATGGCGGACGCAGCATCTCCGTGCTCTGTG; GTAAACGACCTGCGGTGGGACCTGAGTGCCCAGCAGATAGAGGAGCGCACCAGGGAGCTC; ATCGAGCAGACCAAGCGCGTGTATGACCAGGTTGGCACCCAGGAGTTTGAGGACGTGTCC; TACGAGAGCACGCTCAAGGCGCTGGCCGATGTGGAGGTCACCTACACAGTTCAGAGGAAT; ATCCTTGACTTCCCCCAGCATGTTTCCCCCTCCAAGGACATCCGGACAGCCAGCACAGAG; GCCGACAAGAAGCTCTCTGAGTTCGACGTGGAGATGAGCATGAGGGAGGACGTGTACCAG; AGGATCGTGTGGCTCCAGGAGAAAGTTCAGAAGGACTCACTGAGGCCCGAGGCTGCGCGG; TACCTGGAGCGGCTAATCAAGCTGGGCCGGAGAAATGGGCTTCACCTCCCCAGAGAGACT; CAGGAAAACATCAAACGCATCAAGAAGAAGCTGAGCCTTCTGTGCATCGACTTCAACAAG; AACCTGAACGAGGACACGACCTTCCTGCCCTTCACGCTCCAGGAGCTAGGAGGGCTCCCC; GAGGACTTTCTGAACTCCCTGGAGAAGATGGAGGACGGCAAGTTGAAGGTCACCCTCAAG; TACCCCCATTACTTCCCCCTCCTGAAGAAATGCCACGTGCCTGAGACCAGGAGGAAAGTG; GAGGAGGCCTTCAACTGCCGGTGCAAGGAGGAGAACTGCGCTATCCTCAAGGAGCTGGTG; ACGCTGCGGGCCCAGAAGTCCCGCCTGCTGGGGTTCCACACGCACGCCGACTATGTCCTG; GAGATGAACATGGCCAAGACCAGCCAGACCGTGGCCACCTTCCTAGATGAGCTGGCGCAG; AAGCTGAAGCCCCTGGGGGAGCAGGAGCGTGCGGTGATTCTGGAGCTGAAGCGTGCGGAG; TGCGAGCGCCGGGGCCTGCCCTTCGACGGCCGCATCCGTGCCTGGGACATGCGCTACTAC; ATGAACCAGGTGGAGGAGACGCGCTACTGCGTGGACCAGAACCTGCTCAAGGAGTACTTC; CCCGTGCAGGTGGTCACGCACGGGCTGCTGGGCATCTACCAGGAGCTCCTGGGGCTGGCC; TTCCACCACGAGGAGGGCGCCAGTGCCTGGCATGAGGACGTGCGGCTCTACACCGCGAGG; GACGCGGCCTCGGGGGAGGTGGTCGGCAAGTTCTACCTGGACCTGTACCCGCGGGAAGGA; AAGTACGGGCACGCGGCCTGCTTTGGCCTGCAGCCCGGCTGCCTGCGGCAGGATGGGAGC; CGCCAGATCGCCATCGCGGCCATGGTGGCCAACTTCACCAAGCCCACAGCCGACGCGCCC; TCGCTGCTGCAGCATGACGAGGTGGAGACCTACTTCCATGAGTTTGGCCACGTGATGCAC; CAGCTCTGCTCCCAGGCGGAGTTCGCCATGTTCAGCGGGACCCACGTGGAGCGGGACTTT; GTGGAGGCGCCGTCGCAGATGCTGGAGAACTGGGTGTGGGAGCAGGAGCCGCTGCTGCGG; ATGTCGCGGCACTACCGCACAGGCAGCGCCGTGCCCCGGGAGCTCCTGGAGAAGCTCATT; GAGTCCCGGCAGGCCAACACAGGCCTCTTCAACCTGCGCCAGATCGTCCTCGCCAAGGTG; GACCAGGCCCTGCACACGCAGACGGACGCAGACCCCGCCGAGGAGTATGCGCGGCTCTGC; CAGGAGATCCTCGGGGTCCCGGCCACGCCAGGAACCAACATGCCTGCAACCTTCGGCCAT; CTGGCAGGTGGCTACGACGCCCAGTACTACGGGTACCTGTGGAGCGAGGTGTATTCCATG; GACATGTTCCACACGCGCTTCAAGCAGGAGGGTGTCCTGAACAGCAAGGTTGGCATGGAT; TACAGAAGCTGCATCCTGAGACCCGGCGGTTCCGAGGATGCCAGCGCCATGCTGAGGCGC; TTCCTGGGCCGTGACCCCAAGCAGGACGCCTTCCTCCTGAGCAAGGGGCTGCAGGTCGGG; GGCTGCGAGCCCGAGCCGCAGGTCTGCTGA; ; Clone variation with respect to NM_003249.3;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003249 unedited; ATTTTGTAATACGACTTACTATAGGGCGGCCGCGCAATTCGCACGAGGGGCGGCGGGCCC; CTTGGTCCTCAGGCGGCCGTGGCGGCGGTGGCGGCGGTTGGGCCGAGGCAGGCGGCCTCA; GTGGCCGAGGTGGCTGGACGCGTAGCAGGTGGAAGGAGGGAGGGAGCCGCAGGCGCAGAC; CCACCCGCCATGAAGCCCCCCGCAGCCTGTGCAGGAGACATGGCGGACGCAGCATCTCCG; TGCTCTGTGGTAAACGACCTGCGGTGGGACCTGAGTGCCCAGCAGATAGAGGAGCGCACC; AGGGAGCTCATCGAGCAGACCAAGCGCGTGTATGACCAGGTTGGCACCCAGGAGTTTGAG; GACGTGTCCTACGAGAGCACGCTCAAGGCGCTGGCCGATGTGGAGGTCACCTACACAGTT; CAGAGGAATATCCTTGACTTCCCCCAGCATGTTTCCCCCTCCAAGGACATCCGGACAGCC; AGCACAGAGGCCGACAAGAAGCTCTCTGAGTTCGACGTGGAGATGAGCATGAGGGAGGAC; GTGTACCAGAGGATCGTGTGGCTCCAGGAGAAAGTTCAGAAGGACTCACTGAGGCCCGAG; GCTGCGCGGTACCTGGAGCGGCTAATCAAGCTGGGCCGGAGAAATGGGCTTCACCTCCCC; AGAGAGACTCAGGANAACATCAAACGCATCAAGAAGAAGCTGAGCCTTCTGTGCATCGAC; TTCAACAAGAACCTGAACGAGGACACGACCTTCCTGCCCTTCACGCTCCAGGAGCTAGGA; GGGCTCCCCGAGGACTTTCTGAAACTCCTGGAGAAGATGGNAGACNGGCAGNTGAAGGTC; ACCCNTTCAGTACCCCATTACTTTCCCCTNNCTGAGAAATGCCACGTGCCTGAGACCAGN; NNAGAAGTGGNAGAGGCCCTTCACTGNCCNGTGCAGGGAGAGACTGCGCTTNCTCAGGAG; CTGTGACGCTCNGNCCAAAGTCCGCTGCTGGGT;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003249 unedited; CGCGGCCGCAATCTAGNATCGAGTTTTTTTTTTTTTTTTTTCTGTTTCCTTTTAATGACC; TCATTTCAAAAAAAAGCCGTCTCCTGACAAGGGACGTTTCCCAGAGAGGAGACGTGTTAG; TGCAACAAAGACCAGGCCCTGGCAGCCACGAAAGCCCTCCAGATGCCTTGAGGACGCCGT; CTCTAGCCGGGTGGGCCACGACCGGGTGGGGACAGACAATGACAAGAGGCAAGACAGCCG; CTCAGCCACCCTGCCAGTCCCAGGCACTGTGCCAGAGCCTGCCCCATCCTGTGCCGGGGG; CTAAGGCACCAGGGCGGCGGGAGCGCAGGCCAGACTGGGCAGTCGCAGTGCCAGGCCTCA; GCAGACCTGCGGCTCGGGCTCGCAGCCCCCGACCTGCAGCCCCTTGCTCAGGAGGAAGGC; GTCCTGCTTGGGGTCACCGGCCCAGGAAGCGCCTCAGCATGGCGCTGGCATCCTCGGAAC; CGCCGGGTCTCAGGATGCAGCTTCTGTAATCCATGCCAACCTTGCTGTTCAGGACACCCT; CCTGCTTGAAGCGCGTGTGGAACATGTNCATGGAATACACCTCGCTCCACAGGTACCCGT; AGCACTGCGCGTCGTACCCACCTGCCAGATTGGCCGAAGCTGCAGGCATGCTGGTTCCTG; GCGTGCCCGGGACCCNGAGGATCTCCTGCCAGAGCCGCGCATACTCCTCGGTGGGGTCTG; CGCCCGTTTGCGTGTGCAAGGCCTGATCCACCTGGCCGAGGACAACCTGGCGCACGTTGA; AAAAGCCTGTTGTTGCCCGCCCGGACTCATTGAGCTTCTCCAGAACCTACCGGGGCAAGG; TCCTGCCTGGGCGGTAATGCCGCAACTTCCGCATCAAGGGTTCCTTGGTCCCAACCCCAG; TTTCTCACAATTGTGGGAAGCGGCCTCACACAAAG;
• Restriction Sites: NotI-NotI
• ACCN: NM_003249
• Insert Size: 2680 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_003249.3, NP_003240.1
• RefSeq Size: 2568 bp
• RefSeq ORF: 2070 bp
• Locus ID: 7064
• UniProt ID: P52888
• Cytogenetics: 19p13.3
• Domains: Peptidase_M3
• Protein Families: Druggable Genome, Protease
• Protein Pathways: Renin-angiotensin system
• Summary: The protein encoded by this gene is a kininase that uses zinc as a cofactor. The encoded oligopeptidase cleaves cytosolic peptides, making them unavailable for display on antigen-presenting cells. This protein also cleaves neuropeptides under 20 aa in length and can degrade beta-amyloid precursor protein to amyloidogenic peptides. [provided by RefSeq, Nov 2015]