STAM1 (STAM) (NM_003473) Human Untagged Clone

CAT#: SC117957

STAM (untagged)-Human signal transducing adaptor molecule (SH3 domain and ITAM motif) 1 (STAM), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: STAM1
• Synonyms: STAM-1; STAM1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC117957 sequence for NM_003473 edited (data generated by NextGen Sequencing)
  ATGCCTCTTTTTGCCACCAATCCCTTCGATCAGGATGTTGAGAAAGCAACCAGCGAGATG
  AATACTGCTGAGGACTGGGGCCTCATTTTGGATATCTGTGATAAAGTTGGTCAGTCTCGC
  ACTGGACCTAAGGATTGTCTTCGGTCTATTATGAGAAGAGTGAACCACAAAGATCCTCAC
  GTTGCTATGCAGGCTTTGACTCTTCTAGGAGCATGTGTATCAAACTGTGGCAAAATTTTT
  CATTTAGAAGTATGTTCAAGAGATTTTGCTAGTGAAGTAAGCAACGTATTAAATAAGGGT
  CATCCTAAAGTATGTGAAAAATTAAAGGCTCTTATGGTTGAATGGACAGATGAATTTAAG
  AATGATCCACAGCTTAGTCTAATATCAGCAATGATTAAGAACCTTAAGGAACAAGGAGTT
  ACGTTCCCAGCTATTGGCTCTCAGGCTGCAGAACAAGCAAAAGCAAGCCCAGCTCTTGTA
  GCCAAGGATCCTGGTACTGTGGCTAACAAAAAAGAAGAAGAAGATTTAGCAAAAGCCATT
  GAGTTGTCTCTCAAGGAACAAAGGCAGCAGTCAACCACCCTTTCCACTTTGTATCCAAGC
  ACATCCAGTCTCTTAACTAACCACCAACATGAAGGCCGAAAAGTTCGTGCTATATATGAC
  TTTGAAGCTGCTGAAGACAATGAACTTACTTTTAAAGCTGGAGAAATTATTACAGTTCTT
  GATGACAGTGATCCTAACTGGTGGAAAGGTGAAACCCATCAAGGCATAGGGTTATTTCCT
  TCTAATTTTGTGACTGCAGATCTCACTGCTGAACCAGAAATGATTAAAACAGAGAAGAAG
  ACGGTACAATTTAGTGATGATGTTCAGGTAGAGACAATAGAACCAGAGCCGGAACCAGCC
  TTTATTGATGAAGATAAAATGGACCAGTTGCTACAGATGCTGCAAAGTACAGACCCCAGT
  GATGATCAGCCAGACCTACCAGAGCTGCTTCATCTTGAAGCAATGTGTCACCAGATGGGA
  CCTCTCATTGATGAAAAGCTGGAAGATATTGATAGAAAACATTCAGAACTCTCAGAACTT
  AATGTGAAAGTGATGGAGGCCCTTTCCTTATATACCAAGTTAATGAACGAAGATCCGATG
  TATTCCATGTATGCAAAGTTACAGAATCAGCCATATTATATGCAGTCATCTGGTGTTTCT
  GGTTCTCAGGTGTATGCAGGGCCTCCTCCAAGTGGTGCCTACCTGGTTGCAGGGAACGCG
  CAGATGAGCCACCTCCAGAGCTACAGTCTTCCCCCGGAGCAGCTGTCTTCTCTCAGCCAG
  GCAGTGGTCCCACCATCCGCAAACCCAGCCCTTCCTAGTCAGCAGACTCAGGCCGCTTAC
  CCAAATACAATGGTCAGTTCCGTTCAAGGAAACACATATCCCAGCCAGGCGCCAGTATAT
  AGTCCTCCTCCTGCCGCTACTGCTGCTGCTGCAACTGCCGATGTCACTCTGTACCAGAAT
  GCAGGACCTAATATGCCCCAGGTGCCAAACTATAACTTAACATCATCAACTCTGCCTCAG
  CCCGGAGGCAGCCAACAGCCACCTCAGCCACAGCAACCATATTCTCAGAAGGCTCTGCTA
  TAG
  
  Clone variation with respect to NM_003473.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003473 unedited
  TTCAGAATTTTGTAATACGAACTCACTATAGGGCGGCCGCGATTCGGCACGAGGGTCGCG
  GACCCTGTAGAGTCGGTCTCTGTTGCTCTTTTTGCCTGAGGAGTCTTCCATCCTACGTCG
  AGCTCTGACTCCCGTGCTGTCGAGAGGGAGTCCCCGGGGACACCTCGGCACGCAGCGGAG
  ATGCCTCTTTTTGCCACCAATCCCTTCGATCAGGATGTTGAGAAAGCAACCAGCGAGATG
  AATACTGCTGAGGACTGGGGCCTCATTTTGGATATCTGTGATAAAGTTGGTCAGTCTCGC
  ACTGGACCTAAGGATTGTCTTCGGTCTATTATGAGAAGAGTGAACCACAAAGATCCTCAC
  GTTGCTATGCAGGCTTTGACTCTTCTAGGAGCATGTGTATCAAACTGTGGCAAAATTTTT
  CATTTAGAAGTATGTTCAAGAGATTTTGCTAGTGAAGTAAGCAACGTATTAAATAAGGGT
  CATCCTAAAGTATGTGAAAAATTAAAGGCTCTTATGGTTGAATGGACAGATGAATTTAAG
  AATGATCCACAGCTTAGTCTAATATCAGCAATGATTAAGAACCTTAAGGAACAAGGAGTT
  ACGTTCCCAGCTATTGGCTCTCAGGCTGCAGAACAAGCAAAAGCAAGCCCAGCTCTTGTA
  GCCAAGGATCCTGGTACTGTGGCTAACANAANAGAAGAAGAAGATTTAGCANCAGCCATT
  TGAGTGTCTCTCAAGGACANAGGCAGCAGTCAACCACCCTTTCCACTTTGTATCCAAGCA
  CATCCAGTCTCTTAACTAACCACCACCATGAAGCCGAAAGTTCGTGCTATATATGACTTT
  GAAGCTGCTGAAGACAATGAAACTACTTTTAAAGCTGGAGAAATATTACAGNTCTTGATG
  ACAGGGATCCCTACTGGTGGAANAGGGAAAACCCATCG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003473 unedited
  CCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTCTACTTATTTCCATTTTAATGAAG
  AATTAAAGGATACAATGTGTTAAAGACATATTTAAAATACTAGCAAGGGATTAGACAGAC
  GAATCAAATTTTGTTGATATCCCAAATAATTACAAGAGACTTCGAAAATGTAGTGTAATT
  CAGGTTTTCTTTCCAGTTTAAAAATTTCTATCCATTGCCTCTATCTTTGGTGTCACTGCC
  ACCAATAAACACAGTATACAGCTTAGAAACCTAATTACTATCTTCAACTAGGAAAAGGTA
  AACCAACATCATTTCTTTAAAATGAGAAATAAAGAATGTGATCGTACTTAATTTTGTCTC
  ATGGTCCCACAATACTCTGAAATGTCATGCCAAAATGTAAAAGTTCAAAAGGGAACATTA
  TCATTTGCTATAATTGCACCAAAAATTTAGCTACTGTACGCTGGTGTGATGACAGCCAGT
  AGTATGCAGAGGCTACAGAAAGACATGTAGATATATATATATACACACACATATATATAT
  ATATCTCTTTAGCTCACAATTAGTAAAAGGTTCAGTTTAATGTGAAACTGAAACACACGA
  CAACATAATGCGCCTTCATGTAAATACGCTAACAACTTTGTTTTCTTGAAGTTTAAAAGG
  ATAGTATAATTACCTTAAATTTACACAAAACATTAAGAAACATGGGNAGTCTGTTTATGA
  TTAAAATAAAATAGCAACGTAAATTAATTTAACACAGTGTTAAATATATTCACACGATCA
  CCACTGTGATTCANAATGGCCACTATGCAGTTAATATCATATATGTCCATACATTTGCAC
  ACAGTTTTGTAGTTTGACTTCTGCAGACCAGTTACAGACAATAGCTGATCATTCTTATTA
  TGAAGGATTATGTGAAGTAGTTCATCTTGCAGGCNNGGGGNAGACTCTAAAGCGGTATGG
  AGAAATCT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003473
• Insert Size: 2800 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003473.2, NP_003464.1
• RefSeq Size: 2967 bp
• RefSeq ORF: 1623 bp
• Locus ID: 8027
• UniProt ID: Q92783
• Cytogenetics: 10p12.33
• Domains: SH3, VHS, UIM
• Protein Pathways: Endocytosis, Jak-STAT signaling pathway
• Gene Summary: This gene encodes a member of the signal-transducing adaptor molecule family. These proteins mediate downstream signaling of cytokine receptors and also play a role in ER to Golgi trafficking by interacting with the coat protein II complex. The encoded protein also associates with hepatocyte growth factor-regulated substrate to form the endosomal sorting complex required for transport-0 (ESCRT-0), which sorts ubiquitinated membrane proteins to the ESCRT-1 complex for lysosomal degradation. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, Feb 2011]
  Transcript Variant: This variant (1) encodes the longest isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.