CXCR4 (NM_003467) Human Untagged Clone

CAT#: SC117951

CXCR4 (untagged)-Human chemokine (C-X-C motif) receptor 4 (CXCR4), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CXCR4
• Synonyms: CD184; D2S201E; FB22; HM89; HSY3RR; LAP-3; LAP3; LCR1; LESTR; NPY3R; NPYR; NPYRL; NPYY3R; WHIM; WHIMS
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_003467 edited
  GAATTCGGCACGAGGGCCGAGGGCCTGAGTGCTCCAGTAGCCACCGCATCTGGAGAACCA
  GCGGTTACCATGGAGGGGATCAGTATATACACTTCAGATAACTACACCGAGGAAATGGGC
  TCAGGGGACTATGACTCCATGAAGGAACCCTGTTTCCGTGAAGAAAATGCTAATTTCAAT
  AAAATCTTCCTGCCCACCATCTACTCCATCATCTTCTTAACTGGCATTGTGGGCAATGGA
  TTGGTCATCCTGGTCATGGGTTACCAGAAGAAACTGAGAAGCATGACGGACAAGTACAGG
  CTGCACCTGTCAGTGGCCGACCTCCTCTTTGTCATCACGCTTCCCTTCTGGGCAGTTGAT
  GCCGTGGCAAACTGGTACTTTGGGAACTTCCTATGCAAGGCAGTCCATGTCATCTACACA
  GTCAACCTCTACAGCAGTGTCCTCATCCTGGCCTTCATCAGTCTGGACCGCTACCTGGCC
  ATCGTCCACGCCACCAACAGTCAGAGGCCAAGGAAGCTGTTGGCTGAAAAGGTGGTCTAT
  GTTGGCGTCTGGATCCCTGCCCTCCTGCTGACTATTCCCGACTTCATCTTTGCCAACGTC
  AGTGAGGCAGATGACAGATATATCTGTGACCGCTTCTACCCCAATGACTTGTGGGTGGTT
  GTGTTCCAGTTTCAGCACATCATGGTTGGCCTTATCCTGCCTGGTATTGTCATCCTGTCC
  TGCTATTGCATTATCATCTCCAAGCTGTCACACTCCAAGGGCCACCAGAAGCGCAAGGCC
  CTCAAGACCACAGTCATCCTCATCCTGGCTTTCTTCGCCTGTTGGCTGCCTTACTACATT
  GGGATCAGCATCGACTCCTTCATCCTCCTGGAAATCATCAAGCAAGGGTGTGAGTTTGAG
  AACACTGTGCACAAGTGGATTTCCATCACCGAGGCCCTAGCTTTCTTCCACTGTTGTCTG
  AACCCCATCCTCTATGCTTTCCTTGGAGCCAAATTTAAAACCTCTGCCCAGCACGCACTC
  ACCTCTGTGAGCAGAGGGTCCAGCCTCAAGATCCTCTCCAAAGGAAAGCGAGGTGGACAT
  TCATCTGTTTCCACTGAGTCTGAGTCTTCAAGTTTTCACTCCAGCTAACACAGATGTAAA
  AGACTTTTTTTTATACGATAAATAACTTTTTTTTAAGTTACACATTTTTCAGATATAAAA
  GACTGACCAATATTGTACAGTTTTTATTGCTTGTTGGATTTTTGTCTTGTGTTTCTTTAG
  TTTTTGTGAAGTTTAATTGACTTATTTATATAAATTTTTTTTTGTTTCATATTGATGTGT
  GTCTAGGCAGGACCTGTGGCCAAGTTCTTAGTTGCTGTATGTCTCGTGGTAGGACTGTAG
  AAAAGGGAACTGAACATTCCAGAGCGTGTAGTGAATCACGTAAAGCTAGAAATGATCCCC
  AGCTGTTTATGCATAGATAATCTCTCCATTCCCGTGGAACGTTTTTCCTGTTCTTAAGAC
  GTGATTTTGCTGTAGAAGATGGCACTTATAACCAAAGCCCAAAGTGGTATAGAAATGCTG
  GTTTTTCAGTTTTCAGGAGTGGGTTGATTTCAGCACCTACAGTGTACAGTCTTGTATTAA
  GTTGTTAATAAAAGTACATGTTAAACTTAAAAAAAAAAAAAAAAAACTCGAC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003467 unedited
  TTACTATTTGTATACGACTCACTATAGGGNNCGGCCGCGCAATTCGGCACGAGGGCCGAG
  GGCCTGAGTGCTCCAGTAGCCACCGCATCTGGAGAACCAGCGGTTACCATGGAGGGGATC
  AGTATATACACTTCAGATAACTACACCGAGGAAATGGGCTCAGGGGACTATGACTCCATG
  AAGGAACCCTGTTTCCGTGAAGAAAATGCTAATTTCAATAAAATCTTCCTGCCCACCATC
  TACTCCATCATCTTCTTAACTGGCATTGTGGGCAATGGATTGGTCATCCTGGTCATGGGT
  TACCAGAAGAAACTGAGAAGCATGACGGACAAGTACAGGCTGCACCTGTCAGTGGCCGAC
  CTCCTCTTTGTCATCACGCTTCCCTTCTGGGCAGTTGATGCCGTGGCAAACTGGTACTTT
  GGGAACTTCCTATGCAAGGCAGTCCATGTCATCTACACAGTCAACCTCTACAGCAGTGTC
  CTCATCCTGGCCTTCATCAGTCTGGACCGCTACCTGGCCATCGTCCACGCCACCAACAGT
  CAGAGGCCAAGGAAGCTGTTGGCTGAAAAGGTGGTCTATGTTGGCATCTGGATCCCTGCC
  CTCCTGCTGACTATTCCCGACTTCATCTTTGCCAACGTCAGTGAGGCAGATGACAGATAT
  ATCTGTGACCGCTCCTACCCCATGACTTGTGGGTGTTTGTGTTCCAGTTTCAGCACATAA
  TGGTTTGGCCTTATCCTGCCTTGTTATGGCCATCCTGTCCTGCTATTGCATTATCATCTC
  CAAGCTGTTACATTCCAGGGGCCACAGAAAGCGCAAGCCCCTCAAGACCACAGGCATCCT
  CATCCTTGCTTTTTTCGCCTGTGGCTGGCTTACTACATTGGGTACAGGATTGACTCCTTA
  ATCCTCTGGAAAAATCAACCAAGGGGTGAG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003467 unedited
  TGGACCGCGGGCCGCAATCTANAGTCGAGTTTTTTTTTTTTTTTTTTAAGTTTAACATGT
  ACTTTTATTAACAACTTAATACAAGACTGTACACTGTAGGTGCTGAAATCAACCCACTCC
  TGAAAACTGAAAAACCAGCATTTCTATACCACTTTGGGCTTTGGTTATAAGTGCCATCTT
  CTACAGCAAAATCACGTCTTAAGAACAGGAAAAACGTTCCACGGGAATGGAGAGATTATC
  TATGCATAAACAGCTGGGGATCATTTCTAGCTTTACGTGATTCACTACACGCTCTGGAAT
  GTTCAGTTCCCTTTTCTACAGTCCTACCACGAGACATACAGCAACTAAGAACTTGGCCAC
  AGGTCCTGCCTAGACACACATCAATATGAAACAAAAAAAAATTTATATAAATAAGTCAAT
  TAAACTTCACAAAAACTAAAGAAACACAAGACAAAAATCCAACAAGCAATAAAAACTGTA
  CAATATTGGTCAGTCTTTTATATCTGAAAAATGTGTAACTTAAAAAAAAGTTATTTATCG
  TATAAAAAAAAGTCTTTTACATCTGTGTTAGCTGGAGTGAAAACTTGAAGACTCAGACTC
  AGTGGAAACAGATGAATGTCCACCTCGCTTTCCTTTGGAGAGGATCTTGAGGCTGGACCC
  TCTGCTCACAGAGGTGAGTGCGTGCTGGGCAAAGGTTTTAAATTTGGCTCCAAGGAAAGC
  ATAGAGGATGGGGTTCAGACAACAGTGGAAGAAAGCTAAGCCCCTCGTGATGGAAATCCA
  CTTGTGCACAGGGTTCTCAAACTCACACCCTTGCTTGATGATTCCCAGNAGGATGAAGGA
  GTCAATGCTGATCCCAATGTANTAAGGCAGCCAACCAGCGAAAAAGCCAGGATGAAGATG
  ACTGTGGTCTTGAG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003467
• Insert Size: 1720 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003467.2, NP_003458.1
• RefSeq Size: 1691 bp
• RefSeq ORF: 1059 bp
• Locus ID: 7852
• UniProt ID: P61073
• Cytogenetics: 2q22.1
• Domains: 7tm_1
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, GPCR, Transmembrane
• Protein Pathways: Axon guidance, Chemokine signaling pathway, Cytokine-cytokine receptor interaction, Endocytosis, Leukocyte transendothelial migration
• Gene Summary: This gene encodes a CXC chemokine receptor specific for stromal cell-derived factor-1. The protein has 7 transmembrane regions and is located on the cell surface. It acts with the CD4 protein to support HIV entry into cells and is also highly expressed in breast cancer cells. Mutations in this gene have been associated with WHIM (warts, hypogammaglobulinemia, infections, and myelokathexis) syndrome. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (2) lacks an alternate in-frame exon compared to variant 3. The resulting isoform (b) has the same N- and C-termini but is shorter compared to isoform c.