TIP49A (RUVBL1) (NM_003707) Human Untagged Clone

SKU SC117818

RUVBL1 (untagged)-Human RuvB-like 1 (E. coli) (RUVBL1)

Product Data

• Type: Human Untagged Clone
• Target Symbol: TIP49A
• Synonyms: ECP-54; ECP54; INO80H; NMP 238; NMP238; PONTIN; Pontin52; RVB1; TIH1; TIP49; TIP49A
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_003707, the custom clone sequence may differ by one or more nucleotides; ; ; ATGAAGATTGAGGAGGTGAAGAGCACTACGAAGACGCAGCGCATCGCCTCCCACAGCCACGTGAAAGGGC; TGGGGCTGGACGAGAGCGGCTTGGCCAAGCAGGCGGCCTCAGGGCTTGTGGGCCAGGAGAACGCGCGAGA; GGCATGTGGCGTCATAGTAGAATTAATCAAAAGCAAGAAAATGGCTGGAAGAGCTGTCTTGTTGGCAGGA; CCTCCTGGAACTGGCAAGACAGCTCTGGCTCTGGCTATTGCTCAGGAGCTGGGTAGTAAGGTCCCCTTCT; GCCCAATGGTGGGGAGTGAAGTTTACTCAACTGAGATCAAGAAGACAGAGGTGCTGATGGAGAACTTCCG; CAGGGCCATTGGGCTGCGAATAAAGGAGACCAAGGAAGTTTATGAAGGTGAAGTCACAGAGCTAACTCCG; TGTGAGACAGAGAATCCCATGGGAGGATATGGCAAAACCATTAGCCATGTGATCATAGGACTCAAAACAG; CCAAAGGAACCAAACAGTTGAAACTGGACCCCAGCATTTTTGAAAGTTTGCAGAAAGAGCGAGTAGAAGC; TGGAGATGTGATTTACATTGAAGCCAACAGTGGGGCCGTGAAGAGGCAGGGCAGGTGTGATACCTATGCC; ACAGAATTCGACCTTGAAGCTGAAGAGTATGTCCCCTTGCCAAAAGGGGATGTGCACAAAAAGAAAGAAA; TCATCCAAGATGTGACCTTGCATGACTTGGATGTGGCTAATGCGCGGCCCCAGGGGGGACAAGATATCCT; GTCCATGATGGGCCAGCTAATGAAGCCAAAGAAGACAGAAATCACAGACAAACTTCGAGGGGAGATTAAT; AAGGTGGTGAACAAGTACATCGACCAGGGCATTGCTGAGCTGGTCCCGGGTGTGCTGTTTGTTGATGAGG; TCCACATGCTGGACATTGAGTGCTTCACCTACCTGCACCGCGCCCTGGAGTCTTCTATCGCTCCCATCGT; CATCTTTGCATCCAACCGAGGCAACTGTGTCATCAGAGGCACTGAGGACATCACATCCCCTCACGGCATC; CCTCTTGACCTTCTGGACCGAGTGATGATAATCCGGACCATGCTGTATACTCCACAGGAAATGAAACAGA; TCATTAAAATCCGTGCCCAGACGGAAGGAATCAACATCAGTGAGGAGGCACTGAACCACCTGGGGGAGAT; TGGCACCAAGACCACACTGAGGTACTCAGTGCAGCTGCTGACCCCGGCCAACTTGCTTGCTAAAATCAAC; GGGAAGGACAGCATTGAGAAAGAGCATGTCGAAGAGATCAGTGAACTTTTCTATGATGCCAAGTCCTCCG; CCAAAATCCTGGCTGACCAGCAGGATAAGTACATGAAGTGA; ;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003707 unedited; GTATTTTGTAATACGACTCACTTATAGGGCGGCCGCGAATTCGCACCAGCTCAGGCGCCG; GGCGCACTGTCCTAGCTGCTGGTTTTCCACGCTGGTTTTAGCTCCCGGCGTCTGCAAAAT; GAAGATTGAGGAGGTGAAGAGCACTACGAAGACGCAGCGCATCGCCTCCCACAGCCACGT; GAAAGGGCTGGGGCTGGACGAGAGCGGCTTGGCCAAGCAGGCGGCCTCAGGGCTTGTGGG; CCAGGAGAACGCGCGAGAGGCATGTGGCGTCATAGTAGAATTAATCAAAAGCAAGAAAAT; GGCTGGAAGAGCTGTCTTGTTGGCAGGACCTCCTGGAACTGGCAAGACAGCTCTGGCTCT; GGCTATTGCTCAGGAGCTGGGTAGTAAGGTCCCCTTCTGCCCAATGGTGGGGAGTGAAGT; TTACTCAACTGAGATCAAGAAGACAGAGGTGCTGATGGAGAACTTCCGCAGGGCCATTGG; GCTGCGAATAAAGGAGACCAAGGAAGTTTATGAAGGTGAAGTCACAGAGCTAACTCCGTG; TGAGACAGAGAATCCCATGGGAGGATATGGCAAAACCATTAGCCATGTGATCATAGGACT; CAAAACAGCCAAAGGAACCAAACAGTTGAAACTGGACCCCAGCATTTTTGAAAGTTTGCA; GAAAGAGCGAGTAGAAGCTGGAGATGTGATTTACATTGAAGCCAACAGTGGGGCCGTGAA; GAGGCAGGGCAGGTGTGATACCTATGCCACAGAATTCGACCTTTGAGCTNGAGAGTATGT; CCCCCTTGCCAAAAGGGGATGTGCACAAAAAGAAAGAAACATCCAAGATGTGACCCTGCA; TGACTTGGATTGTGCTAATGCCGCGGCCCCAGGGGGGACAAGATATCCTGTCCATGATGG; GCCCAGCTATGAAGCCANAGNAGACAGAAATCACAGACAACTTCGAGGGGAGATAATAAG; GGGTGCACCAGTCATCGC;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003707 unedited; TGTACGCGGCCGCAATCTATATCGAGTTTTTTTTTTTTTTTTTTCTGTTCTGAAAATTTA; TTTTTAAAAAACTTATAGATATATATAGAATCAAAATAACCTATGAAGATTTATAGAAAA; CACACCAGGTAAGGAAGGGTTCTTTCAAAGCAACCACAGGAACAGTTACGATAACTTAAA; AAGAAATGCTTTCCACACTGAACTGACAGCGCTGCAGCCCACGCCTGAGTGGGGACGGCA; GCCCCAAGCCCAGGGGCAAGCGCCCACAGGCTGGACCCAGGCCAGGCACACCTGGGGAGT; CTCTTGCTGCTGAAAACCTCAGCCATCTCACTTCATGTACTTATCCTGCTGGTCAGCCAG; GATTTTGGCGGAGGACTTGGCATCATAGAAAAGTTCACTGATCTCTTCGACATGCTCTTT; CTCAATGCTGTCCTTCCCGTTGATTTTAGCAAGCAAGTTGGCCGGGGTCAGCAGCTGCAC; TGAGTACCTCAGTGTGGTCTTGGTGCCAATCTCCCCCAGGTGGTTCAGTGCCTCCTCACT; GATGTTGATTCCTTCCGTCTGGGCACGGATTTTAATGATCTGTTTCATTTCCTGTGGAGT; ATACAGCATGGTCCGGATTATCATCACTCGGTCCAGATGGTCAAGATGGATGCCGTGAGG; GGATGTGATGTCCTCAGTGCCTCTGATGACACAGTTGCCTCGGTTGGATGCAAAGATGAC; GATGGGAGCGATAGAATACTNCAGGGGCGCGTGCAGGTAGGTGAAGCACTCATTGTCCAA; CATGTGGGCCTCATCAACAAACAGCACACCCGGGACCACCTCAGCAATGCCCTGGTCGAT; GTACTTGTTAACCACCTTATTAATCTCCCCTCCAAGTTGGCCTGGGATTTCGGGCCTCTC; TGGGCTTCATAACCGGGCCATTATGGACAGGATTTTTGCCCCCCTTGGGGCGCGCTTTAC; CACTTCCAGTCTGGCAGTCCATTTTGGGAGATTTTT;
• Restriction Sites: NotI-NotI
• ACCN: NM_003707
• Insert Size: 1750 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_003707.1, NP_003698.1
• RefSeq Size: 1750 bp
• RefSeq ORF: 1371 bp
• Locus ID: 8607
• UniProt ID: Q9Y265
• Cytogenetics: 3q21.3
• Domains: AAA
• Protein Families: Stem cell - Pluripotency, Transcription Factors
• Protein Pathways: Wnt signaling pathway
• Summary: This gene encodes a protein that has both DNA-dependent ATPase and DNA helicase activities and belongs to the ATPases associated with diverse cellular activities (AAA+) protein family. The encoded protein associates with several multisubunit transcriptional complexes and with protein complexes involved in both ATP-dependent remodeling and histone modification. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2016]; Transcript Variant: This variant (1) encodes the longer isoform 1.