ALAD (NM_000031) Human Untagged Clone

CAT#: SC117775

ALAD (untagged)-Human aminolevulinate dehydratase (ALAD)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ALAD
• Synonyms: ALADH; PBGS
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_000031, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGCAGCCCCAGTCCGTTCTGCACAGCGGCTACTTCCACCCACTACTTCGGGCCTGGCAGACAGCCACCA
  CCACCCTCAATGCCTCCAACCTCATCTACCCCATCTTTGTCACGGATGTTCCTGATGACATACAGCCTAT
  CACCAGCCTCCCAGGAGTGGCCAGGTATGGTGTGAAGCGGCTGGAAGAGATGCTGAGGCCCTTGGTGGAA
  GAGGGCCTACGCTGTGTCTTGATCTTTGGCGTCCCCAGCAGAGTTCCCAAGGACGAGCGGGGTTCCGCAG
  CTGACTCCGAGGAGTCCCCAGCTATTGAGGCAATCCATCTGTTGAGGAAGACCTTCCCCAACCTCCTGGT
  GGCCTGTGATGTCTGCCTGTGTCCCTACACCTCCCATGGTCACTGCGGGCTCCTGAGTGAAAACGGAGCA
  TTCCGGGCTGAGGAGAGCCGCCAGCGGCTGGCTGAGGTGGCATTGGCGTATGCCAAGGCAGGATGTCAGG
  TGGTAGCCCCGTCGGACATGATGGATGGACGCGTGGAAGCCATCAAAGAGGCCCTGATGGCACATGGACT
  TGGCAACAGGGTATCGGTGATGAGCTACAGTGCCAAATTTGCTTCCTGTTTCTATGGCCCTTTCCGGGAT
  GCAGCTAAGTCAAGCCCAGCTTTTGGGGACCGCCGCTGCTACCAGCTGCCCCCTGGAGCACGAGGCCTGG
  CTCTCCGAGCTGTGGACCGGGATGTACGGGAAGGAGCTGACATGCTCATGGTGAAGCCGGGAATGCCCTA
  CCTGGACATCGTGCGGGAGGTAAAGGACAAGCACCCTGACCTCCCTCTCGCCGTGTACCACGTCTCTGGA
  GAGTTTGCCATGCTGTGGCATGGAGCCCAGGCCGGGGCATTTGATCTCAAGGCTGCCGTACTGGAGGCCA
  TGACTGCCTTCCGCAGAGCAGGTGCTGACATCATCATCACCTACTACACACCGCAGCTGCTGCAGTGGCT
  GAAGGAGGAATGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000031 unedited
  GTCAAAATTGTAAACGACTCACTATAGGCGGCCGCGAATTCGCACGAGCCAATGCCCCAG
  GAGCCCTCGGTTCCAACCAACTGATGCCCCTGTGCCCACTGGCCCACGCCATGCAGCCCC
  AGTCCGTTCTGCACAGCGGCTACTTCCACCCACTACTTCGGGCCTGGCAGACAGCCACCA
  CCACCCTCAATGCCTCCAACCTCATCTACCCCATCTTTGTCACGGATGTTCCTGATGACA
  TACAGCCTATCACCAGCCTCCCAGGAGTGGCCAGGTACGGTGTGAAGCGGCTGGAAGAGA
  TGCTGAGGCCCTTGGTGGAAGAGGGCCTACGCTGTGTCTTGATCTTTGGCGTCCCCAGCA
  GAGTTCCCAAGGACGAGCGGGGTTCCGCAGCTGACTCCGAGGAGTCCCCAGCTATTGAGG
  CAATCCATCTGTTGAGGAAGACCTTCCCCAACCTCCTGGTGGCCTGTGATGTCTGCCTGT
  GTCCCTACACCTCCCATGGTCACTGCGGGCTCCTGAGTGAAAACGGAGCATTCCGGGCTG
  AGGAGAGCCGCCAGCGGCTGGCTGAGGTGGCATTGGCGTATGCCAAGGCAGGATGTCAGG
  TGGTAGCCCCGTCGGACATGATGGATGGACGCGTGGAAGCCATCANAGAGGCCCTGATGG
  CACATGGACTTGGCAACAGGGTATCGGTGATGAGCTACAGTGCCAAATTTTGCTTCTGTT
  TCTATGGCCCTTTCCGGGATGCAGCTAAGTCAAGCCCAGCTTTTGGNGACCGNCGCTGCT
  ACCAGCTNGCCCCTGGAGCACGAAGCCTGGCTCTCCGAGCTGTGGACGGGNATGTACGGG
  AAGGAGCTGAATGCTCATGGTGAACCNGGNATGCCCTACCTGGACTCGTGCGGNAGGTAA
  AGACAAGACCCGGACTCCTTTTCGCGGTACACGTCTTGGAAATTTGCCGA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000031 unedited
  NNNNNCCCCCCCTAGGAGATGGCACTTCCAGGCCCAGAAAAGCACTGGGGNAGGGTCACA
  GGNATGCCACCCGGGTTCTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCG
  AGTTTTTTCTTTTTTTTTTATTGTACTTCAAAAATTGAATTTTTAATCAAAATTCTTTAT
  AAATTTATTACAATATAGATTTTGTGATGCATACCTTTGCCCACTGCAGGCTAATGTGAG
  TGTTCTGAGCAAGTTTAAGGCAGGCTAGGCTAACCTATGATGTTTGGAAGGTTAGGTATA
  TTAAATACATTTTTGACTCACGATCATATTTTCAACTTATGATCACATTTTCAACTTAGG
  ACGGGTTTATCAGGATGCAGACCCATCACATAAATGGAGGGGTGTCCATAAGACATTGCT
  AGACCTATAAATTTGCTGTTGAATAATGGGAGGTGGTATGGCACAGTGGTTAGGGGCATA
  GGCCTTGGAGTTGGACTACATGGGTTCAAATCCCAGCTTGGCTGTTTTCTGTGCAGTTCT
  AATCCAGTTCTGCCACAAACTTGTTGAGTGGCCTTAAGTGACTCACATTAGCACTCTAGA
  TATAGCTTCCTTATGTGGAAAAGGCGCAAGCAAACTTTAGTATTCTCCCTCCACCCCGAC
  CATGCTTCTGTTCCTCATCCAGGCTGCCCCATCTTAGTAAATGGACCATCAACCACCGCG
  AAGTCAAGCCAAATTAATCCTTCACATCGGATCTACCTTCTAAACACATCCCCGGTCCCA
  TTTCCCTTTCTCCATCGCACTGTCCCCGGTCCGGTCCATGACAAGAAGAATTTTCAAT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000031
• Insert Size: 3700 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000031.4, NP_000022.2
• RefSeq Size: 3151 bp
• RefSeq ORF: 1020 bp
• Locus ID: 210
• UniProt ID: P13716
• Cytogenetics: 9q32
• Domains: ALAD
• Protein Families: Druggable Genome
• Protein Pathways: Metabolic pathways, Porphyrin and chlorophyll metabolism
• Gene Summary: The ALAD enzyme is composed of 8 identical subunits and catalyzes the condensation of 2 molecules of delta-aminolevulinate to form porphobilinogen (a precursor of heme, cytochromes and other hemoproteins). ALAD catalyzes the second step in the porphyrin and heme biosynthetic pathway; zinc is essential for enzymatic activity. ALAD enzymatic activity is inhibited by lead and a defect in the ALAD structural gene can cause increased sensitivity to lead poisoning and acute hepatic porphyria. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Dec 2015]
  Transcript Variant: This variant (2) lacks a segment of the coding region and initiates translation from an alternate start codon, compared to variant 1. The resulting isoform (b) has a distinct N-terminus, compared to isoform a.