UBE2M (NM_003969) Human Untagged Clone

CAT#: SC117663

UBE2M (untagged)-Human ubiquitin-conjugating enzyme E2M (UBE2M)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: UBE2M
• Synonyms: hUbc12; UBC-RS2; UBC12
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_003969 edited
  CACGAGGAGGAGGCCGCAGCTGCGGGTCCGAGGAGCGGAGGCGACGCGGGCGGCGGCGGG
  GGGCCGGGTGGCCGGGGTCCCGGGCCCCGCGGCGGCGGCAGCGGCGGCGGCGGCGGCAGG
  ATGATCAAGCTGTTCTCGCTGAAGCAGCAGAAGAAGGAGGAGGAGTCGGCGGGCGGCACC
  AAGGGCAGCAGCAAGAAGGCGTCGGCGGCGCAGCTGCGGATCCAGAAGGACATAAACGAG
  CTGAACCTGCCCAAGACGTGTGATATCAGCTTCTCAGATCCAGACGACCTCCTCAACTTC
  AAGCTGGTCATCTGTCCTGATGAGGGCTTCTACAAGAGTGGGAAGTTTGTGTTCAGTTTT
  AAGGTGGGCCAGGGTTACCCGCATGATCCCCCCAAGGTGAAGTGTGAGACAATGGTCTAT
  CACCCCAACATTGACCTCGAGGGCAACGTCTGCCTCAACATCCTCAGAGAGGACTGGAAG
  CCAGTCCTTACGATAAACTCCATAATTTATGGCCTGCAGTATCTCTTCTTGGAGCCCAAC
  CCCGAGGACCCACTGAACAAGGAGGCCGCAGAGGTCCTGCAGAACAACCGGCGGCTGTTT
  GAGCAGAACGTGCAGCGCTCCATGCGGGGTGGCTACATCGGCTCCACCTACTTTGAGCGC
  TGCCTGAAATAGGGTTGGCGCATACCCACCCCCGCCACGGCCACAAGCCCTGGCATCCCC
  TGCAAATATTTATTGGGGGCCATGGGTAGGGGTTTGGGGGGGGCGGCCGGTGGGGGGGAT
  CCCCCTGCCTTGGCCTTGCCTCCCCTTCCTGCCACGTGCCCCTAGTTATTTTTTTTTTTT
  TAACACCATGTGATTAAAGGTCGGCGCTGCCTCCCCCGACCCACTCAGCGATGGGAAATG
  AATTGGCTTGTCTAGC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003969 unedited
  TTGTACTACGATCTCACTATAGGGCGGCCGCGAATTCGCACGAGGAGGAGGCCGCAGCTG
  CGGGTCCGAGGAGCGGAGGCGACGCGGGCGGCGGCGGGGGGCCGGGTGGCCGGGGTCCCG
  GGCCCCGCGGCGGCGGCAGCGGCGGCGGCGGCGGCAGGATGATCAAGCTGTTCTCGCTGA
  AGCAGCAGAAGAAGGAGGAGGAGTCGGCGGGCGGCACCAAGGGCAGCAGCAAGAAGGCGT
  CGGCGGCGCAGCTGCGGATCCAGAAGGACATAAACGAGCTGAACCTGCCCAAGACGTGTG
  ATATCAGCTTCTCAGATCCAGACGACCTCCTCAACTTCAAGCTGGTCATCTGTCCTGATG
  AGGGCTTCTACAAGAGTGGGAAGTTTGTGTTCAGTTTTAAGGTGGGCCAGGGTTACCCGC
  ATGATCCCCCCAAGGTGAAGTGTGAGACAATGGTCTATCACCCCAACATTGACCTCGAGG
  GCAACGTCTGCCTCAACATCCTCAGAGAGGACTGGAAGCCAGTCCTTACGATAAACTCCA
  TAATTTATGGCCTGCAGTATCTCTTCTTGGAGCCCAACCCCGAGGACCCACTGAACAAGG
  AGGCCGCAGAGGTCCTGCAGAACAACCGGCGGCTGTTTGAGCAGAACGTGCAGCGCTCCA
  TGCGGNGTGGCTACATCGGCTCCACCTACTTTGAGCGCTGCCTGAAATAGGGTTGGCGCA
  TACCCACCCCCGCCACGGCCACAAGCCCTGGCATCCCCTGCAATATTATTGGNGGCCATG
  GTAGGGTNTTGGGGGGGGCGGCCGGTGGGGGAATCCCCTGCCTGGNCTTGCCTCCCCTTC
  TGCACGTGCCCTAGTATTTTTTTTTTTTACACATGGATAAGGTCGCGCTGCTCCCCGACC
  ATCGGATGGAATGGATTGGCTGCTACCCCCTGCTGGG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003969 unedited
  TGAACCGCGGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGCAGTAACATTCC
  CCTTTAATAGCCTGGTGGGACCTCCAGAGGTGGAGGGGTGGGTTGCCTGGGCCCAGCTAC
  CCATGCCCGTTGCACACTTTTCTTTNTTGTTAAAAAGTAGTAAAAAAAAAAAATCAAGGA
  AGATTGTATAATATTAAACATTAACGTCCGGTATATATATAATATTATTTTTTTATAATA
  CTTAACGTCGATGCGCCTCACTACATTTCAATATTCAAAAGGTAGAACAAACAAGAACGG
  AAGCGCGCCCAACATGGACCGAGCGAGTAGAAAATATAAAAACTATCTTATGCATGTTTA
  AACAATTACTATTTTTTCTCTCTTTCCTCCTCCCCTCCTCCACCCCACGCCACCGCTACC
  CAACGAACCCAAACTCCCCCTATATACCAAAGCCAACTCCCTCCCCACCCTTTTCTTCTT
  GCACATCCCAAGAGTCCGAAATTCATACACCCACCTCACTTGGCACCCCAACACCTACCC
  ACTACCCAACGAAGTCTTACATAATATTGACACGGTCCTATGACCTATACGACAAGCAAT
  AGTCTACTATCATTTTTATATAACAACAAACCTAACCCACAAGTATCTCACACGCCCATA
  ACCCCTATGTCCCAACACACGCCACATCACACCTCACCCTCACTTTATATTCAAATTCAA
  ACCTCAACATACAATGATAACATCTTATACAAATTCTATAGTAATTTTAATTTCATATAG
  CACTAAATACTTTCACGCGACAGCTCGACGGTTGTCATCTCTTCATGTTTGCGACCTGTT
  CTATACTCTCTTCTATATCTTATTATTACTTCCCTCTTCAGCTCTGCAGCTCGAGTGTCA
  CACCCCTTGACTGCGCACTCGATTACATAATATATAGTGACGCGCGAAAGACGCACGGCA
  GCGACTAAGACGACCAACCAGCACTACAAGCACACTCCAACGACTCTATTTCATTTAACA
  CCTATACTCGGT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003969
• Insert Size: 980 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003969.2, NP_003960.1
• RefSeq Size: 1536 bp
• RefSeq ORF: 552 bp
• Locus ID: 9040
• UniProt ID: P61081
• Cytogenetics: 19q13.43
• Domains: UBCc
• Protein Pathways: Ubiquitin mediated proteolysis
• Gene Summary: The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein is linked with a ubiquitin-like protein, NEDD8, which can be conjugated to cellular proteins, such as Cdc53/culin. [provided by RefSeq, Jul 2008]