CCBL1 (KYAT1) (NM_004059) Human Untagged Clone

SKU SC117604

CCBL1 (untagged)-Human cysteine conjugate-beta lyase, cytoplasmic (CCBL1), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: CCBL1
• Synonyms: CCBL1; GTK; KAT1; KATI
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC117604 sequence for NM_004059 edited (data generated by NextGen Sequencing); ATGGCCAAACAGCTGCAGGCCCGAAGGCTAGACGGGATCGACTACAACCCCTGGGTGGAG; TTTGTGAAACTGGCCAGTGAGCATGACGTCGTGAACTTGGGCCAGGGCTTCCCGGATTTC; CCACCACCAGACTTTGCCGTGGAAGCCTTTCAGCACGCTGTCAGTGGAGACTTCATGCTT; AACCAGTACACCAAGACATTTGGTTACCCACCACTGACGAAGATCCTGGCAAGTTTCTTT; GGGGAGCTGCTGGGTCAGGAGATAGACCCGCTCAGGAATGTGCTGGTGACTGTTGGTGGC; TATGGGGCCCTGTTCACAGCCTTCCAGGCCCTGGTGGACGAAGGAGACGAGGTCATCATC; ATCGAACCCTTTTTTGACTGCTACGAGCCCATGACAATGATGGCAGGGGGTCGTCCTGTG; TTTGTGTCCCTGAAGCCGGGTCCCATCCAGAATGGAGAACTGGGTTCCAGCAGCAACTGG; CAGCTGGACCCCATGGAGCTGGCCGGCAAATTCACATCACGCACCAAAGCCCTGGTCCTC; AACACCCCCAACAACCCCCTGGGCAAGGTGTTCTCCAGGGAAGAGCTGGAGCTGGTGGCC; AGCCTTTGCCAGCAGCATGACGTGGTGTGTATCACTGATGAAGTCTACCAGTGGATGGTC; TACGACGGGCACCAGCACATCAGCATTGCCAGCCTCCCTGGCATGTGGGAACGGACCCTG; ACCATCGGCAGCGCCGGCAAGACCTTCAGCGCCACTGGCTGGAAGGTGGGCTGGGTCCTG; GGTCCAGATCACATCATGAAGCACCTGCGGACCGTGCACCAGAACTCCGTCTTCCACTGC; CCCACGCAGAGCCAGGCTGCAGTAGCCGAGAGCTTTGAACGGGAGCAGCTGCTCTTCCGC; CAACCCAGCAGCTACTTTGTGCAGTTCCCGCAGGCCATGCAGCGCTGCCGTGACCACATG; ATACGTAGCCTACAGTCAGTGGGCCTGAAGCCCATCATCCCTCAGGGCAGCTACTTCCTC; ATCACAGACATCTCAGACTTCAAGAGGAAGATGCCTGACTTGCCTGGAGCTGTGGATGAG; CCCTATGACAGACGCTTCGTCAAGTGGATGATCAAGAACAAGGGCTTGGTGGCCATCCCT; GTCTCCATCTTCTATAGTGTGCCACATCAGAAGCACTTTGACCACTATATCCGCTTCTGT; TTTGTGAAGGATGAAGCCACGCTCCAGGCCATGGACGAGAAGCTGCGGAAGTGGAAGGTG; GAACTCTAG; ; Clone variation with respect to NM_004059.4;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004059 unedited; TTACCCCCGCCCCGTTGNCGCAAAGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAG; CAGAGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCGC; GAATTCGGCACGAGGTGGGAACGAGGGGTGAAGCGGCCCAGGTGAGGCTCGCCATGGCCA; AACAGCTGCAGGCCCGAAGGCTAGACGGGATCGACTACAACCCCTGGGTGGAGTTTGTGA; AACTGGCCAGTGAGCATGACGTCGTGAACTTGGGCCAGGGCTTCCCGGATTTCCCACCAC; CAGACTTTGCCGTGGAAGCCTTTCAGCACGCTGTCAGTGGAGACTTCATGCTTAACCAGT; ACACCAAGACATTTGGTTACCCACCACTGACGAAGATCCTGGCAAGTTTCTTTGGGGAGC; TGCTGGGTCAGGAGATAGACCCGCTCAGGAATGTGCTGGTGACTGTTGGTGGCTATGGGG; CCCTGTTCACAGCCTTCCAGGCCCTGGTGGACGAAGGAGACGAGGTCATCATCATCGAAC; CCTTTTTTGACTGCTACGAGCCCATGACAATGATGGCAGGGGGTCGTCCTGTGTTTGTGT; CCCTGAAGCCGGGTCCCATCCAGAATGGAGAACTGGGTTCCAGCAGCAACTGGCAGCTGG; ACCCCATGGNAGCTGCCGGCANATTCACATCACGCACCANAGCCCTGGTCCTCAACACCC; CCAACAACCCCCTGGGCAAGGGTGNTCTCCAGGNAAAGAGCTGAGCTGGTGGCCAGCCTT; TGCCAGCAGCATGACGTGGTGTGTATCACTGATGAAGTCTACCAGTGGATGGTCTACGAC; GGGCACAGCACATCAGCATTGCCAGCCTNCCTGGCATGTTGGAAACGGACCCTGACCCAT; CGNAGNNGCNGGCAGAACTTCAGCGCCACTGGGCTGAAAGTGGGGCTGGNTCCCTGGGTC; CAGAAACATNATGAAGCACA;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004059 unedited; NCGGAAATACTATGNTACCGCGGCCGCAATTCTANATCGAGTTTTTTTTTTTTTTTTTTT; TTTTTTTTTTTTTTTTTTTTTTTTGGTAAGGATCTTAATAACTTAAACATTTTATTATGG; ACCAAAAGGAAAGGCTTGAGGACTCAAGCCTAAAGGCAACTCCCCAAGGCCAAAATGAAC; CAGGGAAGGGGAGGTTATACCTGAACCTTAACAGGGGCCATGCATAACTGGGATGTGATC; GGTACATGGGGGAAGTCTACCGTCCGTCTTAACCAAACCTGGAGAGACCAGAAGCAACAC; AAGACCCTTCAAACCCACCTATGGAGGGGCAAGGAGAGGCCCAAGTCAAGCCACCCTCAC; CTTTTAAACAGGAGGCACTTGGTTCTCTAAAAAGAAAAAAGGGGGGTCCCACCCAAAACA; TTTTGTGTCACGGAAAAAAAGGTTCCCAATAACATCTTCCCCAACCTAAAAATGTCTGAA; ACCTGGACAAAAACAGACACTCCAAGAGGATCTCTGCGGGCATGTGGGGATGTCAAGGGC; CAAGCGTGACTTTAGGGGTAGAGTTTCACCTTCCACTTTCGCAACTTTTTGTCCATGGGC; CGGAACGTGGCTTTATTCTTTACAAAACAAAAACCGATATAGTGGGCAAAATGCTTCTGA; TGTGGCGCACTATATAAAAAGGGGACACGGGTGGGCACCAAACGCCTGGTTCTTGATCAT; CCCCTTTACCAAACCTCTGTCATAAGGGTTATTCACAGGTTCCGGGAAGTTAGGGAATTT; TCTCTTTAAAACTTAAAAGTCTGTGGAGAAGAAAAAACTGCCCCCGAGGAAGAATGGGCT; TTCAAGCCCACTGCCTGGGGGCTACCTTTCATATTGTCCCCGGAACCCTTCTTGGCTT;
• Restriction Sites: NotI-NotI
• ACCN: NM_004059
• Insert Size: 2000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_004059.3, NP_004050.2
• RefSeq Size: 2152 bp
• RefSeq ORF: 753 bp
• Locus ID: 883
• UniProt ID: Q16773
• Cytogenetics: 9q34.11
• Domains: aminotran_1_2
• Summary: This gene encodes a cytosolic enzyme that is responsible for the metabolism of cysteine conjugates of certain halogenated alkenes and alkanes. This metabolism can form reactive metabolites leading to nephrotoxicity and neurotoxicity. Increased levels of this enzyme have been linked to schizophrenia. Multiple transcript variants that encode different isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]; Transcript Variant: This variant (1) encodes the isoform a. Variants 1, 2, and 6-11 all encode the same isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The extent of this transcript is supported by transcript alignments.