Calpain 5 (CAPN5) (NM_004055) Human Untagged Clone

SKU SC117602

CAPN5 (untagged)-Human calpain 5 (CAPN5)

Product Data

• Type: Human Untagged Clone
• Target Symbol: Calpain 5
• Synonyms: ADNIV; HTRA3; nCL-3; VRNI
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_004055 edited; TCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCG; GCACGAGGGCTGCTGCGCCGGGCGGCTGCAGCCTCCGCTCCAGCGCCCGCGCCGTGCCCT; GCATCCCGGGAGCCCGGCTGGACCCGCGGCGGCTCGGCCGGTGTTCCCCCTCCCCTCCCT; GGGGCAGCAGCCACCATGTTCTCGTGTGTGAAGCCCTATGAGGACCAGAACTACTCAGCC; CTGAGGCGGGACTGCCGGCGCAGGAAGGTGCTCTTCGAGGACCCCCTCTTCCCCGCCACT; GACGACTCACTCTACTATAAGGGCACGCCGGGGCCCGCCGTCAGGTGGAAGCGACCCAAG; GGCATCTGCGAGGACCCCCGCCTCTTTGTGGATGGCATCAGCTCCCACGACCTGCACCAG; GGCCAGGTGGGCAACTGCTGGTTTGTGGCAGCCTGCTCGTCACTTGCCTCCCGGGAGTCG; CTGTGGCAAAAGGTCATCCCAGACTGGAAGGAGCAGGAATGGGACCCCGAAAAGCCCAAC; GCCTACGCGGGCATCTTCCACTTCCACTTCTGGCGCTTCGGGGAATGGGTGGACGTGGTC; ATCGATGACCGGCTGCCCACAGTCAACAACCAGCTCATCTACTGCCACTCCAACTCCCGC; AATGAGTTTTGGTGCGCCCTAGTGGAGAAGGCCTATGCCAAACTGGCAGGCTGTTACCAG; GCCCTGGATGGAGGCAACACAGCAGACGCACTGGTGGACTTCACGGGTGGTGTTTCTGAG; CCCATCGACCTGACCGAGGGTGACTTTGCCAACGATGAGACTAAGAGGAACCAGCTCTTT; GAGCGCATGTTAAAGGTGCACAGCCGGGGCGGCCTCATCAGTGCCTCCATCAAGGCAGTG; ACAGCAGCTGACATGGAGGCCCGCCTGGCGTGCGGCCTGGTAAAGGGCCACGCATACGCC; GTCACTGATGTGCGCAAGGTGCGCCTGGGCCACGGCCTACTGGCCTTCTTCAAGTCAGAG; AAGTTGGACATGATCCGCCTGCGCAACCCCTGGGGCGAGCGGGAGTGGAACGGGCCCTGG; AGTGACACCTCGGAGGAGTGGCAGAAAGTGAGCAAGAGTGAGCGGGAGAAGATGGGTGTG; ACCGTGCAGGACGACGGTGAGTTCTGGATGACCTTCGAGGACGTGTGCCGGTACTTCACG; GACATCATCAAGTGCCGCGTGATCAACACATCCCACCTGAGCATCCACAAGACGTGGGAG; GAGGCCCGGCTGCATGGCGCCTGGACGCTGCATGAGGACCCGCGACAGAACCGCGGTGGC; GGCTGCATCAACCACAAGGACACCTTCTTCCAGAACCCACAGTACATCTTCGAAGTCAAG; AAGCCAGAAGATGAAGTCCTGATCTGCATCCAGCAGCGGCCAAAGCGGTCTACGCGCCGG; GAGGGCAAGGGTGAGAACCTGGCCATTGGCTTTGACATCTACAAGGTGGAGGAGAACCGC; CAGTACCGCATGCACAGCCTGCAGCACAAGGCCGCCAGCTCCATCTACATCAACTCACGC; AGCGTCTTCCTGCGCACCGACCAGCCCGAGGGCCGCTATGTCATCATCCCCACAACCTTC; GAGCCAGGCCACACTGGCGAGTTCCTGCTCCGAGTCTTCACTGATGTGCCCTCCAACTGC; CGGGAGCTGCGCCTGGATGAGCCCCCACACACCTGCTGGAGCTCCCTCTGTGGCTACCCC; CAGCTGGTGACCCAGGTACATGTCCTGGGAGCTGCTGGCCTCAAGGACTCCCCAACAGGG; GCTAACTCTTATGTGATCATCAAGTGTGAGGGAGACAAAGTCCGCTCGGCTGTGCAGAAG; GGCACCTCCACACCAGAGTACAATGTGAAAGGCATCTTCTACCGCAAGAAGCTGAGCCAG; CCCATCACTGTACAGGTCTGGAACCACCGAGTGCTGAAGGATGAATTTCTGGGCCAGGTG; CACCTAAAGGCTGACCCGGACAACCTCCAGGCCCTGCATACCCTCCACCTCCGGGACCGA; AATAGCCGGCAGCCCAGCAACCTGCCAGGCACTGTGGCCGTGCACATTCTCAGCAGCACC; TCCCTCATGGCTGTCTGACACCTGCCCACCTACCTGGCTCTGACCGTTCCCACCACCATC; TGCATGTCCCCACTGGGCCTGAGTCTAGCCTGGGAGCCAGGATACTGGGGTCCTTTTCCC; ACTCTTCCACTGACTTGCTGTGTGACCTTAGGAAGTCTCTGCCCCTCTCTCAGCCTCAGT; GTCCCGAGGGCCCCGAAGCATTCCATTCTCGTGGAGGAGTTTCCTTGCTGAGATTTCAAA; TAGTCCTCCCCACCTCAACTGTCACCACTGCTA;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004055 unedited; CACGAGGCTGCTGCGCCGGGCGGCTGCAGCCTCCGCTCCAGCGCCCGCGCCGTGCCCTGC; ATCCCGGGAGCCCGGCTGGACCCGCGGCGGCTCGGCCGGTGTTCCCCCTCCCCTCCCTGG; GGCAGCAGCCACCATGTTCTCGTGTGTGAAGCCCTATGAGGACCAGAACTACTCAGCCCT; GAGGCGGGACTGCCGGCGCAGGAAGGTGCTCTTCGAGGACCCCCTCTTCCCCGCCACTGA; CGACTCACTCTACTATAAGGGCACGCCGGGGCCCGCCGTCAGGTGGAAGCGACCCAAGGG; CATCTGCGAGGACCCCCGCCTCTTTGTGGATGGCATCAGCTCCCACGACCTGCACCAGGG; CCAGGTGGGCAACTGCTGGTTTGTGGCAGCCTGCTCGTCACTTGCCTCCCGGGAGTCGCT; GTGGCAAAAGGTCATCCCAGACTGGAAGGAGCAGGAATGGGACCCCGAAAAGCCCAACGC; CTACGCGGGCATCTTCCACTTCCACTTCTGGCGCTTCGGGGAATGGGTGGACGTGGTCAT; CGATGACCGGCTGCCCACAGTCAACAACCAGCTCATCTACTGCCACTCCAACTCCCGCAA; TGAGTTTTGGTGCGCCCTAGTGGAGAAGGCCTATGCCAAACTGGCAGGCTGTTACCAGGC; CCTGGATGGAGGCAACACAGCAGACGCACTGGTGGACTTCACGG;
• Restriction Sites: NotI-NotI
• ACCN: NM_004055
• Insert Size: 2800 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_004055.3, NP_004046.2
• RefSeq Size: 3275 bp
• RefSeq ORF: 1923 bp
• Locus ID: 726
• UniProt ID: O15484
• Cytogenetics: 11q13.5
• Domains: C2, Calpain_III
• Protein Families: Druggable Genome, Protease
• Summary: Calpains are calcium-dependent cysteine proteases involved in signal transduction in a variety of cellular processes. A functional calpain protein consists of an invariant small subunit and 1 of a family of large subunits. CAPN5 is one of the large subunits. Unlike some of the calpains, CAPN5 and CAPN6 lack a calmodulin-like domain IV. Because of the significant similarity to Caenorhabditis elegans sex determination gene tra-3, CAPN5 is also called as HTRA3. [provided by RefSeq, Jul 2008]