EAAT3 (SLC1A1) (NM_004170) Human Untagged Clone

SKU SC117563

SLC1A1 (untagged)-Human solute carrier family 1 (neuronal/epithelial high affinity glutamate transporter, system Xag), member 1 (SLC1A1)

Product Data

• Type: Human Untagged Clone
• Target Symbol: EAAT3
• Synonyms: DCBXA; EAAC1; EAAT3; SCZD18
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC117563 sequence for NM_004170 edited (data generated by NextGen Sequencing); ATGGGGAAACCGGCGAGGAAAGGATGCGAGTGGAAGCGCTTCCTGAAGAATAACTGGGTG; TTGCTGTCCACCGTGGCCGCGGTGGTGCTAGGCATTACCACAGGAGTCTTGGTTCGAGAA; CACAGCAACCTCTCAACTCTAGAGAAATTCTACTTTGCTTTTCCTGGAGAAATTCTAATG; CGGATGCTGAAACTCATCATTTTGCCATTAATTATATCCAGCATGATTACAGGTGTTGCT; GCACTGGATTCCAACGTATCCGGAAAAATTGGTCTGCGCGCTGTCGTGTATTATTTCTGT; ACCACTCTCATTGCTGTTATTCTAGGTATTGTGCTGGTGGTGAGCATCAAGCCTGGTGTC; ACCCAGAAAGTGGGTGAAATTGCGAGGACAGGCAGCACCCCTGAAGTCAGTACGGTGGAT; GCCATGTTAGATCTCATCAGGAATATGTTCCCTGAGAATCTTGTCCAGGCCTGTTTTCAG; CAGTACAAAACTAAGCGTGAAGAAGTGAAGCCTCCCAGCGATCCAGAGATGAACATGACA; GAAGAGTCCTTCACAGCTGTCATGACAACTGCAATTTCCAAGAACAAAACAAAGGAATAC; AAAATTGTTGGCATGTATTCAGATGGCATAAACGTCCTGGGCTTGATTGTCTTTTGCCTT; GTCTTTGGACTTGTCATTGGAAAAATGGGAGAAAAGGGACAAATTCTGGTGGATTTCTTC; AATGCTTTGAGTGATGCAACCATGAAAATCGTTCAGATCATCATGTGTTATATGCCACTA; GGTATTTTGTTCCTGATTGCTGGGAAGATCATAGAAGTTGAAGACTGGGAAATATTCCGC; AAGCTGGGCCTTTACATGGCCACAGTCCTGACTGGGCTTGCAATCCACTCCATTGTAATT; CTCCCGCTGATATATTTCATAGTCGTACGAAAGAACCCTTTCCGATTTGCCATGGGAATG; GCCCAGGCTCTCCTGACAGCTCTCATGATCTCTTCCAGTTCAGCAACACTGCCTGTCACC; TTCCGCTGTGCTGAAGAAAATAACCAGGTGGACAAGAGGATCACTCGATTCGTGTTACCC; GTTGGTGCAACAATCAACATGGATGGGACCGCGCTCTATGAAGCAGTGGCAGCGGTGTTT; ATTGCACAGTTGAATGACCTGGACTTGGGCATTGGGCAGATCATCACCATCAGTATCACG; GCCACATCTGCCAGCATCGGAGCTGCTGGCGTGCCCCAGGCTGGCCTGGTGACCATGGTG; ATTGTGCTGAGTGCCGTGGGCCTGCCCGCCGAGGATGTCACCCTGATCATTGCTGTCGAC; TGGCTCCTGGACCGGTTCAGGACCATGGTCAACGTCCTTGGTGATGCTTTTGGGACGGGC; ATTGTGGAAAAGCTCTCCAAGAAGGAGCTGGAGCAGATGGATGTTTCATCTGAAGTCAAC; ATTGTGAATCCCTTTGCCTTGGAATCCACAATCCTTGACAACGAAGACTCAGACACCAAG; AAGTCTTATGTCAATGGAGGCTTTGCAGTAGACAAGTCTGACACCATCTCATTCACCCAG; ACCTCACAGTTCTAG; ; Clone variation with respect to NM_004170.5; 1110 t=>c
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004170 unedited; AGCTCTGGATTTTGTAATACGACTCACTATAGGGCGGCCGACGAATTCGCACGAGGCGAC; AGCCATGGNGGAAACCGGCGAGGAAAGGATGCGAGTGGAAGCGCTTCCTGAAGAATAACT; GGGTGTTGCTGTCCACCGTGGCCGCGGTGGTGCTAGGCATTACCACAGGAGTCTTGGTTC; GAGAACACAGCAACCTCTCAACTCTAGAGAAATTCTACTTTGCTTTTCCTGGAGAAATTC; TAATGCGGATGCTGAAACTCATCATTTTGCCATTAATTATATCCAGCATGATTACAGGTG; TTGCTGCACTGGATTCCAACGTATCCGGAAAAATTGGTCTGCGCGCTGTCGTGTATTATT; TCTGTACCACTCTCATTGCTGTTATTCTAGGTATTGTGCTGGTGGTGAGCATCAAGCCTG; GTGTCACCCAGAAAGTGGGTGAAATTGCGAGGACAGGCAGCACCCCTGAAGTCAGTACGG; TGGATGCCATGTTAGATCTCATCAGGAATATGTTCCCTGAGAATCTTGTCCAGGCCTGTT; TTCAGCAGTACAAAACTAAGCGTGAAGAAGTGAAGCCTCCCAGCGATCCAGAGATGAACA; TGACAGAAGAGTCCTTCACAGCTGTCATGACAACTGCAATTTCCAAGAACAAAACAAAGG; AATACAAAATTGTTGGCATGTATTCAGATGGCATAAACGTCCTGNGCTTGATTGTCTTTT; GCCTTGTCTTTGGACTTGTCATTGGAAAAATGGGAGAAAAGGGACAAATTCTGGTGGATT; TCTTCATGCTTTGAGTGATGCAACCATGAAAATCGTTCAGATCATCATGTGTTATATGCC; ACTANGTATTTTGTTCCTGATTGCTGGGAAGATCATAGAAGTTNGAGACTGGGGAATATT; NCGCAAGCTGGGCCTTTACATGGCC;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004170 unedited; CGCGGGCCGCAATCTAGNATCGAGTTTTTTTTTTTTTTTTTTAATTTATATTTAGATTTA; TTTCCTATAAACAATAGCAATATTTGCATTTTAAAATTCACATTGAATCATTATTTACTA; TTTATGATGTTTACATAACAATTCAGTATCATTATGTTGTAGATTTCAGATGTAGGTCGT; CAATACTGAGCACTTATCTACTCTTCTGCTATCTTTGGCACAATTTCTCATTTCTTAAAC; ATGAAAGCACAGGTTTAAGTTGGCTACAGAATACTGCAGAAACATGACTATTTTGGATTT; CAGTGTAAGTACAAGTTTATGTTAAGCATTATTTACACGATGAGAACTGAAGTACAGACT; ATAATAAACTATCTTCATAAATTACCAGAATGACAAGCAGAGGATGTAATGAATAGGGCC; AAGATTACTAGTTAGAACAGTCCTCCTGGAGGAGGAGACAAGAGTCTTAGAATGGAACTA; TTTCCCCCGGACCACTCATACCAGCTTATGTCGTGGGATTTACTCTGCAACAACCCAGGT; TATGGGGAAGCTCTTTTTNTTGCAACGCGGCTTGTTCCACCCATGTGATCTTTATTAAAA; CACATTTTACTTGTTACCTTGCCACTGCTATTCCGCGCCTGATAACACATCTGAAGGCAG; GCAAAGAACTCGCCTCTTTGAGTTGAATGAACTGGGAACATAAAGCCTTCATCTGGATGA; AGCCCCTCCGCCTTTCGTGGAGGCCAGCTTCTTATCCACCCTACGCCAACCGTATTGGGT; CCCCCCCAGCCCCCCAATTTTGAACCCTGTAAAGATGGCTAAGCCACCTCGGGTCACACC; CTATTACTCTTTGTCTTAAGTCCCTACCTCTGGAAACCTTTACCACTGATTCTTTTCTAG; TGGCTATAAAAGGCACCTCTTACATTTACCCACCTGGCTGGATACGGTCTTTATACATTC; GAGACCCCTCCTTTCCCCTCGGTTTTTTCCCTTCCTCGCACCC;
• Restriction Sites: NotI-NotI
• ACCN: NM_004170
• Insert Size: 3370 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_004170.4, NP_004161.4
• RefSeq Size: 3730 bp
• RefSeq ORF: 1575 bp
• Locus ID: 6505
• UniProt ID: P43005
• Cytogenetics: 9p24.2
• Domains: SDF
• Protein Families: Druggable Genome, Transmembrane
• Summary: This gene encodes a member of the high-affinity glutamate transporters that play an essential role in transporting glutamate across plasma membranes. In brain, these transporters are crucial in terminating the postsynaptic action of the neurotransmitter glutamate, and in maintaining extracellular glutamate concentrations below neurotoxic levels. This transporter also transports aspartate, and mutations in this gene are thought to cause dicarboxylicamino aciduria, also known as glutamate-aspartate transport defect. [provided by RefSeq, Mar 2010]