EAAT1 (SLC1A3) (NM_004172) Human Untagged Clone

SKU SC117514

SLC1A3 (untagged)-Human solute carrier family 1 (glial high affinity glutamate transporter), member 3 (SLC1A3), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: EAAT1
• Synonyms: EA6; EAAT1; GLAST; GLAST1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC117514 sequence for NM_004172 edited (data generated by NextGen Sequencing); ATGACTAAAAGCAATGGAGAAGAGCCCAAGATGGGGGGCAGGATGGAGAGATTCCAGCAG; GGAGTCCGTAAACGCACACTTTTGGCCAAGAAGAAAGTGCAGAACATTACAAAGGAGGAT; GTTAAAAGTTACCTGTTTCGGAATGCTTTTGTGCTGCTCACAGTCACCGCTGTCATTGTG; GGTACAATCCTTGGATTTACCCTCCGACCATACAGAATGAGCTACCGGGAAGTCAAGTAC; TTCTCCTTTCCTGGGGAACTTCTGATGAGGATGTTACAGATGCTGGTCTTACCACTTATC; ATCTCCAGTCTTGTCACAGGAATGGCGGCGCTAGATAGTAAGGCATCAGGGAAGATGGGA; ATGCGAGCTGTAGTCTATTATATGACTACCACCATCATTGCTGTGGTGATTGGCATAATC; ATTGTCATCATCATCCATCCTGGGAAGGGCACAAAGGAAAACATGCACAGAGAAGGCAAA; ATTGTACGAGTGACAGCTGCAGATGCCTTCCTGGACTTGATCAGGAACATGTTCCCTCCA; AATCTGGTAGAAGCCTGCTTTAAACAGTTTAAAACCAACTATGAGAAGAGAAGCTTTAAA; GTGCCCATCCAGGCCAACGAAACGCTTGTGGGTGCTGTGATAAACAATGTGTCTGAGGCC; ATGGAGACTCTTACCCGAATCACAGAGGAGCTGGTCCCAGTTCCAGGATCTGTGAATGGA; GTCAATGCCCTGGGTCTAGTTGTCTTCTCCATGTGCTTCGGTTTTGTGATTGGAAACATG; AAGGAACAGGGGCAGGCCCTGAGAGAGTTCTTTGATTCTCTTAACGAAGCCATCATGAGA; CTGGTAGCAGTAATAATGTGGTATGCCCCCGTGGGTATTCTCTTCCTGATTGCTGGGAAG; ATTGTGGAGATGGAAGACATGGGTGTGATTGGGGGGCAGCTTGCCATGTACACCGTGACT; GTCATTGTTGGCTTACTCATTCACGCAGTCATCGTCTTGTCACTCCTCTACTTCTTGGTA; ACACGGAAAAACCCTTGGGTTTTTATTGGAGGGTTGCTGCAAGCACTCATCACCGCTCTG; GGGACCTCTTCAAGTTCTGCCACCCTACCCATCACCTTCAAGTGCCTGGAAGAGAACAAT; GGCGTGGACAAGCGCGTCACCAGATTCGTGCTCCCCGTAGGAGCCACCATTAACATGGAT; GGGACTGCCCTCTATGAGGCTTTGGCTGCCATTTTCATTGCTCAAGTTAACAACTTTGAA; CTGAACTTCGGACAAATTATTACAATCAGCATCACAGCCACAGCTGCCAGTATTGGGGCA; GCTGGAATTCCTCAGGCGGGCCTGGTCACTATGGTCATTGTGCTGACATCTGTCGGCCTG; CCCACTGACGACATCACGCTCATCATCGCGGTGGACTGGTTCCTGGATCGCCTCCGGACC; ACCACCAACGTACTGGGAGACTCCCTGGGAGCTGGGATTGTGGAGCACTTGTCACGACAT; GAACTGAAGAACAGAGATGTTGAAATGGGTAACTCAGTGATTGAAGAGAATGAAATGAAG; AAACCATATCAACTGATTGCACAGGACAATGAAACTGAGAAACCCATCGACAGTGAAACC; AAGATGTAG; ; Clone variation with respect to NM_004172.4; 1000 c=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004172 unedited; GGGTTCAAATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGGTCAGGGC; TAGCCTGCCTGCTTACGCGCGCTGCGGATTGTTGCTCCGTTGTACCTGCTGGGGAATTCA; CCTCGTTACTGCTTGATATCTTCCACCCCTTACAAAATCAGAAAAGTTGTGTTTTCTAAT; ACCAAAGAGGAGGTTTGGCTTTCTGTGGGTGATTCCCAGACACTGAAGTGCAAAGAAGAG; ACCCTCCTAGAAAAGTAAAATATGACTAAAAGCAATGGAGAAGAGCCCAAGATGGGGGGC; AGGATGGAGAGATTCCAGCAGGGAGTCCGTAAACGCACACTTTTGGCCAAGAAGAAAGTG; CAGAACATTACAAAGGAGGATGTTAAAAGTTACCTGTTTCGGAATGCTTTTGTGCTGCTC; ACAGTCACCGCTGTCATTGTGGGTACAATCCTTGGATTTACCCTCCGACCATACAGAATG; AGCTACCGGGAAGTCAAGTACTTCTCCTTTCCTGGGGAACTTCTGATGAGGATGTTACAG; ATGCTGGTCTTACCACTTATCATCTCCAGTCTTGTCACAGGAATGGCGGCGCTAGATAGT; AAGGCATCAGGGAAGATGGGAATGCGAGCTGTAGTCTATTATATGACTACCACCATCATT; GCTGTGGTGATTGGCATAATCATTGTCATCATCATCCATCCTGNGAAGGGCACAAAGGAA; AACATGCACAGAGAAGGCAAAATTGTACGAGTGACAGCTGCAGATGCCTTCCTGGACTTG; ATCAGGAACATGTTCCCTTNCANATCTGTAGAAGCCTGCTTTANACAGTTTAAAACCAAC; TATGAGAAGAGAAGCTNNTAAGTGCCCATCCAGACCAACGAAACGCTTGTGGGTGCTGTG; ATAAACAATGTGTCTGA;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004172 unedited; GCAACTTTCCAGGGCCAGGNAAAGCACTGGGGAGGGGTCACAGGGATGCCACCCGGGATC; TGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTT; TTTTAATGTAAAACTATTTTTTTCTTTATTGAAAATACATCTACAAAGTAATGCTTCCCA; GTCCACACTGAGTGCATGTGTATGCAAAGCCTGTGATGGCTTGGCGAGGGGATGGGCTCC; CCACAGGACCTTTCCATAAGAAATGGTTAGTATATTAAAAAACACATATTGTGTAACAGA; ATTAAGTTGATATGATTAAGTCTTTGCTCTACTAGTGTCTTAAGAGGACAGCCACGTACT; TTAACATTTGTAGTGTATTTCCGAAATAGAGCCTCGACATTAAATGGTTACTGCACCACA; GCCTAATGTTAACTTTTATGCACAGAATGCTTGGGGTCTATATAAAAATATCTCTAATTG; TATATCAGAGGATCTATGTCTAGATTTTTCAGTTTGTTAGCATAATTAAGTCCATTCTTC; ACCATTTTCAGCCAGAAACTCAAACACAGCTGCTACTGCCTGACAGCTCCCACTCAGCGT; CTTTGACTGGATATTCCTTANGGGGTAAAAATTAATCTCTAAACCTGGNGAGCTGCCTTG; CCTGAAACTAATGCCCNCCTGCTGTTTTTTTCTCATAAACCTCATTGACAGNNGNCTTCA; TACTGGGATTCATCTTCTACCATTTTCTCTTNATGGNNACACAAACANACANAAAGCAAC; CCCACTTCAGCNTGAAAGTTTTTGAATGATTTGGAATAAGTGGGGATTCCAAACAAATAA; AATATTTTGGTTTCCCTCATTCTAAGGGACCTTTGGATTAAAGAAAAACACTTTCNATAA; NGAATACCCACTCACTCCTGGGACTTCTTTAAAGAACTCATCTTTTGA;
• Restriction Sites: NotI-NotI
• ACCN: NM_004172
• Insert Size: 1629 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_004172.3, NP_004163.2
• RefSeq Size: 3983 bp
• RefSeq ORF: 1629 bp
• Locus ID: 6507
• UniProt ID: P43003
• Cytogenetics: 5p13.2
• Domains: SDF
• Protein Families: Transmembrane
• Summary: This gene encodes a member of a member of a high affinity glutamate transporter family. This gene functions in the termination of excitatory neurotransmission in central nervous system. Mutations are associated with episodic ataxia, Type 6. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Feb 2014]; Transcript Variant: This variant (GLAST) represents the longest transcript and encodes the longest protein (isoform 1).