Surb7 (MED21) (NM_004264) Human Untagged Clone

SKU SC117467

MED21 (untagged)-Human mediator complex subunit 21 (MED21)

Product Data

• Type: Human Untagged Clone
• Target Symbol: Surb7
• Synonyms: hSrb7; SRB7; SURB7
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_004264, the custom clone sequence may differ by one or more nucleotides; ; ; ATGGCGGATCGGCTCACGCAGCTTCAGGACGCTGTGAATTCGCTTGCAGATCAGTTTTGTAATGCCATTG; GAGTATTGCAGCAATGTGGTCCTCCTGCCTCTTTCAATAATATTCAGACAGCAATTAACAAAGACCAGCC; AGCTAACCCTACAGAAGAGTATGCCCAGCTTTTTGCAGCACTGATTGCACGAACAGCAAAAGACATTGAT; GTTTTGATAGATTCCTTACCCAGTGAAGAATCTACAGCTGCTTTACAGGCTGCTAGCTTGTATAAGCTAG; AAGAAGAAAACCATGAAGCTGCTACATGTCTGGAGGATGTTGTTTATCGAGGAGACATGCTTCTGGAGAA; GATACAAAGCGCACTTGCTGATATTGCACAGTCACAGCTGAAGACAAGAAGTGGTACCCATAGCCAGTCT; CTTCCAGACTCATAG; ;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004264 unedited; GTGATTAGAAACCGACTTTATATAGGCGGCCGCGCAATTCGCACGAGGAACATGGCGGAT; CGGCTCACGCAGCTTCAGGACGCTGTGAATTCGCTTGCAGATCAGTTTTGTAATGCCATT; GGAGTATTGCAGCAATGTGGTCCTCCTGCCTCTTTCAATAATATTCAGACAGCAATTAAC; AAAGACCAGCCAGCTAACCCTACAGAAGAGTATGCCCAGCTTTTTGCAGCACTGATTGCA; CGAACAGCAAAAGACATTGATGTTTTGATAGATTCCTTACCCAGTGAAGAATCTACAGCT; GCTTTACAGGCTGCTAGCTTGTATAAGCTAGAAGAAGAAAACCATGAAGCTGCTACATGT; CTGGAGGATGTTGTTTATCGAGGAGACATGCTTCTGGAGAAGATACAAAGCGCACTTGCT; GATATTGCACAGTCACAGCTGAAGACAAGAAGTGGTACCCATAGCCAGTCTCTTCCAGAC; TCATAGCATCAGTGGATACCATGTGGCTGAGAAAAGAACTGTTTGAGTGCCATTAAGAAT; TCTGCATCAGACTTAGATACAAGCCTTACCAACAATTACAGAAACATTAAACACTATGAC; ACATTACCTTTTTAGCTATTTTTAATAGTCTTCTATTTTCACTCTTGATAAGCTTATAAA; ATCATGATTGAAATCAGCTTTAAGCATCATACCATCATTTTTTAACTGAGTGGAATTATT; AAGGCATGNTATACATTAATGAACATAATATAAGGAAACATATGTAAAATNCTGTTATGA; CATAATTTATGTCTCCATTTTGTTGNATTGGCCAGTACTTTTACAAATCAAAACATCTCT; CAAGCCAAAGGAGAAGACAGTTAGAACAGACATAGGGGACATTNTAGTTTAAGCTAGTGT; CCTGCCTCTTAAAGTGGCATTGGGTAATTCTGAGCTTTGAGCACGAAAAGTTTGGAAATT; GTGTTGCTTTTAGAAAAAAAGTTAGTGGG;
• Restriction Sites: NotI-NotI
• ACCN: NM_004264
• Insert Size: 2770 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_004264.2, NP_004255.2
• RefSeq Size: 1783 bp
• RefSeq ORF: 435 bp
• Locus ID: 9412
• UniProt ID: Q13503
• Cytogenetics: 12p11.23
• Protein Families: Transcription Factors
• Summary: This gene encodes a member of the mediator complex subunit 21 family. The encoded protein interacts with the human RNA polymerase II holoenzyme and is involved in transcriptional regulation of RNA polymerase II transcribed genes. A pseudogene of this gene is located on chromosome 8. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2012]; Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.