TRAF4 (NM_004295) Human Untagged Clone

CAT#: SC117456

TRAF4 (untagged)-Human TNF receptor-associated factor 4 (TRAF4)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TRAF4
• Synonyms: CART1; MLN62; RNF83
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC117456 sequence for NM_004295 edited (data generated by NextGen Sequencing)
  ATGCCTGGCTTCGACTACAAGTTCCTGGAGAAGCCCAAGCGACGGCTGCTGTGCCCACTG
  TGCGGGAAGCCCATGCGCGAGCCTGTGCAGGTTTCCACCTGCGGCCACCGTTTCTGCGAT
  ACCTGCCTGCAGGAGTTCCTCAGTGAAGGAGTCTTCAAGTGCCCTGAGGACCAGCTTCCT
  CTGGACTATGCCAAGATCTACCCAGACCCGGAGCTGGAAGTACAAGTATTGGGCCTGCCT
  ATCCGCTGCATCCACAGTGAGGAGGGCTGCCGCTGGAGTGGGCCACTACGTCATCTACAG
  GGCCACCTGAATACCTGCAGCTTCAATGTCATTCCCTGCCCTAATCGCTGCCCCATGAAG
  CTGAGCCGCCGTGATCTACCTGCACACTTGCAGCATGACTGCCCCAAGCGGCGCCTCAAG
  TGCGAGTTTTGTGGCTGTGACTTCAGTGGGGAGGCCTATGAGAGCCATGAGGGTATGTGC
  CCCCAGGAGAGTGTCTACTGTGAGAATAAGTGTGGTGCCCGCATGATGCGGCGGCTGCTG
  GCCCAGCATGCCACCTCTGAGTGCCCCAAGCGCACTCAGCCCTGCACCTACTGCACTAAG
  GAGTTCGTCTTTGACACCATCCAGAGCCACCAGTACCAGTGCCCAAGGCTGCCTGTTGCC
  TGCCCCAACCAATGTGGTGTGGGCACTGTGGCTCGGGAGGACCTGCCAGGCCATCTGAAG
  GACAGCTGTAACACCGCCCTGGTGCTCTGCCCATTCAAAGACTCCGGCTGCAAGCACAGG
  TGCCCTAAGCTGGCAATGGCACGGCATGTGGAGGAGAGTGTGAAGCCACATCTGGCCATG
  ATGTGTGCCCTGGTGAGCCGGCAACGGCAGGAGCTGCAGGAGCTTCGGCGAGAGCTGGAG
  GAGCTATCAGTGGGCAGTGATGGCGTGCTCATCTGGAAGATTGGCAGCTATGGACGGCGG
  CTACAGGAGGCCAAGGCCAAGCCCAACCTTGAGTGCTTCAGCCCAGCCTTCTACACACAT
  AAGTATGGTTACAAGCTGCAGGTGTCTGCATTCCTCAATGGCAATGGCAGTGGTGAGGGC
  ACACACCTCTCACTGTACATTCGTGTGCTGCCTGGTGCCTTTGACAATCTCCTTGAGTGG
  CCCTTTGCCCGCCGTGTCACCTTCTCCCTGCTGGATCAGAGCGACCCTGGGCTGGCTAAA
  CCACAGCACGTCACTGAGACCTTCCACCCCGACCCAAACTGGAAGAATTTCCAGAAGCCA
  GGCACGTGGCGGGGCTCCCTGGATGAGAGTTCTCTGGGCTTTGGTTATCCCAAGTTCATC
  TCCCACCAGGACATTCGAAAGCGAAACTATGTGCGGGATGATGCAGTCTTCATCCGTGCT
  GCTGTTGAACTGCCCCGGAAGATCCTCAGCTGA
  
  Clone variation with respect to NM_004295.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004295 unedited
  TTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGCCGCCCGGAGCC
  GGGAGCGCCGCTCCAGCGAGGCGCGGGCTGTGGGGCCGCCGCGTGCCTGGCCCCGCTCGC
  CCGTGCCGGCCGCTCGCCCGCCATGCCTGGCTTCGACTACAAGTTCCTGGAGAAGCCCAA
  GCGACGGCTGCTGTGCCCACTGTGCGGGAAGCCCATGCGCGAGCCTGTGCAGGTTTCCAC
  CTGCGGCCACCGTTTCTGCGATACCTGCCTGCAGGAGTTCCTCAGTGAAGGAGTCTTCAA
  GTGCCCTGAGGACCAGCTTCCTCTGGACTATGCCAAGATCTACCCAGACCCGGAGCTGGA
  AGTACAAGTATTGGGCCTGCCTATCCGCTGCATCCACAGTGAGGAGGGCTGCCGCTGGAG
  TGGGCCACTACGTCATCTACAGGGCCACCTGAATACCTGCAGCTTCAATGTCATTCCCTG
  CCCTAATCGCTGCCCCATGAAGCTGAGCCGCCGTGATCTACCTGCACACTTGCAGCATGA
  CTGCCCCAAGCGGCGCCTCAAGTGCGAGTTTTGTGGCTGTGACTTCAGTGGGGAGGCCTA
  TGAGAGCCATGANGGTATGTGCCCCCAGGAGAGTGTCTACTGTGAGAATAAGTGTGGTGC
  CCGCATGATGCGGCGGCTGCTGGCCCAGCATGCCACCTCTGAGTGCCCCAAGCGCACTCA
  GCCCTGCACCTACTGCACTAAGGGAGTCGTCTTTGCACCATCCAGAGCCCCANTCCAGTG
  CCCAAGCTGCCTGTGCCTGCCCAACCAATGTGTGTGGCACTGTGGCTTCGGAGGACTGCC
  AGCCATCTGAAGACACTGTACACCGGCTGGGCTTTGCCATCAAAGACTCGCTGCAGACAG
  TGCC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004295 unedited
  GATCTATTAATTATCAGGGACGTCCCCTGGCTGTGNCNNCCTCCTGGCACTAAGGGAAAT
  AAATAACCAGAGGGGGCACAGCTAGGTAAAGGGGTGAAAAAATCAGATCTCAAGACAGAC
  TCTTTGAACTACCCTTTCTCCCTCCCCAATTCCCACCCCCTTATGGCTCTTGGGACATAG
  CACCTGAGCAGGCACCTCAGTCCGTGTAGAGTGTGACCGGCACCAAGGCATGTCTGCCCT
  ACCCAAGAAGGGAGACAGGCCCTGGGGTCACTTGTTTCCAGCCCTTCATGACCCCAAGAC
  CTGTTCCCCACAGGGGCCAGGGCCTGAAGCACCAATCGGAGGCACCTGATGGTGGTGGCC
  GGGGGAGGGAGGATGGGGTCACATAACCTAGGCAGCAAAAGCAGCTGACCGGGGTCCTGC
  CCCCTTTTCACCTTCATTTCCCGCCTGCCCGCCGCCATGCCCTCTTTCCTTTTTCCCCAC
  CCCAGCTGCCCGAGCATAGGATCTCCGGGGCGCTTCCCACTTCTCTCTTTCCCGCCTTGC
  TTCTTTGCCTCAGCTCTCCTATCTTCTGCCCCCTCTTCCCCTATTCGTTATCTCACCCTC
  CCCCCTCCTTTCCTATTCTACCCCCTCCCTTCCCCGCTCCCGTACCGCTCCGTTCCACTC
  GTCCTTCCATCGCTTCCTTTTTTCTCTCTTTTCCCATCCCTCACCCCTATCTCATTTTTG
  TTTTTCTTTCCACCTATCCTCCGCCCTCTCCTCCGTTTTACTCTTCCCCTCCGCCCTTCC
  TTTTCTCAGTTCACTCCCCCTTCCGCGTTCTCCCCCTTTGTTCCCCCTCTTTCTCCCTTC
  CCCCATCTTCTTCCTTTTCGCCCGATATCTCCCTTTTCTCCGATCCTCTTTATCCCCCCT
  CCTCTTCCACCTCTACTCTTCCTCTTTCATCTTCCGTCTCCCTCTTCTACTTCCCTCTTT
  TCCTCCTCCCCTTCTCCTTCTTTCCCCCCTCTTCCCCCTTT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_004295
• Insert Size: 2210 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004295.2, NP_004286.2
• RefSeq Size: 1999 bp
• RefSeq ORF: 1413 bp
• Locus ID: 9618
• UniProt ID: Q9BUZ4
• Cytogenetics: 17q11.2
• Domains: zf-TRAF, RING, MATH
• Protein Families: Druggable Genome
• Protein Pathways: Pathways in cancer, Small cell lung cancer
• Gene Summary: This gene encodes a member of the TNF receptor associated factor (TRAF) family. TRAF proteins are associated with, and mediate signal transduction from members of the TNF receptor superfamily. The encoded protein has been shown to interact with neurotrophin receptor, p75 (NTR/NTSR1), and negatively regulate NTR induced cell death and NF-kappa B activation. This protein has been found to bind to p47phox, a cytosolic regulatory factor included in a multi-protein complex known as NAD(P)H oxidase. This protein thus, is thought to be involved in the oxidative activation of MAPK8/JNK. Alternatively spliced transcript variants have been observed but the full-length nature of only one has been determined. [provided by RefSeq, Jul 2008]