TRAF4 (NM_004295) Human Untagged Clone

SKU SC117456

TRAF4 (untagged)-Human TNF receptor-associated factor 4 (TRAF4)

Product Data

• Type: Human Untagged Clone
• Target Symbol: TRAF4
• Synonyms: CART1; MLN62; RNF83
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC117456 sequence for NM_004295 edited (data generated by NextGen Sequencing); ATGCCTGGCTTCGACTACAAGTTCCTGGAGAAGCCCAAGCGACGGCTGCTGTGCCCACTG; TGCGGGAAGCCCATGCGCGAGCCTGTGCAGGTTTCCACCTGCGGCCACCGTTTCTGCGAT; ACCTGCCTGCAGGAGTTCCTCAGTGAAGGAGTCTTCAAGTGCCCTGAGGACCAGCTTCCT; CTGGACTATGCCAAGATCTACCCAGACCCGGAGCTGGAAGTACAAGTATTGGGCCTGCCT; ATCCGCTGCATCCACAGTGAGGAGGGCTGCCGCTGGAGTGGGCCACTACGTCATCTACAG; GGCCACCTGAATACCTGCAGCTTCAATGTCATTCCCTGCCCTAATCGCTGCCCCATGAAG; CTGAGCCGCCGTGATCTACCTGCACACTTGCAGCATGACTGCCCCAAGCGGCGCCTCAAG; TGCGAGTTTTGTGGCTGTGACTTCAGTGGGGAGGCCTATGAGAGCCATGAGGGTATGTGC; CCCCAGGAGAGTGTCTACTGTGAGAATAAGTGTGGTGCCCGCATGATGCGGCGGCTGCTG; GCCCAGCATGCCACCTCTGAGTGCCCCAAGCGCACTCAGCCCTGCACCTACTGCACTAAG; GAGTTCGTCTTTGACACCATCCAGAGCCACCAGTACCAGTGCCCAAGGCTGCCTGTTGCC; TGCCCCAACCAATGTGGTGTGGGCACTGTGGCTCGGGAGGACCTGCCAGGCCATCTGAAG; GACAGCTGTAACACCGCCCTGGTGCTCTGCCCATTCAAAGACTCCGGCTGCAAGCACAGG; TGCCCTAAGCTGGCAATGGCACGGCATGTGGAGGAGAGTGTGAAGCCACATCTGGCCATG; ATGTGTGCCCTGGTGAGCCGGCAACGGCAGGAGCTGCAGGAGCTTCGGCGAGAGCTGGAG; GAGCTATCAGTGGGCAGTGATGGCGTGCTCATCTGGAAGATTGGCAGCTATGGACGGCGG; CTACAGGAGGCCAAGGCCAAGCCCAACCTTGAGTGCTTCAGCCCAGCCTTCTACACACAT; AAGTATGGTTACAAGCTGCAGGTGTCTGCATTCCTCAATGGCAATGGCAGTGGTGAGGGC; ACACACCTCTCACTGTACATTCGTGTGCTGCCTGGTGCCTTTGACAATCTCCTTGAGTGG; CCCTTTGCCCGCCGTGTCACCTTCTCCCTGCTGGATCAGAGCGACCCTGGGCTGGCTAAA; CCACAGCACGTCACTGAGACCTTCCACCCCGACCCAAACTGGAAGAATTTCCAGAAGCCA; GGCACGTGGCGGGGCTCCCTGGATGAGAGTTCTCTGGGCTTTGGTTATCCCAAGTTCATC; TCCCACCAGGACATTCGAAAGCGAAACTATGTGCGGGATGATGCAGTCTTCATCCGTGCT; GCTGTTGAACTGCCCCGGAAGATCCTCAGCTGA; ; Clone variation with respect to NM_004295.3;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004295 unedited; TTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGCCGCCCGGAGCC; GGGAGCGCCGCTCCAGCGAGGCGCGGGCTGTGGGGCCGCCGCGTGCCTGGCCCCGCTCGC; CCGTGCCGGCCGCTCGCCCGCCATGCCTGGCTTCGACTACAAGTTCCTGGAGAAGCCCAA; GCGACGGCTGCTGTGCCCACTGTGCGGGAAGCCCATGCGCGAGCCTGTGCAGGTTTCCAC; CTGCGGCCACCGTTTCTGCGATACCTGCCTGCAGGAGTTCCTCAGTGAAGGAGTCTTCAA; GTGCCCTGAGGACCAGCTTCCTCTGGACTATGCCAAGATCTACCCAGACCCGGAGCTGGA; AGTACAAGTATTGGGCCTGCCTATCCGCTGCATCCACAGTGAGGAGGGCTGCCGCTGGAG; TGGGCCACTACGTCATCTACAGGGCCACCTGAATACCTGCAGCTTCAATGTCATTCCCTG; CCCTAATCGCTGCCCCATGAAGCTGAGCCGCCGTGATCTACCTGCACACTTGCAGCATGA; CTGCCCCAAGCGGCGCCTCAAGTGCGAGTTTTGTGGCTGTGACTTCAGTGGGGAGGCCTA; TGAGAGCCATGANGGTATGTGCCCCCAGGAGAGTGTCTACTGTGAGAATAAGTGTGGTGC; CCGCATGATGCGGCGGCTGCTGGCCCAGCATGCCACCTCTGAGTGCCCCAAGCGCACTCA; GCCCTGCACCTACTGCACTAAGGGAGTCGTCTTTGCACCATCCAGAGCCCCANTCCAGTG; CCCAAGCTGCCTGTGCCTGCCCAACCAATGTGTGTGGCACTGTGGCTTCGGAGGACTGCC; AGCCATCTGAAGACACTGTACACCGGCTGGGCTTTGCCATCAAAGACTCGCTGCAGACAG; TGCC;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004295 unedited; GATCTATTAATTATCAGGGACGTCCCCTGGCTGTGNCNNCCTCCTGGCACTAAGGGAAAT; AAATAACCAGAGGGGGCACAGCTAGGTAAAGGGGTGAAAAAATCAGATCTCAAGACAGAC; TCTTTGAACTACCCTTTCTCCCTCCCCAATTCCCACCCCCTTATGGCTCTTGGGACATAG; CACCTGAGCAGGCACCTCAGTCCGTGTAGAGTGTGACCGGCACCAAGGCATGTCTGCCCT; ACCCAAGAAGGGAGACAGGCCCTGGGGTCACTTGTTTCCAGCCCTTCATGACCCCAAGAC; CTGTTCCCCACAGGGGCCAGGGCCTGAAGCACCAATCGGAGGCACCTGATGGTGGTGGCC; GGGGGAGGGAGGATGGGGTCACATAACCTAGGCAGCAAAAGCAGCTGACCGGGGTCCTGC; CCCCTTTTCACCTTCATTTCCCGCCTGCCCGCCGCCATGCCCTCTTTCCTTTTTCCCCAC; CCCAGCTGCCCGAGCATAGGATCTCCGGGGCGCTTCCCACTTCTCTCTTTCCCGCCTTGC; TTCTTTGCCTCAGCTCTCCTATCTTCTGCCCCCTCTTCCCCTATTCGTTATCTCACCCTC; CCCCCTCCTTTCCTATTCTACCCCCTCCCTTCCCCGCTCCCGTACCGCTCCGTTCCACTC; GTCCTTCCATCGCTTCCTTTTTTCTCTCTTTTCCCATCCCTCACCCCTATCTCATTTTTG; TTTTTCTTTCCACCTATCCTCCGCCCTCTCCTCCGTTTTACTCTTCCCCTCCGCCCTTCC; TTTTCTCAGTTCACTCCCCCTTCCGCGTTCTCCCCCTTTGTTCCCCCTCTTTCTCCCTTC; CCCCATCTTCTTCCTTTTCGCCCGATATCTCCCTTTTCTCCGATCCTCTTTATCCCCCCT; CCTCTTCCACCTCTACTCTTCCTCTTTCATCTTCCGTCTCCCTCTTCTACTTCCCTCTTT; TCCTCCTCCCCTTCTCCTTCTTTCCCCCCTCTTCCCCCTTT;
• Restriction Sites: NotI-NotI
• ACCN: NM_004295
• Insert Size: 2210 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_004295.2, NP_004286.2
• RefSeq Size: 1999 bp
• RefSeq ORF: 1413 bp
• Locus ID: 9618
• UniProt ID: Q9BUZ4
• Cytogenetics: 17q11.2
• Domains: MATH, RING, zf-TRAF
• Protein Families: Druggable Genome
• Protein Pathways: Pathways in cancer, Small cell lung cancer
• Summary: This gene encodes a member of the TNF receptor associated factor (TRAF) family. TRAF proteins are associated with, and mediate signal transduction from members of the TNF receptor superfamily. The encoded protein has been shown to interact with neurotrophin receptor, p75 (NTR/NTSR1), and negatively regulate NTR induced cell death and NF-kappa B activation. This protein has been found to bind to p47phox, a cytosolic regulatory factor included in a multi-protein complex known as NAD(P)H oxidase. This protein thus, is thought to be involved in the oxidative activation of MAPK8/JNK. Alternatively spliced transcript variants have been observed but the full-length nature of only one has been determined. [provided by RefSeq, Jul 2008]