Glypican 5 (GPC5) (NM_004466) Human Untagged Clone

SKU SC117378

GPC5 (untagged)-Human glypican 5 (GPC5)

Product Data

• Type: Human Untagged Clone
• Target Symbol: Glypican 5
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC117378 sequence for NM_004466 edited (data generated by NextGen Sequencing); ATGGACGCACAGACCTGGCCCGTGGGCTTTCGCTGCCTCCTCCTTCTGGCCCTGGTTGGG; TCCGCCCGCAGCGAGGGCGTGCAGACCTGCGAAGAAGTTCGGAAACTTTTCCAGTGGCGG; CTGCTGGGAGCTGTCAGGGGGCTGCCGGATTCGCCGCGGGCAGGACCTGATCTTCAGGTT; TGCATATCCAAAAAGCCTACATGTTGCACCAGGAAGATGGAGGAGAGATATCAGATTGCG; GCTCGCCAGGATATGCAGCAGTTTCTTCAAACGTCCAGCTCTACATTAAAGTTTCTAATA; TCTCGAAATGCGGCTGCTTTTCAAGAAACCCTTGAAACTCTCATCAAACAAGCAGAAAAT; TACACCAGTATACTTTTTTGCAGTACCTACAGGAACATGGCCTTGGAGGCTGCTGCTTCG; GTTCAGGAGTTCTTCACTGATGTGGGGCTGTATTTATTTGGTGCGGATGTTAATCCTGAA; GAATTTGTAAACAGATTTTTTGACAGTCTTTTTCCTCTGGTCTACAACCACCTCATTAAC; CCTGGTGTGACTGACAGTTCCCTGGAATACTCAGAATGCATCCGGATGGCTCGCCGGGAT; GTGAGTCCATTTGGTAATATTCCCCAAAGAGTAATGGGACAGATGGGGAGGTCCCTGCTG; CCCAGCCGCACTTTTCTGCAGGCACTCAATCTGGGCATTGAAGTCATCAACACCACAGAC; TATCTGCACTTCTCCAAAGAGTGCAGCAGAGCCCTCCTGAAGATGCAATACTGCCCGCAC; TGCCAAGGCCTGGCGCTCACTAAGCCTTGTATGGGATACTGCCTCAATGTCATGCGAGGC; TGCCTGGCGCACATGGCGGAGCTTAATCCACACTGGCATGCATATATCCGGTCGTTGGAA; GAACTCTCGGATGCAATGCATGGAACATACGACATTGGACACGTGCTGCTGAACTTTCAC; TTGCTTGTTAATGATGCTGTGTTACAGGCTCACCTCAATGGACAAAAATTATTGGAACAG; GTAAATAGGATTTGTGGCCGCCCTGTAAGAACACCCACACAAAGCCCCCGTTGTTCTTTT; GATCAGAGCAAAGAGAAGCATGGAATGAAGACCACCACAAGGAACAGTGAAGAGACGCTT; GCCAACAGAAGAAAAGAATTTATCAACAGCCTTCGACTGTACAGGTCATTCTATGGAGGT; CTAGCTGATCAGCTTTGTGCTAATGAATTAGCTGCTGCAGATGGACTTCCCTGCTGGAAT; GGAGAAGATATAGTAAAAAGTTATACTCAGCGTGTGGTTGGAAATGGAATCAAAGCCCAG; TCTGGAAATCCTGAAGTCAAAGTCAAAGGAATTGATCCTGTGATAAATCAGATTATTGAT; AAACTGAAGCATGTTGTTCAGTTGTTACAGGGTAGATCACCCAAACCTGACAAGTGGGAA; CTTCTTCAGCTGGGCAGTGGTGGAGGCATGGTTGAACAAGTCAGTGGGGACTGTGATGAT; GAAGATGGTTGCGGGGGATCAGGAAGTGGAGAAGTCAAGAGGACACTGAAGATCACAGAC; TGGATGCCAGATGATATGAACTTCAGTGATGTAAAGCAAATCCATCAAACAGACACTGGC; AGTACTTTAGACACAACAGGAGCAGGATGTGCAGTGGCGACTGAATCTATGACATTCACT; CTGATAAGTGTGGTGATGTTACTTCCCGGGATTTGGTAA; ; Clone variation with respect to NM_004466.4;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004466 unedited; GTCCCCATTTGTATACGACTCATATAGGGCGGCCGCGAATTCGCACGAGGCGTACACCCC; GCAGCCGGCTCGCACCGCTCGAGAGCCTCGGCCGCTGTGTCTTCCACGTCTGCAGCTCAG; CCAGGGCGCGCAGGGCGAGTGGGGTCCACTGGCGGGTAAAGGGGACCAGGACGGCGAGGA; TGGACGCACAGACCTGGCCCGTGGGCTTTCGCTGCCTCCTCCTTCTGGCCCTGGTTGGGT; CCGCCCGCAGCGAGGGCGTGCAGACCTGCGAAGAAGTTCGGAAACTTTTCCAGTGGCGGC; TGCTGGGAGCTGTCAGGGGGCTGCCGGATTCGCCGCGGGCAGGACCTGATCTTCAGGTTT; GCATATCCAAAAAGCCTACATGTTGCACCAGGAAGATGGAGGAGAGATATCAGATTGCGG; CTCGCCAGGATATGCAGCAGTTTCTTCAAACGTCCAGCTCTACATTAAAGTTTCTAATAT; CTCGAAATGCGGCTGCTTTTCAAGAAACCCTTGAAACTCTCATCAAACAAGCCGAAAATT; ACACCAGTATACTTTTTTGCAGTACCTACAGGAACATGGCCTTGGAGGCTGCTGCTTCGG; TTCAGGAGTTCTTCACTGATGTGGGGCTGTATTTATTTGGTGCGGATGTTAATCCTGAAG; ATTTGNTAACAGATTTTTTGACAGTCTTTTTCCTCTGGTCTACAACCACCTCATTAACCC; TGGTGTGACTGACAGTTCCCTGGAATACTCANAATGCATNCGGATGGCTCGCCGGGATGT; GAGTCCATTTTGTAATATTCCCCAAAGAGTATGGGACAGAGGGGNAGTCCCTGCTGCCCA; GCCCACTTTTCTGCAGGACTCAATCTGGGCATTGAATCATCACACCACAGACTTCTGCAC; TCTTCAAGATGCAGCAGAGCCCTCTGAGATGCATACATGCG;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004466 unedited; TTCTTGGATATATCTATGNNACCGCGGCCGCATNCTANGATCGAGTTTTTTTTTTTTTTT; TTTTTTTTTTTTTTTTTTTTTTTTTTTCCACCAAATAGTTAACAGCTTTATTGGAAATAT; TCCTAGAGCAAGCATGTGCTTCCCAACATAGCTAAATCTATGAGGCAAAAAGAGAGATGG; GAGCAAAGGCCCAATACCAAAGATGCATTTTGAATATAAGGGAATACAAATCAGGGGTTA; ATAAAATACTCAGCTTTAAGGTTCATCAGGGGCTAAGTTACAGGGGGTCAAAATTATCAA; GGTTCTTCAGGGAAATTATATGAAATGCTTCTCAAAAATCTACCCCATAACTTATAGACA; TTCAAACATTTCCTTCTACAGCTTGCAAAAATGGAAAAGTCTCTTGAATATGCATGCATA; CTACTTGCCATTGGATGTTTTGATTTATTTCATGATATTCTAATTTCAATACTTTAAAAA; ATACTGCATACTGTTTTCATTGGCTGGCAAATACACTTTCAAATGAAAACATTTTTAATT; TAAAAAGGAAGGGGCTTGGAATTGATAATAAAATTACAGATCCCCTAAATTCTTCTTATT; CAAAGTCACTTCTGTGCCCTTCACATCTTCAGATTTAATATATTAAAAGTTAAGAAGGTT; TTCAGTTAGATTGAAAAAAGACATATTTTTAAAGTGTGTCATTTAAACGTTGAGTTTTAG; GGACTTTATGAATATTTCAGCTTGGCTTCTGAAAAGTTAGTGTAACATATCTTTTCATAA; AATAATTGTTACATTGAAAAAGATCTCTTTATCCAGGCCTATGCAAAAAGGGAAGAAATC; GAGACTTTGTTAGGTATTTTCAGGACAGAAAAATTCATA;
• Restriction Sites: ECoRI-NOT
• ACCN: NM_004466
• Insert Size: 2500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_004466.3, NP_004457.1
• RefSeq Size: 2934 bp
• RefSeq ORF: 1719 bp
• Locus ID: 2262
• UniProt ID: P78333
• Cytogenetics: 13q31.3
• Domains: Glypican
• Summary: Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation. [provided by RefSeq, Jul 2008]