NFAT4 (NFATC3) (NM_004555) Human Untagged Clone

SKU SC117277

NFATC3 (untagged)-Human nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 3 (NFATC3), transcript variant 2

Product Data

• Type: Human Untagged Clone
• Target Symbol: NFAT4
• Synonyms: NF-AT4c; NFAT4; NFATX
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_004555, the custom clone sequence may differ by one or more nucleotides; ; ; ATGACTACTGCAAACTGTGGCGCCCACGACGAGCTCGACTTCAAACTCGTCTTTGGCGAGGACGGGGCGC; CGGCGCCGCCGCCCCCGGGCTCGCGGCCTGCAGATCTTGAGCCAGATGATTGTGCATCCATTTACATCTT; TAATGTAGATCCACCTCCATCTACTTTAACCACACCACTTTGCTTACCACATCATGGATTACCGTCTCAC; TCTTCTGTTTTGTCACCATCGTTTCAGCTCCAAAGTCACAAAAACTATGAAGGAACTTGTGAGATTCCTG; AATCTAAATATAGCCCATTAGGTGGTCCCAAACCCTTTGAGTGCCCAAGTATTCAAATTACATCTATCTC; TCCTAACTGTCATCAAGAATTAGATGCACATGAAGATGACCTACAGATAAATGACCCAGAACGGGAATTT; TTGGAAAGGCCTTCTAGAGATCATCTCTATCTTCCTCTTGAGCCATCCTACCGGGAGTCTTCTCTTAGTC; CTAGTCCTGCCAGCAGCATCTCTTCTAGGAGTTGGTTCTCTGATGCATCTTCTTGTGAATCGCTTTCACA; TATTTATGATGATGTGGACTCAGAGTTGAATGAAGCTGCAGCCCGATTTACCCTTGGATCCCCTCTGACT; TCTCCTGGTGGCTCTCCAGGGGGCTGCCCTGGAGAAGAAACTTGGCATCAACAGTATGGACTTGGACACT; CATTATCACCCAGGCAATCTCCTTGCCACTCTCCTAGATCCAGTGTCACTGATGAGAATTGGCTGAGCCC; CAGGCCAGCCTCAGGACCCTCATCAAGGCCCACATCCCCCTGTGGGAAACGGAGGCACTCCAGTGCTGAA; GTTTGTTATGCTGGGTCCCTTTCACCCCATCACTCACCTGTTCCTTCACCTGGTCACTCCCCCAGGGGAA; GTGTGACAGAAGATACGTGGCTCAATGCTTCTGTCCATGGTGGGTCAGGCCTTGGCCCTGCAGTTTTTCC; ATTTCAGTACTGTGTAGAGACTGACATCCCTCTCAAAACAAGGAAAACTTCTGAAGATCAAGCTGCCATA; CTACCAGGAAAATTAGAGCTGTGTTCAGATGACCAAGGGAGTTTATCACCAGCCCGGGAGACTTCAATAG; ATGATGGCCTTGGATCTCAGTATCCTTTAAAGAAAGATTCATGTGGTGATCAGTTTCTTTCAGTTCCTTC; ACCCTTTACCTGGAGCAAACCAAAGCCTGGCCACACCCCTATATTTCGCACATCTTCATTACCTCCACTA; GACTGGCCTTTACCAGCTCATTTTGGACAATGTGAACTGAAAATAGAAGTGCAACCTAAAACTCATCATC; GAGCCCATTATGAAACTGAAGGTAGCCGAGGGGCAGTAAAAGCATCTACTGGGGGACATCCTGTTGTGAA; GCTCCTGGGCTATAACGAAAAGCCAATAAATCTACAAATGTTTATTGGGACAGCAGATGATCGATATTTA; CGACCTCATGCATTTTACCAGGTGCATCGAATCACTGGGAAGACAGTCGCTACTGCAAGCCAAGAGATAA; TAATTGCCAGTACAAAAGTTCTGGAAATTCCACTTCTTCCTGAAAATAATATGTCAGCCAGTATTGATTG; TGCAGGTATTTTGAAACTCCGCAATTCAGATATAGAACTTCGAAAAGGAGAAACTGATATTGGCAGAAAG; AATACTAGAGTACGACTTGTGTTTCGTGTACACATCCCACAGCCCAGTGGAAAAGTCCTTTCTCTGCAGA; TAGCCTCTATACCCGTTGAGTGCTCCCAGCGGTCTGCTCAAGAACTTCCTCATATTGAGAAGTACAGTAT; CAACAGTTGTTCTGTAAATGGAGGTCATGAAATGGTTGTGACTGGATCTAATTTTCTTCCAGAATCCAAA; ATCATTTTTCTTGAAAAAGGACAAGATGGACGACCTCAGTGGGAGGTAGAAGGGAAGATAATCAGGGAAA; AATGTCAAGGGGCTCACATTGTCCTTGAAGTTCCTCCATATCATAACCCAGCAGTTACAGCTGCAGTGCA; GGTGCACTTTTATCTTTGCAATGGCAAGAGGAAAAAAAGCCAGTCTCAACGTTTTACTTATACACCAGTT; TTGATGAAGCAAGAACACAGAGAAGAGATTGATTTGTCTTCAGTTCCATCTTTGCCTGTGCCTCATCCTG; CTCAGACCCAGAGGCCTTCCTCTGATTCAGGGTGTTCACATGACAGTGTACTGTCAGGACAGAGAAGTTT; GATTTGCTCCATCCCACAAACATATGCATCCATGGTGACCTCATCCCATCTGCCACAGTTGCAGTGTAGA; GATGAGAGTGTTAGTAAAGAACAGCATATGATTCCTTCTCCAATTGTACACCAGCCTTTTCAAGTCACAC; CAACACCTCCTGTGGGGTCTTCCTATCAGCCTATGCAAACTAATGTTGTGTACAATGGACCAACTTGTCT; TCCTATTAATGCTGCCTCTAGTCAAGAATTTGATTCAGTTTTGTTTCAGCAGGATGCAACTCTTTCTGGT; TTAGTGAATCTTGGCTGTCAACCACTGTCATCCATACCATTTCATTCTTCAAATTCAGGCTCAACAGGAC; ATCTCTTAGCCCATACACCTCATTCTGTGCATACCCTGCCTCATCTGCAATCAATGGGATATCATTGTTC; AAATACAGGACAAAGATCTCTTTCTTCTCCAGTGGCTGACCAGATTACAGGTCAGCCTTCGTCTCAGTTA; CAACCTATTACATATGGTCCTTCACATTCAGGGTCTGCTACAACAGCTTCCCCAGCAGCTTCTCATCCCT; TGGCTAGTTCACCGCTTTCTGGGCCACCATCTCCTCAGCTTCAGCCTATGCCTTACCAATCTCCTAGCTC; AGGAACTGCCTCATCACCGTCTCCAGCCACCAGAATGCATTCTGGACAGCACTCAACTCAAGCACAAAGT; ACGGGCCAGGGGGGTCTTTCTGCACCTTCATCCTTAATATGTCACAGTTTGTGTGATCCAGCGTCATTTC; CACCTGATGGGGCAACTGTGAGCATTAAACCTGAACCAGAAGATCGAGAGCCTAACTTTGCAACCATTGG; TCTGCAGGACATCACTTTAGATGATGACCAATTTATATCTGACTTGGAACACCAGCCATCAGGTTCAGCA; GAGAAATGGCCTAACCACAGTGTGCTCTCATGTCCAGCTCCTTTCTGGAGAATCTAG; ;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004555 unedited; NTGTCGNNATATTTGTATACGACTCACTATAGGGCGGCNCGCGAATTCGCACCAGGCGGG; CGGCTGCGGTTCCTGGTGCTGCTCGGCGCGCGGCCAGCTTTCGGAACGGAACGCTCGGCG; TCGCGGGCCCCGCCCGGAAAGTTTGCCGTGGAGTCGCGACCTCTTGGCCCGCGCGGCCCG; GCATGAAGCGGCGTTGAGGAGCTGCTGCCGCCGCTTGCCGCTGCCGCCGCCGCCGCCTGA; GGAGGAGCTGCAGCACCCTGGGCCACGCCGATGACTACTGCAAACTGTGGCGCCCACGAC; GAGCTCGACTTCAAACTCGTCTTTGGCGAGGACGGGGCGCCGGCGCCGCCGCCCCCGGGC; TCGCGGCCTGCAGATCTTGAGCCAGATGATTGTGCATCCATTTACATCTTTAATGTAGAT; CCACCTCCATCTACTTTAACCACACCACTTTGCTTACCACATCATGGATTACCGTCTCAC; TCTTCTGTTTTGTCACCATCGTTTCAGCTCCAAAGTCACAAAAACTATGAAGGAACTTGT; GAGATTCCTGAATCTAAATATAGCCCATTAGGTGGTCCCAAACCCTTTGAGTGCCCAAGT; ATTCAAATTACATCTATCTCTCCTAACTGTCATCAAGAATTAGATGCACATGAAGATGAC; CTACAGATAAATGACCCAGAACGGGAATTTTTGGAAAGGCCTTCTAGAGATCATCTCTAT; CTTCCTCTTGAGCCATCCTACCGGGAGTCTTCTCTTAGTCCTAGTCCTGCCAGCAGCATC; TCTTCTAGGAGTTGGNTCTCTGATGCATCTTCTTGTGAATCGCTTTCACATATTTATGAT; GATGTGGACTCAGAGTTGAATGAAGCTGCAGCCCGATTTACCCTTGATCCCCTCTGACTT; CTCTGGGGNCTCCTCGGGGGCTGCCCTGGAGAGAAACTTGCATCACA;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004555 unedited; CTATGAACCGCGGCACGCAATCTAGTATCGAGTNCTTTTTTTTTTTTTTTTTTTTTTTTT; TTTTTTTTTTTTTTTTTTTTTTTTTGGGCTCTAAAAGGCTTATAAAAAGGTCCTTTTTTA; AAAATTATCCTAAAAACCAAAGGGGCCCTTAAATGACCCTTAAGGAGTTCAAATTACCAA; TTCTTTCTTAATAACCAGGGCACCTGGAAAAGCAGCACCCCGGAAACAAAAAAACCTGGT; GGATTTACACTCAAAGGACCCCTCATATAAAAGGATCCCAATTAAATATAAAAAAGAAAG; TTTATTTCCCCAAAGCTTAAAAAGCCTATGCTGGTCCTGGTTTTTTGCCAAAATTACCCA; AGGGCCGAGGGGGGGGAATGGTTCTTTTCCCCACAAATGGGGGGGTCCCCTCCTGGGCCC; CCAACTTAAGGGTTGAAAATCTGAAAACCTAAGCGCAACGTAATAAACACTGTCGGAAAA; TTTGGTGGGGGGACTAAAGGCGCAGTACAACCCCTTTTAAGCCCCCTACATTTTTTCTTT; TTTATTGGTCATATTACTCTCTTCCGCGATCCTCTTCTCTCTTCCCATTTCCTAATCACC; CACATTTACCACCACCCCTTCTACCAATAATCATTCCTCACACCTCCTTCTCACATCGTC; CATCTTCTATTTGGCTAACCACCTGTTGTCTCGACCTCATACTTCCGTTTTACCTTACCA; ACTTCCTCCTATTCTATCTTCTGGCCAACACTTATCTCACCTACTGCGCCACCCCCCCCA; GCCCCATACCTAACCATTTATATATAGACAACCTCTCCATCTTTCTATCTTATCGCCTGC; TTCACTTGCCACCCCGACCATATGTTTTCTACTCTATTCCTCCCGTGTAGTTACACTCCC; GCCAAGTCTTCTCTCTATAACCCGCCTCGTTCATCCTATTCCACTTCTAACACCAGTACC; ACCCCTCTTTTCATTCACATTTATGTCTTCTGTATTCCACACCACGCCC;
• Restriction Sites: NotI-NotI
• ACCN: NM_004555
• Insert Size: 4000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_004555.2, NP_004546.1
• RefSeq Size: 4005 bp
• RefSeq ORF: 3207 bp
• Locus ID: 4775
• UniProt ID: Q12968
• Cytogenetics: 16q22.1
• Domains: IPT, RHD
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Axon guidance, B cell receptor signaling pathway, Natural killer cell mediated cytotoxicity, T cell receptor signaling pathway, VEGF signaling pathway, Wnt signaling pathway
• Summary: The product of this gene is a member of the nuclear factors of activated T cells DNA-binding transcription complex. This complex consists of at least two components: a preexisting cytosolic component that translocates to the nucleus upon T cell receptor (TCR) stimulation and an inducible nuclear component. Other members of this family participate to form this complex also. The product of this gene plays a role in the regulation of gene expression in T cells and immature thymocytes. Several transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Nov 2010]; Transcript Variant: This variant (2) contains an additional segment in the coding region, which causes a frameshift, compared to variant 1. The resulting isoform (2) contains a shorter and distinct C-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.