NDUFS6 (NM_004553) Human Untagged Clone

SKU SC117274

NDUFS6 (untagged)-Human NADH dehydrogenase (ubiquinone) Fe-S protein 6, 13kDa (NADH-coenzyme Q reductase) (NDUFS6), nuclear gene encoding mitochondrial protein

Product Data

• Type: Human Untagged Clone
• Target Symbol: NDUFS6
• Synonyms: CI-13kA; CI-13kD-A; CI13KDA; MC1DN9
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_004553 edited; ATGGCGGCGGCGATGACCTTCTGCCGGCTGCTGAACCGGTGTGGCGAGGCGGCGCGGAGC; CTGCCCCTGGGCGCCAGGTGTTTCGGGGTGCGGGTCTCGCCGACCGGGGAGAAGGTCACG; CACACTGGCCAGGTTTATGATGATAAAGACTACAGGAGAATTCGGTTTGTAGGTCGTCAG; AAAGAGGTGAATGAAAACTTTGCCATTGATTTGATAGCAGAGCAGCCCGTGAGCGAGGTG; GAGACTCGGGTGATAGCGTGCGATGGCGGCGGGGGAGCTCTTGGCCACCCAAAAGTGTAT; ATAAACTTGGACAAAGAAACAAAAACCGGCACATGCGGTTACTGTGGGCTCCAGTTCAGA; CAGCACCACCACTAG;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004553 unedited; ATTTATGTATACGACTTCCTATAGGGCGGCCGCGAATTCGGCACGAGGCCAGCGGCGCAA; AAGGCGGCGGCGATGACCTTCTGCCGGCTGCTGAACCGGTGTGGCGAGGCGGCGCGGAGC; CTGCCCCTGGGCGCCAGGTGTTTCGGGGTGCGGGTCTCGCCGACCGGGGAGAAGGTCACG; CACACTGGCCAGGTTTATGATGATAAAGACTACAGGAGAATTCGGTTTGTAGGTCGTCAG; AAAGAGGTGAATGAAAACTTTGCCATTGATTTGATAGCAGAGCAGCCCGTGAGCGAGGTG; GAGACTCGGGTGATAGCGTGCGATGGCGGCGGGGGAGCTCTTGGCCACCCAAAAGTGTAT; ATAAACTTGGACAAAGAAACAAAAACCGGCACATGCGGTTACTGTGGGCTCCAGTTCAGA; CAGCACCACCACTAGAGCGTGTGGCACGCCGGGGGTCCCGCAGCATCCTGTGAGCATTTC; CGCGGGGAAGCTGAGCACGTGAAGCTCGCTGGTTCTGTGCGAAGGGTATTCCTGGTGCTG; AATAAAGGGTGTTGCTGTCAAGGCTGAAAAAACAACCACCCCCCCCCCACCCAACACCAA; AACCCCACCCATAACCCCAACAAAAAAACTCGACTCTAGATTGCGGCCGCGGTCATAGTT; GTTTCCTGAACAGATCCCGGGTGGCATCCCTGTGACCCCTCCCCAGTGCCTTTCTTGCCC; CTGGAAGTTGCCTTCCAGGGCCCCCAGCCTTGGCCTATTAAAATAAGTGGCATCATTTTG; CCTGACTAGGTGTCCTTCTATATTTTATGGGGTGAGGCGGGGGGTGATGGGTCAAGGGCC; CCCTTTTTCCCCCTCCTTTCGTCTTCGGGGATCTTGCCCACCCATCCCCCTTTCTACCCC; CCCTGTTTNTCTTCACACGCCATCTCGTGTCCCAGTCCCCTTTTCCCTTCCCCTCCCACA; CCTAATTTTCCTCCCCTCCN;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004553 unedited; NCTCTTGNACGCGGCCGCATTCTANGATCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTT; TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAACCCTTGCAAGAAACCCCTTTTTTT; AAACCCCAGAAATCCCTTTCGAAAAAAACCAGGGAGTTTAAGGGGCTAATTTTCCCCGGG; AAAAGGCTAAAAGGATGCTGGGGAACCCCGGGGGGGCCAAACCCTTTAGGGGGGGGGCTG; TTTAAACGGGACCCCAAAAAACCCGGTGGGGCCGGTTTTTGTTTTTTTGCCCAAGTTAAT; ATACCCTTTGGGGGGGCAAAAAGCTCCCCCGCCGCCTTGGAACGTTTTAACCCGAGTTTC; CACCTTGTTAAGGGGTTGTTTTGTTTTAAAATAAAGGGCAAAGTTTTATTTCACCTTTTT; TTGACAACCTAAAAACCGAATTTTCCGGGAGTTTTTATAATAAAAAACCGGGCCAGGGGG; GGGGACCTTTTCCCCGGGGGGAAAAACCCGCACCCCAAAACACCTGGCGCCCAGGGGCAG; GTTCCGGGCCGCTTTGCCACACGGGTTAAAAAGCCGGAAAAAGGTCATCGCCGCCGCCAT; TTTGCGCCGTTGCCCTCGGGCCAAATTCGGGGCCGCCCTATAGGGAGTCGTATTACAAAA; TTTTGACGGGTCACTTAAACGAGCTCTGCTTAATAGACCTTCCACCGACCCGCCTACCGN; CAATTTGGGTCACCGGGGCGGGGTTATTACCACATTTTAGAAAAGCCCGGTTGATTTTTG; GGGCAAAACAAACTCCCTATGACGTAAGGGGGGGGGAAACCTGAAAATCCCCGGGGATAA; AACCCGTTCCACGCCCATTGGGGGCTGCCAAAACCGTTACCCTGGTAAAAGCGAGACTAA; TCCTANATTTCTGGCAAGNAGAAAATCCCCTAGGTATGACTGGGCATATGCCAA;
• Restriction Sites: NotI-NotI
• ACCN: NM_004553
• Insert Size: 630 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_004553.2, NP_004544.1
• RefSeq Size: 750 bp
• RefSeq ORF: 375 bp
• Locus ID: 4726
• UniProt ID: O75380
• Cytogenetics: 5p15.33
• Protein Pathways: Alzheimer's disease, Huntington's disease, Metabolic pathways, Oxidative phosphorylation, Parkinson's disease
• Summary: This gene encodes a subunit of the NADH:ubiquinone oxidoreductase (complex I), which is the first enzyme complex in the electron transport chain of mitochondria. This complex functions in the transfer of electrons from NADH to the respiratory chain. The subunit encoded by this gene is one of seven subunits in the iron-sulfur protein fraction. Mutations in this gene cause mitochondrial complex I deficiency, a disease that causes a wide variety of clinical disorders, including neonatal disease and adult-onset neurodegenerative disorders.[provided by RefSeq, Oct 2009]