EDG4 (LPAR2) (NM_004720) Human Untagged Clone

SKU SC117226

LPAR2 (untagged)-Human lysophosphatidic acid receptor 2 (LPAR2)

Product Data

• Type: Human Untagged Clone
• Target Symbol: EDG4
• Synonyms: EDG-4; EDG4; LPA-2; LPA2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_004720 edited; GAATTCGGCACGAGGGCAACCCGCACAGGGCGCACCCGGACGCTCTACCGCTCCCGCCGC; AGTCGCCGGGCCATGGGCCTCGAGCCCGCCCCGAACCCCCGCGAGCCCGCCTTGTCTGCG; GCGTGACTGGAGGCCCAGATGGTCATCATGGGCCAGTGCTACTACAACGAGACCATCGGC; TTCTTCTATAACAACAGTGGCAAAGAGCTCAGCTCCCACTGGCGGCCCAAGGATGTGGTC; GTGGTGGCACTGGGGCTGACCGTCAGCGTGCTGGTGCTGCTGACCAATCTGCTGGTCATA; GCAGCCATCGCCTCCAACCGCCGCTTCCACCAGCCCATCTACTACCTGCTCGGCAATCTG; GCCGCGGCTGACCTCTTCGCGGGCGTGGCCTACCTCTTCCTCATGTTCCACACTGGTCCC; CGCACAGCCCGACTTTCACTTGAGGGCTGGTTCCTGCGGCAGGGCTTGCTGGACACAAGC; CTCACTGCGTCGGTGGCCACACTGCTGGCCATCGCCGTGGAGCGGCACCGCAGTGTGATG; GCCGTGCAGCTGCACAGCCGCCTGCCCCGTGGCCGCGTGGTCATGCTCATTGTGGGCGTG; TGGGTGGCTGCCCTGGGCCTGGGGCTGCTGCCTGCCCACTCCTGGCACTGCCTCTGTGCC; CTGGACCGCTGCTCACGCATGGCACCCCTGCTCAGCCGCTCCTATTTGGCCGTCTGGGCT; CTGTCGAGCCTGCTTGTCTTCCTGCTCATGGTGGCTGTGTACACCCGCATTTTCTTCTAC; GTGCGGCGGCGAGTGCAGCGCATGGCAGAGCATGTCAGCTGCCACCCCCGCTACCGAGAG; ACCACGCTCAGCCTGGTCAAGACTGTTGTCATCATCCTGGGGGCGTTCGTGGTCTGCTGG; ACACCAGGCCAGGTGGTACTGCTCCTGGATGGTTTAGGCTGTGAGTCCTGCAATGTCCTG; GCTGTAGAAAAGTACTTCCTACTGTTGGCCGAGGCCAACTCACTGGTCAATGCTGCTGTG; TACTCTTGCCGAGATGCTGAGATGCGCCGCACCTTCCGCCGCCTTCTCTGCTGCGCGTGC; CTCCGCCAGTCCACCCGCGAGTCTGTCCACTATACATCCTCTGCCCAGGGAGGTGCCAGC; ACTCGCATCATGCTTCCCGAGAACGGCCACCCACTGATGGACTCCACCCTTTAGCTACCT; TGAACTTCAGCGGTACGCGGCAAGCAACAAATCCACAGCCCCTGATGACTTGTGGGTGCT; CCTGGCTCAACCCAACCAACAGGACTGACTGACTGGCAGGACAAGGTCTGGCATGGCACA; GCACCACTGCCAGGCCTCCCCAGGCACACCACTCTGCCCAGGGAATGGGGGCTTTGGGTC; ATCTCCCACTGCCTGGGGGAGTCAGATGGGGTGCAGGAATCTGGCTCTTCAGCCATCTCA; GGTTTAGGGGGTTTGTAACAGACATTATTCTGTTTTCACTGCGTATCCTTGGTAAGCCCT; GTGGACTGGTTCCTGCTGTGTGATGCTGAGGGTTTTAAGGTGGGGAGAGATAAGGGCTCT; CTCGGGCCATGCTACCCGGTATGACTGGGTAATGAGGACAGACTGTGGACACCCCATCTA; CCTGAGTCTGATTCTTTAGCAGCAGAGACTGAGGGGTGCAGAGTGTGAGCTGGGAAAGGT; TTGTGGCTCCTTGCAGCCTCCAGGGACTGGCCTGTCCCCGATAGAATTGAAGCAGTCCAC; GGGGAGGGGATGATACAAGGAGTAAACCTTTCTTTACACTCAAAAAAAAAAAAAAAAAAA; AAAAAAAAAAACTCGAC;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004720 unedited; NGGTCAAATTTGTATACGACTCCTATAGGGCGGCCGCGATTCGGCACGAGGGCAACCCGC; ACAGGGCGCACCCGGACGCTCTACCGCTCCCGCCGCAGTCGCCGGGCCATGGGCCTCGAG; CCCGCCCCGAACCCCCGCGAGCCCGCCTTGTCTGCGGCGTGACTGGAGGCCCAGATGGTC; ATCATGGGCCAGTGCTACTACAACGAGACCATCGGCTTCTTCTATAACAACAGTGGCAAA; GAGCTCAGCTCCCACTGGCGGCCCAAGGATGTGGTCGTGGTGGCACTGGGGCTGACCGTC; AGCGTGCTGGTGCTGCTGACCAATCTGCTGGTCATAGCAGCCATCGCCTCCAACCGCCGC; TTCCACCAGCCCATCTACTACCTGCTCGGCAATCTGGCCGCGGCTGACCTCTTCGCGGGC; GTGGCCTACCTCTTCCTCATGTTCCACACTGGTCCCCGCACAGCCCGACTTTCACTTGAG; GGCTGGTTCCTGCGGCAGGGCTTGCTGGACACAAGCCTCACTGCGTCGGTGGCCACACTG; CTGGCCATCGCCGTGGAGCGGCACCGCAGTGTGATGGCCGTGCAGCTGCACAGCCGCCTG; CCCCGTGGCCGCGTGGTCATGCTCATTGTGGGCGTGTGGGTGGCTGCCCTGNGCCTGGGG; CTGCTGCCTGCCCACTCCTGGCACTGCCTCTGTGCCCTGGACCGCTGCTCACGCATGGCA; CCCCTGCTCAGCCGCTCCTATTTGGCCGTCTGGGCTCTGTCGAGCCTGCTTGTCTTCCTG; CTCATGGGGNCTGTGTACACCCGCATTNTCTTTCTACGTGCGCGGCGAGTGCAGCGCATG; GCANAGCATGTCAGCTGCCACCCCCGCTACCGAGAGACCACGCTCAGCCTGGTCAGAACT; GNTGTCATCATCTGG;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004720 unedited; GGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGAGGGGAAAG; AAAGGGTTACTCCTTGGATCATCCCCTCCCCGTGGACTGCTTCAATTCTATCGGGGACAG; GCCAGTCCCTGGAGGCTGCAAGGAGCCACAAACCTTTCCCAGCTCACACTCTGCACCCCT; CAGTCTCTGCTGCTAAAGAATCAGACTCAGGTAGATGGGGTGTCCACAGTCTGTCCTCAT; TACCCAGTCATACCGGGTAGCATGGCCCGAGAGAGCCCTTATCTCTCCCCACCTTAAAAC; CCTCAGCATCACACAGCAGGAACCAGTCCACAGGGCTTACCAAGGATACGCAGTGAAAAC; AGAATAATGTCTGTTACAAACCCCCTAAACCTGAGATGGCTGAAGAGCCAGATTCCTGCA; CCCCATCTGACTCCCCCAGGCAGTGGGAGATGACCCAAAGCCCCCATTCCCTGGGCAGAG; TGGTGTGCCTGGGGAGGCCTGGCAGTGGTGCTGTGCCATGCCAGACCTTGTCCTGCCAGT; CAGTCAGTCCTGTTGGTTGGGTTGAGCCAGGAGCACCCACAAGTCATCAGGGGCTGTGGA; TTTGTTGCTTGCCGCGTACCGCTGAAGTTCAAGGTAGCTAAAGGGTGGAGTCCATCAGTG; GGTGGCCGTTCTCGGGAAGCATGATGCGAGTGCTGGCACCTCCCTGGGCAGAGGATGTAT; AGTGGACAGACTCGCGGGTGGACTGGNCGGAGCCGCGCANCANAGAAGCCGCGAAGGTGC; GGCGCATCTAGCATCTCGCAAGAGTCACAGCAGCTTGACAGTGNAGTGGCCTCCGCCAAC; AGTAGAGTACTTTTCTCAGCCAGACATGCAGACTCCAGCCCTAACTCCAGGACAGTACAC; CTGCCTGGGTCCACAGACACGACGCCCCAGAGAGACACATTTGACAGCTGA;
• Restriction Sites: NotI-NotI
• ACCN: NM_004720
• Insert Size: 1790 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_004720.4, NP_004711.2
• RefSeq Size: 1868 bp
• RefSeq ORF: 1056 bp
• Locus ID: 9170
• UniProt ID: Q9HBW0
• Cytogenetics: 19p13.11
• Domains: 7tm_1
• Protein Families: Druggable Genome, GPCR, Transmembrane
• Protein Pathways: Neuroactive ligand-receptor interaction
• Summary: This gene encodes a member of family I of the G protein-coupled receptors, as well as the EDG family of proteins. This protein functions as a lysophosphatidic acid (LPA) receptor and contributes to Ca2+ mobilization, a critical cellular response to LPA in cells, through association with Gi and Gq proteins. An alternative splice variant has been described but its full length sequence has not been determined. [provided by RefSeq, Jul 2008]