MTA1 (NM_004689) Human Untagged Clone

CAT#: SC117199

MTA1 (untagged)-Human metastasis associated 1 (MTA1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MTA1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC117199 sequence for NM_004689 edited (data generated by NextGen Sequencing)
  ATGGCCGCCAACATGTACAGGGTCGGAGACTACGTCTACTTTGAGAACTCCTCCAGCAAC
  CCATACCTGATCCGGAGAATCGAGGAGCTCAACAAGACGGCCAATGGGAACGTGGAGGCC
  AAAGTGGTGTGCTTCTACCGGAGGCGGGACATCTCCAGCACCCTCATCGCCCTGGCCGAC
  AAGCACGCAACCCTGTCAGTCTGCTATAAGGCCGGACCGGGGGCGGACAACGGCGAGGAA
  GGGGAAATAGAAGAGGAAATGGAGAATCCGGAAATGGTGGACCTGCCCGAGAAACTAAAG
  CACCAGCTGCGGCATCGGGAGCTGTTCCTCTCCCGGCAGCTGGAGTCTCTGCCCGCCACG
  CACATCAGGGGCAAGTGCAGCGTCACCCTGCTCAACGAGACCGAGTCGCTCAAGTCCTAC
  CTGGAGCGGGAGGATTTCTTCTTCTATTCTCTAGTCTACGACCCACAGCAGAAGACCCTG
  CTGGCAGATAAAGGAGAGATTCGAGTAGGAAACCGGTACCAGGCAGACATCACCGACTTG
  TTAAAAGAAGGCGAGGAGGATGGCCGAGACCAGTCCAGGTTGGAGACCCAGGTGTGGGAG
  GCGCACAACCCACTCACAGACAAGCAGATCGACCAGTTCCTGGTGGTGGCCCGCTCTGTG
  GGCACCTTCGCACGGGCCCTGGACTGCAGCAGCTCCGTCCGACAGCCCAGCCTGCACATG
  AGCGCCGCAGCTGCCTCCCGAGACATCACCCTGTTCCACGCCATGGATACTCTCCACAAG
  AACATCTACGACATCTCCAAGGCCATCTCGGCGCTGGTGCCGCAGGGCGGGCCCGTGCTC
  TGCAGGGACGAGATGGAGGAGTGGTCTGCATCAGAGGCCAACCTTTTCGAGGAAGCCCTG
  GAAAAATATGGGAAGGATTTCACGGACATTCAGCAAGATTTTCTCCCGTGGAAGTCGCTG
  ACCAGCATCATTGAGTACTACTACATGTGGAAGACCACCGACAGATACGTGCAGCAGAAA
  CGCTTGAAAGCAGCTGAAGCTGAGAGCAAGTTAAAGCAAGTTTATATTCCCAACTATAAC
  AAGCCAAATCCGAACCAAATCAGCGTCAACAACATCAAGGCCGGTGTGGTGAACGGCACG
  GGGGCGCCGGGCCAGAGCCCTGGGGCTGGCCGGGCCTGCGAGAGCTGTTACACCACACAG
  TCTTACCAGTGGTATTCTTGGGGTCCCCCTAACATGCAGTGTCGTCTCTGCGCATCTTGT
  TGGACATATTGGAAGAAATATGGTGGCTTGAAAATGCCAACCCGGTTAGATGGAGAGAGG
  CCAGGACCAAACCGCAGTAACATGAGTCCCCACGGCCTCCCAGCCCGGAGCAGCGGGAGC
  CCCAAGTTTGCCATGAAGACCAGGCAGGCTTTCTATCTGCACACGACGAAGCTGACGCGG
  ATCGCCCGGCGCCTGTGCCGTGAGATCCTGCGCCCGTGGCACGCTGCGCGGCACCCCTAC
  CTGCCCATCAACAGCGCGGCCATCAAGGCCGAGTGCACGGCGCGGCTGCCCGAAGCCTCC
  CAGAGCCCGCTGGTGCTGAAGCAGGCGGTACGCAAGCCGCTGGAAGCCGTGCTTCGGTAT
  CTTGAGACCCACCCCCGCCCCCCCAAGCCTGACCCCGTGAAAAGCGTGTCCAGCGTGCTC
  AGCAGCCTGACGCCCGCCAAGGTGGCCCCCGTCATCAACAACGGCTCCCCCACCATCCTG
  GGCAAGCGCAGCTACGAGCAGCACAACGGGGTGGACGGCAACATGAAGAAGCGCCTCTTG
  ATGCCCAGTAGGGGTCTGGCAAACCACGGACAGACCAGGCACATGGGACCAAGCCGGAAC
  CTCCTGCTCAACGGGAAGTCCTACCCCACCAAAGTGCGCCTGATCCGGGGGGGCTCCCTG
  CCCCCAGTCAAGCGGCGGCGGATGAACTGGATCGACGCCCCGGATGACGTGTTCTACATG
  GCCACAGAGGAGACCAGGAAGATCCGCAAGCTGCTCTCATCCTCGGAAACCAAGCGTGCT
  GCCCGCCGGCCCTACAAGCCCATCGCCCTGCGCCAGAGCCAGGCCCTGCCGCCGCGGCCA
  CCGCCACCTGCGCCCGTCAACGACGAGCCCATCGTCATCGAGGACTAG
  
  Clone variation with respect to NM_004689.3
  1114 g=>a;1834 g=>a
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004689 unedited
  AGAGTCGCAATTTGTATACCACTCACTATAGGCGGCCGCGNAATTCGCACGAGGCGGCCC
  TCCCGTCCCTGCGCGGCCTCGGCGGCCTCGGCGGCGGCGGCGGCGGCGGCGGCGGCAGCA
  GCGCGGCCCCTTTAAACGCCTGCGGCGCCCCCCGCCCCCGCCATCGCGCCTCCATTTTCC
  CGGCCGCCCGCGCCGAGCGCCGCGCCCGCCCCGGGCCCCTCCGCCGCCGCCGGCCCGGAC
  ATGGCCGCCAACATGTACAGGGTCGGAGACTACGTCTACTTTGAGAACTCCTCCAGCAAC
  CCATACCTGATCCGGAGAATCGAGGAGCTCAACAAGACGGCCAATGGGAACGTGGAGGCC
  AAAGTGGTGTGCTTCTACCGGAGGCGGGACATCTCCAGCACCCTCATCGCCCTGGCCGAC
  AAGCACGCAACCCTGTCAGTCTGCTATAAGGCCGGACCGGGGGCGGACAACGGCGAGGAA
  GGGGAAATAGAAGAGGAAATGGAGAATCCGGAAATGGTGGACCTGCCCGAGAAACTAAAG
  CACCAGCTGCGGCATCGGGAGCTGTTCCTCTCCCGGCAGCTGGAGTCTCTGCCCGCCACG
  CACATCAGGGGCAAGTGCAGCGTCACCCTGCTCAACGAGACCGAGTCGCTCAAGTCCTAC
  CTGGAGCGGGAGGATTTCTTCTTCTATTCTCTAGTCTACGACCCACAGCAGAAGACCCTG
  CTGGCAGATAAAGGAGAGATTCGAGTAGGAAACCGGTACCAGGCAGACATCACCGACTTG
  TTAAAAGAAGGCGAGGAGGATGGCCGAGACCAGTCCAGGTTGGAGACCCAGGTGTGGGAG
  GCGCACAACCCACTCACANGACAGCAGATCGACCAGTTCCTGGGTGGTGGCCCGCTCTGT
  GGGCACCTTCGCACGGGCCCTGGACTGCAGCAGCTCCGTCCGACAGCCCAGCCTGCN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004689 unedited
  ACCGCGGCCGCATCTACATCGGTTTTTTTTTTTTTTTTTTGTTAATATGAAAATTACTTG
  AAAAGACAAGGGCCAACCCCGCCAGGCAGCTCGGCCGGCCGCGGCCGCACGATCCTAACA
  TTCCAGGTGTAAGTTACAGAGCTCAAGTTCATCTACAAAAAAAGTAATAAAAATAAAATT
  TAAAATGGCACCTTCAACAGAACACAACAAAACCTTAGGGCCCGGCCTCCGGGCAAAAGG
  CCCCGCAGCACAGGGGCACGGGGTGGCCCCTCATCTCCGCTCCAGCCAAAAAGTAAACAC
  AAAGCTAAAAACGACAACTCCTCGGCATTCTCGGAATAAGTTAGCCGCGCTTCTTCCTCT
  CCTCCCCCAGTGTCCGCCAAGGAGCGCGTTTCTCTGCAGGTGAGGGCGGCACGTGGGCGC
  ACTACCAAACTGAGGGGCGGGCGGCGGGCTGGCTGGGAAGGGGCCCCGCGGGCGGGCCAG
  GGGCGGGGGGCGGACGCAAGTGGGGGCGGACCCTAGTCCTCCATGACGATGCGCTCGTCC
  CTGACGGCCGCATGTGGCGGCGGCCGCGGCGGCACGGTCTTGCTCTGGCCACCGCCATGG
  GCTTGTCCGGCCGCCGGCAACTACGCTTTGCTCCCGAGACGACAGCACCTTGCGGATCTT
  TCTTGGCCTCCCCCTGGGCCCTGACAACCAGCCACCCGGCCCGTTGATCATTTCACCGCC
  TCCGTTTGACTGGCGCATGGACCCTCCTCGATCAAGCCAATTCGGCGGGTACGACTTCCC
  GCTTACACGCAGTCACGGCTTGCCCCATGGCCCGCCCGCCCGCGTTTTCCCCCCCCCTAT
  GGGTTTTACCAGCCCCTCTTTATGGTTCCCCCTCACCCGCCTTGCTCGCCCCACTCCCCT
  TGCCAAAAGCGTGGCAAACCGTCCT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_004689
• Insert Size: 2320 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004689.2, NP_004680.1
• RefSeq Size: 2662 bp
• RefSeq ORF: 2148 bp
• Locus ID: 9112
• UniProt ID: Q13330
• Cytogenetics: 14q32.33
• Domains: GATA, ELM2, myb_DNA-binding, BAH
• Protein Families: Druggable Genome, Transcription Factors
• Gene Summary: This gene encodes a protein that was identified in a screen for genes expressed in metastatic cells, specifically, mammary adenocarcinoma cell lines. Expression of this gene has been correlated with the metastatic potential of at least two types of carcinomas although it is also expressed in many normal tissues. The role it plays in metastasis is unclear. It was initially thought to be the 70kD component of a nucleosome remodeling deacetylase complex, NuRD, but it is more likely that this component is a different but very similar protein. These two proteins are so closely related, though, that they share the same types of domains. These domains include two DNA binding domains, a dimerization domain, and a domain commonly found in proteins that methylate DNA. The profile and activity of this gene product suggest that it is involved in regulating transcription and that this may be accomplished by chromatin remodeling. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2011]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (MTA1).