PAPSS2 (NM_004670) Human Untagged Clone

CAT#: SC117190

PAPSS2 (untagged)-Human 3'-phosphoadenosine 5'-phosphosulfate synthase 2 (PAPSS2), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PAPSS2
• Synonyms: ATPSK2; BCYM4; SK2
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_004670, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGTCGGGGATCAAGAAGCAAAAGACGGAGAACCAGCAGAAATCCACCAATGTAGTCTATCAGGCCCACC
  ATGTGAGCAGGAATAAGAGAGGGCAAGTGGTTGGAACAAGGGGTGGGTTCCGAGGATGTACCGTGTGGCT
  AACAGGTCTCTCTGGTGCTGGAAAAACAACGATAAGTTTTGCCCTGGAGGAGTACCTTGTCTCCCATGCC
  ATCCCTTGTTACTCCCTGGATGGGGACAATGTCCGTCATGGCCTTAACAGAAATCTCGGATTCTCTCCTG
  GGGACAGAGAGGAAAATATCCGCCGGATTGCTGAGGTGGCTAAGCTGTTTGCTGATGCTGGTCTGGTCTG
  CATTACCAGCTTTATTTCTCCATTCGCAAAGGATCGTGAGAATGCCCGCAAAATACATGAATCAGCAGGG
  CTGCCATTCTTTGAAATATTTGTAGATGCACCTCTAAATATTTGTGAAAGCAGAGACGTAAAAGGCCTCT
  ATAAAAGGGCCAGAGCTGGGGAGATTAAAGGATTTACAGGTATTGATTCTGATTATGAGAAACCTGAAAC
  TCCTGAGCGTGTGCTTAAAACCAATTTGTCCACAGTGAGTGACTGTGTCCACCAGGTAGTGGAACTTCTG
  CAAGAGCAGAACATTGTACCCTATACTATAATCAAAGATATCCACGAACTCTTTGTGCCGGAAAACAAAC
  TTGACCACGTCCGAGCTGAGGCTGAAACTCTCCCTTCATTATCAATTACTAAGCTGGATCTCCAGTGGGT
  CCAGGTTTTGAGCGAAGGCTGGGCCACTCCCCTCAAAGGTTTCATGCGGGAGAAGGAGTACTTACAGGTT
  ATGCACTTTGACACCCTGCTAGATGATGGCGTGATCAACATGAGCATCCCCATTGTACTGCCCGTCTCTG
  CAGAGGATAAGACACGGCTGGAAGGGTGCAGCAAGTTTGTCCTGGCACATGGTGGACGGAGGGTAGCTAT
  CTTACGAGACGCTGAATTCTATGAACACAGAAAAGAGGAACGCTGTTCCCGTGTTTGGGGGACAACATGT
  ACAAAACACCCCCATATCAAAATGGTGATGGAAAGTGGGGACTGGCTGGTTGGTGGAGACCTTCAGGTGC
  TGGAGAAAATAAGATGGAATGATGGGCTGGACCAATACCGTCTGACACCTCTGGAGCTCAAACAGAAATG
  TAAAGAAATGAATGCTGATGCGGTGTTTGCATTCCAGTTGCGCAATCCTGTCCACAATGGCCATGCCCTG
  TTGATGCAGGACACTCGCCGCAGGCTCCTAGAGAGGGGCTACAAGCACCCGGTCCTCCTACTACACCCTC
  TGGGCGGCTGGACCAAGGATGACGATGTGCCTCTAGACTGGCGGATGAAGCAGCACGCGGCTGTGCTCGA
  GGAAGGGGTCCTGGATCCCAAGTCAACCATTGTTGCCATCTTTCCGTCTCCCATGTTATATGCTGGCCCC
  ACAGAGGTCCAGTGGCACTGCAGGTCCCGGATGATTGCGGGTGCCAATTTCTACATTGTGGGGAGGGACC
  CTGCAGGAATGCCCCATCCTGAAACCAAGAAGGATCTGTATGAACCCACTCATGGGGGCAAGGTCTTGAG
  CATGGCCCCTGGCCTCACCTCTGTGGAAATCATTCCATTCCGAGTGGCTGCCTACAACAAAGCCAAAAAA
  GCCATGGACTTCTATGATCCAGCAAGGCACAATGAGTTTGACTTCATCTCAGGAACTCGAATGAGGAAGC
  TCGCCCGGGAAGGAGAGAATCCCCCAGATGGCTTCATGGCCCCCAAAGCATGGAAGGTCCTGACAGATTA
  TTACAGGTCCCTGGAGAAGAACTAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004670 unedited
  NAAATAATAACCCCCGCCCGTTGNCGCAAAGGGCGGTAGGCGTGTACGGTGGGAGGTCTA
  TATAAGCAGAGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGC
  GGCCGCGAATTCGGCACGAGGCCGCCGCCGCCGCCGCCGTCCCTGCGTCCTTCGGTCTCT
  GCTCCCGGGACCCGGGCTCCGCCGCAGCCAGCCAGCATGTCGGGGATCAAGAAGCAAAAG
  ACGGAGAACCAGCAGAAATCCACCAATGTAGTCTATCAGGCCCACCATGTGAGCAGGAAT
  AAGAGAGGGCAAGTGGTTGGAACAAGGGGTGGGTTCCGAGGATGTACCGTGTGGCTAACA
  GGTCTCTCTGGTGCTGGAAAAACAACGATAAGTTTTGCCCTGGAGGAGTACCTTGTCTCC
  CATGCCATCCCTTGTTACTCCCTGGATGGGGACAATGTCCGTCATGGCCTTAACAGAAAT
  CTCGGATTCTCTCCTGGGGACAGAGAGGAAAATATCCGCCGGATTGCTGAGGTGGCTAAG
  CTGTTTGCTGATGCTGGTCTGGTCTGCATTACCAGCTTTATTTCTCCATTCGCAAAGGAT
  CGTGAGAATGCCCGCAAAATACATGAATCAGCAGGGCTGCCATTCTTTGAAATATTTGTA
  GATGCACCTCTAAATATTTGTGAAAGCAGAGACGTAAAAGGCCTCTATAAAAGGGCCAGA
  GCTGGGGAGATTAAAGGATTTACAGGTATTGATTCTGATTATGAGAAACCTGAAACTCCT
  GAGCGTGTGCTTAAACCAATTTGTCCCAGTGAGTGACTGTGNTCACCAGGTAGTGTAACT
  TCTGCAGAGCAGACATTGTACCTATACTATAATCAAGAATCCACGACTCTTGTGCCGTAC
  ACAACTTG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004670 unedited
  NGGTATTTACTATGTNACCGCGGCCGCTTTTCTANNATCGAGTTTTTTTTTTTTTTTTTT
  AAAGGCGTAGTGAGTAACCGATTACATGAGTATAGGCACTATACTATTAAAGACAAACTG
  CTGTGGCCATTGTGTGTAACTTATTCTCAAAAGAACTTTTGTTAGTTAAGATTAGTGTTT
  TTTAAGAATGAGAATATGAAAATTGTCACAGTTTTCTCTTTCTAATTTAGGTCTCTTCCA
  GAGAGACCTATATATTAAAAGGTGCTGTTTCTTCACTTTATTCCTTGGCAACAATGAATT
  ATGCATAAAGTAAAAACATTGCCTCTCAGGAAGTAAAGCCATCAGAAGATGTCTCAGAAC
  AGCTTGAAATCTTTGGAATAGCTTTTTTTTTTTTTCAACTGCAATAAAATCAGTGCAGTT
  CAGAAAACTCGACCTTTCAGTATCCGAGAAGGCAGCTTTGTAAGCACTTTTTGTTCGAGG
  AACTTTGTTTAACCGCTGAGGGGAATCTGACCCAGCTCCTGGGTTATCTGGTGTAAACAG
  GGCACCCCACTGGGAGTTAAGTGGGCTGGGGGCTTTTTTACTGGCCCCAGCCCTTCCTAA
  TAATGGGCAAATTACCTTTTTGTACGGGGCTCACCGCTTAAAAAACACATACTGTGGCTC
  ATTACAGTTTGTTCACCTGTTAAAGAAAGGGTGGACTCTTTGGTTTCTCGGGGGCCTTTC
  CCCCTTTTAGGGCCCTTGCTGTCTCGAAAAGGGGAATATTTTTTTATACACACTGGCCTC
  TTTCCTCGTTCAGGGCCTTCCTTTTTTGCCGAAACCCTTGTCCCCCGCAAACGGGGGAAG
  AAAATTTTTCCCACCATTGCCCCCTTGGTGGAAGTCCTCTTTTCCAAAACCGGTTGCCTT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_004670
• Insert Size: 4700 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004670.3, NP_004661.2
• RefSeq Size: 3859 bp
• RefSeq ORF: 1845 bp
• Locus ID: 9060
• UniProt ID: O95340
• Cytogenetics: 10q23.2-q23.31
• Domains: ATP-sulfurylase, APS_kinase
• Protein Families: Druggable Genome
• Protein Pathways: Metabolic pathways, Purine metabolism, Selenoamino acid metabolism, Sulfur metabolism
• Gene Summary: Sulfation is a common modification of endogenous (lipids, proteins, and carbohydrates) and exogenous (xenobiotics and drugs) compounds. In mammals, the sulfate source is 3'-phosphoadenosine 5'-phosphosulfate (PAPS), created from ATP and inorganic sulfate. Two different tissue isoforms encoded by different genes synthesize PAPS. This gene encodes one of the two PAPS synthetases. Defects in this gene cause the Pakistani type of spondyloepimetaphyseal dysplasia. Two alternatively spliced transcript variants that encode different isoforms have been described for this gene. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (1) represents the more frequently occurring transcript. It encodes isoform a, which has also been named PAPS synthase 2a.