Beta 1,4 galactosyltransferase 6 (B4GALT6) (NM_004775) Human Untagged Clone

SKU SC117169

B4GALT6 (untagged)-Human UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 6 (B4GALT6)

Product Data

• Type: Human Untagged Clone
• Target Symbol: Beta 1,4 galactosyltransferase 6
• Synonyms: B4Gal-T6; beta4Gal-T6
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC117169 sequence for NM_004775 edited (data generated by NextGen Sequencing); ATGTCTGTGCTCAGGCGGATGATGCGGGTTTCCAATCGCTCTCTCCTCGCCTTCATCTTC; TTCTTCTCCCTCTCTTCGTCCTGTCTGTACTTCATCTATGTGGCCCCAGGCATCGCCAAC; ACATATCTCTTTATGGTACAAGCTCGAGGTATAATGTTGAGAGAAAATGTGAAAACAATA; GGTCATATGATCAGGCTGTACACAAATAAAAACAGTACGCTCAACGGTACAGATTATCCC; GAAGGCAATAATTCAAGTGATTATCTTGTTCAAACAACAACGTATCTCCCGGAAAACTTC; ACATACTCACCATACCTCCCCTGTCCAGAAAAGCTGCCTTATATGCGAGGATTCCTCAAT; GTCAATGTAAGCGAAGTCAGTTTTGATGAAATTCATCAACTCTTCTCCAAGGATTTAGAT; ATTGAGCCAGGGGGTCATTGGAGGCCAAAAGACTGTAAACCCAGATGGAAGGTGGCAGTT; CTCATTCCTTTCCGTAATCGCCATGAACATCTTCCAATTTTTTTCTTACATCTGATTCCA; ATGCTCCAGAAGCAGCGGCTGGAATTTGCGTTTTATGTCATTGAACAGACTGGCACACAA; CCTTTTAACCGTGCGATGCTTTTCAATGTGGGCTTCAAAGAGGCCATGAAAGACAGTGTC; TGGGACTGTGTAATCTTCCACGATGTGGATCATCTACCTGAAAATGACCGGAACTATTAC; GGATGTGGAGAAATGCCACGTCATTTTGCTGCAAAGCTGGATAAATACATGTATATTCTT; CCATATAAAGAATTTTTTGGTGGTGTAAGTGGGCTGACAGTGGAACAATTTAGAAAGATC; AATGGTTTTCCTAATGCCTTCTGGGGATGGGGAGGAGAAGATGATGACCTTTGGAACAGA; GTTCACTATGCTGGATATAATGTAACCAGACCAGAGGGAGACTTAGGAAAATACAAGTCA; ATTCCTCATCACCATAGAGGTGAAGTCCAGTTTTTAGGACGGTATAAATTACTAAGGTAT; TCCAAGGAGCGTCAGTACATCGATGGACTGAACAATTTAATATATAGGCCAAAAATACTG; GTTGATAGGTTGTATACAAACATATCTGTAAACCTCATGCCAGAGTTAGCTCCAATCGAA; GACTATTAA; ; Clone variation with respect to NM_004775.3;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004775 unedited; GTTCGAATTTTGTAATACGACTCACTATAGGGGCGGCCGCGAATTCGCACGAGCCCTCTG; GCCTCCCATTCATTCCGTCCGCTCCCTCCCATTTCCTCTTTTCCGCTCGGGCCCTCTCCC; CAGAGCCTCCCCGCGCGGACCCCGCTGCAGTCTCGGGGTTCTCAGCACATTTATGGAAGT; TTAGGGCCTGGACAGTGGCCCCGAGTCCGGCCTGAGAGCGCAGCCTGGGCTGCTGGCAGG; GAAGAGGAAGATGTCTGTGCTCAGGCGGATGATGCGGGTTTCCAATCGCTCTCTCCTCGC; CTTCATCTTCTTCTTCTCCCTCTCTTCGTCCTGTCTGTACTTCATCTATGTGGCCCCAGG; CATCGCCAACACATATCTCTTTATGGTACAAGCTCGAGGTATAATGTTGAGAGAAAATGT; GAAAACAATAGGTCATATGATCAGGCTGTACACAAATAAAAACAGTACGCTCAACGGTAC; AGATTATCCCGAAGGCAATAATTCAAGTGATTATCTTGTTCAAACAACAACGTATCTCCC; GGAAAACTTCACATACTCACCATACCTCCCCTGTCCAGAAAAGCTGCCTTATATGCGAGG; ATTCCTCAATGTCAATGTAAGCGAAGTCAGTTTTGATGAAATTCATCAACTCTTCTCCAA; GGATTTAGATATTGAGCCAGGGGGTCATTGGAGGCCAAAAGACTGTAAACCCAGATGGGA; AGGTGGCAGTTCTCATTCCTTTCCGTAATCGCCATGAACATCTTCCAATTTTTTTCTTAC; ATCTGATTCCAATGCTCCAGAAGCAGCGGCTGGGAAATTTGCGTTTATGTCATTGAACAG; ACTGGCACACAACCTTTTAACCGTGCGATGCTTTTCAATGTGGGCTTNCANNAGAGCCAT; GAAGACAGTGTCTGGGACTGTGGTATCTTCCCACGATGTGGATCAT;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004775 unedited; ACTATGGCCCGGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTGCATTTGCA; ATGTATTTGTTAATCTTTATGTAATAATTAATACAATGAAGATAAACGGGCACAGCTCTG; TCACTCTCACAGAAACATATTAAGTTCACTGTTTATAGGCAAAAATAATTTTAGGTCTAC; ACTAAAAAAGCAAAACAAACAAACAAAAAGCAAACGAGGTGGTTTTCACAGGATGACAAA; CTATATTTCAAAACTGAAAAAAAGCAAAATGTTTATATCTCACTCCTGAAACAAAAATTA; ACATCAGACTTAAGAAAATAAGGCAGATACTAGTAGTACTAAGTTTTCTTGAAACTGTAA; AATATATATAAAAATGAAAAGATACCGAATGTGGACAGCTCCACATTGATCAACAAATGT; TAACATTCTCAATCTCTTTCATTGACTTGAAAAACTATGTAATAGAAACAGAAAATGAAC; TAATACACAAATGAAGTACAAATATCATAATTTTCAGAAGGTTTGATTTTTCGAGTACCA; TAAAAAAACTGAAATATAAATATTTTGGAAATAGTTCTAAGAAATAAATATGAAAATATT; TTGGTTGGTATCATAACACAGAAGCTATCCATTTTCTCAAAGTGTAAGGATTCAATTATT; TTATGATTTAGCTTGCGGTCATTTGTGTTATCAATATTGCCATAAAAATGACACTTCAAA; ATAAAACTTACAATCTGAGATAAATAACCATAGCTCTTGTTTAGGTAAACAATCCTTGAG; GCGAATATTAATCACACGGTCCTCATATATAAAACCTCTATTGCTCACCACTGGTGGTGA; ACATTCACCTTTTACAACATCAATTTTTCGTTTAAAATTCGGAAAATTACGACATTTAAA; CATCTTTACAATCCCAATTCACAAAACAATATGGTTCGCCTTACTGGGAAAAATATTTTC; AAACCAA;
• Restriction Sites: NotI-NotI
• ACCN: NM_004775
• Insert Size: 4000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_004775.2, NP_004766.1
• RefSeq Size: 3830 bp
• RefSeq ORF: 1149 bp
• Locus ID: 9331
• UniProt ID: Q9UBX8
• Cytogenetics: 18q12.1
• Domains: Galactosyl_T_2
• Protein Families: Transmembrane
• Protein Pathways: Metabolic pathways, Sphingolipid metabolism
• Summary: This gene is one of seven beta-1,4-galactosyltransferase (beta4GalT) genes in human. They encode type II membrane-bound glycoproteins that appear to have exclusive specificity for the donor substrate UDP-galactose; all transfer galactose in a beta1,4 linkage to similar acceptor sugars: GlcNAc, Glc, and Xyl. Each beta4GalT has a distinct function in the biosynthesis of different glycoconjugates and saccharide structures. As type II membrane proteins, they have an N-terminal hydrophobic signal sequence that directs the protein to the Golgi apparatus and which then remains uncleaved to function as a transmembrane anchor. This gene produces multiple protein isoforms - some of which are predicted to lack the N-terminal hydrophobic signal sequence and transmembrane domain. By sequence similarity, the beta4GalTs form four groups: beta4GalT1 and beta4GalT2, beta4GalT3 and beta4GalT4, beta4GalT5 and beta4GalT6, and beta4GalT7. The canonical enzyme encoded by this gene is a lactosylceramide synthase important for glycolipid biosynthesis. [provided by RefSeq, Jan 2020]