COPB2 (NM_004766) Human Untagged Clone

CAT#: SC117165

COPB2 (untagged)-Human coatomer protein complex, subunit beta 2 (beta prime) (COPB2), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: COPB2
• Synonyms: beta'-COP; MCPH19
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_004766 edited
  GAATTCGGCACGAGGGTCAGTGGATCGGTGGGTTTATCTCAAGGCCTGAGTAGCCGGTAA
  CAAACGAGGGTTCCCGGGATTGGACCGACGCAGCCATGCCTCTGCGACTTGATATCAAAA
  GAAAGCTAACTGCTAGATCTGATCGAGTTAAGAGTGTGGATCTGCATCCTACAGAGCCAT
  GGATGTTGGCAAGTCTTTACAATGGCAGTGTGTGTGTTTGGAATCATGAAACACAGACAC
  TGGTGAAGACATTTGAAGTATGTGATCTTCCTGTTCGAGCTGCAAAGTTTGTTGCAAGGA
  AGAATTGGGTTGTGACAGGAGCGGATGACATGCAGATTAGAGTGTTCAATTACAATACTC
  TGGAGAGAGTTCATATGTTTGAAGCACACTCAGACTACATTCGCTGTATTGCTGTTCATC
  CAACCCAGCCTTTCATTCTAACTAGCAGTGATGACATGCTTATTAAGCTCTGGGACTGGG
  ATAAAAAATGGTCTTGCTCACAAGTGTTTGAAGGACACACCCATTATGTTATGCAGATTG
  TGATCAACCCCAAAGATAACAATCAGTTTGCCAGTGCCTCTTTGGACAGGACTATCAAGG
  TGTGGCAGTTGGGCTCTTCGTCACCAAACTTCACTTTGGAAGGACATGAGAAAGGCGTGA
  ATTGCATTGATTACTACAGTGGTGGGGACAAGCCATACCTCATTTCAGGTGCAGATGACC
  GTCTTGTTAAAATATGGGATTATCAGAATAAAACATGTGTGCAGACACTGGAAGGACATG
  CCCAAAATGTGTCTTGTGCCAGCTTTCATCCTGAGTTGCCAATCATTATCACAGGTTCAG
  AAGATGGAACAGTACGTATTTGGCATTCAAGCACCTACCGGCTTGAGAGCACACTGAATT
  ATGGAATGGAGAGGGTATGGTGCGTGGCCAGTCTAAGAGGGTCAAACAATGTCGCTTTGG
  GCTATGATGAAGGGAGCATCATTGTTAAGCTTGGTCGGGAGGAACCTGCCATGTCCATGG
  ATGCCAATGGAAAGATAATTTGGGCCAAGCATTCAGAAGTCCAGCAGGCCAACCTAAAAG
  CAATGGGAGATGCTGAAATTAAAGATGGTGAAAGATTGCCACTGGCAGTAAAGGATATGG
  GCAGTTGTGAAATATACCCTCAGACTATTCAGCACAATCCTAATGGGCGGTTTGTGGTGG
  TGTGTGGTGATGGGGAGTATATCATCTACACAGCAATGGCATTGAGAAACAAGAGCTTTG
  GATCTGCTCAGGAGTTTGCATGGGCCCACGATTCTTCAGAGTATGCAATAAGAGAGAGCA
  ACAGCATTGTAAAGATATTTAAGAACTTTAAGGAAAAAAAATCATTTAAACCAGATTTTG
  GAGCAGAAAGTATCTACGGCGGCTTCTTATTGGGAGTCAGATCTGTAAATGGCTTAGCCT
  TCTATGACTGGGACAATACAGAACTCATACGAAGAATTGAAATTCAGCCCAAACATATTT
  TCTGGTCTGACTCTGGAGAGCTAGTCTGTATTGCTACTGAGGAATCATTTTTTATCCTTA
  AGTATCTGTCAGAAAAAGTCTTGGCTGCACAGGAAACACATGAGGGAGTTACTGAAGATG
  GCATTGAAGATGCCTTTGAGGTTCTTGGTGAGATTCAGGAAATTGTGAAAACAGGGCTTT
  GGGTAGGCGATTGCTTCATTTACACAAGTTCTGTGAACAGATTAAATTATTATGTTGGAG
  GAGAAATAGTCACCATTGCCCACTTGGACAGGACGATGTATCTCCTAGGCTACATTCCTA
  AAGACAACAGGCTTTATCTGGGGGATAAAGAATTGAACATCATTAGCTATTCCCTGCTGG
  TTTCAGTCCTGGAATACCAGACAGCTGTCATGCGGAGGGACTTTAGCATGGCTGATAAGG
  TCCTTCCTACCATTCCAAAAGAACAGAGGACCAGAGTTGCACACTTTTTGGAAAAGCAGG
  GCTTCAAGCAGCAAGCTCTTACAGTATCCACAGATCCTGAGCATCGTTTTGAGCTTGCTC
  TTCAGCTTGGAGAGTTAAAAATTGCATACCAGTTAGCAGTGGAAGCAGAGTCAGAACAGA
  AGTGGAAACAACTTGCTGAACTTGCCATTAGTAAATGTCAGTTTGGCCTAGCCCAGGAGT
  GCCTGCATCATGCACAGGATTATGGGGGCCTGCTGCTTTTGGCCACTGCCTCTGGAAATG
  CTAATATGGTGAACAAGCTAGCAGAGGGTGCGGAGAGAGATGGCAAAAATAATGTGGCAT
  TCATGAGCTACTTTTTACAGGGCAAGGTTGATGCCTGCCTAGAGCTCTTAATTAGAACTG
  GACGGCTGCCAGAAGCTGCCTTCTTGGCCCGAACTTACTTACCCAGTCAGGTTTCAAGGG
  TAGTGAAACTCTGGAGAGAGAATCTCTCAAAAGTCAATCAGAAAGCAGCAGAATCCCTTG
  CTGACCCAACAGAGTATGAAAACCTGTTCCCTGGATTAAAAGAAGCCTTTGTTGTTGAAG
  AATGGGTGAAGGAAACACATGCTGATCTGTGGCCAGCCAAACAATACCCACTTGTCACGC
  CAAATGAAGAGAGAAATGTCATGGAAGAGGGAAAAGACTTTCAGCCCTCAAGATCTACAG
  CTCAACAGGAACTTGATGGGAAACCTGCTTCTCCTACTCCGGTTATTGTGGCCTCCCACA
  CAGCCAACAAAGAAGAAAAGAGTTTACTCGAACTAGAAGTAGATTTGGATAATTTGGAAT
  TAGAAGATATTGACACAACAGATATCAATCTGGATGAAGATATTTTGGATGATTGACTGT
  AATGCTTTCCATTTACCTGACTAAACAGATCATTATTATATATAGGTATTGATTGCTACC
  CTGACCACAGTGCTTTGGACTATGAGAAACTTCTTAGATTTTTATATGTAAATGCTGTGG
  ACCACTGGGAGCACAATGCCCACATCATCTTAAGAAGAGTTTATGTGCAGCATTTAAATC
  ACTGTGTTTTCCTTGTTAACTAAAACAGACATGGGCTTTGATTTTTTTCATACTATTAGA
  CCATATCTCATAAAACCTTTTGAATTAATGAAAAAAAAAAAAAAAAAACTCGAC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004766 unedited
  ATACGACTCACTATAGNNGGCGGCCGCGNAATTCGGCACGAGGGTCAGTGGATCGGTGGG
  TTTATCTCAAGGCCTGAGTAGCCGGTAACAAACGAGGGTTCCCGGGATTGGACCGACGCA
  GCCATGCCTCTGCGACTTGATATCAAAAGAAAGCTAACTGCTAGATCTGATCGAGTTAAG
  AGTGTGGATCTGCATCCTACAGAGCCATGGATGTTGGCAAGTCTTTACAATGGCAGTGTG
  TGTGTTTGGAATCATGAAACACAGACACTGGTGAAGACATTTGAAGTATGTGATCTTCCT
  GTTCGAGCTGCAAAGTTTGTTGCAAGGAAGAATTGGGTTGTGACAGGAGCGGATGACATG
  CAGATTAGAGTGTTCAATTACAATACTCTGGAGAGAGTTCATATGTTTGAAGCACACTCA
  GACTACATTCGCTGTATTGCTGTTCATCCAACCCAGCCTTTCATTCTAACTAGCAGTGAT
  GACATGCTTATTAAGCTCTGGGACTGGGATAAAAAATGGTCTTGCTCACAAGTGTTTGAA
  GGACACACCCATTATGTTATGCAGATTGTGATCAACCCCAAAGATAACAATCAGTTTGCC
  AGTGCCTCTTTGGACAGGACTATCAAGGTGTGGCAGTTGGGCTCTTCGTCACCAAACTTC
  ACTTTGGAAGGACATGAGAAAGGCGTGAATTGCATTGATTACTACAGTGGTGGGGACAAG
  CCATACCTCATTTCAGGTGCAGATGACCGTCTTTGTTAAATATGGGATTATCAGAATANA
  ACATGTGTGCAGACACTGGAAGGACATGCCCAAAATGTGTCTTGCGGCAGCTTTCATCCT
  GAGTTGCCAATCATATCACAGGTTCAGAAGATGGACAGTACGTATTTGCATTCAGCACCT
  ACCGGCTGAGAGCACACTGATATGNATGGANAGGTATGTGCGTGCCAGCTAAAGTTCAAC
  ATGTCGCTTGGCTTGATGAGGACTCATGTAGCTGT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004766 unedited
  NCCTTCTTGNACCCGGCCGCATTCTANGATCGAGTTTTTTTTTTTTTTTTTTCATTAATT
  CAAAGGTTTTATGAGATATGGTCTAATAGTATGAAAAAATCAAAGCCCATGTCTGTTTTA
  GTTAACAAGGAAAACACAGTGATTTAAATGCTGCACATAAACTCTTCTTAAGATGATGTG
  GGCATTGTGCTCCCAGTGGTCCACAGCATTTACATATAAAAATCTAAGAAGTTTCTCATA
  GTCCAAAGCACTGTGGTCAGGGTAGCAATCAATACCTATATATAATAATGATCTGTTTAG
  TCAGGTAAATGGAAAGCATTACAGTCAATCATCCAAAATATCTTCATCCAGATTGATATC
  TGTTGTGTCAATATCTTCTAATTCCAAATTATCCAAATCTACTTCTAGTTCGAGTAAACT
  CTTTTCTTCTTTGTTGGCTGTGTGGGAGGCCACAATAACCGGAGTAGGAGAAGCAGGTTT
  CCCATCAAGTTCCTGTTGAGCTGTAGATCTTGAGGGCTGAAAGTCTTTTCCCTCTTCCAT
  GACATTTCTCTCTTCATTTGGCGTGACAAGTGGGTATTGTTTGGCTGGCCACAGATCAGC
  ATGTGTTTCCTTCACCCATTCTTCAACAACAAAGGCTTCTTTTAATCCAGGGAACAGGTT
  TTCATACTCTGTTGGGTCAGCAAGGGATTCTGCTGCTTTCTGATTGACTTTTGAGAGATT
  CTCTCTCCAGAGTTTCACTACCCTTGAAACCTGACTGGGTAAGTAAAGTCGGGCCAAGAA
  GCAGCTTCTGGCAGCCGTCCAGTTCTATTAAGAGCTCTNAGCAGGCATCAACCTTGCCCT
  GTAAAAGTAGCTCATGAATGCCACATTNATTTTGCCATCTCTCTCCGNACCCTCTGCTAG
  CTTGTTCCACATATTAGCATTTCCANAGGCAGTGGCCAAAAA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_004766
• Insert Size: 3000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004766.1, NP_004757.1
• RefSeq Size: 3067 bp
• RefSeq ORF: 2721 bp
• Locus ID: 9276
• UniProt ID: P35606
• Cytogenetics: 3q23
• Domains: WD40, Coatomer_WDAD
• Protein Families: Druggable Genome
• Gene Summary: The Golgi coatomer complex (see MIM 601924) constitutes the coat of nonclathrin-coated vesicles and is essential for Golgi budding and vesicular trafficking. It consists of 7 protein subunits, including COPB2.[supplied by OMIM, Jul 2002]
  Transcript Variant: This variant (1) uses an alternate splice donor site in the first coding exon and is predicted to encode a protein.