ENT1 (SLC29A1) (NM_004955) Human Untagged Clone

SKU SC117050

SLC29A1 (untagged)-Human solute carrier family 29 (nucleoside transporters), member 1 (SLC29A1), nuclear gene encoding mitochondrial protein, transcript variant 5

Product Data

• Type: Human Untagged Clone
• Target Symbol: ENT1
• Synonyms: ENT1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_004955, the custom clone sequence may differ by one or more nucleotides; ; ; ATGACAACCAGTCACCAGCCTCAGGACAGATACAAAGCTGTCTGGCTTATCTTCTTCATGCTGGGTCTGG; GAACGCTGCTCCCGTGGAATTTTTTCATGACGGCCACTCAGTATTTCACAAACCGCCTGGACATGTCCCA; GAATGTGTCCTTGGTCACTGCTGAACTGAGCAAGGACGCCCAGGCGTCAGCCGCCCCTGCAGCACCCTTG; CCTGAGCGGAACTCTCTCAGTGCCATCTTCAACAATGTCATGACCCTATGTGCCATGCTGCCCCTGCTGT; TATTCACCTACCTCAACTCCTTCCTGCATCAGAGGATCCCCCAGTCCGTACGGATCCTGGGCAGCCTGGT; GGCCATCCTGCTGGTGTTTCTGATCACTGCCATCCTGGTGAAGGTGCAGCTGGATGCTCTGCCCTTCTTT; GTCATCACCATGATCAAGATCGTGCTCATTAATTCATTTGGTGCCATCCTGCAGGGCAGCCTGTTTGGTC; TGGCTGGCCTTCTGCCTGCCAGCTACACGGCCCCCATCATGAGTGGCCAGGGCCTAGCAGGCTTCTTTGC; CTCCGTGGCCATGATCTGCGCTATTGCCAGTGGCTCGGAGCTATCAGAAAGTGCCTTCGGCTACTTTATC; ACAGCCTGTGCTGTTATCATTTTGACCATCATCTGTTACCTGGGCCTGCCCCGCCTGGAATTCTACCGCT; ACTACCAGCAGCTCAAGCTTGAAGGACCCGGGGAGCAGGAGACCAAGTTGGACCTCATTAGCAAAGGAGA; GGAGCCAAGAGCAGGCAAAGAGGAATCTGGAGTTTCAGTCTCCAACTCTCAGCCCACCAATGAAAGCCAC; TCTATCAAAGCCATCCTGAAAAATATCTCAGTCCTGGCTTTCTCTGTCTGCTTCATCTTCACTATCACCA; TTGGGATGTTTCCAGCCGTGACTGTTGAGGTCAAGTCCAGCATCGCAGGCAGCAGCACCTGGGAACGTTA; CTTCATTCCTGTGTCCTGTTTCTTGACTTTCAATATCTTTGACTGGTTGGGCCGGAGCCTCACAGCTGTA; TTCATGTGGCCTGGGAAGGACAGCCGCTGGCTGCCAAGCCTGGTGCTGGCCCGGCTGGTGTTTGTGCCAC; TGCTGCTGCTGTGCAACATTAAGCCCCGCCGCTACCTGACTGTGGTCTTCGAGCACGATGCCTGGTTCAT; CTTCTTCATGGCTGCCTTTGCCTTCTCCAACGGCTACCTCGCCAGCCTCTGCATGTGCTTCGGGCCCAAG; AAAGTGAAGCCAGCTGAGGCAGAGACCGCAGGAGCCATCATGGCCTTCTTCCTGTGTCTGGGTCTGGCAC; TGGGGGCTGTTTTCTCCTTCCTGTTCCGGGCAATTGTGTGA; ;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004955 unedited; GCACGAGGCGGGAGAGGGAAGCTGCAGCGAGAGCGCGCGGATCTCAGCGCGGGAGCAGTG; CTTCTGCGGCAGGCCCCTGAGGGAGGGAGCTGTCAGCCAGGGAAAACCGAGAACACCATC; ACCATGACAACCAGTCACCAGCCTCAGGACAGATACAAAGCTGTCTGGCTTATCTTCTTC; ATGCTGGGTCTGGGAACGCTGCTCCCGTGGAATTTTTTCATGACGGCCACTCAGTATTTC; ACAAACCGCCTGGACATGTCCCAGAATGTGTCCTTGGTCACTGCTGAACTGAGCAAGGAC; GCCCAGGCGTCAGCCGCCCCTGCAGCACCCTTGCCTGAGCGGAACTCTCTCAGTGCCATC; TTCAACAATGTCATGACCCTATGTGCCATGCTGCCCCTGCTGTTATTCACCTACCTCAAC; TCCTTCCTGCATCAGAGGATCCCCCAGTCCGTACGGATCCTGGGCAGCCTGGTGGCCATC; CTGCTGGTGTTTCTGATCACTGCCATCCTGGTGAAGGTGCAGCTGGATGCTCTGCCCTTC; TTTGTCATCACCATGATCAAGATCGTGCTCATTAATTCATTTGGTGCCATCCTG;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004955 unedited; GTACCGCGGCACGCAATCTAGTATCGAGTTTTTTTTTTTTTTTTTTGAAAAAAACAAGNA; AGGTTTATTTAAATCAGAGAGCCTCTGAAGGCACCTGGTTTCTGTCAAATATATTTTTAA; AAAAGGCAGTAACGTGGCAACTGTACAGGGTGCATGATGGGTAAGAACTGGGCATGGGGA; GTTGGGAGGGGGACATGGAGAGAACACAGAGGAGGAGGCGCAGGGGAGAATGGAGTATAT; CAGGTCAAACCATACAACGGTCAGACCCAGCTCCTAGCCACCCCAGGACAGGCAGACAGT; CTGACAGACACGGACGCAGACACACAGACACATACACAGAGACACACGTGTTCTCCAAGC; CCAAGAGCCTCCACTGGCCTGCACAAGCAGGGATCAGTCAGAGCCAAGAGTGGAGAAACT; CAAGCAAATGCCGAGGCTACACCCCGTCCCTCGACTCTGAGCCCCAAACCCACAATGTCC; CCACTGTCCGTCCAGAGGCTCCCTCCCCAGGGCCTGGAGATCCGGGCCCAGCACACGCCG; TGGAAAGCAGAAGTCAGTTAGAAGAAAAAACCGCCAGACCACTCAGGATCACCCCTGGCA; GAAGGAAGGGGCAGGAGGCAGACAGGGAGGGAGGCAGGCAGTCCTTCTGTCCATCCTTTG; TCACACAATTGCCCGGAACAGGAATGAGAAAACAGCCCCCAGTGCCAGACCCAGACACAG; GAAGAAAGCCATGATGGCTCCTGCGGTCTCTGCCTCAGCTGCTTCACTTTCTTGGGCCCG; AAACACATGCTTAGCTTGCGATGTATCCGTTTTAGAATGCAAAGCCACCATGAAGAATAT; GACCAGGCTCGTGCTCGAGACCACATCAGTAACGGGGGGCTTAATGTTGACACTCAGATT; GGACAACCCAGTCGGCCATACAG;
• Restriction Sites: NotI-NotI
• ACCN: NM_004955
• Insert Size: 2200 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_004955.1, NP_004946.1
• RefSeq Size: 2341 bp
• RefSeq ORF: 1371 bp
• Locus ID: 2030
• Cytogenetics: 6p21.1
• Domains: Nucleoside_tran
• Protein Families: Transmembrane
• Summary: This gene is a member of the equilibrative nucleoside transporter family. The gene encodes a transmembrane glycoprotein that localizes to the plasma and mitochondrial membranes and mediates the cellular uptake of nucleosides from the surrounding medium. The protein is categorized as an equilibrative (as opposed to concentrative) transporter that is sensitive to inhibition by nitrobenzylthioinosine (NBMPR). Nucleoside transporters are required for nucleotide synthesis in cells that lack de novo nucleoside synthesis pathways, and are also necessary for the uptake of cytotoxic nucleosides used for cancer and viral chemotherapies. Multiple alternatively spliced variants, encoding the same protein, have been found for this gene. [provided by RefSeq, Jul 2008]; Transcript Variant: This variant (5) differs in the 5' UTR compared to variant 1. This variant has an upstream in-frame AUG at nt 255-257. This AUG is not annotated because it has a weak Kozak signal and N-terminal sequencing (PMID 8986748) identified the downstream annotated AUG as the translation start. Variants 1, 2, 3, 4, and 5 encode the same protein.