NDUFB9 (NM_005005) Human Untagged Clone

SKU SC116999

NDUFB9 (untagged)-Human NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 9, 22kDa (NDUFB9), nuclear gene encoding mitochondrial protein

Product Data

• Type: Human Untagged Clone
• Target Symbol: NDUFB9
• Synonyms: B22; CI-B22; LYRM3; MC1DN24; UQOR22
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_005005, the custom clone sequence may differ by one or more nucleotides; ; ; ATGGCGTTCTTGGCGTCGGGACCCTACCTGACCCATCAGCAAAAGGTGTTGCGGCTTTATAAGCGGGCGC; TACGCCACCTCGAGTCGTGGTGCGTCCAGAGAGACAAATACCGATACTTTGCTTGTTTGATGAGAGCCCG; GTTTGAAGAACATAAGAATGAAAAGGATATGGCGAAGGCCACCCAGCTGCTGAAGGAGGCCGAGGAAGAA; TTCTGGTACCGTCAGCATCCACAGCCATACATCTTCCCTGACTCTCCTGGGGGCACCTCCTATGAGAGAT; ACGATTGCTACAAGGTCCCAGAATGGTGCTTAGATGACTGGCATCCTTCTGAGAAGGCAATGTATCCTGA; TTACTTTGCCAAGAGAGAACAGTGGAAGAAACTGCGGAGGGAAAGCTGGGAACGAGAGGTTAAGCAGCTG; CAGGAGGAAACGCCACCTGGTGGTCCTTTAACTGAAGCTTTGCCCCCTGCCCGAAAGGAAGGTGATTTGC; CCCCACTGTGGTGGTATATTGTGACCAGACCCCGGGAGCGGCCCATGTAG; ;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005005 unedited; NGAATGTTCGCATTTTGTNATACGACTCCTATAGGCGGCCGCGNATTCGGCACCAGNAGA; GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA; GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA; GAGAGAGAGGGAAGGTCAGCGCCGTAATGGCGTTCTTGGCGTCGGGACCCTACCTGACCC; ATCAGCAAAAGGTGTTGCGGCTTTATAAGCGGGCGCTACGCCACCTCGAGTCGTGGTGCG; TCCAGAGAGACAAATACCGATACTTTGCTTGTTTGATGAGAGCCCGGTTTGAAGAACATA; AGAATGAAAAGGATATGGCGAAGGCCACCCATCTGCTGAAGGAGGCCGAGGAAGAATTCT; GGTACCGTCAGCATCCACAGCCATACATCTTCCCTGACTCTCCTGGGGGCACCTCCTATG; AGAGATACGATTGCTACAAGGTCCCAGAATGGTGCTTATATGACTGGCATCCTTCTGAGA; AGGCAATGTATCCTGATTACTTTGCCAAGAGAGAACAGTGGAAGAAACTGCGGAGGGAAA; GCTGTGAACGAGAGGTTATGCATCTGCAGGAGGAAACGCCACCTGGTGGTCCTTTAACTG; AAGCTTTGCCCCCTGCCCGAAAGGAAGGTGATTCTGCCCCCACTGTGGTGGTATATTGTG; ACCAGACCCCGCGAGCGGCCCCTGTTTTAAAGAGAGAGACCTCATCTTTTCTGCTTGCCA; GTTGAATATGTTTCAGAACATGCACTTTGCCCTATTTAAAAATCAGTGCAATTGGTCCAC; AAAAACAAAACAATACTCTCTCTTTTTATTGCGGCCGCGGTCTTATTTGTTTTTTTGTCC; A;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005005 unedited; GTACCGCGGCACGCAATCTAGTGTCGAGTTTTTTTTTTTTTTTTTTTGACCATTTCACTG; ATTTTTTATTAAGGCAAGTGCATGTTCTGTAACATATTTCACTTGCAAGCATGAAAGATG; AAGTCTCTCTCTTTCTACATGGGCCGCTCCCGGGGTCTGGTCACAATATACCACCACAGT; GGGGGCAAATCACCTTCCTTTCGGGCAGGGGGCAAAGCTTCAGTTAAAGGACCACCAGGT; GGCGTTTCCTCCTGCAGCTGCTTAACCTCTCGTTCCCAGCTTTCCCTCCGCAGTTTCTTC; CACTGTTCTCTCTTGGCAAAGTAATCAGGATACATTGCCTTCTCAGAAAGATGCCAGTCA; TCTAAGCACCATTCTGGGACCTTGTAGCAATCGTATCTCTCATATGAAGTGCCCCCAGGA; GAGTCAAGGAAGATGTATGGCTGTGGATGCTGACGGTACCAAAATTCTTCCTCGGCCTCC; TTCAGCAGCTGGGGGGCCCTCGCCAAAACTTTTTATTCTTATGCCTTCAACCGGCCTCTC; TAAAAAACAAAGATCGGCATTTGCCTTCTGGACCCCCACAACTGAGGGGCGTACGCCCCT; ATAATACACAACACCTTTCCTGCGGGCACGCACGCCCCACTCCAAACACCACACGGCCCC; CACCCCCCCGTCTCTCTTCCCGCCCCCCCCCCCCCCCCCGTTCACACGGACAAAACTACC; CGCCGAATCCTCACCCCCACCCCCTCACACGCGACTAATTCCCCGTCCCACTCGCCGCGC; GCCACGACCAGCCACAACAGTGACCTAATCTCTTCTCAGCCACCCCATCTCCCACCTACC; CGGATCCACCCGATCCACCATATACTATCTCCACCCCCCACATACACCACACACCCACCA; ACACACTACAGCAGATCTCCTCATCCTCTCTGCAACACACCCCACCATCTCTACTCGCCG; TTTCCCTATCTCCTTTCCCG;
• Restriction Sites: NotI-NotI
• ACCN: NM_005005
• Insert Size: 860 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_005005.1, NP_004996.1
• RefSeq Size: 740 bp
• RefSeq ORF: 540 bp
• Locus ID: 4715
• UniProt ID: Q9Y6M9
• Cytogenetics: 8q24.13
• Protein Pathways: Alzheimer's disease, Huntington's disease, Metabolic pathways, Oxidative phosphorylation, Parkinson's disease
• Summary: The protein encoded by this gene is a subunit of the mitochondrial oxidative phosphorylation complex I (nicotinamide adenine dinucleotide: ubiquinone oxidoreductase). Complex I is localized to the inner mitochondrial membrane and functions to dehydrogenate nicotinamide adenine dinucleotide and to shuttle electrons to coenzyme Q. Complex I deficiency is the most common defect found in oxidative phosphorylation disorders and results in a range of conditions, including lethal neonatal disease, hypertrophic cardiomyopathy, liver disease, and adult-onset neurodegenerative disorders. Pseudogenes of this gene are found on chromosomes five, seven and eight. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2015]; Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).