Y14 (RBM8A) (NM_005105) Human Untagged Clone

SKU SC116929

RBM8A (untagged)-Human RNA binding motif protein 8A (RBM8A)

Product Data

• Type: Human Untagged Clone
• Target Symbol: Y14
• Synonyms: BOV-1A; BOV-1B; BOV-1C; C1DELq21.1; DEL1q21.1; MDS014; RBM8; RBM8B; TAR; Y14; ZNRP; ZRNP1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_005105, the custom clone sequence may differ by one or more nucleotides; ; ; ATGGCGGACGTGCTAGATCTTCACGAGGCTGGGGGCGAAGATTTCGCCATGGATGAGGATGGGGACGAGA; GCATTCACAAACTGAAAGAAAAAGCGAAGAAACGGAAGGGTCGCGGCTTTGGCTCCGAAGAGGGGTCCCG; AGCGCGGATGCGTGAGGATTATGACAGCGTGGAGCAGGATGGCGATGAACCCGGACCACAACGCTCTGTT; GAAGGCTGGATTCTCTTTGTAACTGGAGTCCATGAGGAAGCCACCGAAGAAGACATACACGACAAATTCG; CAGAATATGGGGAAATTAAAAACATTCATCTCAACCTCGACAGGCGAACAGGATATCTGAAGGGGTATAC; TCTAGTTGAATATGAAACATACAAGGAAGCCCAGGCTGCTATGGAGGGACTCAATGGCCAGGATTTGATG; GGACAGCCCATCAGCGTTGACTGGTGTTTTGTTCGGGGTCCACCAAAAGGCAAGAGGAGAGGTGGCCGAA; GACGCAGCAGAAGTCCAGACCGGAGACGTCGCTGA; ;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005105 unedited; NGGTTCANAATTTGTAATACGACTTACTATAGGGCGGCCGCGAAATTCGCACGAGGGCGA; GATGGCGGACGTGCTAGATCTTCACGAGGCTGGGGGCGAAGATTTCGCCATGGATGAGGA; TGGGGACGAGAGCATTCACAAACTGAAAGAAAAAGCGAAGAAACGGAAGGGTCGCGGCTT; TGGCTCCGAAGAGGGGTCCCGAGCGCGGATGCGTGAGGATTATGACAGCGTGGAGCAGGA; TGGCGATGAACCCGGACCACAACGCTCTGTTGAAGGCTGGATTCTCTTTGTAACTGGAGT; CCATGAGGAAGCCACCGAAGAAGACATACACGACAAATTCGCAGAATATGGGGAAATTAA; AAACATTCATCTCAACCTCGACAGGCGAACAGGATATCTGAAGGGGTATACTCTAGTTGA; ATATGAAACATACAAGGAAGCCCAGGCTGCTATGGAGGGACTCAATGGCCAGGATTTGAT; GGGACAGCCCATCAGCGTTGACTGGTGTTTTGTTCGGGGTCCACCAAAAGGCAAGAGGAG; AGGTGGCCGAAGACGCAGCAGAAGTCCAGACCGGAGACGTCGCTGACAGGTCCTCTGTTG; TCCAGGTGTTCTCTTCAAGATTCCATTTGACCATGCAGCCTTGGACAAATAGGACTGNGG; TGGAACTTGCTGTGTTTATATTTAATCTCTTACCGTATATGCGTAGTATTTGAGTTGCGA; ATAAATGTTCCATTTTTGTTNTCTACATTTAATGTTACTTTCCTGTCCTAAAATGAAAAG; TCTAAAGCATAGCAGGNCTGTATGGATCATTGTGAAGATACTTCTAGGNACTGAACTCTA; TGNATTNCTTTNTTTTTCTTNTTNTGAGAAGAGTCTTGCTGTGTTACCCANGNNTGNATG; CAGCTGATATAGCTACTGCAGCTTCAACTCTGGGCTCAAGCATNCTCTGNCTACTGG;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005105 unedited; NNTTTTAGCTTTGNACCCGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTGG; CATTTGGAAAATGTTAAATTTTATTATTTAACATTCTTTACCATTATAGTTACTGCACAT; AAGACTATTACTACTAAAGGTCACTTCAGAGTCCCTGCAAAATGGCCTGGAATTTTGGCA; GCACCCATTTTACACAATATTTCTTTTTCCACAAAATAACAGACATACCAGGAAAATCAT; TTCAGCTAAAAATATGAGTGAGGTGGTAGAAATATCATCCCTTATAAAGCGCAATGTTAG; AATAGTACTTGAGAAAGCAGGATTGTTTTAAGTTCCAAGATTTAACAAACTTACTGTTCA; GCATCATATTCAAGCCTAAAAGGAAGATAGGATTTTCAAGATATATTTCCAACTTCTTTA; ACATGGCACCATGGATGAACTGTTTCTCAGCACTGTGCTGCTTCACTTGGAATTAAGGAT; GAATTGGGAGGAGACAGTATGACATAGGTGGGTAGGTTGGGTGGTGAGGGGAACCAGTTC; TAATAGTCCTCAACTCCACTCCAGCTGTTCCTGTTCCACACGGTCCACTGAGCTGGCCCA; GTCCCTTTCACTCAGTGTGTCACCAAAGGCAGCTTCAAGGCTCAATGGCAAGAGACCACC; TATAACCTCTTCACCTTCTGCTGCCTCTTTCTGCTGCCACTGACTGCCATGGCCATCTGC; TATAGCCGCATTGTCCTCAGTGTGTCCAGGCCCCAGACAGGNAAGGNGAGCCATGGTGAG; ACTNCAATTCCCAGCCCTAATCCTTAAAACCTAGACTGTGNCTCTAGCATCATTTANTTA; TCTACCTACCCTATAGCTATCTACCAGTCACTAAACCATGGTGAGAATCTACCATGTCTA; GCACCTGAGCTAGAGATAATTTTGNTGAATCCCTTCCATATAACAGCTGAGGTAGCTA;
• Restriction Sites: NotI-NotI
• ACCN: NM_005105
• Insert Size: 2880 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_005105.2, NP_005096.1
• RefSeq Size: 2787 bp
• RefSeq ORF: 525 bp
• Locus ID: 9939
• UniProt ID: Q9Y5S9
• Cytogenetics: 1q21.1
• Domains: RRM
• Protein Families: Druggable Genome
• Protein Pathways: Spliceosome
• Summary: This gene encodes a protein with a conserved RNA-binding motif. The protein is found predominantly in the nucleus, although it is also present in the cytoplasm. It is preferentially associated with mRNAs produced by splicing, including both nuclear mRNAs and newly exported cytoplasmic mRNAs. It is thought that the protein remains associated with spliced mRNAs as a tag to indicate where introns had been present, thus coupling pre- and post-mRNA splicing events. Previously, it was thought that two genes encode this protein, RBM8A and RBM8B; it is now thought that the RBM8B locus is a pseudogene. There are two alternate translation start codons with this gene, which result in two forms of the protein. An allele mutation and a low-frequency noncoding single-nucleotide polymorphism (SNP) in this gene cause thrombocytopenia-absent radius (TAR) syndrome. [provided by RefSeq, Jul 2013]