Calpain 1 (CAPN1) (NM_005186) Human Untagged Clone

SKU SC116897

CAPN1 (untagged)-Human calpain 1, (mu/I) large subunit (CAPN1), transcript variant 2

Product Data

• Type: Human Untagged Clone
• Target Symbol: Calpain 1
• Synonyms: CANP; CANP1; CANPL1; muCANP; muCL; SPG76
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_005186 edited; GCGGCCGCGAATTCGGCACGAGGGGGAAGCCAGCCTCAGGGACTGCAGCGACCCCCAAAC; ACCCCTCCCCCAGATGTCGGAGGAGATCATCACGCCGGTGTACTGCACTGGGGTGTCAGC; CCAAGTGCAGAAGCAGCGGGCCAGGGAGCTGGGCCTGGGCCGCCATGAGAATGCCATCAA; GTACCTGGGCCAGGATTATGAGCAGCTGCGGGTGCGATGCCTGCAGAGTGGGACCCTCTT; CCGTGATGAGGCCTTCCCCCCGGTACCCCAGAGCCTGGGTTACAAGGACCTGGGTCCCAA; TTCCTCCAAGACCTATGGCATCAAGTGGAAGCGTCCCACGGAACTGCTGTCAAACCCCCA; GTTCATTGTGGATGGAGCTACCCGCACAGACATCTGCCAGGGAGCACTGGGGGACTGCTG; GCTCTTGGCGGCCATCGCCTCCCTCACTCTCAACGACACCCTCCTGCACCGAGTGGTTCC; GCACGGCCAGAGCTTCCAGAATGGCTATGCCGGCATCTTCCATTTCCAGCTGTGGCAATT; TGGGGAGTGGGTGGACGTGGTCGTGGATGACCTGCTGCCCATCAAGGACGGGAAGCTAGT; GTTCGTGCACTCTGCCGAAGGCAACGAGTTCTGGAGCGCCCTGCTTGAGAAGGCCTATGC; CAAGGTAAATGGCAGCTACGAGGCCCTGTCAGGGGGCAGCACCTCAGAGGGCTTTGAGGA; CTTCACAGGCGGGGTTACCGAGTGGTACGAGTTGCGCAAGGCTCCCAGTGACCTCTACCA; GATCATCCTCAAGGCGCTGGAGCGGGGCTCCCTGCTGGGCTGCTCCATAGACATCTCCAG; CGTTCTAGACATGGAGGCCATCACTTTCAAGAAGTTGGTGAAGGGCCATGCCTACTCTGT; GACCGGGGCCAAGCAGGTGAACTACCGAGGCCAGGTGGTGAGCCTGATCCGGATGCGGAA; CCCCTGGGGCGAGGTGGAGTGGACGGGAGCCTGGAGCGACAGCTCCTCAGAGTGGAACAA; CGTGGACCCATATGAACGGGACCAGCTCCGGGTCAAGATGGAGGACGGGGAGTTCTGGAT; GTCATTCCGAGACTTCATGCGGGAGTTCACCCGCCTGGAGATCTGCAACCTCACACCCGA; CGCCCTCAAGAGCCGGACCATCCGCAAATGGAACACCACACTCTACGAAGGCACCTGGCG; GCGGGGGAGCACCGCGGGGGGCTGCCGAAACTACCCAGCCACCTTCTGGGTGAACCCTCA; GTTCAAGATCCGGCTGGATGAGACGGATGACCCGGACGACTACGGGGACCGCGAGTCAGG; CTGCAGCTTCGTGCTCGCCCTTATGCAGAAGCACCGTCGCCGCGAGCGCCCCTTCGGCCG; CGACATGGAGACTATTGGCTTCGCGGTCTACGAGGTCCCTCCGGAGCTGGTGGGCCAGCC; GGCCGTACACTTGAAGCGTGACTTCTTCCTGGCCAATGCGTCTCGGGCGCGCTCAGAGCA; GTTCATCAACCTGCGAGAGGTCAGCACCCGCTTCCGCCTGCCACCCGGGGAGTATGTGGT; GGTGCCCTCCACCTTCGAGCCCAACAAGGAGGGCGACTTCGTGCTGCGCTTCTTCTCAGA; GAAGAGTGCTGGGACTGTGGAGCTGGATGACCAGATCCAGGCCAATCTCCCCGATGAGCA; AGTGCTCTCAGAAGAGGAGATTGACGAGAACTTCAAGGCCCTCTTCAGGCAGCTGGCAGG; GGAGGACATGGAGATCAGCGTGAAGGAGTTGCGGACAATCCTCAATAGGATCATCAGCAA; ACACAAAGACCTGCGGACCAAGGGCTTCAGCCTAGAGTCGTGCCGCAGCATGGTGAACCT; CATGGATCGTGATGGCAATGGGAAGCTGGGCCTGGTGGAGTTCAACATCCTGTGGAACCG; CATCCGGAATTACCTGTCCATCTTCCGGAAGTTTGACCTGGACAAGTCGGGCAGCATGAG; TGCCTACGAGATGCGGATGGCCATTGAGTCGGCAGGCTTCAAGCTCAACAAGAAGCTGTA; CGAGCTCATCATCACCCGCTACTCGGAGCCCGACCTGGCGGTCGACTTTGACAATTTCGT; TTGCTGCCTGGTGCGGCTAGAGACCATGTTCCGATTTTTCAAAACTCTGGACACAGATCT; GGATGGAGTTGTGACCTTTGACTTGTTTAAGTGGTTGCAGCTGACCATGTTTGCATGAGG; CAGGGACTCGGTCCCCCTTGCCGTGCTCCCCTCCCTCCTCGTCTGCCAAGCCTCGCCTCC; TACCACACCACACCAGGCCACCCCAGCTGCAAGTGCCTTCCTTGGAGCAGAGAGGCAGCC; TCGTCCTCCTGTCCCCTCTCCTCCCAGCCACCATCGTTCATCTGCTCCGGGCAGAACTGT; GTGGCCCCTGCCTGTGCCAGCCATGGGCTCGGGATGGACTCCCTGGGCCCCACCCATTGC; CAAGCCAGGAAGGCAGCTTTCGCTTGTTCCTGCCTCGGGACAGCCCCGGGTTTCCCCAGC; ATCCTGATGTGTCCCCTCTCCCCACTTCAGAGGCCACCCACTCAGCACCACCGGCCTGGC; CTTGCCTGCAGACTATAAACTATAACCACTAGCTCGACACAGTCTGCAGTCCAGGCGTGT; GGAGCCGCCTCCCGGCTCGGGGAGGCCCCGGGGCTGGGAACGCCTGTGCCTTCCTGCGCC; GAAGCCAACGCCCCCTCTGTCCTTCCCTGGCCCTGCTGCCGACCAGGAGCTGCCCAGCCT; GTGGGCGGTCGGCCTTCCCTCCTTCGCTCCTTTTTTATATTAGTGATTTTAAAGGGGACT; CTTCAGGGACTTGTGTACTGGTTATGGGGGTGCCAGAGGCACTAGGCTTGGGGTGGGGAG; GTTCCCGTGTTCCATATAGAGGAACCCCAAATAA;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005186 unedited; ACTAGNGATTTTGAAATACCCACTTTCTATGAGGGCGGCACAAAACTTCGGCACGAGGGG; GAAGCCAGCCTCAGGGACTGCAGCGACCCCCAAACACCCCTCCCCCAGATGTCGGAGGAG; ATCATCACGCCGGTGTACTGCACTGGGGTGTCAGCCCAAGTGCAGAAGCAGCGGGCCAGG; GAGCTGGGCCTGGGCCGCCATGAGAATGCCATCAAGTACTTGTTCCAGGATTATGAGCAG; CTGCGGGTGCGATGCCTGCAGAGTGGGACCCTCTTCCGTGATGAGGCCTTCCCCCCGGTA; CCCCAGAGCCTGGGTTACAAGGACCTGGGTCCCAATTCCTCCAAGACCTATGGCATCAAG; TGGAAGCGTCCCACGGAACTGCTGTCAAACCCCCAGTTCATTGTGGATGGAGCTACCCGC; ACAGACATCTGCCAGGGAGCACTGGGGGACTGCTGGCTCTTGGCGGCCATCGCCTCCCTC; ACTCTCAACGACACCCTCCTGCACCGAGTGGTTCCGCACGGCCAGAGCTTCCAGAATGGC; TATGCCGGCATCTTCCATTTCCAGCTGTGGCAATTTGGGGAGTGGGTGGACGTGGTCGTG; GATGACCTGCTGCCCATCAAGGACGGGAAGCTAGTGTTCGTGCACTCTGCCGAAGCAACG; AGTTCTGGAGCGCCCTGCTTGAGAAGGCCTATGCCAAGGTAAATGGCAGCTACGAAGCCC; TGTCAGGGGGCAGCACCTCAGAGGGCTTTGAGGACTTCACAGGCGGNNGTACCGAGTGGT; ACGAGTTGCGCAAGGCTNCCAGTGACCTCCTACAGATCATCCTCAAGGCGCTTGGAGCGG; GGCTCCCTGCTGGGCTGGCTCATAGACATCTCCAGCGTTCTAAGAATGGAGGCCATCACT; TTCAAAGAGTTGGTTGAAGGCCAATGCTTACTCTGTGACCGGGGCAAACAG;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005186 unedited; CCCCCCAATACTGTGCACCGCGGCCGCATCTNACGAGCGGTTTTTTTTTTTTTTTTTTCA; CAGCACGAAGTGGGGCCCTTTTATTATTTGGGGTTCCTCTATATGGAACACGGGAACCTC; CCCACCCCAAGCCTAGTGCCTCTGGCACCCCCATAACCAGTACACAAGTCCCTGAAGAGT; CCCCTTTAAAATCACTAATATAAAAAAGGAGCGAAGGAGGGAAGGCCGACCGCCCACAGG; CTGGGCAGCTCCTGGTCGGCAGCAGGGCCAGGGAAGGACAGAGGGGGCGTTGGCTTCGGC; GCAGGAAGGCACAGGCGTTCCCAGCCCCGGGGCCTCCCCGAGCCGGGAGGCGGCTCCACA; CGCCTGGACTGCAAACTGTGTCGAGCTAGTGGTTATAGTTTATAGTCTGCAGGCAAGGCC; AGGCCGGTGGTGCTGAGTGGGTGGCCTCTGAAGTGGGGAGAGGGGACACATCAGGATGCT; GGGGAAACCCGGGGCTGTCCCGAGGCAGGAACAAGCGAAAGCTGCCTTCCTGGCTTGGCA; ATGGGTGGGGCCCAGGGAGTCCATCCCGAGCCCATGGCTGGCACAGGCAGGGGCCACACA; GTTCTGCCCGGAGCAGATGAACGATGGTGGCTGGGAGGAGAGGGGACAGGAGGACAAGGC; TGCCTCTCTGCTCCAAGGAAGGCACTTGCAGCTGGGGTGGCCTGCTGTGGTGTGGTAGGA; GGCCAGGCTTGGCACACTAGGAGGGAGGGGAGCACGGCAGGGGGACCGAGTCCTGCCTCA; TGCAAACATGTTCAGCTGCAACCCTTAAACAAGTCAAAGTCACAACTCCATCCAGATCTG; TGTCCACAGTTTTTGAAAATCGAACATGGTCTTTACCGCACCCAGCAGCAACGAAATGTC; AAGTCGACG;
• Restriction Sites: NotI-NotI
• ACCN: NM_005186
• Insert Size: 2880 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_005186.2, NP_005177.2
• RefSeq Size: 3007 bp
• RefSeq ORF: 2145 bp
• Locus ID: 823
• UniProt ID: P07384
• Cytogenetics: 11q13.1
• Domains: Calpain_III, EFh
• Protein Families: Druggable Genome, Protease
• Protein Pathways: Alzheimer's disease, Apoptosis
• Summary: The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 1. Several transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Nov 2010]; Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1, 2, and 3 all encode the same protein.