APLP1 (NM_005166) Human Untagged Clone

CAT#: SC116888

APLP1 (untagged)-Human amyloid beta (A4) precursor-like protein 1 (APLP1), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: APLP1
• Synonyms: APLP
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC116888 sequence for NM_005166 edited (data generated by NextGen Sequencing)
  ATGGGGCCCGCCAGCCCCGCTGCTCGCGGTCTAAGTCGCCGCCCGGGCCAGCCGCCGCTG
  CCGCTGCTGCTGCCACTATTGCTGCTGCTTCTGCGCGCGCAGCCCGCCATCGGGAGCCTG
  GCCGGTGGGAGCCCCGGCGCGGCCGAGGCCCCGGGGTCGGCCCAGGTGGCTGGACTATGC
  GGGCGCCTAACCCTTCACCGGGACCTGCGCACCGGCCGCTGGGAACCAGACCCACAGCGC
  TCTCGACGCTGTCTCCGGGACCCGCAGCGCGTGCTGGAGTACTGCAGACAGATGTACCCG
  GAGCTGCAGATTGCACGTGTGGAGCAGGCTACGCAGGCCATCCCCATGGAGCGCTGGTGC
  GGGGGTTCCCGGAGCGGCAGCTGCGCCCACCCCCACCACCAGGTTGTGCCCTTCCGCTGC
  CTGCCTGGTGAATTTGTGAGTGAGGCCCTGCTGGTGCCTGAAGGCTGCCGGTTCTTGCAC
  CAGGAGCGCATGGACCAATGTGAGAGTTCAACCCGGAGGCATCAGGAGGCACAGGAGGCC
  TGCAGCTCCCAGGGCCTCATCCTGCACGGCTCGGGCATGCTCTTACCCTGTGGCTCGGAT
  CGGTTCCGTGGTGTGGAGTATGTGTGCTGTCCCCCTCCAGGGACCCCCGACCCATCTGGG
  ACAGCAGTTGGTGACCCCTCCACCCGGTCCTGGCCCCCGGGGAGCAGAGTAGAGGGGGCT
  GAGGACGAGGAAGAGGAGGAATCCTTCCCACAGCCAGTAGATGATTACTTCGTGGAGCCT
  CCGCAGGCTGAAGAGGAAGAGGAAACGGTCCCACCCCCAAGCTCCCATACACTTGCAGTG
  GTCGGCAAAGTCACTCCCACCCCGAGGCCCACAGACGGTGTGGATATTTACTTTGGCATG
  CCTGGGGAAATCAGTGAGCACGAGGGGTTCCTGAGGGCCAAGATGGACCTGGAGGAGCGT
  AGGATGCGCCAGATTAATGAGGTGATGCGTGAATGGGCCATGGCAGACAACCAGTCCAAG
  AACCTGCCTAAAGCCGACAGACAGGCCCTGAATGAGCACTTCCAGTCCATTCTGCAGACT
  CTGGAGGAGCAGGTGTCTGGTGAGCGACAGCGCCTGGTGGAAACCCACGCCACCCGCGTC
  ATCGCCCTTATCAACGACCAGCGCCGGGCTGCCTTGGAGGGCTTCCTGGCAGCCCTGCAG
  GCAGATCCGCCTCAGGCGGAGCGTGTCCTGTTGGCCCTGCGGCGCTACCTGCGTGCGGAG
  CAGAAGGAACAGAGGCACACGCTGCGCCACTACCAGCATGTGGCCGCCGTGGATCCCGAG
  AAGGCACAGCAGATGCGCTTCCAGGTGCATACCCACCTTCAAGTGATTGAGGAGAGGGTG
  AATCAGAGCCTGGGCCTGCTTGACCAGAACCCCCACCTGGCTCAGGAGCTGCGGCCCCAA
  ATCCAGGAACTCCTCCACTCTGAACACCTGGGTCCCAGTGAATTGGAAGCCCCTGCCCCT
  GGGGGCAGCAGCGAGGACAAGGGTGGGCTGCAGCCTCCAGATTCCAAGGATGACACCCCC
  ATGACCCTTCCAAAAGGGTCCACAGAACAAGATGCTGCATCCCCTGAGAAAGAGAAGATG
  AACCCGCTGGAACAGTATGAGCGAAAGGTGAATGCGTCTGTTCCAAGGGGTTTCCCTTTC
  CACTCATCGGAGATTCAGAGGGATGAGCTGGCACCAGCTGGGACAGGGGTGTCCCGTGAG
  GCTGTGTCGGGTCTGCTGATCATGGGAGCGGGCGGAGGCTCCCTCATCGTCCTCTCCATG
  CTGCTCCTGCGCAGGAAGAAGCCCTACGGGGCTATCAGCCATGGCGTGGTGGAGGTGGAC
  CCCATGCTGACCCTGGAGGAGCAGCAGCTCCGCGAACTGCAGCGGCACGGCTATGAGAAC
  CCCACTTACCGCTTCCTGGAGGAACGACCCTGA
  
  Clone variation with respect to NM_005166.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005166 unedited
  CCGCACTTCTATAGGCGGCCGCGCAATTCGCACGAGGCATGGGGCCCGCCAGCCCCGCTG
  CTCGCGGTCTAAGGGGGCGCCCGGGCCAGCCGCCGCTGCCGCTGCTGCTGCCACTATTGC
  TGCTGCTTCTGCGCGCGCAGCCCGCCATCGGGAGCCTGGCCGGTGGGAGCCCCGGCGCGG
  CCGAGGCCCCGGGGTCGGCCCAGGTGGCTGGACTATGCGGGCGCCTAACCCTTCACCGGG
  ACCTGCGCACCGGCCGCTGGGAACCAGACCCACAGCGCTCTCGACGCTGTCTCCGGGACC
  CGCAGCGCGTGCTGGAGTACTGCAGACAGATGTACCCGGAGCTGCAGATTGCACGTGTGG
  AGCAGGCTACGCAGGCCATCCCCATGGAGCGCTGGTGCGGGGGTTCCCGGAGCGGCAGCT
  GCGCCCACCCCCACCACCAGGTTGTGCCCTTCCGCTGCCTGCCTGGTGAATTTGTGAGTG
  AGGCCCTGCTGGTGCCTGAAGGCTGCCGGTTCTTGCACCAGGAGCGCATGGACCAATGTG
  AGAGTTCAACCCGGAGGCATCAGGAGGCACAGGAGGCCTGCAGCTCCCAGGGCCTCATCC
  TGCACGGCTCGGGCATGCTCTTACCCTGTGGCTCGGATCGGTTCCGTGGTGTGGAGTATG
  TGTGCTGTCCCCCTCCAGGGACCCCCGACCCATCTGGGACAGCAGTTGGTGACCCCTCCA
  CCCGTCCTGGCCCCCGGNGAGCANAGTANAGGGGGCTGANGACGANGAAGAGGAGAATCC
  TTCCACAGCCAGTAGATGATACTTCGTGGAGCCTCCGCAGCTGAAAAGAGAGGGNAAACG
  GTCCCACCCCAGCTCCATACCTNGCAGTGGTCGGNAAGTCACTCCACCCGAGCCACAGAA
  GGGGGTGAATATTACTTTGGCTGCCTGGGAATCATGGACCCAGGGGTCCTGAGGGCANAT
  GGACTNAAGGACCTAGAGCCCT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005166 unedited
  ACTTCCAGGGCCGGNAAAGCACTGGGGAGGGGTCACAGGGATGCCACCCGGGATCTGTTC
  AGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGGT
  AGGGAAGAGGACTTTATTGGGATGTTAGTAGGGAAACATGAGAGGGTGAATTCCAGGGAA
  TAGACACTAGGACCAAGGTGGCGGTCACCTTAAAGAGCCATAAATAAACTTAAAAAATAA
  ATTAAGGTGAGGAGGTGCCACGTGGGGAGGCTGCTGGGACTATCTGGGAATTCTTAGGGA
  TGGAATTTTGGAATTGGAAAGGGGAAATAAGAATTTCCAGCCGTCTCACAAAAGGGTGTG
  AAATGATCACTTCAAGACTCCCTGCTGCCCTAGGCTGGGAGTTGGGGTTCTGGGGCTCCA
  GGAAGAGGGGAGGTCTGGGCTCGGCTGAAGGGGTGAAGGGGGCCGGGTCAGGGTCGTTCC
  TCCAGGAAGCGGTAAGTGGGGTTCTCATAGCCGTGCCGCTGCAGTTCGCGGAGCTGCTGC
  TCCTCCAGGGTCAGCATGGGGTCCACCTCCACCACGCCATGGCTGATAGCCCCGTNAGGC
  TTCTTCCTGCGCAGGAGCAGCATGGAGAGGACGATGAGGGAGCCTCCGCCCGCTCCCATG
  ATCAGCAGACCCGACACAGCCTCACGGGACACCCCTGTCCCAGCTGGTGCCAGCTCATCC
  CTCTGAATCTNCGATGAGTGGGAAAGGGAAACCCCTTGGAACAGACGCATTCACCTTTCG
  CTATACTGGTCCAGCGGGTTCATCTTCTCTTCTCAGGGGATGCGCATCTTGTTCTGTGAC
  CTTTNGGAGGTAATNGGGGGGGGGTCATCTTGATCTGGAGCTGCACCCACCTTGNCTCCT
  GTGCCCAGGGGGAGGGGCTTCATACTGAACCAGTGTGTTAANGGAAAGGGAGCTTTGGTT
  TTTTGGGCGATCTCACACAGNNGNNGGNNTTNT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005166
• Insert Size: 2440 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005166.3, NP_005157.1
• RefSeq Size: 2447 bp
• RefSeq ORF: 1953 bp
• Locus ID: 333
• UniProt ID: P51693
• Cytogenetics: 19q13.12
• Domains: A4_EXTRA
• Protein Families: Druggable Genome, Transmembrane
• Gene Summary: This gene encodes a member of the highly conserved amyloid precursor protein gene family. The encoded protein is a membrane-associated glycoprotein that is cleaved by secretases in a manner similar to amyloid beta A4 precursor protein cleavage. This cleavage liberates an intracellular cytoplasmic fragment that may act as a transcriptional activator. The encoded protein may also play a role in synaptic maturation during cortical development. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (2) uses an alternate in-frame splice site in the coding region, compared to variant 1. It encodes isoform 2, which is shorter than isoform 1.