beta Defensin 1 (DEFB1) (NM_005218) Human Untagged Clone

SKU SC116851

DEFB1 (untagged)-Human defensin, beta 1 (DEFB1)

Product Data

• Type: Human Untagged Clone
• Target Symbol: beta Defensin 1
• Synonyms: BD1; DEFB-1; DEFB101; HBD1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_005218 edited; ATGAGAACTTCCTACCTTCTGCTGTTTACTCTCTGCTTACTTTTGTCTGAGATGGCCTCA; GGTGGTAACTTTCTCACAGGCCTTGGCCACAGATCTGATCATTACAATTGCGTCAGCAGT; GGAGGGCAATGTCTCTATTCTGCCTGCCCGATCTTTACCAAAATTCAAGGCACCTGTTAC; AGAGGGAAGGCCAAGTGCTGCAAGTGA;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005218 unedited; CCGCGAATTCGGCACGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG; AGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG; AGAGAGAGAGAGAGAGAGAGCTCAGCCTCCAAAGAAGCCAGCCTCTCCCCAGTTCCTGAA; ATCCTGGGTGTTGCCTGCCAGTCGCCATGAGAACTTCCTACCTTCTGCTGTTTACTCTCT; GCTTACTTTTGTCTGAGATGGCCTCAGGTGGTAACTTTCTCACAGGCCTTGGCCACAGAT; CTGATCATTACAATTGCGTCAGCAGTGGAGGGCAATGTCTCTATTCTGCCTGCCCGATCT; TTACCAAAATTCAAGGCACCTGTTACAGAGGGAAGGCCAAGTGCTGCAAGTGAGCTGGGA; GTGACCAGAAGAAATGACGCAGAAGTGAAATGAACTTTTTATAAGCATTCTTTTAATAAA; GGAAAATTGCTTTTGAAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACTCGACTCTAG; ATTGCGGCCGCGGTCATAGCTGTTTCCTGAACAGATCCCGGGTGGCATCCCTGTGACCCC; TCCCCAGTGCCTCTCCTGGGCCCTGGAAGTTGCCACTCCAGTGCCCACCAGCCTTGTCCT; AATAAATTAAGTTGCATCATTTTGTCTGACTAGGTGTCCTCTATATATATGGGAGGGGGG; GGGGGNTTNNNNNNNAAACGGGGGGCGTGGTGGAAACG;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005218 unedited; TACCGCAGGCTGNACTTAGATCGAGTAATATATCTTTTTTTTTTTTTTTTTTTTTACTTC; AAAAGCAATTTTCCTTTATTAAAAGAATGCTTATAAAAAGTTCATTTCACTTCTGCGTCA; TTTCTTCTGGTCACTCCCAGCTCACTTGCAGCACTTGGCCTTCCCTCTGTAACAGGGGCC; TTGAATTTTGGTAAAGATCGGGCAGGCAGAATAGAAACATTGCCCTCCACTGCTGACGCA; ATTGTAATGATCAGATCTGTGGCCAAGGCCTGTGAAAAAGTTACCACCTGAGGCCATCTC; AGACAAAAGTAAGCAGAGAGTAAACAGCAGAAGGTAGGAAGTTCTCATGGCGACTGGCAG; GCAACACCCAGGATTTCAGGAACTGGGGAGAGGCTGGCTTCTTTGGAGGCTGAGCTCTCT; CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTNCCT; TTCCCCCTCCCCCCTTTCTTTTTTTTTTCTTTTTTTTTCTCCCCCTCCCTCTTTTTNCTC; TTTCCCTTCCTCCCCCCCTNTTCNCCCTCCCCCCTCCCCCCCCCCTTCTTCCTTCTTCTT; TCTTTCTTCCTTCCCCCCCNTTTCCTCCCNNCTCCCCTCTCCCTTTTCTCCCCTCCCCCC; CCCCTCCTTCCCCTTTCCCCTTTCTCCCTCCCCCCTCCTTCTTCTTCCCCCTCCCCCCTC; CTTTTTTCTCCCCCTCTTTCCCCCCTTCCTTCCCCCCTCCCTTCCCCTCCTTTTCCCTTC; TTCCCTCCCNCCCCCCCTTCCCTTTTCCCTTCCCCCCCCTTCTTTTCCCCTCTTTTTTCT; CTTCCCCTCTTTCTCCCCCCTTCCTCTCCTTCCCTCCCCTTTCCTCCTTTTCTTTCTCTC; CCCCCCTCCCCTTTCTCTCCCCNCCTTTCCCTTCCCCCNNCCCTCCCTCCNTTTTCTTCT; TCTCCCTCCCCCCTCCCTCCCTCTTCCCTNCTCCCCCGTTTCCCCTCACACTTAAAA;
• Restriction Sites: NotI-NotI
• ACCN: NM_005218
• Insert Size: 530 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_005218.3, NP_005209.1
• RefSeq Size: 484 bp
• RefSeq ORF: 207 bp
• Locus ID: 1672
• UniProt ID: P60022
• Cytogenetics: 8p23.1
• Domains: Defensin_beta
• Protein Families: Secreted Protein
• Summary: Defensins form a family of microbicidal and cytotoxic peptides made by neutrophils. Members of the defensin family are highly similar in protein sequence. This gene encodes defensin, beta 1, an antimicrobial peptide implicated in the resistance of epithelial surfaces to microbial colonization. This gene maps in close proximity to defensin family member, defensin, alpha 1 and has been implicated in the pathogenesis of cystic fibrosis. [provided by RefSeq, Jul 2008]