beta Defensin 1 (DEFB1) (NM_005218) Human Untagged Clone

CAT#: SC116851

DEFB1 (untagged)-Human defensin, beta 1 (DEFB1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: beta Defensin 1
• Synonyms: BD1; DEFB-1; DEFB101; HBD1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_005218 edited
  ATGAGAACTTCCTACCTTCTGCTGTTTACTCTCTGCTTACTTTTGTCTGAGATGGCCTCA
  GGTGGTAACTTTCTCACAGGCCTTGGCCACAGATCTGATCATTACAATTGCGTCAGCAGT
  GGAGGGCAATGTCTCTATTCTGCCTGCCCGATCTTTACCAAAATTCAAGGCACCTGTTAC
  AGAGGGAAGGCCAAGTGCTGCAAGTGA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005218 unedited
  CCGCGAATTCGGCACGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG
  AGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG
  AGAGAGAGAGAGAGAGAGAGCTCAGCCTCCAAAGAAGCCAGCCTCTCCCCAGTTCCTGAA
  ATCCTGGGTGTTGCCTGCCAGTCGCCATGAGAACTTCCTACCTTCTGCTGTTTACTCTCT
  GCTTACTTTTGTCTGAGATGGCCTCAGGTGGTAACTTTCTCACAGGCCTTGGCCACAGAT
  CTGATCATTACAATTGCGTCAGCAGTGGAGGGCAATGTCTCTATTCTGCCTGCCCGATCT
  TTACCAAAATTCAAGGCACCTGTTACAGAGGGAAGGCCAAGTGCTGCAAGTGAGCTGGGA
  GTGACCAGAAGAAATGACGCAGAAGTGAAATGAACTTTTTATAAGCATTCTTTTAATAAA
  GGAAAATTGCTTTTGAAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACTCGACTCTAG
  ATTGCGGCCGCGGTCATAGCTGTTTCCTGAACAGATCCCGGGTGGCATCCCTGTGACCCC
  TCCCCAGTGCCTCTCCTGGGCCCTGGAAGTTGCCACTCCAGTGCCCACCAGCCTTGTCCT
  AATAAATTAAGTTGCATCATTTTGTCTGACTAGGTGTCCTCTATATATATGGGAGGGGGG
  GGGGGNTTNNNNNNNAAACGGGGGGCGTGGTGGAAACG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005218 unedited
  TACCGCAGGCTGNACTTAGATCGAGTAATATATCTTTTTTTTTTTTTTTTTTTTTACTTC
  AAAAGCAATTTTCCTTTATTAAAAGAATGCTTATAAAAAGTTCATTTCACTTCTGCGTCA
  TTTCTTCTGGTCACTCCCAGCTCACTTGCAGCACTTGGCCTTCCCTCTGTAACAGGGGCC
  TTGAATTTTGGTAAAGATCGGGCAGGCAGAATAGAAACATTGCCCTCCACTGCTGACGCA
  ATTGTAATGATCAGATCTGTGGCCAAGGCCTGTGAAAAAGTTACCACCTGAGGCCATCTC
  AGACAAAAGTAAGCAGAGAGTAAACAGCAGAAGGTAGGAAGTTCTCATGGCGACTGGCAG
  GCAACACCCAGGATTTCAGGAACTGGGGAGAGGCTGGCTTCTTTGGAGGCTGAGCTCTCT
  CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTNCCT
  TTCCCCCTCCCCCCTTTCTTTTTTTTTTCTTTTTTTTTCTCCCCCTCCCTCTTTTTNCTC
  TTTCCCTTCCTCCCCCCCTNTTCNCCCTCCCCCCTCCCCCCCCCCTTCTTCCTTCTTCTT
  TCTTTCTTCCTTCCCCCCCNTTTCCTCCCNNCTCCCCTCTCCCTTTTCTCCCCTCCCCCC
  CCCCTCCTTCCCCTTTCCCCTTTCTCCCTCCCCCCTCCTTCTTCTTCCCCCTCCCCCCTC
  CTTTTTTCTCCCCCTCTTTCCCCCCTTCCTTCCCCCCTCCCTTCCCCTCCTTTTCCCTTC
  TTCCCTCCCNCCCCCCCTTCCCTTTTCCCTTCCCCCCCCTTCTTTTCCCCTCTTTTTTCT
  CTTCCCCTCTTTCTCCCCCCTTCCTCTCCTTCCCTCCCCTTTCCTCCTTTTCTTTCTCTC
  CCCCCCTCCCCTTTCTCTCCCCNCCTTTCCCTTCCCCCNNCCCTCCCTCCNTTTTCTTCT
  TCTCCCTCCCCCCTCCCTCCCTCTTCCCTNCTCCCCCGTTTCCCCTCACACTTAAAA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005218
• Insert Size: 530 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005218.3, NP_005209.1
• RefSeq Size: 484 bp
• RefSeq ORF: 207 bp
• Locus ID: 1672
• UniProt ID: P60022
• Cytogenetics: 8p23.1
• Domains: Defensin_beta
• Protein Families: Secreted Protein
• Gene Summary: Defensins form a family of microbicidal and cytotoxic peptides made by neutrophils. Members of the defensin family are highly similar in protein sequence. This gene encodes defensin, beta 1, an antimicrobial peptide implicated in the resistance of epithelial surfaces to microbial colonization. This gene maps in close proximity to defensin family member, defensin, alpha 1 and has been implicated in the pathogenesis of cystic fibrosis. [provided by RefSeq, Jul 2008]