Hyaluronan synthase 2 (HAS2) (NM_005328) Human Untagged Clone

SKU SC116826

HAS2 (untagged)-Human hyaluronan synthase 2 (HAS2)

Product Data

• Type: Human Untagged Clone
• Target Symbol: Hyaluronan synthase 2
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_005328 edited; ATGCATTGTGAGAGGTTTCTATGTATCCTGAGAATAATTGGAACCACACTCTTTGGAGTC; TCTCTCCTCCTTGGAATCACAGCTGCTTATATTGTTGGCTACCAGTTTATCCAAACGGAT; AATTACTATTTCTCTTTTGGACTGTATGGTGCCTTTTTGGCATCACACCTCATCATCCAA; AGCCTGTTTGCCTTTTTGGAGCACCGAAAAATGAAAAAATCCCTAGAAACCCCCATAAAG; TTGAACAAAACAGTTGCCCTTTGCATCGCTGCCTATCAAGAAGATCCAGACTACTTAAGG; AAATGTTTGCAATCTGTGAAAAGGCTAACCTACCCTGGGATTAAAGTTGTCATGGTCATA; GATGGGAACTCAGAAGATGACCTTTACATGATGGACATCTTCAGTGAAGTCATGGGCAGA; GACAAATCAGCCACTTATATCTGGAAGAACAACTTCCACGAAAAGGGTCCCGGTGAGACA; GATGAGTCACATAAAGAAAGCTCGCAACACGTAACGCAATTGGTCTTGTCCAACAAAAGT; ATCTGCATCATGCAAAAATGGGGTGGAAAAAGAGAAGTCATGTACACAGCCTTCAGAGCA; CTGGGACGAAGTGTGGATTATGTACAGGTTTGTGATTCAGACACTATGCTTGACCCAGCC; TCATCTGTGGAGATGGTAAAAGTTTTAGAAGAAGATCCCATGGTTGGAGGTGTTGGGGGA; GATGTCCAGATTTTAAACAAGTACGATTCCTGGATCTCATTCCTCAGCAGTGTAAGATAT; TGGATGGCTTTTAATATAGAAAGGGCCTGTCAGTCTTATTTTGGGTGTGTTCAGTGCATT; AGTGGACCTCTGGGAATGTACAGAAACTCCTTGTTGCATGAGTTTGTGGAAGATTGGTAC; AATCAAGAATTTATGGGCAACCAATGTAGCTTTGGTGATGACAGGCATCTCACGAACCGG; GTGCTGAGCCTGGGCTATGCAACAAAATACACAGCTCGATCTAAGTGCCTTACTGAAACA; CCTATAGAATATCTCAGATGGCTAAACCAGCAGACCCGTTGGAGCAAGTCCTACTTCCGA; GAATGGCTGTACAATGCAATGTGGTTTCACAAACATCACTTGTGGATGACCTACGAAGCG; ATTATCACTGGATTCTTTCCTTTCTTTCTCATTGCCACAGTAATCCAGCTCTTCTACCGG; GGTAAAATTTGGAACATTCTCCTCTTCTTGTTAACTGTCCAGCTAGTAGGTCTCATAAAA; TCATCTTTTGCCAGCTGCCTTAGAGGAAATATCGTCATGGTCTTCATGTCTCTCTACTCA; GTGTTATACATGTCGAGTTTACTTCCCGCCAAGATGTTTGCAATTGCAACAATAAACAAA; GCTGGGTGGGGCACATCAGGAAGGAAAACCATTGTTGTTAATTTCATAGGACTCATTCCA; GTATCAGTTTGGTTTACAATCCTCCTGGGTGGTGTGATTTTCACCATTTATAAGGAGTCT; AAAAGGCCATTTTCAGAATCCAAACAGACAGTTCTAATTGTTGGAACGTTGCTCTATGCA; TGCTATTGGGTCATGCTTTTGACGCTGTATGTAGTTCTCATCAATAAGTGTGGCAGGCGG; AAGAAGGGACAACAATATGACATGGTGCTTGATGTATGA;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005328 unedited; CCATTCCCCCGCCCCGCTGCGCATTGGGCGGTAGGCGTGTCCGGTGGGAGGTCTATATAA; GCAGTTTTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCG; CGAATTCGGCACGAGGAAGAGTTGAACCACCTAAGGCGGAGTTCAAACAAAGGCTGAAGC; AGGAAGGTTTGCTGGGGGAAATCAGTGGGGTCCCCTGAGCAGCGTGAAGTCATCAGGCCA; AGGTCGTCTCAAATTCATCTGATCTCTTATTACCTCAATTTTGGAAACTGCCCGCCACCG; ACCCTCCGGGACCACACAGACAGGCTGAGGACGACTTTATGACCAAGAGCTGAACAAGAT; GCATTGTGAGAGGTTTCTATGTATCCTGAGAATAATTGGAACCACACTCTTTGGAGTCTC; TCTCCTCCTTGGAATCACAGCTGCTTATATTGTTGGCTACCAGTTTATCCAAACGGATAA; TTACTATTTCTCTTTTGGACTGTATGGTGCCTTTTTGGCATCACACCTCATCATCCAAAG; CCTGTTTGCCTTTTTGGAGCACCGAAAAATGAAAAAATCCCTAGAAACCCCCATAAAGTT; GAACAAAACAGTTGCCCTTTGCATCGCTGCCTATCAAGAAGAGCCAGACTACTTAAGGAA; ATGTTTGCAATCTGTGAAAAGGCTAACCTACCCTGNGATTAAAGTTGTCATGGTCATAGA; TGGGAACTCAGCAGATGACCTTTACATGATGGACATCTTCAGTGAAGTCATGGGCAGAGA; CTAATCAGCCACTTATATCTGGAAGACAACTTCCCGAAAAGGGTCCCTGTGAGACAGATG; AGTCACATACAGCAAGCTCGCTACACGTAACGCTAATTGGTCTTGTCCTACACCAGTATC; TGCATCATGCTAAAATGTNNGTGGGACAAGAGCAGTCT;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005328 unedited; CCGGAAATACTATTACGCGGCCGCATTCTANGATCGAGTTTTTTTTTTTTTTTTTTCTTA; TCAAAAATATTTTATTTACAAAAAATAATTATACTATACATCCTATACTGGAAATACATT; GAATAATTGCTAAAATAAATACAGGCAATTAATTCAACTTTTTATAAGAATGAGAGAATT; TGACATTTGAATGTTATCAAAGCTTAACTTAGAACATAATTAGTTAAAAAGGCAAACTCA; AGTTTTCATCTCATTTATTGGCATGGTGTGTTTTGTTTTCCACAAGCTCTCCAATCAGTG; AGAGTCTTGAGTCTCAGATGTACCTTTTTGTACTCTGAAGGTAGGATGTGCAGCTTTTTC; TTAGGCAGGATAGATGTAACATAGATGACTGCCTAAAAAAGAGGCAGAAAGGCAGGAAGC; CCATTTTTTTTTTAACACTTCTCCATGTGTCTCTGCCTCACCATAATGCTGTTTATTCAA; TTATTCATATATTCCGATTGTGTTTTCAACACAGGTTTACACAGCACCAGCTACAAGGCA; AGGTCAATCGTGTAAAAAATAAAACTGGATAATGGAGGCCTTGCAGTAACTAAATTAGCC; AAGTTCAGAGTATGTGGTTCAACAATTTTTATATGTGCCCATCATTGATATACTTATTAA; ACCTTGATGTCACTTCTACTGGGGAGACATTTTTATTCAGGTCCACAACAGGAAATAAGA; AGTGATGATCTGTGTAATTCACAAAAACAAATTCCTCCTTTGTAAAGTAAAAGAGGAAAG; AAACTTGGTGGAGTCTTCTTGCCCAATTTCAAATTATATGGTTTTAAAAATTATCCCACT; TCACCCGGGTAGACCTTTTTACCTCCCTGGTAAAGGGAACG;
• Restriction Sites: NotI-NotI
• ACCN: NM_005328
• Insert Size: 1659 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_005328.1, NP_005319.1
• RefSeq Size: 3003 bp
• RefSeq ORF: 1659 bp
• Locus ID: 3037
• UniProt ID: Q92819
• Cytogenetics: 8q24.13
• Domains: Glycos_transf_2
• Protein Families: Druggable Genome, Transmembrane
• Summary: Hyaluronan or hyaluronic acid (HA) is a high molecular weight unbranched polysaccharide synthesized by a wide variety of organisms from bacteria to mammals, and is a constituent of the extracellular matrix. It consists of alternating glucuronic acid and N-acetylglucosamine residues that are linked by beta-1-3 and beta-1-4 glycosidic bonds. HA is synthesized by membrane-bound synthase at the inner surface of the plasma membrane, and the chains are extruded through pore-like structures into the extracellular space. It serves a variety of functions, including space filling, lubrication of joints, and provision of a matrix through which cells can migrate. HA is actively produced during wound healing and tissue repair to provide a framework for ingrowth of blood vessels and fibroblasts. Changes in the serum concentration of HA are associated with inflammatory and degenerative arthropathies such as rheumatoid arthritis. In addition, the interaction of HA with the leukocyte receptor CD44 is important in tissue-specific homing by leukocytes, and overexpression of HA receptors has been correlated with tumor metastasis. HAS2 is a member of the newly identified vertebrate gene family encoding putative hyaluronan synthases, and its amino acid sequence shows significant homology to glycosaminoglycan synthetase (DG42) from Xenopus laevis, and human and murine hyaluronan synthase 1. [provided by RefSeq, Jul 2008]