TMPRSS2 (NM_005656) Human Untagged Clone

CAT#: SC116619

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TMPRSS2 (untagged)-Human transmembrane protease, serine 2 (TMPRSS2), transcript variant 2



Reconstitution Protocol

SC116619 is the updated version of SC126537.

USD 686.00


Availability*
2 Weeks

Size
    • 10 ug


Product images

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol TMPRSS2
Synonyms PRSS10
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC116619 sequence for NM_005656 edited (data generated by NextGen Sequencing)
ATGGCTTTGAACTCAGGGTCACCACCAGCTATTGGACCTTACTATGAAAACCATGGATAC
CAACCGGAAAACCCCTATCCCGCACAGCCCACTGTGGTCCCCACTGTCTACGAGGTGCAT
CCGGCTCAGTACTACCCGTCCCCCGTGCCCCAGTACGCCCCGAGGGTCCTGACGCAGGCT
TCCAACCCCGTCGTCTGCACGCAGCCCAAATCCCCATCCGGGACAGTGTGCACCTCAAAG
ACTAAGAAAGCACTGTGCATCACCTTGACCCTGGGGACCTTCCTCGTGGGAGCTGCGCTG
GCCGCTGGCCTACTCTGGAAGTTCATGGGCAGCAAGTGCTCCAACTCTGGGATAGAGTGC
GACTCCTCAGGTACCTGCATCAACCCCTCTAACTGGTGTGATGGCGTGTCACACTGCCCC
GGCGGGGAGGACGAGAATCGGTGTGTTCGCCTCTACGGACCAAACTTCATCCTTCAGRTG
TACTCATCTCAGAGGAAGTCCTGGCACCCTGTGTGCCAAGACGACTGGAACGAGAACTAC
GGGCGGGCGGCCTGCAGGGACATGGGCTATAAGAATAATTTTTACTCTAGCCAAGGAATA
GTGGATGACAGCGGATCCACCAGCTTTATGAAACTGAACACAAGTGCCGGCAATGTCGAT
ATCTATAAAAAACTGTACCACAGTGATGCCTGTTCTTCAAAAGCAGTGGTTTCTTTACGC
TGTATAGCCTGCGGGGTCAACTTGAACTCAAGCCGCCAGAGCAGGATTGTGGGCGGYGAG
AGCGCGCTCCCGGGGGCCTGGCCCTGGCAGGTCAGCCTGCACGTCCAGAACGTCCACGTG
TGCGGAGGCTCCATCATCACCCCCGAGTGGATCGTGACAGCCGCCCACTGCGTGGAAAAA
CCTCTTAACAATCCATGGCATTGGACGGCATTTGCGGGGATTTTGAGACAATCTTTCATG
TTCTATGGAGCCGGATACCAAGTAGAAAAAGTGATTTCTCATCCAAATTATGACTCCAAG
ACCAAGAACAATGACATTGCGCTGATGAAGCTGCAGAAGCCTCTGACTTTCAACGACCTA
GTGAAACCAGTGTGTCTGCCCAACCCAGGCATGATGCTGCAGCCAGAACAGCTCTGCTGG
ATTTCCGGGTGGGGGGCCACCGAGGAGAAAGGGAAGACCTCAGAAGTGCTGAACGCTGCC
AAGGTGCTTCTCATTGAGACACAGAGATGCAACAGCAGATATGTCTATGACAACCTGATC
ACACCAGCCATGATCTGTGCCGGCTTCCTGCAGGGGAACGTCGATTCTTGCCAGGGTGAC
AGTGGAGGGCCTCTGGTCACTTCGAAGAACAATATCTGGTGGCTGATAGGGGATACAAGC
TGGGGTTCTGGCTGTGCCAAAGCTTACAGACCAGGAGTGTACGGGAATGTGATGGTATTC
ACGGACTGGATTTATCGACAAATGAGGGCAGACGGCTAA

Clone variation with respect to NM_005656.3
478 g=>r;777 c=>y
>OriGene 5' read for NM_005656 unedited
NCCTGGATCTTGTGTGGGAGTCAGTGTCTACAAGCCTAACTTTTTTCGCGTTGGCTTGGT
TGTTTGGAAAGTCATATTGAACATTCCAGATACCTATCATTACTCGATGCTGTTGATAAC
AGCAAGATGGCTTTGAACTCAGGGTCACCACCAGCTATTGGATCGATTATGAAAACATGG
ATACCATCCGGAAAACCTCTATCCCGCACAGCCCACTGTGGTCCCCACTGTCTACGAGGT
GCATCCGGTTCAGTACTACCCGTCCCCCGTGCCCCAGTACGCCCCGAGGGTCCTGACGCA
GGCTTCCAACCCCGTCGTCTGCACGCAGCCCAAATCCCCATCCGGGACAGTGTGCACCTC
AAAGACTAAGAAAGCACTGTGCATCACCTTGACCCTGGGGACCTTCCTCGTGGGAGCTGC
GCTGGCCGCTGGCCTACTCTGGAAGTTCATGGGCAGCAAGTGCTCCAACTCTGGGATAGA
GTGCGACTCCTCAGGTACCTGCATCAACCCCTCTAACTGGTGTGATGGCGTGTCACACTG
CCCCGGCGGGGAGGACGAGAATCGGTGTGTTCGCCTCTACGGACCAAACTTCATCCTTCA
GGTGTACTCATCTCAGAGGAAGTCCTGGCACCCTGTGTGCCAAGACGACTGGGAACGAGA
ACTACGGGCGGGCGGCCTGCAAGGACATGGGCTATAAGAATAATTTTTACTCTAGCCCAG
GAATATTGGATGACAGCGGATCCACCAGCTTTATGAAACTGAACACAATTGTCCGGCAAT
GTCGATATCTTATAAACACTGTCCCACAATGATGCCTGTTCTTCAAAGT
>OriGene 3' read for NM_005656 unedited
CTCTGGACCGGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTTACTTTGAAA
AAAAAACGCATAATTTATTTGCATGATATTCATTTTCACAATTGAACTTTACAGTTTAAA
AAAGATACAAAAAAAGACAAACAGTTGTTCACATAAATAAGAAGGGGCAATAAAGAAGGA
AGACGTTTTCACCATTACAACACCTTTTAGGATGTGTCTTGGGGAGCAAGCACCTTACAG
TGCCAACTGTTTCCAAGGTCCCTGGGAATGCTGCTCTCTACAGAGGCATGTGCACAGACG
GATCCTGCAAATGGGATTGCAAGACTTTCCATTTCAAGGTTAAGTCCTAGCTGTAGAATC
ATTCATTTCATTCTTGCAAACCAGCCTGCTTGGCCAGGAGGCAGAACCATGGTAGAGTAG
TGCTCATGGTTATGGCACTTGGCAATGCAAAAGGGACCCTTCCCCTGGTTGGAAACCCAC
AGCATTGGAAGGGACCACAGAGAGTCCAAAACAAAACACATCTTTCTCTTCTTCGCCGCC
ACCATGGGCACTTTGCTTCAGCACATTCATTTATAGAACGTTAAGTCAGGAGCCAGTCAT
TTTGTCAAAGCAGCTGAAATAGGCCACCACTCTTACTTGGCAAAGATGATACAGTTTCAA
AGAGTTAAATGAAGGTGGACTACTTGGAGACATCAAAAGCTAAGTTTCCAGGAGCACTGA
GGTAGNCATGTGTAGAAACATAACATGGAAACAGTGTACCTTAAACTGAGCATCCTTGAT
TTNCCCCATGGACCCCTCATTTCAGTGACTAGCAGGCCTGAAGAGGCAACATGGTGCCAG
ACTTNGCGCCTGCCAGGACAGGNGCATGGCACTCTTNCAGGTGCTAGACAGGTCAGGNNA
GATGAGAGAANGCTGATTCTCCTCCAGNAGCCCACCATGTGCAGTGGAAACTGCCCANGA
TGCTAGGCAGTCCTTTCATGCTTCCTCGACAGGACTGT
Restriction Sites ECoRI-NOT     
ACCN NM_005656
Insert Size 3270 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_005656.2, NP_005647.2
RefSeq Size 3226 bp
RefSeq ORF 1479 bp
Locus ID 7113
UniProt ID O15393
Cytogenetics 21q22.3
Domains SR, Tryp_SPc, ldl_recept_a
Protein Families Druggable Genome, Protease, Secreted Protein, Transmembrane
Gene Summary This gene encodes a protein that belongs to the serine protease family. The encoded protein contains a type II transmembrane domain, a receptor class A domain, a scavenger receptor cysteine-rich domain and a protease domain. Serine proteases are known to be involved in many physiological and pathological processes. This gene was demonstrated to be up-regulated by androgenic hormones in prostate cancer cells and down-regulated in androgen-independent prostate cancer tissue. The protease domain of this protein is thought to be cleaved and secreted into cell media after autocleavage. This protein also facilitates entry of viruses into host cells by proteolytically cleaving and activating viral envelope glycoproteins. Viruses found to use this protein for cell entry include Influenza virus and the human coronaviruses HCoV-229E, MERS-CoV, SARS-CoV and SARS-CoV-2 (COVID-19 virus). Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2020]
Transcript Variant: This variant (2) has an alternate 5' exon and uses a downstream AUG start codon, as compared to variant 1. The resulting isoform (2) has a shorter N-terminus, as compared to isoform 1.
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