KIF20A (NM_005733) Human Untagged Clone

SKU SC116593

KIF20A (untagged)-Human kinesin family member 20A (KIF20A)

Product Data

• Type: Human Untagged Clone
• Target Symbol: KIF20A
• Synonyms: MKLP2; RAB6KIFL
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_005733 edited; GAATTCGGCACGGAGGGGAGGAGCAAGTGGCACGTCTTCGGACCTAGGCTGCCCCTGCCG; TCATGTCGCAAGGGATCCTTTCTCCGCCAGCGGGCTTGCTGTCCGATGACGATGTCGTAG; TTTCTCCCATGTTTGAGTCCACAGCTGCAGATTTGGGGTCTGTGGTACGCAAGAACCTGC; TATCAGACTGCTCTGTCGTCTCTACCTCCCTAGAGGACAAGCAGCAGGTTCCATCTGAGG; ACAGTATGGAGAAGGTGAAAGTATACTTGAGGGTTAGGCCCTTGTTACCTTCAGAGTTGG; AACGACAGGAAGATCAGGGTTGTGTCCGTATTGAGAATGTGGAGACCCTTGTTCTACAAG; CACCCAAGGACTCTTTTGCCCTGAAGAGCAATGAACGGGGAATTGGCCAAGCCACACACA; GGTTCACCTTTTCCCAGATCTTTGGGCCAGAAGTGGGACAGGCATCCTTCTTCAACCTAA; CTGTGAAGGAGATGGTAAAGGATGTACTCAAAGGGCAGAACTGGCTCATCTATACATATG; GAGTCACTAACTCAGGGAAAACCCACACGATTCAAGGTACCATCAAGGATGGAGGGATTC; TCCCCCGGTCCCTGGCGCTGATCTTCAATAGCCTCCAAGGCCAACTTCATCCAACACCTG; ATCTGAAGCCCTTGCTCTCCAATGAGGTAATCTGGCTAGACAGCAAGCAGATCCGACAGG; AGGAAATGAAGAAGCTGTCCCTGCTAAATGGAGGCCTCCAAGAGGAGGAGCTGTCCACTT; CCTTGAAGAGGAGTGTCTACATCGAAAGTCGGATAGGTACCAGCACCAGCTTCGACAGTG; GCATTGCTGGGCTCTCTTCTATCAGTCAGTGTACCAGCAGTAGCCAGCTGGATGAAACAA; GTCATCGATGGGCACAGCCAGACACTGCCCCACTACCTGTCCCGGCAAACATTCGCTTCT; CCATCTGGATCTCATTCTTTGAGATCTACAACGAACTGCTTTATGACCTATTAGAACCGC; CTAGCCAACAGCGCAAGAGGCAGACTTTGCGGCTATGCGAGGATCAAAATGGCAATCCCT; ATGTGAAAGATCTCAACTGGATTCATGTGCAAGATGCTGAGGAGGCCTGGAAGCTCCTAA; AAGTGGGTCGTAAGAACCAGAGCTTTGCCAGCACCCACCTCAACCAGAACTCCAGCCGCA; GTCACAGCATCTTCTCAATCAGGATCCTACACCTTCAGGGGGAAGGAGATATAGTCCCCA; AGATCAGCGAGCTGTCACTCTGTGATCTGGCTGGCTCAGAGCGCTGCAAAGATCAGAAGA; GTGGTGAACGGTTGAAGGAAGCAGGAAACATTAACACCTCTCTACACACCCTGGGCCGCT; GTATTGCTGCCCTTCGTCAAAACCAGCAGAACCGGTCAAAGCAGAACCTGGTTCCCTTCC; GTGACAGCAAGTTGACTCGAGTGTTCCAAGGTTTCTTCACAGGCCGAGGCCGTTCCTGCA; TGATTGTCAATGTGAATCCCTGTGCATCTACCTATGATGAAACTCTTCATGTGGCCAAGT; TCTCAGCCATTGCTAGCCAGCTTGTGCATGCCCCACCTATGCAACTGGGATTCCCATCCC; TGCACTCGTTCATCAAGGAACATAGTCTTCAGGTATCCCCCAGCTTAGAGAAAGGGGCTA; AGGCAGACACAGGCCTTGATGATGATATTGAAAATGAAGCTGACATCTCCATGTATGGCA; AAGAGGAGCTCCTACAAGTTGTGGAAGCCATGAAGACACTGCTTTTGAAGGAACGACAGG; AAAAGCTACAGCTGGAGATGCATCTCCGAGATGAAATTTGCAATGAGATGGTAGAACAGA; TGCAACAGCGGGAACAGTGGTGCAGTGAACATTTGGACACCCAAAAGGAACTATTGGAGG; AAATGTATGAAGAAAAACTAAATATCCTCAAGGAGTCACTGACAAGTTTTTACCAAGAAG; AGATTCAGGAGCGGGATGAAAAGATTGAAGAGCTAGAAGCTCTCTTGCAGGAAGCCAGAC; AACAGTCAGTGGCCCATCAGCAATCAGGGTCTGAATTGGCCCTACGGCGGTCACAAAGGT; TGGCAGCTTCTGCCTCCACCCAGCAGCTTCAGGAGGTTAAAGCTAAATTACAGCAGTGCA; AAGCAGAGCTAAACTCTACCACTGAAGAGTTGCATAAGTATCAGAAAATGTTAGAACCAC; CACCCTCAGCCAAGCCCTTCACCATTGATGTGGACAAGAAGTTAGAAGAGGGCCAGAAGA; ATATAAGGCTGTTGCGGACAGAGCTTCAGAAACTTGGTGAGTCTCTCCAATCAGCAGAGA; GAGCTTGTTGCCACAGCACTGGGGCAGGAAAACTTCGTCAAGCCTTGACCACTTGTGATG; ACATCTTAATCAAACAGGACCAGACTCTGGCTGAACTGCAGAACAACATGGTGCTAGTGA; AACTGGACCTTCGGAAGAAGGCAGCATGTATTGCTGAGCAGTATCATACTGTGTTGAAAC; TCCAAGGCCAGGTTTCTGCCAAAAAGCGCCTTGGTACCAACCAGGAAAATCAGCAACCAA; ACCAACAACCACCAGGGAAGAAACCATTCCTTCGAAATTTACTTCCCCGAACACCAACCT; GCCAAAGCTCAACAGACTGCAGCCCTTATGCCCGGATCCTACGCTCACGGCGTTCCCCTT; TACTCAAATCTGGGCCTTTTGGCAAAAAGTACTAAGGCTGTGGGGAAAGAGAAGAGCAGT; CATGGCCCTGAGGTGGGTCAGCTACTCTCCTGAAGAAATAGGTCTCTTTTATGCTTTACC; ATATATCAGGAATTATATCCAGGATGCAATACTCAGACACTAGCTTTTTTCTCACTTTTG; TATTATAACCACCTATGTAATCTCATGTTGTTGTTTTTTTTTATTTACTTATATGATTTC; TATGCACACAAAAACAGTTATATTAAAGATATTATTGTTCACATTTTTTATTGAATTCCA; AATGTAGCAAAATCATTAAAACAAATTATAAAAGGGACAGAAAAATTAAGAATCAAACAT; CATTCTGGACCATGGGAACCTTGAAAAGGCATGGCAGTGGAGACCAGTAACTAGTATACA; GCTTGCCTGAGAAGGCTTAGTAACAAATGAATTCAAGTCGTATTAGATATTCTAAACCTT; CCTTAATATAAGATGAAGTGTCATATGTTAGGAGGTAAAAACGTAACACAAAATTCTGGG; AATAAGAATAAAGTCTGGACCCTGAGCTCAGAATGATCCATAGCATCTACTGTGCAAATA; TATTACAGCAGACATTATAGCATATTACCTCCTGCTGTACACACATGCACAGGCCTGAAA; CTCTCCAAGAGCACCATAAGAATGATTCCCTTTACCTCATCTAGATAAAGGCTGAAAAAA; ATGTATTCTGTTGCAGATGGGAGGGAAAAAAATAGACAATTACGTTGAAAATTTATTAAA; ACAGACCAGCTTGAAAAAAAAAAAAAAAAAACTCGAC;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005733 unedited; GGATTTTGTAATACGACTCACTATTAGGGCGGCCGCGATTCGGCACGGNAGGGAGGAGCA; AGTGGCACGTCTTCGGACCTAGGCTGCCCCTGCCGTCATGTCGCAAGGGATCCTTTCTCC; GCCAGCGGGCTTGCTGTCCGATGACGATGTCGTAGTTTCTCCCATGTTTGAGTCCACAGC; TGCAGATTTGGGGTCTGTGCTACGCAAGAACCTGCTATCAGACTGCTCTGTCGTCTCTAC; CTCCCTAGAGGACAAGCAGCAGGTTCCATCTGAGGACAGTATGGAGAAGGTGAAAGTATA; CTTGAGGGTTAGGCCCTTGTTACCTTCAGAGTTGGAACGACAGGAAGATCAGGGTTGTGT; CCGTATTGAGAATGTGGAGACCCTTGTTCTACAAGCACCCAAGGACTCTTTTGCCCTGAA; GAGCAATGAACGGGGAATTGGCCAAGCCACACACAGGTTCACCTTTTCCCAGATCTTTGG; GCCAGAAGTGGGACAGGCATCCTTCTTCAACCTAACTGTGAAGGAGATGGTAAAGGATGT; ACTCAAAGGGCAGAACTGGCTCATCTATACATATGGAGTCACTAACTCAGGGAAAACCCA; CACGATTCAAGGTACCATCAAGGATGGAGGGATTCTNCCCCGGTCCCTGGCGCTGATCTT; CAATAGCCTCCAGGGCCAACTCATCCAACACCTGATCTGAAGCCCTTGCTCTCAAAATGA; GTATCTGGCTAGACAGCAAGCAGATCCGACAGGAGGAAATGAAGAAGCTGTCCCTGCTAA; TGGAGGCCTCCAAGAGGAGAGCTGTCCACTTCCTGAAGAAGGATGGCTACATCGAAAGTC; GGATAGGTACCACACCAGCTTCGCG;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005733 unedited; CAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTCAAGCTGGTCTGTTTTAATAAATTTTC; AACGTAATTGTCTATTTTTTTCCCTCCCATCTGCAACAGAATACATTTTTTTCAGCCTTT; ATCTAGATGAGGTAAAGGGAATCATTCTTATGGTGCTCTTGGAGAGTTTCAGGCCTGTGC; ATGTGTGTACAGCAGGAGGTAATATGCTATAATGTCTGCTGTAATATATTTGCACAGTAG; ATGCTATGGATCATTCTGAGCTCAGGGTCCAGACTTTATTCTTATTCCCAGAATTTTGTG; TTACGTTTTTACCTCCTAACATATGACACTTCATCTTATATTAAGGAAGGTTTAGAATAT; CTAATACGACTTGAATTCATTTGTTACTAAGCCTTCTCAGGCAAGCTGTATACTAGTTAC; TGGTCTCCACTGCCATGCCTTTTCAAGGTTCCCATGGTCCAGAATGATGTTAGATTCTTA; ATTTTTCTGTCCCTTTTATAATTAGTTTTAATGATTTTGCTACATTTGGAATTCAATAAA; AAATGTGAACAATAATATCTTTAATATAACTGTTTTTGTGTGCATAGAAATCATATANGT; AAATAAAAAAAAACAACAACATGAGATTACATAGGTGGTTATAATACAAAAGTGAGAAAA; AAGCTAGTGTCTGAGTATTGCATCCTGGATATAATTCCTGATATATGGTAAAGCACTAAA; GAGACCTACTTTCTCCAGGAGAGTAGCTGACCCANCTNATGGCCATGACTGCTCTTCTCT; TTCCCACAGCCTTATTACTTNTTGCCAAAGGCCCANATTGAGTAAAGGGAACGCCTGAGC; GTNAGATCCGGCATAGGNCTGCATTCTGTTGAACTTANGCAGNTGTGGTCGGGAATAAAA; TTCACAAGATGGTTCTTCCTGGAGGTGTGGTTAGTTGCGATTTCCTGGTAGAACAAAGCG; CTTTGCAAACCTGCCTTGAGTCCACCAGTTGAACTGCTACATACTGCGCTT;
• Restriction Sites: NotI-NotI
• ACCN: NM_005733
• Insert Size: 3630 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_005733.1, NP_005724.1
• RefSeq Size: 2972 bp
• RefSeq ORF: 2673 bp
• Locus ID: 10112
• UniProt ID: O95235
• Cytogenetics: 5q31.2
• Domains: kinesin
• Protein Families: Druggable Genome
• Summary: Mitotic kinesin required for chromosome passenger complex (CPC)-mediated cytokinesis. Following phosphorylation by PLK1, involved in recruitment of PLK1 to the central spindle. Interacts with guanosine triphosphate (GTP)-bound forms of RAB6A and RAB6B. May act as a motor required for the retrograde RAB6 regulated transport of Golgi membranes and associated vesicles along microtubules. Has a microtubule plus end-directed motility.[UniProtKB/Swiss-Prot Function]