MC4 Receptor (MC4R) (NM_005912) Human Untagged Clone

SKU SC116445

MC4R (untagged)-Human melanocortin 4 receptor (MC4R)

Product Data

• Type: Human Untagged Clone
• Target Symbol: MC4 Receptor
• Synonyms: BMIQ20
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >SC116445 representing NM_005912.; Blue=Insert sequence Red=Cloning site Green=Tag(s); ; ATGGTGAACTCCACCCACCGTGGGATGCACACTTCTCTGCACCTCTGGAACCGCAGCAGTTACAGACTG; CACAGCAATGCCAGTGAGTCCCTTGGAAAAGGCTACTCTGATGGAGGGTGCTACGAGCAACTTTTTGTC; TCTCCTGAGGTGTTTGTGACTCTGGGTGTCATCAGCTTGTTGGAGAATATCTTAGTGATTGTGGCAATA; GCCAAGAACAAGAATCTGCATTCACCCATGTACTTTTTCATCTGCAGCTTGGCTGTGGCTGATATGCTG; GTGAGCGTTTCAAATGGATCAGAAACCATTGTCATCACCCTATTAAACAGTACAGATACGGATGCACAG; AGTTTCACAGTGAATATTGATAATGTCATTGACTCGGTGATCTGTAGCTCCTTGCTTGCATCCATTTGC; AGCCTGCTTTCAATTGCAGTGGACAGGTACTTTACTATCTTCTATGCTCTCCAGTACCATAACATTATG; ACAGTTAAGCGGGTTGGGATCATCATAAGTTGTATCTGGGCAGCTTGCACGGTTTCAGGCATTTTGTTC; ATCATTTACTCAGATAGTAGTGCTGTCATCATCTGCCTCATCACCATGTTCTTCACCATGCTGGCTCTC; ATGGCTTCTCTCTATGTCCACATGTTCCTGATGGCCAGGCTTCACATTAAGAGGATTGCTGTCCTCCCC; GGCACTGGTGCCATCCGCCAAGGTGCCAATATGAAGGGAGCGATTACCTTGACCATCCTGATTGGCGTC; TTTGTTGTCTGCTGGGCCCCATTCTTCCTCCACTTAATATTCTACATCTCTTGTCCTCAGAATCCATAT; TGTGTGTGCTTCATGTCTCACTTTAACTTGTATCTCATACTGATCATGTGTAATTCAATCATCGATCCT; CTGATTTATGCACTCCGGAGTCAAGAACTGAGGAAAACCTTCAAAGAGATCATCTGTTGCTATCCCCTG; GGAGGCCTTTGTGACTTGTCTAGCAGATATTAA;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005912 unedited; ACCTGTAATTGTAAACGACTTACTATAGGCGGCCGCCAGTGTGATGGATATCTGCAGAAT; TCGGCTTATGGTGAACTCCACCCACCGTGGGATGCACACTTCTCTGCACCTCTGGAACCG; CAGCAGTTACAGACTGCACAGCAATGCCAGTGAGTCCCTTGGAAAAGGCTACTCTGATGG; AGGGTGCTACGAGCAACTTTTTGTCTCTCCTGAGGTGTTTGTGACTCTGGGTGTCATCAG; CTTGTTGGAGAATATCTTAGTGATTGTGGCAATAGCCAAGAACAAGAATCTGCATTCACC; CATGTACTTTTTCATCTGCAGCTTGGCTGTGGCTGATATGCTGGTGAGCGTTTCAAATGG; ATCAGAAACCATTGTCATCACCCTATTAAACAGTACAGATACGGATGCACAGAGTTTCAC; AGTGAATATTGATAATGTCATTGACTCGGTGATCTGTAGCTCCTTGCTTGCATCCATTTG; CAGCCTGCTTTCAATTGCAGTGGACAGGTACTTTACTATCTTCTATGCTCTCCAGTACCA; TAACATTATGACAGTTAAGCGGGTTGGGATCATCATAAGTTGTATCTGGGCAGCTTGCAC; GGTTTCAGGCATTNTGTTCATCATTTACTCAGATAGTAGTGCTGTCATCATCTGCCTCAT; CACCATGTTCTTCACCATGCTGGCTCTCATGGCTTCTCTCTATGTCCACATGTTCCTGAT; GGCCAGGCTTCACATTAAGAGGATTGCTGTCCTCCCCGCACTGGTGCCATCCGCCAGGTG; CCAATATGAAGGGAGCGATTACCTTGACCATCCTGATTGGCGTCTTTTGTGTCTGCTGGG; CCCCATTCTTNCTNCACTGATATTCTACATCTCTTGTCCTCAGAATCATATTGNGTGTGC; TTCATGTCTCACTTTAACTGNATCTATACTGANCATGTGTAAATCATCATC;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005912 unedited; CGGCCGCTTTCTANGATCGACAAACTTGGAATCGGTACCGAGCTCGGATCCACTAGTAAC; GGCCGCCAGTGTGCTGGAATTCGGCTTTTAATATCTGCTAGACAAGTCACAAAGGCCTCC; CAGGGGATAGCAACAGATGATCTCTTTGAAGGTTTTCCTCAGTTCTTGACTCCGGAGTGC; ATAAATCAGAGGATCGATGATTGAATTACACATGATCAGTATGAGATACAAGTTAAAGTG; AGACATGAAGCACACACAATAT;
• Restriction Sites: SgfI-MluI
• ACCN: NM_005912
• Insert Size: 999 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_005912.1
• RefSeq Size: 1438 bp
• RefSeq ORF: 999 bp
• Locus ID: 4160
• UniProt ID: P32245
• Cytogenetics: 18q21.32
• Protein Families: Druggable Genome, GPCR, Transmembrane
• Protein Pathways: Neuroactive ligand-receptor interaction
• MW: 36.9 kDa
• Summary: The protein encoded by this gene is a membrane-bound receptor and member of the melanocortin receptor family. The encoded protein interacts with adrenocorticotropic and MSH hormones and is mediated by G proteins. This is an intronless gene. Defects in this gene are a cause of autosomal dominant obesity. [provided by RefSeq, Jan 2010]