AKR1D1 (NM_005989) Human Untagged Clone

SKU SC116410

AKR1D1 (untagged)-Human aldo-keto reductase family 1, member D1 (delta 4-3-ketosteroid-5-beta-reductase) (AKR1D1), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: AKR1D1
• Synonyms: 3o5bred; CBAS2; SRD5B1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_005989 edited; ATGGATCTCAGTGCTGCAAGTCACCGCATACCTCTAAGTGATGGAAACAGCATTCCCATC; ATCGGACTTGGTACCTACTCAGAACCTAAATCGACCCCTAAGGGAGCCTGTGCAACATCG; GTGAAGGTTGCTATTGACACAGGGTACCGACATATTGATGGGGCCTACATCTACCAAAAT; GAACACGAAGTTGGGGAGGCCATCAGGGAGAAGATAGCAGAAGGAAAGGTGCGGAGGGAA; GATATCTTCTACTGTGGAAAGCTATGGGCTACAAATCATGTCCCAGAGATGGTCCGCCCA; ACCCTGGAGAGGACACTCAGGGTCCTCCAGCTAGATTATGTGGATCTTTACATCATTGAA; GTACCCATGGCCTTTAAGCCAGGAGATGAAATATACCCTAGAGATGAGAATGGCAAATGG; TTATATCACAAGTCAAATCTGTGTGCCACTTGGGAGGCGATGGAAGCTTGCAAAGACGCT; GGCTTGGTGAAATCCCTGGGAGTGTCCAATTTTAACCGCAGGCAGCTGGAGCTCATCCTG; AACAAGCCAGGACTCAAACACAAGCCAGTCAGCAACCAGGTTGAGTGCCATCCGTATTTC; ACCCAGCCAAAACTCTTGAAATTTTGCCAACAACATGACATTGTCATTACTGCATATAGC; CCTTTGGGGACCAGTAGGAATCCAATCTGGGTGAATGTTTCTTCTCCACCTTTGTTAAAG; GATGCACTTCTAAACTCATTGGGGAAAAGGTACAATAAGACAGCAGCTCAAATTGTTTTG; CGTTTCAACATCCAGCGAGGGGTGGTTGTCATTCCTAAAAGCTTTAATCTTGAAAGGATC; AAAGAAAATTTTCAGATCTTTGACTTTTCTCTCACTGAAGAAGAAATGAAGGACATTGAA; GCCTTGAATAAAAATGTCCGCTTTGTAGAATTGCTCATGTGGCGCGATCATCCTGAATAC; CCATTTCATGATGAATACTGA;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005989 unedited; NNGGTCAGAATTTGTATACGACTCATATAGGGCGGCCGCGAATTCGCACCAGCACAATGG; ATCTCAGTGCTGCAAGTCACCGCATACCTCTAAGTGATGGAAACAGCATTCCCATCATCG; GACTTGGTACCTACTCAGAACCTAAATCGACCCCTAAGGGAGCCTGTGCAACATCGGTGA; AGGTTGCTATTGACACAGGGTACCGACATATTGATGGGGCCTACATCTACCAAAATGAAC; ACGAAGTTGGGGAGGCCATCAGGGAGAAGATAGCAGAAGGAAAGGTGCGGAGGGAAGATA; TCTTCTACTGTGGAAAGCTATGGGCTACAAATCATGTCCCAGAGATGGTCCGCCCAACCC; TGGAGAGGACACTCAGGGTCCTCCAGCTAGATTATGTGGATCTTTACATCATTGAAGTAC; CCATGGCCTTTAAGCCAGGAGATGAAATATACCCTAGAGATGAGAATGGCAAATGGTTAT; ATCACAAGTCAAATCTGTGTGCCACTTGGGAGGCGATGGAAGCTTGCAAAGACGCTGGCT; TGGTGAAATCCCTGGGAGTGTCCAATTTTAACCGCAGGCAGCTGGAGCTCATCCTGAACA; AGCCAGGACTCAAACACAAGCCAGTCAGCAACCAGGTTGAGTGCCATCCGTATTTCACCC; AGCCAAAACTCTTGAAATTTTGCCAACAACATGACATTGTCATTACTGCATATAGCCCTT; TGGGGACCAGTANGAATCCAATCTGGGTGAATGTTTCTTCTCCACCTTTGTTAAAGGATG; CACTTCTAAACTCATTGGGGAAAAGGTACAATAAGACAGCAGCTCANATTGTTTTTGCGT; TTCAACATCCAGCGAGGGGGTGGTTGTCATTCCTAAAAGCTTTAATCTTGAAAGGATCAA; AGAAAATTTTCAGATCTTTGACTTTTT;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005989 unedited; TTGACGCATTGGCGATTGGCACCTTTCCAGGTCCAGTACAGTCACTGGGGAATGGGTCAC; AGGGCTGCCCCCCGTGTTCTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTATATTC; GAGTTTTTTTTTTTTTTTTTTTTTTTTTGATGCAACAAATCATTATACTTTATTATTTCT; TGTGAACAAAATATTTTCCAGAGGTCAGTGTTATAAGTAATAATATTGGAATCAGTGAAA; ACAATCAGTATAGACCAAATTTTTTAAAATGATAATTTCTCAAAAATATATGTCTTGAGG; TCATTGAACATTATCTATAAGCAACTTATCTTGGGAAAACATAATTTTTGAAGAACTCTC; TCATCCTATGACAGTATGACAGTGACCTAATGTGAATGAATAAAATATATTTCTTTTCTT; TTTTTTTTTGTTTTTGAGACAGAGTCTCGCTCTTTCACCCAGGCCGGACTGCAGTGGCGC; TATCTCGGCTCACTGCAAGCTCCTCCTCCCGGGTTCATGCCATTCTTCTGCCTCAACCTC; CTGAGTAGCTGGGATTACAGGCCCCCGCCACCGCGCCTGGCTATTTTTTTTTAAAAAAAA; AAAAAAACCAGNNTTNACACCTTTTTAGCCCAGAATGTCTTCGTTCTCCTGACCTCCTGA; TCTGCCCACCTTGGCCTCCCCAAGTGCTTGTATTTTTGGCATGAGCCCCCCGCCCCCGGC; CAAATAAATATTTTTCTGTAGTTGAATTGAAAATAGATTATTCTCTTTTGCTGAATTTCA; ATAATCTTATTCACTCTTTAAATTGTCCCTCCCGGAAAATTTCCAAATTTCTTTTTAACT; TGCTTTCTCAAAATTCATTTAAAATTTCCAATTTCTTTTAGCTTTTCCAATTACACTTTT; AAATTCTTAAGTCCATTATTCTTAAAAT;
• Restriction Sites: NotI-NotI
• ACCN: NM_005989
• Insert Size: 981 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_005989.2, NP_005980.1
• RefSeq Size: 2692 bp
• RefSeq ORF: 981 bp
• Locus ID: 6718
• UniProt ID: P51857
• Cytogenetics: 7q33
• Domains: aldo_ket_red
• Protein Families: Druggable Genome
• Protein Pathways: Androgen and estrogen metabolism, C21-Steroid hormone metabolism, Metabolic pathways, Primary bile acid biosynthesis
• Summary: The enzyme encoded by this gene is responsible for the catalysis of the 5-beta-reduction of bile acid intermediates and steroid hormones carrying a delta(4)-3-one structure. Deficiency of this enzyme may contribute to hepatic dysfunction. Three transcript variants encoding different isoforms have been found for this gene. Other variants may be present, but their full-length natures have not been determined yet. [provided by RefSeq, Jul 2010]; Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.