Melatonin Receptor 1A (MTNR1A) (NM_005958) Human Untagged Clone

SKU SC116399

MTNR1A (untagged)-Human melatonin receptor 1A (MTNR1A)

Product Data

• Type: Human Untagged Clone
• Target Symbol: Melatonin Receptor 1A
• Synonyms: MEL-1A-R; MT1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_005958, RT-PCR generated; ATGCAGGGCAACGGCAGCGCGCTGCCCAACGCCTCCCAGCCCGTGCTCCGCGGGGACGGC; GCGCGGCCCTCGTGGCTGGCGTCCGCCCTGGCCTGCGTCCTCATCTTCACCATCGTGGTG; GACATCCTGGGCAACCTCCTGGTCATCCTGTCGGTGTATCGGAACAAGAAGCTCAGGAAC; GCAGGAAACATCTTTGTGGTGAGCTTAGCGGTGGCAGACCTGGTGGTGGCCATTTATCCG; TACCCGTTGGTGCTGATGTCGATATTTAACAACGGGTGGAACCTGGGCTATCTGCACTGC; CAAGTCAGTGGGTTCCTGATGGGCCTGAGCGTCATCGGCTCCATATTCAACATCACCGGC; ATCGCCATCAACCGCTACTGCTACATCTGCCACAGTCTCAAGTACGACAAACTGTACAGC; AGCAAGAACTCCCTCTGCTACGTGCTCCTCATATGGCTCCTGACGCTGGCGGCCGTCCTG; CCCAACCTCCGTGCAGGGACTCTCCAGTACGACCCGAGGATCTACTCGTGCACCTTCGCC; CAGTCCGTCAGCTCCGCCTACACCATCGCCGTGGTGGTTTTCCACTTCCTCGTCCCCATG; ATCATAGTCATCTTCTGTTACCTGAGAATATGGATCCTGGTTCTCCAGGTCAGACAGAGG; GTGAAACCTGACCGCAAACCCAAACTGAAACCACAGGACTTCAGGAATTTTGTCACCATG; TTTGTGGTTTTTGTCCTTTTTGCCATTTGCTGGGCTCCTCTGAACTTCATTGGCCTGGCC; GTGGCCTCTGACCCCGCCAGCATGGTGCCTAGGATCCCAGAGTGGCTGTTTGTGGCCAGT; TACTACATGGCGTATTTCAACAGCTGCTTCAATGCCATTATATACGGGCTACTGAACCAA; AATTTCAGGAAGGAATACAGGAGAATTATAGTCTCGCTCTGTACAGCCAGGGTGTTCTTT; GTGGACAGCTCTAACGACGTGGCCGATAGGGTTAAATGGAAACCGTCTCCACTGATGACC; AACAATAATGTAGTAAAGGTGGACTCCGTTTAA;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005958 unedited; GGCCGAGGTCAAGATTTTGTATACGACTCACTATAGGGCGGCCGCCAGTGTGATGGAATC; TGCAGAATTCGGCTTGACGCGAACAGGGACCATGCAGGGCAACGGCAGCGCGCTGCCCAA; CGCCTCCCAGCCCGTGCTCCGCGGGGACGGCGCGCGGCCCTCGTGGCTGGCGTCCGCCCT; GGCCTGCGTCCTCATCTTCACCATCGTGGTGGACATCCTGGGCAACCTCCTGGTCATCCT; GTCGGTGTATCGGAACAAGAAGCTCAGGAACGCAGGAAACATCTTTGTGGTGAGCTTAGC; GGTGGCAGACCTGGTGGTGGCCATTTATCCGTACCCGTTGGTGCTGATGTCGATATTTAA; CAACGGGTGGAACCTGGGCTATCTGCACTGCCAAGTCAGTGGGTTCCTGATGGGCCTGAG; CGTCATCGGCTCCATATTCAACATCACCGGCATCGCCATCAACCGCTACTGCTACATCTG; CCACAGTCTCAAGTACGACAAACTGTACAGCAGCAAGAACTCCCTCTGCTACGTGCTCCT; CATATGGCTCCTGACGCTGGCGGCCGTCCTGCCCAACCTCCGTGCAGGGACTCTCCAGTA; CGACCCGAGGATCTACTCGTGCACCTTCGCCCAGTCCGTCAGCTCCGCCTACACCATCGC; CGTGGGTGGTTTTCCACTTCCTCGTCCCCATGATCATAGTCATCTTCTGTTACCTGAGAA; TATGGATCCTGGTTCTCCAGGTCAGACAGAGGGTGAAACCTGACCGCANACCCAAACTGA; NACCACAGGACTTCAGGAATTTTGTCCCATGTTTGTGGGGTTTTGTCCTTTTTGCCATTT; GCTGGGCTCCTCTGAACTCATTGGCCTGGCCGTGGCCTCTGACCCCGCAGCATGTGCCCT; AGATCCCAGATGGCN;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005958 unedited; GGGGACTAGGAATGGAAACTTCCATGCCAGNAAAGCACTGGGGNAGGGTCACAGGGCTGC; CACCCGGGATCTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGACAAGCT; TGATATCGGTACCGAGCTCGGATCCACTAGTAACGGCCGCCAGTGTGCTGGAATTCGGCT; TTTAAACGGAGTCCACCTTTACTACATTATTGTTGGTCATCAGTGGAGACGGTTTCCATT; TAACCCTATCGGCCACGTCGTTAGAGCTGTCCACAAAGAACACCCTGGCTGTACAGAGCG; AGACTATAATTCTCCTGTATTCCTTCCTGAAATTTTGGTTCAGTAGCCCGTATATAATGG; CATTGAAGCAGCTGTTGAAATACGCCATGTAGTAACTGGCCACAAACAGCCACTCTGGGA; TCCTAGGCACCATGCTGGCGGGGTCAGAGGCCACGGCCAGGCCAATGAAGTTCAGAGGAG; CCCAGCAAATGGCAAAAAGGACAAAAACCACAAACATGGTGACCAAATTCCTGAAGTCCT; GTGGTTTCAGTTTGGGTTTGCGGTCAGGTTTCACCCTCTGTCTGACCTGGAGAACCAGGA; TCCATATTCTCAGGTAACAAGAGATGACTATGATCATGGGGACGAGGAAGTGGAAAACCA; CCCCGCGATGGTGTAAGCGGACCTGACNGACTGGGCGAAAGTGCACAAATAAATCCTCGG; GTCCGACTGGGAGAGTCCCTGCACGGGAGGTTGGGCCAGGACGGCCCGCCACCGTCAGGA; GCCCTTATGAAGAGCACGTACCAAAAGGAGTTCCTTGCTGCTGGCAAGTTTTGGTCGACT; TGAAAATGGGGCAAAGTATAAATAGCGGG;
• Restriction Sites: Please inquire
• ACCN: NM_005958
• Insert Size: 1300 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_005958.3, NP_005949.1
• RefSeq Size: 1105 bp
• RefSeq ORF: 1053 bp
• Locus ID: 4543
• UniProt ID: P48039
• Cytogenetics: 4q35.2
• Protein Families: Druggable Genome, GPCR, Transmembrane
• Protein Pathways: Neuroactive ligand-receptor interaction
• Summary: This gene encodes one of two high affinity forms of a receptor for melatonin, the primary hormone secreted by the pineal gland. This receptor is a G-protein coupled, 7-transmembrane receptor that is responsible for melatonin effects on mammalian circadian rhythm and reproductive alterations affected by day length. The receptor is an integral membrane protein that is readily detectable and localized to two specific regions of the brain. The hypothalamic suprachiasmatic nucleus appears to be involved in circadian rhythm while the hypophysial pars tuberalis may be responsible for the reproductive effects of melatonin. [provided by RefSeq, Jul 2008]