PECI (ECI2) (NM_006117) Human Untagged Clone

SKU SC116338

ECI2 (untagged)-Human enoyl-CoA delta isomerase 2 (ECI2), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: PECI
• Synonyms: ACBD2; dJ1013A10.3; DRS-1; DRS1; HCA88; PECI
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC116338 sequence for NM_006117 edited (data generated by NextGen Sequencing); ATGAATAGAACAGCAATGAGAGCCAGTCAGAAGGACTTTGAAAATTCAATGAATCAAGTG; AAACTCTTGAAAAAGGATCCAGGAAACGAAGTGAAGCTAAAACTCTACGCGCTATATAAG; CAGGCCACTGAAGGACCTTGTAACATGCCCAAACCAGGTGTATTTGACTTGATCAACAAG; GCCAAATGGGACGCATGGAATGCCCTTGGCAGCCTGCCCAAGGAAGCTGCCAGGCAGAAC; TATGTGGATTTGGTGTCCAGTTTGAGTCCTTCATTGGAATCCTCTAGTCAGGTGGAGCCT; GGAACAGACAGGAAATCAACTGGGTTTGAAACTCTGGTGGTGACCTCCGAAGATGGCATC; ACAAAGATCATGTTCAACCGGCCCAAAAAGAAAAATGCCATAAACACTGAGATGTATCAT; GAAATTATGCGTGCACTTAAAGCTGCCAGCAAGGATGACTCAATCATCACTGTTTTAACA; GGAAATGGTGACTATTACAGTAGTGGGAATGATCTGACTAACTTCACTGATATTCCCCCT; GGTGGAGTAGAGGAGAAAGCTAAAAATAATGCCGTTTTACTGAGGGAATTTGTGGGCTGT; TTTATAGATTTTCCTAAGCCTCTGATTGCAGTGGTCAATGGTCCAGCTGTGGGCATCTCC; GTCACCCTCCTTGGGCTATTCGATGCCGTGTATGCATCTGACAGGGCAACATTTCATACA; CCATTTAGTCACCTAGGCCAAAGTCCGGAAGGATGCTCCTCTTACACTTTTCCGAAGATA; ATGAGCCCAGCCAAGGCAACAGAGATGCTTATTTTTGGAAAGAAGTTAACAGCGGGAGAG; GCATGTGCTCAAGGACTTGTTACTGAAGTTTTCCCTGATAGCACTTTTCAGAAAGAAGTC; TGGACCAGGCTGAAGGCATTTGCAAAGCTTCCCCCAAATGCCTTGAGAATTTCAAAAGAG; GTAATCAGGAAAAGAGAGAGAGAAAAACTACACGCTGTTAATGCTGAAGAATGCAATGTC; CTTCAGGGAAGATGGCTATCAGATGAATGCACAAATGCTGTGGTGAACTTCTTATCCAGA; AAATCAAAACTGTGA; ; Clone variation with respect to NM_006117.2;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006117 unedited; GATTTTGTAATACGACTCACTTATAGGGCGGCCGCGAATTCGCACGAGGGGATGGCGATG; GCGTACTTGGCTTGGAGACTGGCGCGGCGTTCGTGTCCGAGTTCTCTGCAGGTCACTAGT; TTCCCGGTAGTTCAGCTGCACATGAATAGAACAGCAATGAGAGCCAGTCAGAAGGACTTT; GAAAATTCAATGAATCAAGTGAAACTCTTGAAAAAGGATCCAGGAAACGAAGTGAAGCTA; AAACTCTACGCGCTATATAAGCAGGCCACTGAAGGACCTTGTAACATGCCCAAACCAGGT; GTATTTGACTTGATCAACAAGGCCAAATGGGACGCATGGAATGCCCTTGGCAGCCTGCCC; AAGGAAGCTGCCAGGCAGAACTATGTGGATTTGGTGTCCAGTTTGAGTCCTTCATTGGAA; TCCTCTAGTCAGGTGGAGCCTGGAACAGACAGGAAATCAACTGGGTTTGAAACTCTGGTG; GTGACCTCCGAAGATGGCATCACAAAGATCATGTTCAACCGGCCCAAAAGAAAAATGCCA; TAAACACTGAGATGTATCATGAAATTATGCGTGCCTTAAAGCTGCCGCAAGGATGACTCA; TCATCACTGTTTTACAGGAAATGGTGACTATTACAGTAGTGGGAATGATCTGACTAACTT; ACTGATATTCCCCCTGGTGGAGTAGAGGAGAAAGCANAATNATGCCGTTTTACTGAGGAA; TTGTGGGGCTGTTTATAGATTTCCTAGCCTCTGATTGCAGTGGTCATGGTCCAGCTGTGG; CATCTCCGTCACCTTCTTGGGCTATCCGATGGCGTGTATGCATCTGACAGGGGCACATTC; ATACACCATTTATCACTAGGGCCAGGNCCGGGAGGGAGCTCCTCTTACACTTTTCGAGAT; ATGGAGCCAGCC;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_006117 unedited; AATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGTTTTACAAAACTTTTTATTCATCAGAG; CTGTAGTGAAATATCATCATTGTAATTGATATTCTAGCACTACAAAAGGCACAATGAAGC; TTATTTAGTTCCAGTACTGGAAATCAGAGGTAACAGCACATCCTTCCTTGGACATGCTTT; ACTCTGCTGTAGTGGTCATCACAGTTTTGATTTTCTGGATAAGAAGTTCACCACAGCATT; TGTGCATTCATCTGATAGCCATCTTCCCTGAAGGACATTGCATTCTTCAGCATTAACAGC; GTGTAGTTTTTCTCTCTCTCTTTTCCTGATTACCTCTTTTGAAATTCTCAAGGCATTTGG; GGGAAGCTTTGCAAATGCCTTCAGCCTGGTCCAGACTTCTTTCTGAAAAGTGCTATCAGG; GAAAACTTCAGTAACAAGTCCTTGAGCACATGCCTCTCCCGCTGTTAACTTCTTTCCAAA; AATAAGCATCTCTGTTGCCTTGGCTGGGCTCATTATCTTCGGAAAAGTGTAAGAGGAGCA; TCCTTCCGGACTTTGGCCTAGGTGACTAAATGGTGTATGAAATGTTGCCCTGTCAGATGC; ATACACGGCATCGAATAGCCCAAGAGGGTGACGGAGATGCCCACAGCTGGACCATTGACC; ACTGCATCAGAGGCTTANGAAAATCTATANACAGCCACANATTCCCTCAGTAAACGCATT; ATTNNTAGCTTCTNCTCTACTCACCAGGGGGATATCANTGAAGTAGTCAGATCATCCCAC; TCTGTATAGTCACCATTTCTGGTAAACATGATGATGAGTCATCTGCTGGAGCTTAAGTGC; ACGCTATTATGATCATTTANNGGTATGCATTTTCTTTTGGGCGTGAAATGAACTTGATGC; TTTTGAGGACCCCAATTCAAACAATGATTTCGTTGCCAGCTCCTGGTTAGATCAAGAGCT; AACGA;
• Restriction Sites: NotI-NotI
• ACCN: NM_006117
• Insert Size: 1460 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_006117.2, NP_006108.2
• RefSeq Size: 1410 bp
• RefSeq ORF: 1095 bp
• Locus ID: 10455
• UniProt ID: O75521
• Cytogenetics: 6p25.2
• Domains: ACBP, ECH
• Protein Pathways: Fatty acid metabolism
• Summary: This gene encodes a member of the hydratase/isomerase superfamily. The protein encoded is a key mitochondrial enzyme involved in beta-oxidation of unsaturated fatty acids. It catalyzes the transformation of 3-cis and 3-trans-enoyl-CoA esters arising during the stepwise degradation of cis-, mono-, and polyunsaturated fatty acids to the 2-trans-enoyl-CoA intermediates. Alternatively spliced transcript variants have been described. [provided by RefSeq, Aug 2011]; Transcript Variant: This variant (1) encodes the shorter isoform (1). Both variants 1 and 3 encode the same isoform.