beta III Tubulin (TUBB3) (NM_006086) Human Untagged Clone

SKU SC116313

TUBB3 (untagged)-Human tubulin, beta 3 (TUBB3), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: beta III Tubulin
• Synonyms: beta-4; CDCBM; CDCBM1; CFEOM3; CFEOM3A; FEOM3; TUBB4
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC116313 sequence for NM_006086 edited (data generated by NextGen Sequencing); ATGAGGGAGATCGTGCACATCCAGGCCGGCCAGTGCGGCAACCAGATCGGGGCCAAGTTC; TGGGAAGTCATCAGTGATGAGCATGGCATCGACCCCAGCGGCAACTACGTGGGCGACTCG; GACTTGCAGCTGGAGCGGATCAGCGTCTACTACAACGAGGCCTCTTCTCACAAGTACGTG; CCTCGAGCCATTCTGGTGGACCTGGAACCCGGAACCATGGACAGTGTCCGCTCAGGGGCC; TTTGGACATCTCTTCAGGCCTGACAATTTCATCTTTGGTCAGAGTGGGGCCGGCAACAAC; TGGGCCAAGGGTCACTACACGGAGGGGGCGGAGCTGGTGGATTCGGTCCTGGATGTGGTG; CGGAAGGAGTGTGAAAACTGCGACTGCCTGCAGGGCTTCCAGCTGACCCACTCGCTGGGG; GGCGGCACGGGCTCCGGCATGGGCACGTTGCTCATCAGCAAGGTGCGTGAGGAGTATCCC; GACCGCATCATGAACACCTTCAGCGTCGTGCCCTCACCCAAGGTGTCAGACACGGTGGTG; GAGCCCTACAACGCCACGCTGTCCATCCACCAGCTGGTGGAGAACACGGATGAGACCTAC; TGCATCGACAACGAGGCGCTCTACGACATCTGCTTCCGCACCCTCAAGCTGGCCACGCCC; ACCTACGGGGACCTCAACCACCTGGTATCGGCCACCATGAGCGGAGTCACCACCTCCTTG; CGCTTCCCGGGCCAGCTCAACGCTGACCTGCGCAAGCTGGCCGTCAACATGGTGCCCTTC; CCGCGCCTGCACTTCTTCATGCCCGGCTTCGCCCCCCTCACAGCCCGGGGCAGCCAGCAG; TACCGGGCCCTGACCGTGCCCGAGCTCACCCAGCAGATGTTCGATGCCAAGAACATGATG; GCCGCCTGCGACCCGCGCCACGGCCGCTACCTGACGGTGGCCACCGTGTTCCGGGGCCGC; ATGTCCATGAAGGAGGTGGACGAGCAGATGCTGGCCATCCAGAGCAAGAACAGCAGCTAC; TTCGTGGAGTGGATCCCCAACAACGTGAAGGTGGCCGTGTGTGACATCCCGCCCCGCGGC; CTCAAGATGTCCTCCACCTTCATCGGGAACAGCACGGCCATCCAGGAGCTGTTCAAGCGC; ATCTCCGAGCAGTTCACGGCCATGTTCCGGCGCAAGGCCTTCCTGCACTGGTACACGGGC; GAGGGCATGGACGAGATGGAGTTCACCGAGGCCGAGAGCAACATGAACGACCTGGTGTCC; GAGTACCAGCAGTACCAGGACGCCACGGCCGAGGAAGAGGGCGAGATGTACGAAGACGAC; GAGGAGGAGTCGGAGGCCCAGGGCCCCAAGTGA; ; Clone variation with respect to NM_006086.3;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006086 unedited; TCAACATTTGTATACGACTCATATAGGCGGCNCGCGAATTCGCACGAGGCCAGTATGAGG; GAGATCGTGCACATCCAGGCCGGCCAGTGCGGCAACCAGATCGGGGCCAAGTTCTGGGAA; GTCATCAGTGATGAGCATGGCATCGACCCCAGCGGCAACTACGTGGGCGACTCGGACTTG; CAGCTGGAGCGGATCAGCGTCTACTACAACGAGGCCTCTTCTCACAAGTACGTGCCTCGA; GCCATTCTGGTGGACCTGGAACCCGGAACCATGGACAGTGTCCGCTCAGGGGCCTTTGGA; CATCTCTTCAGGCCTGACAATTTCATCTTTGGTCAGAGTGGGGCCGGCAACAACTGGGCC; AAGGGTCACTACACGGAGGGGGCGGAGCTGGTGGATTCGGTCCTGGATGTGGTGCGGAAG; GAGTGTGAAAACTGCGACTGCCTGCAGGGCTTCCAGCTGACCCACTCGCTGGGGGGCGGC; ACGGGCTCCGGCATGGGCACGTTGCTCATCAGCAAGGTGCGTGAGGAGTATCCCGACCGC; ATCATGAACACCTTCAGCGTCGTGCCCTCACCCAAGGTGTCAGACACGGTGGTGGAGCCC; TACAACGCCACGCTGTCCATCCACCAGCTGGTGGAGAACACGGATGAGACCTACTGCATC; GACAACGAGGCGCTCTACGACATCTGCTTCCGCACCCTCAAGCTGGCCACGCCCACCTAC; GGNNGACCTCACCACCTGGTATCGGCCACCATGAGCGGAGTCACCACCTCCTTGCGCTTC; CNGGGCCAGCTCAACGCTGACCTGCGNCAGCTGGGCCGTCACATGGTGCCCTTCCGCGCC; TGCACTTCTTATGCCCCGCTTCGCCCCCTCACAGCCCGGGCAGCCAGCAGTCCGGGCCCT; GACGTGCCCGAGCTCAC;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_006086 unedited; GCCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTATCTGACAGCAATAGA; TTTATTAAGTATCCCCGAAAATTAAACACAAACCAGTAAAAAACAAAACCGTAAAACGTC; AGGCCTGGAGCTGCAATAAGACAGAGACAGGAGCAGCTCACACGTGGCCTAGGTGGGGAG; GACGAGGCCATAAATACTGCAGGAGGGCGGCAAGGGAGCCCTAGGGCGAGGGGAAAGCAG; GGTGTCGGCAGCAAGATGGCTCCGGGGGTTTAGACACTGCTGGCTTCGGCCCCGGCCGCC; ACCTGCCTCTCACTCCAGCTGCGAGCAGCTTCACTTGGGGCCCTGGGCCTCCGACTCCTC; CTCGTCGTCTTCGTACATCTCGCCCTCTTCCTCGGCCGTGGCGTCCTGGTACTGCTGGTA; CTCGGACACCAGGTCGTTCATGTTGCTCTCGGCCTCGGTGAACTCCATCTCGTCCATGCC; CTCGCCCGTGTACCAGTGCAGGAAGGCCTTGCGCCGGAACATGGCCGTGAACTGCTCGGA; GATGCGCTTGAACAGCTCCTGGATGGCCGTGCTGTTCCCGATGAAGGTGGAGGACATCTT; GAGGCCGCGGNGCGGGATGTCACACACGGCCACCTTCACGTTGTTGGGGGATCCACTCCA; CGAAGTAGCTGCTGTTCTTGCTCTGGATGGCCAGCATCTGCTCGTCCACCTCCTTCATGG; ACATGCGGCCCCGGGAACACGTGGCCACCGTCAGGTANCGGCCGTGGCGCGGGTCGCAGG; CGGCCATCATNGTCTTGGCATCGAACATCTGCTGGGTGAGCTCGGNCACGGTCAGGGNCC; CNGTACTGCNTGCTTGCCCGGNCTGTGAGGNGGGCNAANCCCGGCATGAAANTGCAGCGC; NGAANGCACCATGTGACGGCACTTGCGCAGTCACGTGACTGCCCGG;
• Restriction Sites: NotI-NotI
• ACCN: NM_006086
• Insert Size: 1770 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_006086.2, NP_006077.2
• RefSeq Size: 1736 bp
• RefSeq ORF: 1353 bp
• Locus ID: 10381
• UniProt ID: Q13509
• Cytogenetics: 16q24.3
• Domains: tubulin
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS
• Protein Pathways: Gap junction, Pathogenic Escherichia coli infection
• Summary: This gene encodes a class III member of the beta tubulin protein family. Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. This protein is primarily expressed in neurons and may be involved in neurogenesis and axon guidance and maintenance. Mutations in this gene are the cause of congenital fibrosis of the extraocular muscles type 3. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 6. [provided by RefSeq, Oct 2010]; Transcript Variant: This variant (1) encodes the longest isoform (1).