beta III Tubulin (TUBB3) (NM_006086) Human Untagged Clone

CAT#: SC116313

TUBB3 (untagged)-Human tubulin, beta 3 (TUBB3), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: beta III Tubulin
• Synonyms: beta-4; CDCBM; CDCBM1; CFEOM3; CFEOM3A; FEOM3; TUBB4
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC116313 sequence for NM_006086 edited (data generated by NextGen Sequencing)
  ATGAGGGAGATCGTGCACATCCAGGCCGGCCAGTGCGGCAACCAGATCGGGGCCAAGTTC
  TGGGAAGTCATCAGTGATGAGCATGGCATCGACCCCAGCGGCAACTACGTGGGCGACTCG
  GACTTGCAGCTGGAGCGGATCAGCGTCTACTACAACGAGGCCTCTTCTCACAAGTACGTG
  CCTCGAGCCATTCTGGTGGACCTGGAACCCGGAACCATGGACAGTGTCCGCTCAGGGGCC
  TTTGGACATCTCTTCAGGCCTGACAATTTCATCTTTGGTCAGAGTGGGGCCGGCAACAAC
  TGGGCCAAGGGTCACTACACGGAGGGGGCGGAGCTGGTGGATTCGGTCCTGGATGTGGTG
  CGGAAGGAGTGTGAAAACTGCGACTGCCTGCAGGGCTTCCAGCTGACCCACTCGCTGGGG
  GGCGGCACGGGCTCCGGCATGGGCACGTTGCTCATCAGCAAGGTGCGTGAGGAGTATCCC
  GACCGCATCATGAACACCTTCAGCGTCGTGCCCTCACCCAAGGTGTCAGACACGGTGGTG
  GAGCCCTACAACGCCACGCTGTCCATCCACCAGCTGGTGGAGAACACGGATGAGACCTAC
  TGCATCGACAACGAGGCGCTCTACGACATCTGCTTCCGCACCCTCAAGCTGGCCACGCCC
  ACCTACGGGGACCTCAACCACCTGGTATCGGCCACCATGAGCGGAGTCACCACCTCCTTG
  CGCTTCCCGGGCCAGCTCAACGCTGACCTGCGCAAGCTGGCCGTCAACATGGTGCCCTTC
  CCGCGCCTGCACTTCTTCATGCCCGGCTTCGCCCCCCTCACAGCCCGGGGCAGCCAGCAG
  TACCGGGCCCTGACCGTGCCCGAGCTCACCCAGCAGATGTTCGATGCCAAGAACATGATG
  GCCGCCTGCGACCCGCGCCACGGCCGCTACCTGACGGTGGCCACCGTGTTCCGGGGCCGC
  ATGTCCATGAAGGAGGTGGACGAGCAGATGCTGGCCATCCAGAGCAAGAACAGCAGCTAC
  TTCGTGGAGTGGATCCCCAACAACGTGAAGGTGGCCGTGTGTGACATCCCGCCCCGCGGC
  CTCAAGATGTCCTCCACCTTCATCGGGAACAGCACGGCCATCCAGGAGCTGTTCAAGCGC
  ATCTCCGAGCAGTTCACGGCCATGTTCCGGCGCAAGGCCTTCCTGCACTGGTACACGGGC
  GAGGGCATGGACGAGATGGAGTTCACCGAGGCCGAGAGCAACATGAACGACCTGGTGTCC
  GAGTACCAGCAGTACCAGGACGCCACGGCCGAGGAAGAGGGCGAGATGTACGAAGACGAC
  GAGGAGGAGTCGGAGGCCCAGGGCCCCAAGTGA
  
  Clone variation with respect to NM_006086.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006086 unedited
  TCAACATTTGTATACGACTCATATAGGCGGCNCGCGAATTCGCACGAGGCCAGTATGAGG
  GAGATCGTGCACATCCAGGCCGGCCAGTGCGGCAACCAGATCGGGGCCAAGTTCTGGGAA
  GTCATCAGTGATGAGCATGGCATCGACCCCAGCGGCAACTACGTGGGCGACTCGGACTTG
  CAGCTGGAGCGGATCAGCGTCTACTACAACGAGGCCTCTTCTCACAAGTACGTGCCTCGA
  GCCATTCTGGTGGACCTGGAACCCGGAACCATGGACAGTGTCCGCTCAGGGGCCTTTGGA
  CATCTCTTCAGGCCTGACAATTTCATCTTTGGTCAGAGTGGGGCCGGCAACAACTGGGCC
  AAGGGTCACTACACGGAGGGGGCGGAGCTGGTGGATTCGGTCCTGGATGTGGTGCGGAAG
  GAGTGTGAAAACTGCGACTGCCTGCAGGGCTTCCAGCTGACCCACTCGCTGGGGGGCGGC
  ACGGGCTCCGGCATGGGCACGTTGCTCATCAGCAAGGTGCGTGAGGAGTATCCCGACCGC
  ATCATGAACACCTTCAGCGTCGTGCCCTCACCCAAGGTGTCAGACACGGTGGTGGAGCCC
  TACAACGCCACGCTGTCCATCCACCAGCTGGTGGAGAACACGGATGAGACCTACTGCATC
  GACAACGAGGCGCTCTACGACATCTGCTTCCGCACCCTCAAGCTGGCCACGCCCACCTAC
  GGNNGACCTCACCACCTGGTATCGGCCACCATGAGCGGAGTCACCACCTCCTTGCGCTTC
  CNGGGCCAGCTCAACGCTGACCTGCGNCAGCTGGGCCGTCACATGGTGCCCTTCCGCGCC
  TGCACTTCTTATGCCCCGCTTCGCCCCCTCACAGCCCGGGCAGCCAGCAGTCCGGGCCCT
  GACGTGCCCGAGCTCAC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_006086 unedited
  GCCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTATCTGACAGCAATAGA
  TTTATTAAGTATCCCCGAAAATTAAACACAAACCAGTAAAAAACAAAACCGTAAAACGTC
  AGGCCTGGAGCTGCAATAAGACAGAGACAGGAGCAGCTCACACGTGGCCTAGGTGGGGAG
  GACGAGGCCATAAATACTGCAGGAGGGCGGCAAGGGAGCCCTAGGGCGAGGGGAAAGCAG
  GGTGTCGGCAGCAAGATGGCTCCGGGGGTTTAGACACTGCTGGCTTCGGCCCCGGCCGCC
  ACCTGCCTCTCACTCCAGCTGCGAGCAGCTTCACTTGGGGCCCTGGGCCTCCGACTCCTC
  CTCGTCGTCTTCGTACATCTCGCCCTCTTCCTCGGCCGTGGCGTCCTGGTACTGCTGGTA
  CTCGGACACCAGGTCGTTCATGTTGCTCTCGGCCTCGGTGAACTCCATCTCGTCCATGCC
  CTCGCCCGTGTACCAGTGCAGGAAGGCCTTGCGCCGGAACATGGCCGTGAACTGCTCGGA
  GATGCGCTTGAACAGCTCCTGGATGGCCGTGCTGTTCCCGATGAAGGTGGAGGACATCTT
  GAGGCCGCGGNGCGGGATGTCACACACGGCCACCTTCACGTTGTTGGGGGATCCACTCCA
  CGAAGTAGCTGCTGTTCTTGCTCTGGATGGCCAGCATCTGCTCGTCCACCTCCTTCATGG
  ACATGCGGCCCCGGGAACACGTGGCCACCGTCAGGTANCGGCCGTGGCGCGGGTCGCAGG
  CGGCCATCATNGTCTTGGCATCGAACATCTGCTGGGTGAGCTCGGNCACGGTCAGGGNCC
  CNGTACTGCNTGCTTGCCCGGNCTGTGAGGNGGGCNAANCCCGGCATGAAANTGCAGCGC
  NGAANGCACCATGTGACGGCACTTGCGCAGTCACGTGACTGCCCGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_006086
• Insert Size: 1770 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006086.2, NP_006077.2
• RefSeq Size: 1736 bp
• RefSeq ORF: 1353 bp
• Locus ID: 10381
• UniProt ID: Q13509
• Cytogenetics: 16q24.3
• Domains: tubulin
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS
• Protein Pathways: Gap junction, Pathogenic Escherichia coli infection
• Gene Summary: This gene encodes a class III member of the beta tubulin protein family. Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. This protein is primarily expressed in neurons and may be involved in neurogenesis and axon guidance and maintenance. Mutations in this gene are the cause of congenital fibrosis of the extraocular muscles type 3. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 6. [provided by RefSeq, Oct 2010]
  Transcript Variant: This variant (1) encodes the longest isoform (1).