beta III Tubulin (TUBB3) (NM_006086) Human Untagged Clone

SKU
SC116313
TUBB3 (untagged)-Human tubulin, beta 3 (TUBB3), transcript variant 1
$457.00
In Stock*
Specifications
Product Data
Type Human Untagged Clone
Target Symbol beta III Tubulin
Synonyms beta-4; CDCBM; CDCBM1; CFEOM3; CFEOM3A; FEOM3; TUBB4
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF within SC116313 sequence for NM_006086 edited (data generated by NextGen Sequencing)
ATGAGGGAGATCGTGCACATCCAGGCCGGCCAGTGCGGCAACCAGATCGGGGCCAAGTTC
TGGGAAGTCATCAGTGATGAGCATGGCATCGACCCCAGCGGCAACTACGTGGGCGACTCG
GACTTGCAGCTGGAGCGGATCAGCGTCTACTACAACGAGGCCTCTTCTCACAAGTACGTG
CCTCGAGCCATTCTGGTGGACCTGGAACCCGGAACCATGGACAGTGTCCGCTCAGGGGCC
TTTGGACATCTCTTCAGGCCTGACAATTTCATCTTTGGTCAGAGTGGGGCCGGCAACAAC
TGGGCCAAGGGTCACTACACGGAGGGGGCGGAGCTGGTGGATTCGGTCCTGGATGTGGTG
CGGAAGGAGTGTGAAAACTGCGACTGCCTGCAGGGCTTCCAGCTGACCCACTCGCTGGGG
GGCGGCACGGGCTCCGGCATGGGCACGTTGCTCATCAGCAAGGTGCGTGAGGAGTATCCC
GACCGCATCATGAACACCTTCAGCGTCGTGCCCTCACCCAAGGTGTCAGACACGGTGGTG
GAGCCCTACAACGCCACGCTGTCCATCCACCAGCTGGTGGAGAACACGGATGAGACCTAC
TGCATCGACAACGAGGCGCTCTACGACATCTGCTTCCGCACCCTCAAGCTGGCCACGCCC
ACCTACGGGGACCTCAACCACCTGGTATCGGCCACCATGAGCGGAGTCACCACCTCCTTG
CGCTTCCCGGGCCAGCTCAACGCTGACCTGCGCAAGCTGGCCGTCAACATGGTGCCCTTC
CCGCGCCTGCACTTCTTCATGCCCGGCTTCGCCCCCCTCACAGCCCGGGGCAGCCAGCAG
TACCGGGCCCTGACCGTGCCCGAGCTCACCCAGCAGATGTTCGATGCCAAGAACATGATG
GCCGCCTGCGACCCGCGCCACGGCCGCTACCTGACGGTGGCCACCGTGTTCCGGGGCCGC
ATGTCCATGAAGGAGGTGGACGAGCAGATGCTGGCCATCCAGAGCAAGAACAGCAGCTAC
TTCGTGGAGTGGATCCCCAACAACGTGAAGGTGGCCGTGTGTGACATCCCGCCCCGCGGC
CTCAAGATGTCCTCCACCTTCATCGGGAACAGCACGGCCATCCAGGAGCTGTTCAAGCGC
ATCTCCGAGCAGTTCACGGCCATGTTCCGGCGCAAGGCCTTCCTGCACTGGTACACGGGC
GAGGGCATGGACGAGATGGAGTTCACCGAGGCCGAGAGCAACATGAACGACCTGGTGTCC
GAGTACCAGCAGTACCAGGACGCCACGGCCGAGGAAGAGGGCGAGATGTACGAAGACGAC
GAGGAGGAGTCGGAGGCCCAGGGCCCCAAGTGA

Clone variation with respect to NM_006086.3
5' Read Nucleotide Sequence
>OriGene 5' read for NM_006086 unedited
TCAACATTTGTATACGACTCATATAGGCGGCNCGCGAATTCGCACGAGGCCAGTATGAGG
GAGATCGTGCACATCCAGGCCGGCCAGTGCGGCAACCAGATCGGGGCCAAGTTCTGGGAA
GTCATCAGTGATGAGCATGGCATCGACCCCAGCGGCAACTACGTGGGCGACTCGGACTTG
CAGCTGGAGCGGATCAGCGTCTACTACAACGAGGCCTCTTCTCACAAGTACGTGCCTCGA
GCCATTCTGGTGGACCTGGAACCCGGAACCATGGACAGTGTCCGCTCAGGGGCCTTTGGA
CATCTCTTCAGGCCTGACAATTTCATCTTTGGTCAGAGTGGGGCCGGCAACAACTGGGCC
AAGGGTCACTACACGGAGGGGGCGGAGCTGGTGGATTCGGTCCTGGATGTGGTGCGGAAG
GAGTGTGAAAACTGCGACTGCCTGCAGGGCTTCCAGCTGACCCACTCGCTGGGGGGCGGC
ACGGGCTCCGGCATGGGCACGTTGCTCATCAGCAAGGTGCGTGAGGAGTATCCCGACCGC
ATCATGAACACCTTCAGCGTCGTGCCCTCACCCAAGGTGTCAGACACGGTGGTGGAGCCC
TACAACGCCACGCTGTCCATCCACCAGCTGGTGGAGAACACGGATGAGACCTACTGCATC
GACAACGAGGCGCTCTACGACATCTGCTTCCGCACCCTCAAGCTGGCCACGCCCACCTAC
GGNNGACCTCACCACCTGGTATCGGCCACCATGAGCGGAGTCACCACCTCCTTGCGCTTC
CNGGGCCAGCTCAACGCTGACCTGCGNCAGCTGGGCCGTCACATGGTGCCCTTCCGCGCC
TGCACTTCTTATGCCCCGCTTCGCCCCCTCACAGCCCGGGCAGCCAGCAGTCCGGGCCCT
GACGTGCCCGAGCTCAC
3' Read Nucleotide Sequence
>OriGene 3' read for NM_006086 unedited
GCCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTATCTGACAGCAATAGA
TTTATTAAGTATCCCCGAAAATTAAACACAAACCAGTAAAAAACAAAACCGTAAAACGTC
AGGCCTGGAGCTGCAATAAGACAGAGACAGGAGCAGCTCACACGTGGCCTAGGTGGGGAG
GACGAGGCCATAAATACTGCAGGAGGGCGGCAAGGGAGCCCTAGGGCGAGGGGAAAGCAG
GGTGTCGGCAGCAAGATGGCTCCGGGGGTTTAGACACTGCTGGCTTCGGCCCCGGCCGCC
ACCTGCCTCTCACTCCAGCTGCGAGCAGCTTCACTTGGGGCCCTGGGCCTCCGACTCCTC
CTCGTCGTCTTCGTACATCTCGCCCTCTTCCTCGGCCGTGGCGTCCTGGTACTGCTGGTA
CTCGGACACCAGGTCGTTCATGTTGCTCTCGGCCTCGGTGAACTCCATCTCGTCCATGCC
CTCGCCCGTGTACCAGTGCAGGAAGGCCTTGCGCCGGAACATGGCCGTGAACTGCTCGGA
GATGCGCTTGAACAGCTCCTGGATGGCCGTGCTGTTCCCGATGAAGGTGGAGGACATCTT
GAGGCCGCGGNGCGGGATGTCACACACGGCCACCTTCACGTTGTTGGGGGATCCACTCCA
CGAAGTAGCTGCTGTTCTTGCTCTGGATGGCCAGCATCTGCTCGTCCACCTCCTTCATGG
ACATGCGGCCCCGGGAACACGTGGCCACCGTCAGGTANCGGCCGTGGCGCGGGTCGCAGG
CGGCCATCATNGTCTTGGCATCGAACATCTGCTGGGTGAGCTCGGNCACGGTCAGGGNCC
CNGTACTGCNTGCTTGCCCGGNCTGTGAGGNGGGCNAANCCCGGCATGAAANTGCAGCGC
NGAANGCACCATGTGACGGCACTTGCGCAGTCACGTGACTGCCCGG
Restriction Sites NotI-NotI
ACCN NM_006086
Insert Size 1770 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_006086.2, NP_006077.2
RefSeq Size 1736 bp
RefSeq ORF 1353 bp
Locus ID 10381
UniProt ID Q13509
Cytogenetics 16q24.3
Domains tubulin
Protein Families Druggable Genome, ES Cell Differentiation/IPS
Protein Pathways Gap junction, Pathogenic Escherichia coli infection
Summary This gene encodes a class III member of the beta tubulin protein family. Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. This protein is primarily expressed in neurons and may be involved in neurogenesis and axon guidance and maintenance. Mutations in this gene are the cause of congenital fibrosis of the extraocular muscles type 3. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 6. [provided by RefSeq, Oct 2010]
Transcript Variant: This variant (1) encodes the longest isoform (1).
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Citations

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