NFYB (NM_006166) Human Untagged Clone

SKU SC116285

NFYB (untagged)-Human nuclear transcription factor Y, beta (NFYB)

Product Data

• Type: Human Untagged Clone
• Target Symbol: NFYB
• Synonyms: CBF-A; CBF-B; HAP3; NF-YB
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_006166 edited; GAATTCGGCACGAGGCCGCGGCGGAGGGAGGGGAGGCCGAGTCCTGGAAGCGGAGCTCCG; CGCTGGGACTGGTTCCTTCGCAGCCATTTTCTGTCCAACCAAACAGCCGATTGGAGACGG; GAGCCAACCAGGGCTGCATTGGAGGTTGAAATCACAAAGATTAGACACCTTTTTAGATAG; GTGTTCTTCAGCACCACTGACAACACGGTTCTGACAGTATTTCATGACAATGGATGGTGA; CAGTTCTACAACAGATGCTTCTCAACTAGGAATCTCTGCAGACTATATTGGAGGAAGTCA; TTATGTTATACAGCCTCATGATGATACTGAGGACAGCATGAATGATCATGAAGACACAAA; TGGTTCAAAAGAAAGTTTCAGAGAACAAGATATATATCTTCCAATAGCAAACGTGGCTAG; GATAATGAAAAATGCCATACCTCAAACGGGAAAGATTGCAAAAGATGCCAAAGAATGTGT; TCAAGAATGTGTAAGTGAGTTCATCAGTTTTATAACATCTGAAGCAAGTGAAAGGTGCCA; TCAAGAGAAACGGAAAACAATCAATGGAGAAGATATTCTCTTTGCTATGTCTACTTTAGG; CTTTGACAGTTATGTGGAACCTCTGAAATTATACCTTCAGAAATTCAGAGAGGCTATGAA; AGGAGAAAAGGGAATTGGTGGAGCAGTCACAGCTACAGATGGACTAAGTGAAGAGCTTAC; AGAGGAGGCATTTACTAACCAGTTACCAGCTGGCTTAATAACCACAGACGGTCAACAACA; AAATGTTATGGTTTACACAACATCATATCAACAGATTTCTGGTGTTCAGCAAATTCAGTT; TTCATGATCTGAAGAAATGATGGAATGGGGAGTGTAGAGAAATGAGAGTCTGTATGATTC; TGGAACAGAGACATCAGAAGGAAAGACTGGTGAAAAGATGTATCTTTGTATATTAATAGC; TGTAATGTAGCTTCCTGATGCTTGACTAATTGAGGTGTTAATTCTGACTTGAGAATCTTT; TTCATGAATGATTTTAAAGAAAAATTTGGATTTTAAAGGTATTAAAATATTTTTGTTTTG; TACGAGAGTTTGTTGCTCTGTATGACTCCTGTATGCATTGTATATTGCAATTTATTACTG; TCAGAGATTTGTAGACAGTTTCTTATTTTCATATTGAATCATGTTACTTTTGTAATTCAA; GTAAGCGGCTGGGTTAATTCATGATGTTTGCCCTTTTAATAAAATATAAGGGTAGAGTTC; ATTTTGAATGCAAGTTGCCTTTATTATAAATTTGAGTTTGTCTTGGTTATACCTTGCATG; ATAACCTAGCTAGATTTCTAGCATTTGCTGTATTTATTAAAATTATTATTTTTTTGGTAA; AACATTAATAGTTTAAGCAGCATCATTTTTTTAAAAAATGTAATTGAATAAGTGTGAATG; CAGAAGCAAATATTGTCTGCCCTGTTAAACTTGGTGCCCATTAACAGTGTTTACACTGTT; CATCGTGCCTGTTAATGTAGTTTTAGTTACTGGAGCTTTTTTAAGACTAGATTTGGTTTT; GAGTTACATTTTTAAGAATGTGGGAATATATTTAAGTTTAATGTAGTCCTAGTGCTCTTG; AAATGGTGCCCCTTTCATTTGGTACATGATTTTTTTTCAAATCATATCTTCAAGTACTAT; AGTATTCTCTTACAGAAGAGGAGTTTTATAGXXXXXXXXXXXXXXXXXXXXXXXXXXXXX; XGTCTTCATTTTACCTTTTTAATTGAAATGTCAAGTTTCCTGTTACACTATGGAAACCAA; GAAACATCAGACATCATTGCGTGTACAGACCTTTTGCATGGGTGAGTGGATGAAATGGAG; AACAGAGTGAGTGCTGTGAACGGTGTGAAATAGAAGCCAACTTCTAGTATGCTGTCTTCA; TCTCTGCAATAAACTAAACGTAAATAATGTAACTTTGTATAATTTAGTAGTATGTACTTG; GATTGTTTCTTTTTGAAAAGTGAGGATTTGTATTTTCTCATCTATTCTTTTTGGTGTTAA; TTAACTTGAGGGATTTCTTTTTTAAGTTTTTAATATCAAAAATTGCTATTGTATAGTTTG; CCTAAAAGGAAGTTGGAGTGAATATCATCTTTGAAGGACACTCAAATTTATAAGGTAGAA; GCAATTTCCCAGATATAGGCTGAACAGAAACATTTACTTTGCCAGAAGGAGCCTTTAGGA; GTATATGTGGGCTAATCAGAGGTTAATAATGTCCTCATCTCCTACTCTCCATTTAAAAAA; CAAACTTCATTGTAGTAAATTCAAATAGGAAAAAATGGGGGAGTAGTTAAGCAAAATCAA; CAGTCATCCTACTATGTTGAAAGAACCAATGTTAATATTTTGATAAATATCATTTGTCCT; TTTACTTTGCTAAATATGTATATTTTTATAAAAATGGGCTCATATTGTGCATACCATTTT; GTAACCTGCTTTTTTCACTTAACAATATATTGGGAACATCTTTAAAAAAAAAAAAAAAAA; AAAACTCGAC;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006166 unedited; AGGTCGAATTTGTATACGACTCCTATAGGCGGCNCGCGATTCGGCACGAGGCCGCGGCGG; AGGGAGGGGAGGCCGAGTCCTGGAAGCGGAGCTCCGCGCTGGGACTGGTTCCTTCGCAGC; CATTTTCTGTCCAACCAAACAGCCGATTGGAGACGGGAGCCAACCAGGGCTGCATTGGAG; GTTGAAATCACAAAGATTAGACACCTTTTTAGATAGGTGTTCTTCAGCACCACTGACAAC; ACGGTTCTGACAGTATTTCATGACAATGGATGGTGACAGTTCTACAACAGATGCTTCTCA; ACTAGGAATCTCTGCAGACTATATTGGAGGAAGTCATTATGTTATACAGCCTCATGATGA; TACTGAGGACAGCATGAATGATCATGAAGACACAAATGGTTCAAAAGAAAGTTTCAGAGA; ACAAGATATATATCTTCCAATAGCAAACGTGGCTAGGATAATGAAAAATGCCATACCTCA; AACGGGAAAGATTGCAAAAGATGCCAAAGAATGTGTTCAAGAATGTGTAAGTGAGTTCAT; CAGTTTTATAACATCTGAAGCAAGTGAAAGGTGCCATCAAGAGAAACGGAAAACAATCAA; TGGAGAAGATATTCTCTTTGCTATGTCTACTTTAGGCTTTGACAGTTATGTGGAACCTCT; GAAATTATACCTTCAGAAATTCAGAGAGGCTATGAAAGGAGAAAAGGGAATTGGGGGAGC; AGTCACAGCTACAGATGGACTAAGTGAAGAGCTTACAGAGGAGGCATTTACTAACCAGTT; ACCAGCTGGCTTAATAACCACAGACGGTCACAACAAATGTTAATGGTTTACACAACATCA; TATAACAGATTTTTTNTGTTCACCANATTCAGTTTCATGATCTGAACAATGATGAAAGGG; GGGAGTGT;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_006166 unedited; ACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTAAAAATGTTCCCAA; TATATTGTTAAGTGAAAAAAGCGGTTACAAAATGGTATGCACAATATGAGCCCATTTTTA; TAAAAATATACATATTTAGCAAAGTAAAAGGACAAATGATATTTATCAAAATATTAACAT; TGGTTCTTTCAACATAGTAGGATGACTGTTGATTTTGCTTAACTACTCCCCCATTTTTTC; CTATTTGAATTTACTACAATGAAGTTTGTTTTTTAAATGGAGAGTAGGAGATGAGGACAT; TATTAACCTCTGATTAGCCCACATATACTCCTAAAGGCTCCTTCTGGCAAAGTAAATGTT; TCTGTTCAGCCTATATCTGGGAAATTGCTTCTACCTTATAAATTTGAGTGTCCTTCAAAG; ATGATATTCACTCCAACTTCCTTTTAGGCAAACTATACAATAGCAATTTTTGATATTAAA; AACTTAAAAAAGAAATCCCTCAAGTTAATTAACACCAAAAAGAATAGATGAGAAAATACA; AATCCTCACTTTTCAAAAAGAAACAATCCAAGTACATACTACTAAATTATACAAAGTTAC; ATTATTTACGTTTAGTTTATTGCAGAGATGAAGACAGCATACTAGAAGTTGGCTTCTATT; TCACACCGTTCACAGCACTCACTCTGTTCTCCATTTCATCCACTCACCCATGCAAAAGGT; CTGTACACCCAATGATGCCTGATGGTTCTTGCGTTCCATAGCGCAACAAGAAAACTCGAC; ATTTCAATTATAAAGGGTAAATGAAGACCTTAACCATCANACTATTAAACTGCTCTTNTG; TAAGAGGATCCTATATTACTTGAAAATTTAATTGAAAAAAAATCATGTCCCAAATGAAGG; GGCCCCTTTCAGAGCCTAGGACTACCTTAACTTAATTATT;
• Restriction Sites: NotI-NotI
• ACCN: NM_006166
• Insert Size: 2850 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_006166.3, NP_006157.1
• RefSeq Size: 3482 bp
• RefSeq ORF: 624 bp
• Locus ID: 4801
• UniProt ID: P25208
• Cytogenetics: 12q23.3
• Domains: CBFD_NFYB_HMF
• Protein Families: Transcription Factors
• Protein Pathways: Antigen processing and presentation
• Summary: The protein encoded by this gene is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds with high specificity to CCAAT motifs in the promoter regions in a variety of genes. This gene product, subunit B, forms a tight dimer with the C subunit, a prerequisite for subunit A association. The resulting trimer binds to DNA with high specificity and affinity. Subunits B and C each contain a histone-like motif. Observation of the histone nature of these subunits is supported by two types of evidence; protein sequence alignments and experiments with mutants. [provided by RefSeq, Jul 2008]