LASP1 (NM_006148) Human Untagged Clone

SKU SC116276

LASP1 (untagged)-Human LIM and SH3 protein 1 (LASP1)

Product Data

• Type: Human Untagged Clone
• Target Symbol: LASP1
• Synonyms: Lasp-1; MLN50
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC116276 sequence for NM_006148 edited (data generated by NextGen Sequencing); ATGAACCCCAACTGCGCCCGGTGCGGCAAGATCGTGTATCCCACGGAGAAGGTGAACTGT; CTGGATAAGTTCTGGCATAAAGCATGCTTCCATTGCGAGACCTGCAAGATGACACTGAAC; ATGAAGAACTACAAGGGCTACGAGAAGAAGCCCTACTGCAACGCACACTACCCCAAGCAG; TCCTTCACCATGGTGGCGGACACCCCGGAAAACCTTCGCCTCAAGCAACAGAGTGAGCTC; CAGAGTCAGGTGCGCTACAAGGAGGAGTTTGAGAAGAACAAGGGCAAAGGTTTCAGCGTA; GTGGCAGACACGCCCGAGCTCCAGAGAATCAAGAAGACCCAGGACCAGATCAGTAACATA; AAATACCATGAGGAGTTTGAGAAGAGCCGCATGGGCCCTAGCGGGGGCGAGGGCATGGAG; CCAGAGCGTCGGGATTCACAGGACGGCAGCAGCTACCGGCGGCCCCTGGAGCAGCAGCAG; CCTCACCACATCCCGACCAGTGCCCCGGTTTACCAGCAGCCCCAGCAGCAGCCGGTGGCC; CAGTCCTATGGTGGCTACAAGGAGCCTGCAGCCCCAGTCTCCATACAGCGCAGCGCCCCA; GGTGGTGGCGGGAAGCGGTACCGCGTGGTGTATGACTACAGCGCCGCCGACGAGGACGAG; GTCTCCTTCCAGGACGGGGACACCATCGTCAACGTGCAGCAGATCGACGACGGCTGGATG; TACGGGACGGTGGAGCGCACCGGCGACACGGGGATGCTGCCGGCCAACTACGTGGAGGCC; ATCTGA; ; Clone variation with respect to NM_006148.2; 626 c=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006148 unedited; GTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGCTCCAGCCGCCGTCGC; TGCTGCCTGTGTAGTTGCAGCCGCGGCCGCCTCCCGCCAGCTCGCCTCGGGGAACAGGAC; GCGCGTGAGCTCAGGCGTCCCCGCCCCAGCTTTTCTCGGAACCATGAACCCCAACTGCGC; CCGGTGCGGCAAGATCGTGTATCCCACGGAGAAGGTGAACTGTCTGGATAAGTTCTGGCA; TAAAGCATGCTTCCATTGCGAGACCTGCAAGATGACACTGAACATGAAGAACTACAAGGG; CTACGAGAAGAAGCCCTACTGCAACGCACACTACCCCAAGCAGTCCTTCACCATGGTGGC; GGACACCCCGGAAAACCTTCGCCTCAAGCAACAGAGTGAGCTCCAGAGTCAGGTGCGCTA; CAAGGAGGAGTTTGAGAAGAACAAGGGCAAAGGTTTCAGCGTAGTGGCAGACACGCCCGA; GCTCCAGAGAATCAAGAAGACCCAGGACCAGATCAGTAACATAAAATACCATGAGGAGTT; TGAGAAGAGCCGCATGGGCCCTAGCGGGGGCGAGGGCATGGAGCCAGAGCGTCGGGATTC; ACAGGACGGCAGCAGCTACCGGCGGCCCCTGGAGCAGCAGCAGCCTCACCACATCCCGAC; CAGTGCCCCGGTTTACCAGCAGCCCCAGCAGCAGCCGGTGGCCCAGTTCTATGGTGGGCT; ACAGGAGCCTGCAGCCCCAGTCTCCATACAGCGCAGCGCCCCAGGTGGGTGGCGGGAAAG; CGTACCGGGTGGTGTATGACTACCAGCGCCGNCGACGAAGACGAGGTCTTCTTNCAGNAC; GGGGACACCATCGNTCACGTGCANCAAATCNACNACGGTTGGATGTCCGGGACGGTGGAC; N;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_006148 unedited; TCTAGAGTCGAGTTTTTTTTTTTTTTTTTTAAAAATATACTTTTATTTGAGTCACCAGGA; GAAAGATTCACTTGTGGTTCACTTCAAACGTTCAGAATCATAACAGGCCAGAAAGGTTTG; ATCCCGAGCACAAGCCCACGAGGGAGGGGACCAAAACAGACCAAAATGAGACAACAACCC; CATATAAAAAGATGAACTGGCGGCTTCACACACTCACACACATACACATACACACGGATG; AAATGTTTGGACAGAGGCAAATTTCACGTGGTCATTTCTGTTTCTTTTTAAATACATGTT; TGTAGGGTGGTATTTTGTTTTTTCCAGCTATAAAAAAAGGCCCAAAAGTGCATGTGTGAG; GGGGGAAAGGCANAAATTAAGCAATAAAGTCATTTTCCCTGGAGGGACATGAGAGGGAGA; AAACAGGAGGCAGTGCTGGGAGAACGCACTCTCCTCACCACTGGGCTTCTTGTTATTCTT; AGTATTGGTCCACAAAAGTTATATTCAACTTCTAGCTTTGATGCCTTCTTTCCTGAGATT; AAATGAGCTGAAAGACCTCTGTGAACTGTAGAGAAGACCAGGGGCTTACCCCAACCCAGC; CCAGCCCTCAAGCCTCTGTGCTCTCTAAGGATGAACGTTTTGGTAAAGACAGACGCCCAG; CACCCCCCGGTACTAAGTACCATTGTGACAGGTCCTCCTCTCTCTGGGGTGGGAAAAAAA; CGCCCCTATTTTAGACAGGACACGAAGGGAGGCCTCGCCCAGATCCCTGATGCTTTGCCT; AATTGGAGGAAATTACCTGATGAAACACGCTGTTTTGTTGGCCTCCTTCACTCACATTTC; CAACAAGGTCTTAAAGGGTGGGCCCGACCTGGCCCCAATCCACTGGCTTGAAACAAGGAA; CAAAGCTACCGCCCCCAATCCAAAAACCTGCCCCCCACCCCCAAGCCATAAAGAATCCAT; GCCCCATACTGCGCCAAGAAAGAAGCTACAAGCCCCCC;
• Restriction Sites: NotI-NotI
• ACCN: NM_006148
• Insert Size: 4310 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_006148.1, NP_006139.1
• RefSeq Size: 3846 bp
• RefSeq ORF: 786 bp
• Locus ID: 3927
• UniProt ID: Q14847
• Cytogenetics: 17q12
• Domains: LIM, NEBU, SH3
• Protein Families: Druggable Genome
• Summary: This gene encodes a member of a subfamily of LIM proteins, characterized by a LIM motif and a domain of Src homology region 3, and also a member of the nebulin family of actin-binding proteins. The encoded protein is a cAMP and cGMP dependent signaling protein and binds to the actin cytoskeleton at extensions of the cell membrane. The encoded protein has been linked to metastatic breast cancer, hematopoetic tumors such as B-cell lymphomas, and colorectal cancer. [provided by RefSeq, Oct 2012]; Transcript Variant: This variant (1) encodes the longest isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.