CGK2 (PRKG2) (NM_006259) Human Untagged Clone

SKU SC116254

PRKG2 (untagged)-Human protein kinase, cGMP-dependent, type II (PRKG2)

Product Data

• Type: Human Untagged Clone
• Target Symbol: CGK2
• Synonyms: cGK2; cGKII; PKG2; PRKGR2
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_006259 edited; ATGGGAAATGGTTCAGTGAAACCTAAACATTCTAAGCACCCAGATGGACACTCTGGGAAC; CTCACCACTGATGCTCTGCGGAACAAGGTGACAGAGCTGGAGAGAGAGTTGAGGAGGAAG; GATGCTGAGATCCAGGAGCGGGAGTACCATTTGAAGGAGCTGCGGGAGCAGCTGTCGAAG; CAGACTGTGGCCATTGCTGAACTCACAGAGGAGCTCCAGAACAAGTGCATCCAGCTGAAC; AAGCTGCAGGATGTGGTGCATATGCAGGGAGGAAGCCCGCTTCAGGCCTCTCCAGATAAA; GTGCCTCTTGAGGTCCACCGGAAGACCTCTGGATTGGTCTCTCTCCATAGCAGGAGGGGA; GCAAAGGCTGGCGTGTCTGCTGAGCCAACAACCCGGACCTATGACCTGAACAAACCCCCT; GAATTTTCCTTTGAGAAAGCAAGAGTCAGAAAAGACTCCAGTGAGAAGAAGCTCATTACA; GATGCCCTTAATAAAAATCAGTTTCTGAAAAGACTGGATCCTCAGCAGATCAAAGACATG; GTGGAATGCATGTATGGGAGAAACTATCAGCAAGGGAGTTACATTATTAAGCAAGGAGAA; CCAGGAAACCATATCTTTGTGCTGGCAGAGGGTCGACTAGAGGTGTTCCAAGGGGAGAAA; TTGCTGTCCTCCATCCCTATGTGGACCACATTTGGGGAGCTTGCCATTTTATACAATTGT; ACAAGGACTGCCTCTGTGAAAGCTATTACCAATGTTAAAACATGGGCACTAGATCGAGAG; GTATTCCAGAATATAATGAGGAGGACAGCCCAAGCTAGAGATGAACAATACAGAAACTTC; CTCAGAAGTGTATCCTTGCTGAAGAATTTACCTGAAGATAAATTAACCAAGATCATTGAC; TGCTTGGAAGTGGAATACTATGACAAAGGAGATTACATCATTAGAGAGGGCGAGGAAGGA; AGTACCTTTTTCATTTTGGCAAAAGGAAAGGTAAAAGTAACACAGAGCACAGAAGGCCAT; GATCAACCACAGCTGATAAAAACACTGCAGAAAGGAGAATACTTTGGAGAAAAAGCTCTT; ATCAGTGATGATGTCAGGTCAGCTAACATTATTGCTGAAGAAAATGATGTTGCATGCCTG; GTTATAGATCGAGAAACATTCAACCAAACTGTCGGTACATTTGAAGAGCTGCAAAAATAC; CTTGAAGGATATGTGGCAAACCTGAACCGTGATGATGAAAAAAGACATGCGAAGCGGTCC; ATGTCTAACTGGAAGCTGTCCAAAGCACTCTCTCTGGAAATGATTCAGCTGAAGGAGAAG; GTGGCCAGATTTTCCTCATCATCCCCATTCCAGAACCTTGAGATTATTGCAACACTGGGC; GTTGGTGGGTTCGGAAGAGTTGAGCTTGTTAAAGTAAAAAATGAGAATGTTGCTTTTGCT; ATGAAGTGTATAAGGAAGAAGCACATAGTTGACACCAAGCAGCAGGAGCATGTCTACTCA; GAGAAGAGGATCCTAGAGGAGCTGTGCTCTCCATTCATTGTGAAATTATATCGTACTTTC; AAGGACAATAAGTATGTATACATGCTTCTGGAGGCCTGCTTAGGTGGGGAGCTCTGGAGT; ATATTAAGGGACAGAGGCAGCTTTGATGAACCCACCTCCAAATTCTGCGTTGCTTGTGTG; ACAGAAGCATTTGATTACCTGCATCGACTAGGTATTATCTACAGAGACTTGAAACCAGAA; AACTTAATTCTAGATGCTGAGGGTTACCTTAAATTGGTTGACTTTGGATTTGCGAAGAAA; ATAGGGTCTGGACAGAAAACATGGACATTCTGTGGGACTCCAGAATATGTAGCTCCTGAA; GTCATTCTCAACAAGGGACATGACTTCAGTGTGGATTTCTGGTCACTGGGAATTCTAGTG; TATGAGCTCCTAACGGGCAACCCACCCTTTTCTGGGGTTGACCAAATGATGACCTACAAT; TTGATTCTCAAAGGAATTGAAAAAATGGATTTTCCCAGGAAGATAACACGACGACCTGAG; GATTTGATTCGGAGGCTTTGCAGGCAAAATCCAACAGAAAGGCTGGGAAATCTGAAGAAT; GGAATAAATGACATTAAGAAACACAGGTGGTTAAATGGTTTTAATTGGGAGGGACTGAAA; GCACGGAGCCTTCCATCACCTTTGCAAAGAGAGCTCAAGGGACCCATAGATCACAGCTAC; TTTGACAAATATCCTCCTGAAAAGGGAATGCCTCCAGATGAGCTATCAGGCTGGGATAAA; GACTTCTGA;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006259 unedited; GGCACCAGCCAGAGTGAACTATGGTTTTTGGTTGCCTTCAAAGATAATTAGGTCCCTGAG; CAAAATGGGAAATGGTTCAGTGAAACCTAAACATTCTAAGCACCCAGATGGACACTCTGG; GAACCTCACCACTGATGCTCTGCGGAACAAGGTGACAGAGCTGGAGAGAGAGTTGAGGAG; GAAGGATGCTGAGATCCAGGAGCGGGAGTACCATTTGAAGGAGCTGCGGGAGCAGCTGTC; GAAGCAGACTGTGGCCATTGCTGAACTCACAGAGGAGCTCCAGAACAAGTGCATCCAGCT; GAACAAGCTGCAGGATGTGGTGCATATGCAGGGAGGAAGCCCGCTTCAGGCCTCTCCAGA; TAAAGTGCCTCTTGAGGTCCACCGGAAGACCTCTGGATTGGTCTCTCTCCATAGCAGGAG; GGGAGCAAAGGCTGGCGTGTCTGCTGAGCCAACAACCCGGACCTATGACCTGAACAAACC; CCCTGAATTTTCCTTTGAGAAAGCAAGAGTCAGAAAAGACTCCAGTGAGAAGAAGCTCAT; TACAGATGCCCTTAATAAAAATCAGTTTCTGAAAAGACTGGATCCTCAGCAGATCAAAGA; CATGGTGGAATGCATGTATGGGAGAAACTATCAGNCAGGGAGTTACATTATTAAGCAAGG; AGAACCAGGANACCATATCTTTGTGCTGGCAGAGGGTCGACTAGAGGTGTTCCAAGGGGA; GAAATTGCTGTCCTCCATCCCTATGTGGACCACATTTGGGGAGCTTGCCATTTTATACAA; TTGTACAAGGACTGCCTT;
• Restriction Sites: NotI-NotI
• ACCN: NM_006259
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_006259.1, NP_006250.1
• RefSeq Size: 3328 bp
• RefSeq ORF: 2289 bp
• Locus ID: 5593
• UniProt ID: Q13237
• Cytogenetics: 4q21.21
• Domains: cNMP, pkinase, S_TKc, S_TK_X, TyrKc
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Gap junction, Long-term depression, Olfactory transduction
• Summary: This gene encodes a protein that belongs to the serine/threonine protein kinase family of proteins. The encoded protein binds to and inhibits the activation of several receptor tyrosine kinases. The membrane-bound protein is a regulator of intestinal secretion, bone growth and renin secretion. Alternate splicing results in multiple transcript variants encoding distinct isoforms whose regulatory N-termini differ in length but whose C-terminal catalytic domains are identical. [provided by RefSeq, May 2018]; Transcript Variant: This variant (1) encodes the longest isoform (a). Variants 1 and 7 encode the same isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.